BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	2,562,817	A→G	6.2%	F420F (TTT→TTC)	lldP ←	L‑lactate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	2,562,817	0	A	G	6.2%	384.2 / 10.1	134	F420F (TTT→TTC)	lldP	L‑lactate permease
Reads supporting (aligned to +/- strand): ref base A (69/55); new base G (3/5); total (72/62)
Fisher's exact test for biased strand distribution p-value = 4.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.79e-01

Only 100 of 142 total aligned reads displayed.

ATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAGCGTGCTGCCGA  >  Exported/2562753‑2562884

aTGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCg                                                               <  1:2822310/71‑1 (MQ=255)

aTGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCg                                                               <  1:5415613/71‑1 (MQ=255)

aTGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCg                                                               <  1:2842602/71‑1 (MQ=255)

aTGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCg                                                               <  1:1409973/71‑1 (MQ=255)

aTGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCg                                                               <  1:3844628/71‑1 (MQ=255)

aTGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCg                                                               <  1:4212770/71‑1 (MQ=255)

aTGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCg                                                               <  1:2597269/71‑1 (MQ=255)

aTGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCg                                                               <  1:835765/71‑1 (MQ=255)

   cATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAAt                                                            >  1:5169307/1‑71 (MQ=255)

   cATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAAt                                                            >  1:1106407/1‑71 (MQ=255)

   cATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAAt                                                            >  1:106148/1‑71 (MQ=255)

   cATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCAAAATAAAGGCGAAt                                                            >  1:638628/1‑71 (MQ=255)

       aCCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAg                                                                  >  1:3647968/1‑61 (MQ=255)

       aCCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGc                                                          >  1:4808018/1‑69 (MQ=255)

        ccGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAg                                                       <  1:4606298/71‑1 (MQ=255)

        ccGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAg                                                       >  1:1020275/1‑71 (MQ=255)

              tgCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATaaa                                                                   <  1:4947671/53‑1 (MQ=255)

                cgcCAGTGCCAGCGCCAGTGTTGATGCCAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATAc                                               >  1:4262622/1‑71 (MQ=255)

                  ccAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATAcc                                              >  1:3398620/1‑70 (MQ=255)

                  ccAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATAcc                                              >  1:4946521/1‑70 (MQ=255)

                  ccAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCg                                             <  1:4700014/71‑1 (MQ=255)

                   cAGTACCAGCGCCAGCGTGGTGGACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGccaa                                            <  1:5280274/71‑3 (MQ=255)

                    aGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGAt                                           >  1:239227/1‑71 (MQ=255)

                    aGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGAt                                           >  1:2159907/1‑71 (MQ=255)

                     gTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATg                                          <  1:1936672/71‑1 (MQ=255)

                      tGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATa                                                                     <  1:1021423/43‑1 (MQ=255)

                      tGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAgg                                                                 <  1:4159643/47‑1 (MQ=255)

                      tGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAgg                                                                 <  1:3974195/47‑1 (MQ=255)

                      tGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATg                                          >  1:5103044/1‑70 (MQ=255)

                      tGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAAT‑AAGGCGAATGCCAGCACCATACCGATGGa                                        <  1:1114507/71‑1 (MQ=255)

                        ccagcgccagcgTGGTGGACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGa                                        >  1:769714/1‑70 (MQ=255)

                        ccagcgccagcgTGGTGGACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGa                                        <  1:408708/70‑1 (MQ=255)

                        ccagcgccagcgTGGTGGACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGa                                        >  1:118170/1‑70 (MQ=255)

                        ccAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATa                                                >  1:1750329/1‑62 (MQ=255)

                        ccAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAg                                       >  1:5545325/1‑71 (MQ=255)

                         cAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAg                                       >  1:2693699/1‑70 (MQ=255)

                         cAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAg                                       >  1:5599599/1‑70 (MQ=255)

                          aGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATa                                                                     <  1:1635298/39‑1 (MQ=255)

                            cgcCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAg                                       >  1:4506506/1‑67 (MQ=255)

                             gcCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGa                                   >  1:4009300/1‑70 (MQ=255)

                              ccAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTa                                     <  1:5275838/67‑1 (MQ=255)

                                aGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCa                                                  >  1:3025819/1‑52 (MQ=255)

                                aGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATggg                               <  1:3210737/71‑1 (MQ=255)

                                 gtgtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGaa                                                             >  1:2072745/1‑40 (MQ=255)

                                 gtgtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGaa                                                             >  1:1405628/1‑40 (MQ=255)

                                 gtgtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGaa                                                             >  1:5662538/1‑40 (MQ=255)

                                 gtgtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATggg                               >  1:248751/1‑70 (MQ=255)

                                  tgtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGAt                                           <  1:464769/57‑1 (MQ=255)

