Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,569,766 C→T 7.5% I12I (ATC→ATT yibI → predicted inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,569,7660CT 7.5% 471.1 / 17.9 160I12I (ATC→ATTyibIpredicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (73/75);  new base T (9/3);  total (82/78)
Fisher's exact test for biased strand distribution p-value = 1.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01

Only 100 of 163 total aligned reads displayed.

AAACAACACTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAG  >  Exported/2569697‑2569835
                                                                     |                                                                     
aaaCAACACTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCt                                                                      <  1:59929/71‑1 (MQ=255)
aaaCAACACTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCt                                                                      >  1:5094323/1‑71 (MQ=255)
 aacaacACTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCtt                                                                     >  1:842408/1‑71 (MQ=255)
 aacaacACTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCtt                                                                     >  1:3537512/1‑71 (MQ=255)
 aacaacACTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCtt                                                                     >  1:5352950/1‑71 (MQ=255)
    aacaCTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTGGCGTTGGGAGTGCTGATCTTCGt                                                                  >  1:4646370/1‑71 (MQ=255)
    aacaCTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCg                                                                   >  1:3557853/1‑70 (MQ=255)
    aacaCTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGt                                                                  >  1:1656650/1‑71 (MQ=255)
      cacTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCt                                                                <  1:2042810/71‑1 (MQ=255)
          gATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCt                                                            >  1:1311394/1‑71 (MQ=255)
           aTAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCt                                                            <  1:3256525/70‑1 (MQ=255)
             aaaCGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTgg                                                          <  1:4648967/70‑1 (MQ=255)
             aaaCGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGt                                                         >  1:2818754/1‑71 (MQ=255)
             aaaCGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGt                                                         >  1:106797/1‑71 (MQ=255)
              aaCGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGt                                                         >  1:5635951/1‑70 (MQ=255)
               aCGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGa                                                       <  1:4209238/71‑1 (MQ=255)
                    aaTCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCttt                                                              >  1:1265371/1‑59 (MQ=255)
                    aaTCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAttttt                                                  <  1:945975/71‑1 (MQ=255)
                    aaTCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAttttt                                                  <  1:5611426/71‑1 (MQ=255)
                    aaTCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAttttt                                                  >  1:3293845/1‑71 (MQ=255)
                    aaTCCGAGGCAGATATGTTTCTAAACTATATCGCGTTGGGAGTGCTGATCTTCGt                                                                  >  1:1106759/1‑55 (MQ=255)
                     aTCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAtttttt                                                 <  1:3006193/71‑1 (MQ=255)
                     aTCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAtttttt                                                 <  1:5032271/71‑1 (MQ=255)
                     aTCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAttttt                                                  >  1:687717/1‑70 (MQ=255)
                      tttgAGGGAAATATGTTCCTGGACTATTTTGCACTGGGAGTGCTTATTTTTGTATTTCTgg                                                          >  1:3678299/4‑61 (MQ=255)
                      tCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTc                                                             <  1:4213653/58‑1 (MQ=255)
                      tCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTa                                                >  1:3368057/1‑71 (MQ=255)
                        cGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTg                                                           >  1:3068534/1‑58 (MQ=255)
                        cGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATg                                              >  1:2014749/1‑71 (MQ=255)
                        cGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATg                                              >  1:313801/1‑71 (MQ=255)
                          aGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGa                                            <  1:1007464/71‑1 (MQ=255)
                           ggCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGaa                                           <  1:4966574/71‑1 (MQ=255)
                           ggCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGaa                                           <  1:5426406/71‑1 (MQ=255)
                             cAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATgg                                             <  1:2975868/67‑1 (MQ=255)
                             cAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAatc                                         <  1:4377304/71‑1 (MQ=255)
                             cAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAatc                                         <  1:2532546/71‑1 (MQ=255)
                               aatatGTTCCTGGACTATTTTGCACTGGGAGTGCTTATTTTTGTATTTCTGGTGATCTTCTATGGGatcat                                       >  1:70260/2‑71 (MQ=255)
                                atatGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAatcatc                                      <  1:4388811/71‑1 (MQ=255)
                                atatGTTCCTGGACTATTTTGCACTGGGAGTGCTTATTTTTGTATTTCTGGt                                                         >  1:19087/1‑52 (MQ=255)
                                atatGTTCCTGGACTATTTTGCACTGGGAGTGCTTATTTTTGTATTTCTGGt                                                         >  1:5566425/1‑52 (MQ=255)
                                 tatGTTCCTGGACTATTTTGCACTGGGAGTGCTTATTTTTGTATTTCTGGTGAt                                                      >  1:886156/1‑54 (MQ=255)
                                  atGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTg                                                           >  1:26027/1‑48 (MQ=255)
                                  atGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGt                                                         >  1:4197234/1‑50 (MQ=255)
                                  atGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGt                                                         >  1:162784/1‑50 (MQ=255)
                                  atGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGt                                                         >  1:5109092/1‑50 (MQ=255)
                                  atGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGt                                                         >  1:3513330/1‑50 (MQ=255)
                                  atGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTa                                                >  1:3283458/1‑59 (MQ=255)
                                  atGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATcgc                                    >  1:2824968/1‑71 (MQ=255)
                                   tGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTa                                                >  1:417653/1‑58 (MQ=255)
                                   tGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATcgc                                    <  1:4112691/70‑1 (MQ=255)
