Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	2,562,703	C→G	15.5%	G458G (GGG→GGC)	lldP ←	L‑lactate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	2,562,703	0	C	G	15.5%	170.4 / 20.8	71	G458G (GGG→GGC)	lldP	L‑lactate permease
Reads supporting (aligned to +/- strand):  ref base C (20/40);  new base G (8/3);  total (28/43)
Fisher's exact test for biased strand distribution p-value = 1.99e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

CAGAGACGCCAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCA  >  Exported/2562633‑2562773                                                                       |                                                                       cAGAGACGCCAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGAc                                                                        >  1:2162155/1‑71 (MQ=255)  agagACGCCAATTTGTTGTGCTGCGGTGCCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGAcc                                                                       >  1:4155344/1‑71 (MQ=255)    agaCGCCAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCg                                                                     <  1:4083833/71‑1 (MQ=255)      aCGCCAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGt                                                                   <  1:2688111/71‑1 (MQ=255)        gCCAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTc                                                                  <  1:4373999/70‑1 (MQ=255)         ccAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAg                                                                <  1:5038296/71‑1 (MQ=255)         ccAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAg                                                                <  1:5506855/71‑1 (MQ=255)         ccAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAg                                                                <  1:2247352/71‑1 (MQ=255)          cAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAgg                                                               <  1:1007519/71‑1 (MQ=255)          cAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAg                                                                >  1:1724878/1‑70 (MQ=255)           aaTTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAgg                                                               >  1:4393023/1‑70 (MQ=255)           aaTTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAgg                                                               >  1:3127479/1‑70 (MQ=255)            aTTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGaa                                                             <  1:2489262/71‑1 (MQ=255)             tttGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGaa                                                             >  1:5026726/1‑70 (MQ=255)               tgttgtGCTGCGGTGGCTTGCAGCGCGGCTAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATa                                                           <  1:281282/70‑1 (MQ=255)               tgttgtGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATa                                                           <  1:2174298/70‑1 (MQ=255)               tgttgtGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATAc                                                          >  1:4025032/1‑71 (MQ=255)                    tgctgcGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCcca                                                     <  1:2720917/71‑1 (MQ=255)                     gctgcGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAg                                                                >  1:4231222/1‑59 (MQ=255)                     gctgcGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAg                                                                >  1:4986613/1‑59 (MQ=255)                       tgcGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAgg                                                               <  1:379085/58‑1 (MQ=255)                       tgcGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAgg                                                               <  1:552893/58‑1 (MQ=255)                       tgcGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGaa                                                             <  1:829364/60‑1 (MQ=255)                       tgcGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGaa                                                             <  1:1714504/60‑1 (MQ=255)                        gcGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCccagc                                                   >  1:1995086/1‑69 (MQ=255)                           gTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCagccagcc                                              >  1:3022863/1‑71 (MQ=255)                            tGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGt                                                                   <  1:3863161/49‑1 (MQ=255)                            tGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCagccagcc                                              <  1:1658321/70‑1 (MQ=255)                             ggCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCagccagcc                                              >  1:3255476/1‑69 (MQ=255)                                ttGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGAcc                                                                       <  1:5156631/41‑1 (MQ=255)                                  gCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGa                                         <  1:915637/69‑1 (MQ=255)                                   cAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAAc                                       <  1:4768465/70‑1 (MQ=255)                                   cAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCAGTCAGGAATACCCCCAGCCAGCCGAGGAAc                                       <  1:2596432/70‑1 (MQ=255)                                    aGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACgg                                     >  1:383742/1‑71 (MQ=255)                                        cggcgAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCagccagcc                                              <  1:324851/58‑1 (MQ=255)                                        cggcgAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCg                                   <  1:5575591/69‑1 (MQ=255)                                          gcgAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCcca                                                     >  1:3175738/1‑49 (MQ=255)                                          gcgAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGagaa                               <  1:2752940/71‑1 (MQ=255)                                            gAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGagaaga                             <  1:699560/71‑1 (MQ=255)                                            aaaCAGGGCGTTAGAGGAGGTGTCCGAGCCGGTAAGGaa                                                             >  1:2414073/2‑39 (MQ=25)                                             aaCAGGGCGTTAGATGAGGTATCCGACCCGGTCAgg                                                               <  1:5387027/36‑1 (MQ=255)                                             aaCAGGGCGTTAGATGAGGTATCCGACCCGGTCAgg                                                               <  1:1439776/36‑1 (MQ=255)                                             aaCAGGGCGTTAGAGGAGGTGTCCGAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAgaagaa                            <  1:1829411/71‑1 (MQ=255)                                             aaCAGGGCGTTAGAGGAGGTGTCCGAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAgaagaa                            <  1:1830777/71‑1 (MQ=255)                                              aCAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGaagaag                           >  1:3731184/1‑71 (MQ=255)                                              aCAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGaagaag                           >  1:170310/1‑71 (MQ=255)                                                aGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGt                         <  1:1533219/71‑1 (MQ=255)                                                aGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGt                         <  1:4194809/71‑1 (MQ=255)                                                aGGGCGTTAGAGGAGGTGTCCGAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACgg                                     >  1:4234613/1‑59 (MQ=255)                                                aGGGCGTTAGAGGAGGTGTCCGAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAgaagaa                            >  1:1320443/1‑68 (MQ=255)                                                 gggCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTg                        <  1:260290/71‑1 (MQ=255)                                                 gggCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTg                        <  1:3965777/71‑1 (MQ=255)                                                 gggCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCACCAGCCAGCCGAGGAACGGCGagaaga                             <  1:4815674/66‑1 (MQ=255)                                                   gCGTTAGAGGAGGTGTCCGAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGaa                      >  1:4260842/1‑71 (MQ=255)                                                   gCGTTAGAGGAGGTGTCCGAGCCGCTAAGCAATACGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGaa                      >  1:3230097/1‑71 (MQ=255)                                                      ttAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGagaag                              <  1:5207988/60‑1 (MQ=255)                                                      ttAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAAt                     <  1:5457942/69‑1 (MQ=255)                                                      ttAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAAt                     <  1:3331589/69‑1 (MQ=255)                                                      ttAGAGGAGGTGTCCGAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGaacat                   <  1:4839846/71‑4 (MQ=255)                                                        agagGAGGTGTCCGAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACggt                                    >  1:4210752/1‑51 (MQ=19)                                                        aGATGAGGTATCCGACCCGGTCAGGAATACCCCcagccagc                                               >  1:4126596/1‑41 (MQ=255)                                                        aGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCAt                 >  1:3162993/1‑71 (MQ=255)                                                           tGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGa               <  1:1856228/70‑1 (MQ=255)                                                            gAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGAcc             >  1:2169980/1‑71 (MQ=255)                                                             aGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGt                         <  1:3951219/58‑1 (MQ=255)                                                              ggTGTCCGAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGagaa                               >  1:5360147/1‑51 (MQ=19)                                                                  tCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGTGagaag                              >  1:3513565/1‑48 (MQ=255)                                                                  tCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGagaag                              >  1:140899/1‑48 (MQ=255)                                                                    cGAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGaac                     >  1:1739630/1‑54 (MQ=255)                                                                    cGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTgcg     >  1:4110773/1‑71 (MQ=255)                                                                     gACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTgcgc    <  1:4141274/71‑1 (MQ=255)                                                                     gACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTgcgc    <  1:2511757/71‑1 (MQ=255)                                                                     gACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTgcgc    <  1:2298358/71‑1 (MQ=255)                                                                      aCCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGcc   <  1:3007749/71‑1 (MQ=255)                                                                       cccGGTCAGGAATACCCCCAGCCAGACGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCa  <  1:4808242/71‑1 (MQ=255)                                                                       |                                                                       CAGAGACGCCAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCA  >  Exported/2562633‑2562773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