                                  tgtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCa                             <  1:1048842/71‑1 (MQ=255)

                                  tgtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCa                             <  1:5484630/71‑1 (MQ=255)

                                   gtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCgtt                                           <  1:33239/56‑3 (MQ=255)

                                     tgatgaCAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCaga                           <  1:2691275/70‑1 (MQ=255)

                                      gatgaCAGTCCGGAATAGTTCGAAATAAAGGCGaa                                                             >  1:4512518/1‑35 (MQ=255)

                                      gatgaCAGTCCGGAATAGTTCGAAATAAAGGCGaa                                                             >  1:4021223/1‑35 (MQ=255)

                                      gatgaCAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCagag                          <  1:892243/70‑1 (MQ=255)

                                      gatgaCAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGc                         >  1:5530277/1‑71 (MQ=255)

                                      gatgaCAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGc                         >  1:1380088/1‑71 (MQ=255)

                                      gatgaCAGT‑CGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGc                         >  1:3243766/1‑70 (MQ=255)

                                           cAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCag                            >  1:1691005/1‑63 (MQ=255)

                                             gTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGa                                   >  1:1311283/1‑54 (MQ=255)

                                             gTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGa                                   >  1:1273389/1‑54 (MQ=255)

                                              tCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAg                                    <  1:1224405/52‑1 (MQ=255)

                                              tCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCATTTCtt                 >  1:5191361/1‑71 (MQ=255)

                                                cGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCagag                          >  1:895726/1‑60 (MQ=255)

                                                cGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAg                      >  1:4927924/1‑64 (MQ=255)

                                                cGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCttt                >  1:1495555/1‑70 (MQ=255)

                                                cGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCttt                >  1:4434644/1‑70 (MQ=255)

                                                cGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCttt                >  1:5252222/1‑70 (MQ=255)

                                                cGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCttt                >  1:3395007/1‑70 (MQ=255)

                                                cGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCttt                <  1:2064984/70‑1 (MQ=255)

                                                cGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGAT‑‑GCAGAGCCAGTTCTTTCAg             >  1:3351507/1‑71 (MQ=255)

                                                 ggAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGt                                      <  1:3769596/47‑1 (MQ=255)

                                                  gAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAg             <  1:4092279/71‑1 (MQ=255)

                                                  gAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAg             <  1:920468/71‑1 (MQ=255)

                                                   aaTAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAGc            >  1:818935/1‑71 (MQ=255)

                                                   aaTAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAGc            >  1:98151/1‑71 (MQ=255)

                                                   aaTAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAGc            >  1:5010301/1‑71 (MQ=255)

                                                    aTAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGa                                            >  1:3855829/1‑38 (MQ=255)

                                                    aTAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAGCg           >  1:4474416/1‑71 (MQ=255)

                                                    aTAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAGCg           >  1:2293695/1‑71 (MQ=255)

                                                     tAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGa                                            <  1:4163940/37‑1 (MQ=255)

                                                     tAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGAt                                           <  1:5232841/38‑1 (MQ=255)

                                                     tAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGAt                                           <  1:2100207/38‑1 (MQ=255)

                                                     tAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGAt                                           <  1:3255176/38‑1 (MQ=255)

                                                     tAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATggg                               >  1:735929/1‑50 (MQ=255)

                                                     tAGTTCGAAATAAACGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAGc            <  1:5394239/69‑1 (MQ=255)

                                                      aGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCa                             <  1:3090354/51‑1 (MQ=255)

                                                      aGTTCGAAAT‑AAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCa                             <  1:1365776/50‑1 (MQ=255)

                                                       gTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAGCGtgc        >  1:3021505/1‑71 (MQ=255)

                                                        ttCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAGCGtgct       <  1:2344279/71‑1 (MQ=255)

                                                        ttCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAGCGtgct       <  1:1887200/71‑1 (MQ=255)

                                                        ttCGAAATAAAGGCGAATGCCAGCACCATACCGAAGGAGTAGATGGGCAGAGCCAGtt                    <  1:1658827/58‑1 (MQ=255)

                                                          cGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTa                                     <  1:2279147/39‑1 (MQ=255)

                                                          cGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCa                       >  1:4261842/1‑53 (MQ=255)

                                                          cGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAGCGtg         >  1:2850442/1‑67 (MQ=255)

                                                           gAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAGCGTGCTGcc    <  1:2541147/71‑1 (MQ=255)

                                                            aaaTAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGt                     <  1:2167776/53‑1 (MQ=255)

                                                            aaaTAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGt                     <  1:3644682/53‑1 (MQ=255)

                                                             aaTAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAGCGTGCTGCCGa  <  1:3264120/71‑1 (MQ=255)

                                                             aaTAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAGCGTGCTGCCGa  <  1:1685378/71‑1 (MQ=255)

ATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAGCGTGCTGCCGA  >  Exported/2562753‑2562884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`