                                    gTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa                                  >  1:1796788/1‑71 (MQ=255)
                                     tttCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAtttttt                                                 <  1:4360511/55‑1 (MQ=255)
                                      ttCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTa                                                >  1:1518927/1‑55 (MQ=255)
                                      ttCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa                                  >  1:1112452/1‑69 (MQ=255)
                                      ttCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa                                  >  1:724577/1‑69 (MQ=255)
                                       tCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTc                                                             >  1:3589946/1‑41 (MQ=255)
                                       tCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATg                                              <  1:5540174/56‑1 (MQ=255)
                                       tCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATa                                <  1:5518815/70‑1 (MQ=255)
                                         tAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTAt                                               <  1:3014191/53‑1 (MQ=255)
                                           aaCTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACAt                             <  1:1428015/69‑1 (MQ=255)
                                           aaCTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACAt                             <  1:3771123/69‑1 (MQ=255)
                                           aaCTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGa                           <  1:5066146/71‑1 (MQ=255)
                                              tATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATgg                                             <  1:4727819/50‑1 (MQ=255)
                                                 ttCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAatc                                         >  1:217316/1‑51 (MQ=255)
                                                  tCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCt                    >  1:320574/1‑71 (MQ=255)
                                                   cgcgTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATgg                                             <  1:4756952/45‑1 (MQ=255)
                                                   cgcgTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATgg                                             <  1:1957759/45‑1 (MQ=255)
                                                   cgcgTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATgg                                             <  1:2830359/45‑1 (MQ=255)
                                                    gcgTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCtt                   <  1:1162472/70‑1 (MQ=255)
                                                      gTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATc                >  1:1804155/1‑71 (MQ=255)
                                                       ttGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTAt                                               <  1:5277875/39‑1 (MQ=255)
                                                       ctGGGAGTGCTTATTTTTGTATTTCTGGTGATCTTCTATGGGatcat                                       <  1:3523727/46‑1 (MQ=255)
                                                        tGGGAGTGCTGATCTTCGTCTTTCTGGTGAttttt                                                  <  1:2437320/35‑1 (MQ=255)
                                                        tGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGa                           >  1:58755/1‑58 (MQ=255)
                                                         gggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTa                                                >  1:1899228/1‑36 (MQ=255)
                                                         gggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTa                                                >  1:259420/1‑36 (MQ=255)
                                                         gggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTg              <  1:552288/70‑1 (MQ=255)
                                                          ggAGTGCTTATTTTTGTATTTCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGAt            >  1:4515618/1‑71 (MQ=255)
                                                          ggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGa             <  1:5462382/70‑1 (MQ=255)
                                                          ggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGa             <  1:222583/70‑1 (MQ=255)
                                                             gTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa                                  >  1:2265264/1‑46 (MQ=255)
                                                             gTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGc         <  1:812098/71‑1 (MQ=255)
                                                              tGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGAt                                 <  1:3711333/46‑1 (MQ=255)
                                                              tGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGAt                                 <  1:1850571/46‑1 (MQ=255)
                                                              tGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGAt                                 <  1:3392378/46‑1 (MQ=255)
                                                              tGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt               <  1:4820180/64‑1 (MQ=255)
                                                               gCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGa                           <  1:2854505/51‑1 (MQ=255)
                                                               gCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt               <  1:2916058/63‑1 (MQ=255)
                                                                cTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCtt                   <  1:4868967/58‑1 (MQ=255)
                                                                cTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaa      <  1:502164/71‑1 (MQ=255)
                                                                cTGATCTTCGTCTCTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCa       >  1:5255712/1‑70 (MQ=255)
                                                                 tGATCTTCGTCTTTCTGGTGATTTTTTATGGAatca                                        >  1:2386579/1‑36 (MQ=255)
                                                                 tGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACa                         >  1:743848/1‑51 (MQ=255)
                                                                 tGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACa                         >  1:815750/1‑51 (MQ=255)
                                                                 tGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGc         <  1:777398/67‑1 (MQ=255)
                                                                 tGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGc         <  1:1624129/67‑1 (MQ=255)
                                                                   aTCTTCGTCTTTCTGGTGATTTTTTATGGAatcat                                       >  1:892416/1‑35 (MQ=255)
                                                                   aTCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaa     <  1:806317/69‑1 (MQ=255)
                                                                   aTCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTAGCTGATTGCCaaa     >  1:5178328/1‑69 (MQ=255)
                                                                     cTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGAt            >  1:1338825/1‑60 (MQ=255)
                                                                     |                                                                     
AAACAACACTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAG  >  Exported/2569697‑2569835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: