Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	2,562,709	C→A	14.9%	L456L (CTG→CTT)	lldP ←	L‑lactate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	2,562,709	0	C	A	14.9%	209.8 / 14.5	73	L456L (CTG→CTT)	lldP	L‑lactate permease
Reads supporting (aligned to +/- strand):  ref base C (21/41);  new base A (8/3);  total (29/44)
Fisher's exact test for biased strand distribution p-value = 2.10e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGC  >  Exported/2562640‑2562777                                                                      |                                                                     gCCAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTc                                                                      <  1:4373999/70‑1 (MQ=255)  ccAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAg                                                                    <  1:5038296/71‑1 (MQ=255)  ccAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAg                                                                    <  1:2247352/71‑1 (MQ=255)  ccAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAg                                                                    <  1:5506855/71‑1 (MQ=255)   cAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAgg                                                                   <  1:1007519/71‑1 (MQ=255)   cAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAg                                                                    >  1:1724878/1‑70 (MQ=255)    aaTTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAgg                                                                   >  1:4393023/1‑70 (MQ=255)    aaTTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAgg                                                                   >  1:3127479/1‑70 (MQ=255)     aTTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGaa                                                                 <  1:2489262/71‑1 (MQ=255)      tttGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGaa                                                                 >  1:5026726/1‑70 (MQ=255)        tgttgtGCTGCGGTGGCTTGCAGCGCGGCTAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATa                                                               <  1:281282/70‑1 (MQ=255)        tgttgtGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATa                                                               <  1:2174298/70‑1 (MQ=255)        tgttgtGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATAc                                                              >  1:4025032/1‑71 (MQ=255)             tgctgcGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCcca                                                         <  1:2720917/71‑1 (MQ=255)              gctgcGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAg                                                                    >  1:4231222/1‑59 (MQ=255)              gctgcGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAg                                                                    >  1:4986613/1‑59 (MQ=255)                tgcGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAgg                                                                   <  1:379085/58‑1 (MQ=255)                tgcGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAgg                                                                   <  1:552893/58‑1 (MQ=255)                tgcGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGaa                                                                 <  1:829364/60‑1 (MQ=255)                tgcGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGaa                                                                 <  1:1714504/60‑1 (MQ=255)                 gcGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCccagc                                                       >  1:1995086/1‑69 (MQ=255)                    gTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCagccagcc                                                  >  1:3022863/1‑71 (MQ=255)                     tGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCagccagcc                                                  <  1:1658321/70‑1 (MQ=255)                      ggCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCagccagcc                                                  >  1:3255476/1‑69 (MQ=255)                           gCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGa                                             <  1:915637/69‑1 (MQ=255)                            cAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAAc                                           <  1:4768465/70‑1 (MQ=255)                            cAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCAGTCAGGAATACCCCCAGCCAGCCGAGGAAc                                           <  1:2596432/70‑1 (MQ=255)                             aGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACgg                                         >  1:383742/1‑71 (MQ=255)                                 cggcgAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCagccagcc                                                  <  1:324851/58‑1 (MQ=255)                                 cggcgAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCg                                       <  1:5575591/69‑1 (MQ=255)                                   gcgAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCcca                                                         >  1:3175738/1‑49 (MQ=255)                                   gcgAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGagaa                                   <  1:2752940/71‑1 (MQ=255)                                     gAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGagaaga                                 <  1:699560/71‑1 (MQ=255)                                     aaaCAGGGCGTTAGAGGAGGTGTCCGAGCCGGTAAGGaa                                                                 >  1:2414073/2‑39 (MQ=25)                                      aaCAGGGCGTTAGATGAGGTATCCGACCCGGTCAgg                                                                   <  1:1439776/36‑1 (MQ=255)                                      aaCAGGGCGTTAGATGAGGTATCCGACCCGGTCAgg                                                                   <  1:5387027/36‑1 (MQ=255)                                      aaCAGGGCGTTAGAGGAGGTGTCCGAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAgaagaa                                <  1:1829411/71‑1 (MQ=255)                                      aaCAGGGCGTTAGAGGAGGTGTCCGAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAgaagaa                                <  1:1830777/71‑1 (MQ=255)                                       aCAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGaagaag                               >  1:170310/1‑71 (MQ=255)                                       aCAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGaagaag                               >  1:3731184/1‑71 (MQ=255)                                         aGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGt                             <  1:1533219/71‑1 (MQ=255)                                         aGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGt                             <  1:4194809/71‑1 (MQ=255)                                         aGGGCGTTAGAGGAGGTGTCCGAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACgg                                         >  1:4234613/1‑59 (MQ=255)                                         aGGGCGTTAGAGGAGGTGTCCGAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAgaagaa                                >  1:1320443/1‑68 (MQ=255)                                          gggCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTg                            <  1:260290/71‑1 (MQ=255)                                          gggCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTg                            <  1:3965777/71‑1 (MQ=255)                                          gggCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCACCAGCCAGCCGAGGAACGGCGagaaga                                 <  1:4815674/66‑1 (MQ=255)                                            gCGTTAGAGGAGGTGTCCGAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGaa                          >  1:4260842/1‑71 (MQ=255)                                            gCGTTAGAGGAGGTGTCCGAGCCGCTAAGCAATACGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGaa                          >  1:3230097/1‑71 (MQ=255)                                               ttAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGagaag                                  <  1:5207988/60‑1 (MQ=255)                                               ttAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAAt                         <  1:5457942/69‑1 (MQ=255)                                               ttAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAAt                         <  1:3331589/69‑1 (MQ=255)                                               ttAGAGGAGGTGTCCGAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGaacat                       <  1:4839846/71‑4 (MQ=255)                                                 agagGAGGTGTCCGAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACggt                                        >  1:4210752/1‑51 (MQ=19)                                                 aGATGAGGTATCCGACCCGGTCAGGAATACCCCcagccagc                                                   >  1:4126596/1‑41 (MQ=255)                                                 aGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCAt                     >  1:3162993/1‑71 (MQ=255)                                                    tGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGa                   <  1:1856228/70‑1 (MQ=255)                                                     gAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGAcc                 >  1:2169980/1‑71 (MQ=255)                                                      aGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGt                             <  1:3951219/58‑1 (MQ=255)                                                       ggTGTCCGAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGagaa                                   >  1:5360147/1‑51 (MQ=19)                                                           tCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGTGagaag                                  >  1:3513565/1‑48 (MQ=255)                                                           tCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGagaag                                  >  1:140899/1‑48 (MQ=255)                                                             cGAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGaac                         >  1:1739630/1‑54 (MQ=255)                                                             cGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTgcg         >  1:4110773/1‑71 (MQ=255)                                                              gACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTgcgc        <  1:4141274/71‑1 (MQ=255)                                                              gACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTgcgc        <  1:2298358/71‑1 (MQ=255)                                                              gACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTgcgc        <  1:2511757/71‑1 (MQ=255)                                                               aCCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGcc       <  1:3007749/71‑1 (MQ=255)                                                                cccGGTCAGGAATACCCCCAGCCAGACGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCa      <  1:4808242/71‑1 (MQ=255)                                                                 ccGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAg     >  1:977483/1‑71 (MQ=255)                                                                    gTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATg                        <  1:274018/49‑1 (MQ=255)                                                                    gTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCa      <  1:2223517/67‑1 (MQ=255)                                                                    gTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCa      <  1:2022168/67‑1 (MQ=255)                                                                    gTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGc  <  1:813294/71‑1 (MQ=255)                                                                      cAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGt    >  1:5265785/1‑67 (MQ=255)                                                                      cAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGt    >  1:2502727/1‑67 (MQ=255)                                                                      cAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGt    >  1:4962244/1‑67 (MQ=255)                                                                      |                                                                     GCCAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGC  >  Exported/2562640‑2562777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