Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,569,763 G→T 7.5% L11L (CTG→CTT yibI → predicted inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,569,7630GT 7.5% 509.9 / 15.2 158L11L (CTG→CTTyibIpredicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (74/72);  new base T (9/3);  total (83/75)
Fisher's exact test for biased strand distribution p-value = 1.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.17e-01

Only 100 of 162 total aligned reads displayed.

CCAAAAACAACACTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAA  >  Exported/2569693‑2569831
                                                                      |                                                                    
ccAAAAACAACACTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTg                                                                      <  1:4756782/71‑1 (MQ=255)
 cAAAAACAACACTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGa                                                                     >  1:683986/1‑71 (MQ=255)
  aaaaaCAACACTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGAt                                                                    >  1:3321649/1‑71 (MQ=255)
    aaaCAACACTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCt                                                                  >  1:4832575/1‑71 (MQ=255)
    aaaCAACACTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCt                                                                  >  1:5094323/1‑71 (MQ=255)
     aacaacACTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCtt                                                                 >  1:842408/1‑71 (MQ=255)
     aacaacACTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCtt                                                                 >  1:5352950/1‑71 (MQ=255)
     aacaacACTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCtt                                                                 >  1:3537512/1‑71 (MQ=255)
     aacaacACTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCt                                                                  >  1:4375772/1‑70 (MQ=255)
       caacaCTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGAt                                                                    >  1:3605055/1‑66 (MQ=255)
       caaaaCTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGa                                                                     >  1:791402/1‑65 (MQ=255)
        aacaCTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTGGCGTTGGGAGTGCTGATCTTCGt                                                              >  1:4646370/1‑71 (MQ=255)
        aacaCTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCg                                                               >  1:3557853/1‑70 (MQ=255)
          cacTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTg                                                                      <  1:2813762/61‑1 (MQ=255)
              gATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCt                                                        >  1:1311394/1‑71 (MQ=255)
               aTAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCt                                                        <  1:3256525/70‑1 (MQ=255)
                tAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTg                                                       >  1:2546584/1‑70 (MQ=255)
                 aaaCGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTgg                                                      <  1:4648967/70‑1 (MQ=255)
                 aaaCGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGt                                                     >  1:5505269/1‑71 (MQ=255)
                 aaaCGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGt                                                     >  1:106797/1‑71 (MQ=255)
                  aaCGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGt                                                     >  1:5635951/1‑70 (MQ=255)
                   aCGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGa                                                   <  1:4766492/71‑1 (MQ=255)
                      tGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCtt                                                           <  1:2832469/60‑1 (MQ=255)
                        aaTCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCttt                                                          >  1:1265371/1‑59 (MQ=255)
                        aaTCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCttt                                                          >  1:3039435/1‑59 (MQ=255)
                        aaTCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTc                                                         >  1:1949962/1‑60 (MQ=255)
                        aaTCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAttttt                                              <  1:945975/71‑1 (MQ=255)
                        aaTCCGAGGCAGATATGTTTCTAAACTATATCGCGTTGGGAGTGCTGATCTTCGt                                                              >  1:1106759/1‑55 (MQ=255)
                         aTCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAtttttt                                             <  1:5032271/71‑1 (MQ=255)
                          tttgAGGGAAATATGTTCCTGGACTATTTTGCACTGGGAGTGCTTATTTTTGTATTTCTgg                                                      >  1:3678299/4‑61 (MQ=255)
                          tCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTc                                                         <  1:4213653/58‑1 (MQ=255)
                            cGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATg                                          >  1:2014749/1‑71 (MQ=255)
                            cGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATg                                          >  1:313801/1‑71 (MQ=255)
                              aGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATgg                                         >  1:3293467/1‑70 (MQ=255)
                              aGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGa                                        <  1:1007464/71‑1 (MQ=255)
                               ggCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGaa                                       <  1:5426406/71‑1 (MQ=255)
                               ggCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGaa                                       <  1:4966574/71‑1 (MQ=255)
                                 cAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATgg                                         <  1:2975868/67‑1 (MQ=255)
                                   gATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAatcat                                   >  1:3468664/1‑71 (MQ=255)
                                    atatGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAatcatc                                  <  1:4388811/71‑1 (MQ=255)
                                    atatGTTCCTGGACTATTTTGCACTGGGAGTGCTTATTTTTGTATTTCTGGt                                                     >  1:5566425/1‑52 (MQ=255)
                                     tatGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAttttt                                              <  1:5279947/58‑1 (MQ=255)
                                      atGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGt                                                     >  1:162784/1‑50 (MQ=255)
                                      atGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGt                                                     >  1:334821/1‑50 (MQ=255)
                                      atGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGt                                                     >  1:4197234/1‑50 (MQ=255)
                                      atGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATcgc                                >  1:2824968/1‑71 (MQ=255)
                                       tGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCt                                                                  <  1:820924/36‑1 (MQ=255)
                                         tttCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAtttttt                                             <  1:260697/55‑1 (MQ=255)
                                          ttCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa                              >  1:1112452/1‑69 (MQ=255)
                                          ttCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa                              >  1:724577/1‑69 (MQ=255)
                                           tCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTc                                                         >  1:1649948/1‑41 (MQ=255)
                                           tCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATa                            <  1:5518815/70‑1 (MQ=255)
                                            cTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACa                          >  1:5613240/1‑71 (MQ=255)
                                             tAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTAt                                           <  1:3014191/53‑1 (MQ=255)
                                             tAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACa                          >  1:1577962/1‑70 (MQ=255)
                                             tAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACAt                         >  1:1824320/1‑71 (MQ=255)
                                               aaCTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGTTGATTTTTTATGGAATCATCGCGATACATGa                       <  1:4099609/71‑1 (MQ=255)
                                               aaCTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTAt                                           <  1:5147232/51‑1 (MQ=255)
                                               aaCTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACAt                         <  1:3771123/69‑1 (MQ=255)
                                               aaCTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACAt                         <  1:1428015/69‑1 (MQ=255)
                                                  tATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAtttt                                               <  1:4214106/44‑1 (MQ=255)
                                                  tATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAtttt                                               <  1:5078520/44‑1 (MQ=255)
                                                  tATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATgg                                         <  1:1665762/50‑1 (MQ=255)
                                                  tATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATgg                                         <  1:4518434/50‑1 (MQ=255)
                                                  tATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATgg                                         <  1:4727819/50‑1 (MQ=255)
                                                    tttCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTa                                            >  1:525027/1‑45 (MQ=255)
                                                    tttCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTa                                            >  1:4201287/1‑45 (MQ=255)
                                                    tttCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATg                                          <  1:2016093/47‑1 (MQ=255)
                                                      tCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATcgc                                >  1:5028595/1‑55 (MQ=255)
                                                      tCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCt                >  1:320574/1‑71 (MQ=255)
                                                       cgcgTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATgg                                         <  1:2830359/45‑1 (MQ=255)
                                                       cgcgTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCt                >  1:2503064/1‑70 (MQ=255)
                                                        gcgTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCtt               <  1:1162472/70‑1 (MQ=255)
                                                          gTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATc            >  1:1804155/1‑71 (MQ=255)
                                                           ttGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTAt                                           <  1:2308373/39‑1 (MQ=255)
                                                           ttGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt           <  1:2252363/71‑1 (MQ=255)
                                                           ctGGGAGTGCTTATTTTTGTATTTCTGGTGATCTTCTATGGGatcat                                   <  1:2136477/46‑1 (MQ=255)
                                                           ctGGGAGTGCTTATTTTTGTATTTCTGGTGATCTTCTATGGGatcat                                   <  1:3523727/46‑1 (MQ=255)
                                                            tGGGAGTGCTGATCTTCGTCTTTCTGGTGAttttt                                              <  1:3678832/35‑1 (MQ=255)
                                                            tGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATAc                           >  1:4174044/1‑54 (MQ=255)
                                                            tGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGa                       >  1:58755/1‑58 (MQ=255)
                                                            tGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt           >  1:644274/1‑70 (MQ=255)
                                                             gggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTa                                            >  1:259420/1‑36 (MQ=255)
                                                             gggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTa                                            >  1:1899228/1‑36 (MQ=255)
                                                             gggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTg          <  1:552288/70‑1 (MQ=255)
                                                              ggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGa         <  1:141501/70‑1 (MQ=255)
                                                              ggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGa         <  1:222583/70‑1 (MQ=255)
                                                                 gTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATcgc                                <  1:726336/44‑1 (MQ=255)
                                                                 gTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa                              >  1:696116/1‑46 (MQ=255)
                                                                 gTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGc     <  1:812098/71‑1 (MQ=255)
                                                                  tGCTTATTTTTGTATTTCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGc     >  1:4424675/1‑70 (MQ=255)
                                                                  tGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGAt                             <  1:3392378/46‑1 (MQ=255)
                                                                  tGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGAt                             <  1:2991637/46‑1 (MQ=255)
                                                                   gCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt           <  1:1895950/63‑1 (MQ=255)
                                                                   gCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt           <  1:2916058/63‑1 (MQ=255)
                                                                    cTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCtt               <  1:4868967/58‑1 (MQ=255)
                                                                    cTGATCTTCGTCTCTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCa   >  1:5255712/1‑70 (MQ=255)
                                                                     tGATCTTCGTCTTTCTGGTGATTTTTTATGGAatca                                    >  1:5554549/1‑36 (MQ=255)
                                                                     tGATCTTCGTCTTTCTGGTGATTTTTTATGGAatca                                    >  1:2386579/1‑36 (MQ=255)
                                                                     tGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGc     <  1:777398/67‑1 (MQ=255)
                                                                      |                                                                    
CCAAAAACAACACTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAA  >  Exported/2569693‑2569831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: