BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	2,562,790	T→G	8.1%	S429S (TCA→TCC) ‡	lldP ←	L‑lactate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	2,562,790	0	T	G	8.1%	250.7 / 15.0	99	S429S (TCA→TCC) ‡	lldP	L‑lactate permease
Reads supporting (aligned to +/- strand): ref base T (47/44); new base G (3/5); total (50/49)
Fisher's exact test for biased strand distribution p-value = 4.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 117 total aligned reads displayed.

CCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGA  >  Exported/2562720‑2562859

ccagccagCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGtt                                                                       <  1:5148250/71‑1 (MQ=255)

ccagccagCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGtt                                                                       <  1:2558007/71‑1 (MQ=255)

 cagccagcCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGtt                                                                       >  1:5220691/1‑70 (MQ=255)

 cagccagcCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGtt                                                                       >  1:4552243/1‑70 (MQ=255)

 cagccagcCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGtt                                                                       >  1:4914321/1‑70 (MQ=255)

   gccagccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTtgat                                                                    <  1:3079144/71‑1 (MQ=255)

    ccagccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGtt                                                                       <  1:3007529/67‑1 (MQ=255)

    ccagccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTtgat                                                                    >  1:3048281/1‑70 (MQ=255)

    ccagccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTtgat                                                                    >  1:5356351/1‑70 (MQ=255)

     cagccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTtgatga                                                                  <  1:3350138/71‑1 (MQ=255)

     cagccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTtgatg                                                                   <  1:5118957/70‑1 (MQ=255)

     cagccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTtgatg                                                                   <  1:1558803/70‑1 (MQ=255)

       gccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTtgatga                                                                  >  1:2091311/1‑69 (MQ=255)

         cGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGt                                                              >  1:1914376/1‑71 (MQ=255)

          gAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTc                                                             >  1:4456498/1‑71 (MQ=255)

             gAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCgg                                                          <  1:5551658/71‑1 (MQ=255)

               aCGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGaa                                                        <  1:1619930/71‑1 (MQ=255)

               aCGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGaa                                                        >  1:3912729/1‑71 (MQ=255)

                    gagaAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGtt                                                   >  1:5157284/1‑71 (MQ=255)

                     agaagaAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTc                                                  <  1:3103375/71‑1 (MQ=255)

                       aagaagGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTcc                                                            <  1:618363/59‑1 (MQ=255)

                       aagaagGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGa                                                <  1:1586393/71‑1 (MQ=255)

                        agaagGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCg                                                 >  1:4599888/1‑69 (MQ=255)

                        agaagGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCg                                                 >  1:5578002/1‑69 (MQ=255)

                        agaagGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGa                                                >  1:4212015/1‑70 (MQ=255)

                         gaagGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGaaa                                              >  1:3084253/1‑71 (MQ=255)

                           agGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAAt                                             <  1:5508246/70‑1 (MQ=255)

                                 aTGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCg                                      <  1:1409973/71‑1 (MQ=255)

                                 aTGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCg                                      <  1:5415613/71‑1 (MQ=255)

                                 aTGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCg                                      <  1:3844628/71‑1 (MQ=255)

                                 aTGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCg                                      <  1:5047878/71‑1 (MQ=255)

                                 aTGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCg                                      <  1:2842602/71‑1 (MQ=255)

                                 aTGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCg                                      <  1:2822310/71‑1 (MQ=255)

                                   gCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATaa                                           <  1:2481695/64‑1 (MQ=255)

                                    cATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAAt                                   >  1:5169307/1‑71 (MQ=255)

                                    cATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAAt                                   >  1:1106407/1‑71 (MQ=255)

                                    cATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAAt                                   >  1:3893123/1‑71 (MQ=255)

                                    cATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAAt                                   >  1:106148/1‑71 (MQ=255)

                                    cATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCAAAATAAAGGCGAAt                                   >  1:638628/1‑71 (MQ=255)

                                        aCCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGaa                                               >  1:593341/1‑55 (MQ=255)

                                        aCCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGaa                                               >  1:5344643/1‑55 (MQ=255)

                                        aCCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAg                                         >  1:3647968/1‑61 (MQ=255)

                                        aCCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGc                                 >  1:4808018/1‑69 (MQ=255)

                                         ccGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGc                                       >  1:5466577/1‑62 (MQ=255)

                                         ccGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAg                              >  1:1020275/1‑71 (MQ=255)

                                         ccGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAg                              <  1:4606298/71‑1 (MQ=255)

                                          cGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATa                                            <  1:5176153/56‑1 (MQ=255)

                                          cGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATa                                            <  1:4622033/56‑1 (MQ=255)

                                             tgtgCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCg                                                 <  1:1141503/48‑1 (MQ=255)

                                               tgCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGa                                                <  1:1259051/47‑1 (MQ=255)

                                               tgCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATaaa                                          <  1:4947671/53‑1 (MQ=255)

                                                 cgcCAGTGCCAGCGCCAGTGTTGATGCCAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATAc                      >  1:4262622/1‑71 (MQ=255)

                                                 cgcCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGaaa                                              >  1:2550696/1‑47 (MQ=255)

                                                 cgcCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGaaa                                              >  1:4698748/1‑47 (MQ=255)

                                                 cgcCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGaaa                                              >  1:1418383/1‑47 (MQ=255)

                                                 cgcCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGaaa                                              >  1:1587077/1‑47 (MQ=255)

                                                 cgcCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAAt                                             <  1:5028110/48‑1 (MQ=255)

                                                 cgcCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATAc                      >  1:5556386/1‑71 (MQ=255)

                                                   ccAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATAcc                     >  1:3398620/1‑70 (MQ=255)

                                                   ccAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATAcc                     >  1:4946521/1‑70 (MQ=255)

                                                   ccAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCg                    <  1:4700014/71‑1 (MQ=255)

                                                    cAGTACCAGCGCCAGCGTGGTGGACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCa                         <  1:2633657/65‑1 (MQ=255)

                                                    cAGTACCAGCGCCAGCGTGGTGGACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGccaa                   <  1:4206624/71‑3 (MQ=255)

                                                    cAGTACCAGCGCCAGCGTGGTGGACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGccaa                   <  1:5280274/71‑3 (MQ=255)

                                                     aGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGAt                  >  1:239227/1‑71 (MQ=255)

                                                      gTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATg                 <  1:1936672/71‑1 (MQ=255)

                                                       tGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATa                                            <  1:1021423/43‑1 (MQ=255)

                                                       tGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATa                                            <  1:3836005/43‑1 (MQ=255)

                                                       tGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATa                                            <  1:4429701/43‑1 (MQ=255)

                                                       tGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAgg                                        <  1:3974195/47‑1 (MQ=255)

                                                       tGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAgg                                        <  1:4159643/47‑1 (MQ=255)

                                                         ccagcgccagcgTGGTGGACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGa               >  1:769714/1‑70 (MQ=255)

                                                         ccagcgccagcgTGGTGGACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGa               >  1:118170/1‑70 (MQ=255)

                                                         ccagcgccagcgTGGTGGACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGa               <  1:408708/70‑1 (MQ=255)

                                                         ccAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATa                       >  1:1750329/1‑62 (MQ=255)

                                                         ccAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAg              >  1:1110052/1‑71 (MQ=255)

                                                         ccAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAg              <  1:3234846/71‑1 (MQ=255)

                                                         ccAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAg              >  1:5545325/1‑71 (MQ=255)

                                                          cAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAg              >  1:5599599/1‑70 (MQ=255)

                                                          cAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAg              >  1:2693699/1‑70 (MQ=255)

                                                          cAGCGCCAGCGTGGTGGACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGa               <  1:2241158/69‑1 (MQ=255)

                                                          cAGCGACAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATCCCGATGGAg              >  1:2008963/1‑70 (MQ=255)

                                                           aGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATa                                            <  1:1635298/39‑1 (MQ=255)

                                                             cgcCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAg              >  1:4506506/1‑67 (MQ=255)

                                                              gcCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATAc                      <  1:4140878/58‑1 (MQ=255)

                                                               ccAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTa            <  1:5275838/67‑1 (MQ=255)

                                                                 aGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCa                         >  1:3025819/1‑52 (MQ=255)

                                                                 aGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCa                         >  1:1596421/1‑52 (MQ=255)

                                                                 aGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATggg      <  1:3210737/71‑1 (MQ=255)

                                                                  gtgtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGaa                                    >  1:2072745/1‑40 (MQ=255)

                                                                  gtgtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGaa                                    >  1:1405628/1‑40 (MQ=255)

                                                                  gtgtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGaa                                    >  1:5662538/1‑40 (MQ=255)

                                                                  gtgtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGa                                     <  1:2713171/39‑1 (MQ=255)

                                                                  gtgtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATggg      >  1:248751/1‑70 (MQ=255)

                                                                  gtgtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATggg      >  1:4043806/1‑70 (MQ=255)

                                                                   tgtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGAt                  <  1:464769/57‑1 (MQ=255)

                                                                   tgtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCa    <  1:1048842/71‑1 (MQ=255)

                                                                    gtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCgtt                  <  1:33239/56‑3 (MQ=255)

                                                                     ttGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATgg                >  1:4503950/1‑57 (MQ=255)

                                                                      tgatgaCAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCaga  <  1:2691275/70‑1 (MQ=255)

CCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGA  >  Exported/2562720‑2562859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`