Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,569,769 C→T 7.6% F13F (TTC→TTT yibI → predicted inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,569,7690CT 7.6% 460.2 / 17.3 157F13F (TTC→TTTyibIpredicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (71/74);  new base T (9/3);  total (80/77)
Fisher's exact test for biased strand distribution p-value = 1.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

Only 100 of 163 total aligned reads displayed.

AACACTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGC  >  Exported/2569701‑2569838
                                                                    |                                                                     
aacaCTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTGGCGTTGGGAGTGCTGATCTTCGt                                                                     >  1:4646370/1‑71 (MQ=255)
aacaCTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCg                                                                      >  1:3557853/1‑70 (MQ=255)
      gATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCt                                                               >  1:1311394/1‑71 (MQ=255)
       aTAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCt                                                               <  1:3256525/70‑1 (MQ=255)
        tAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTgg                                                             >  1:3672462/1‑71 (MQ=255)
        tAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTg                                                              >  1:2546584/1‑70 (MQ=255)
         aaaCGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGt                                                            >  1:106797/1‑71 (MQ=255)
         aaaCGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGt                                                            >  1:5505269/1‑71 (MQ=255)
          aaCGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGt                                                            >  1:5635951/1‑70 (MQ=255)
           aCGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGa                                                          <  1:4209238/71‑1 (MQ=255)
              tGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCtt                                                                  <  1:2832469/60‑1 (MQ=255)
                aaTCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCttt                                                                 >  1:1265371/1‑59 (MQ=255)
                aaTCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTc                                                                >  1:1949962/1‑60 (MQ=255)
                aaTCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAttttt                                                     <  1:945975/71‑1 (MQ=255)
                aaTCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAttttt                                                     >  1:3293845/1‑71 (MQ=255)
                aaTCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAtttt                                                      <  1:3528104/70‑1 (MQ=255)
                aaTCCGAGGCAGATATGTTTCTAAACTATATCGCGTTGGGAGTGCTGATCTTCGt                                                                     >  1:1106759/1‑55 (MQ=255)
                 aTCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAtttttt                                                    <  1:3006193/71‑1 (MQ=255)
                 aTCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAtttttt                                                    <  1:5032271/71‑1 (MQ=255)
                 aTCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAttttt                                                     >  1:687717/1‑70 (MQ=255)
                  tttgAGGGAAATATGTTCCTGGACTATTTTGCACTGGGAGTGCTTATTTTTGTATTTCTgg                                                             >  1:3678299/4‑61 (MQ=255)
                  tCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTc                                                                <  1:4213653/58‑1 (MQ=255)
                  tCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTa                                                   >  1:3368057/1‑71 (MQ=255)
                    cGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTg                                                              >  1:3068534/1‑58 (MQ=255)
                      aGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATgg                                                >  1:3293467/1‑70 (MQ=255)
                      aGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGa                                               <  1:1007464/71‑1 (MQ=255)
                       ggCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGaa                                              <  1:5426406/71‑1 (MQ=255)
                       ggCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGaa                                              <  1:4966574/71‑1 (MQ=255)
                         cAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATgg                                                <  1:2975868/67‑1 (MQ=255)
                         cAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAatc                                            <  1:2532546/71‑1 (MQ=255)
                           gATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAatcat                                          >  1:3468664/1‑71 (MQ=255)
                            atatGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAatcatc                                         <  1:4388811/71‑1 (MQ=255)
                            atatGTTCCTGGACTATTTTGCACTGGGAGTGCTTATTTTTGTATTTCTGGt                                                            >  1:5566425/1‑52 (MQ=255)
                             tatGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAttttt                                                     <  1:5279947/58‑1 (MQ=255)
                             tatGTTCCTGGACTATTTTGCACTGGGAGTGCTTATTTTTGTATTTCTGGTGAt                                                         >  1:4655440/1‑54 (MQ=255)
                             tatGTTCCTGGACTATTTTGCACTGGGAGTGCTTATTTTTGTATTTCTGGTGAt                                                         >  1:886156/1‑54 (MQ=255)
                              atGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTg                                                              >  1:26027/1‑48 (MQ=255)
                              atGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGt                                                            >  1:334821/1‑50 (MQ=255)
                              atGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGt                                                            >  1:4197234/1‑50 (MQ=255)
                              atGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATcgc                                       >  1:2824968/1‑71 (MQ=255)
                               tGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATcgc                                       <  1:4112691/70‑1 (MQ=255)
                                gTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa                                     >  1:1796788/1‑71 (MQ=255)
                                 tttCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAtttttt                                                    <  1:4360511/55‑1 (MQ=255)
                                 tttCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAtttttt                                                    <  1:260697/55‑1 (MQ=255)
                                  ttCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa                                     >  1:724577/1‑69 (MQ=255)
                                  ttCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa                                     >  1:1112452/1‑69 (MQ=255)
                                   tCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTc                                                                >  1:1649948/1‑41 (MQ=255)
                                   tCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATg                                                 <  1:5540174/56‑1 (MQ=255)
                                   tCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATa                                   <  1:5518815/70‑1 (MQ=255)
                                    cTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACa                                 >  1:5613240/1‑71 (MQ=255)
                                     tAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTAt                                                  <  1:3014191/53‑1 (MQ=255)
                                     tAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACa                                 >  1:1577962/1‑70 (MQ=255)
                                     tAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACAt                                >  1:1824320/1‑71 (MQ=255)
                                       aaCTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTAt                                                  <  1:5147232/51‑1 (MQ=255)
                                       aaCTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACAt                                <  1:1428015/69‑1 (MQ=255)
                                       aaCTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACAt                                <  1:3771123/69‑1 (MQ=255)
                                          tATTTTGCACTGGGAGTGCTTATTTTTGTATTTCTGGTGAtcttc                                                     <  1:811979/45‑2 (MQ=255)
                                          tATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAtttt                                                      <  1:5078520/44‑1 (MQ=255)
                                          tATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATgg                                                <  1:1665762/50‑1 (MQ=255)
                                          tATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATgg                                                <  1:4518434/50‑1 (MQ=255)
                                          tATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATgg                                                <  1:4727819/50‑1 (MQ=255)
                                            tttCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTa                                                   >  1:525027/1‑45 (MQ=255)
                                            tttCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATg                                                 <  1:2016093/47‑1 (MQ=255)
                                             ttCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAatc                                            >  1:217316/1‑51 (MQ=255)
                                              tCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATcgc                                       >  1:5028595/1‑55 (MQ=255)
                                              tCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCt                       >  1:320574/1‑71 (MQ=255)
                                               cgcgTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATgg                                                <  1:4756952/45‑1 (MQ=255)
                                               cgcgTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATgg                                                <  1:2830359/45‑1 (MQ=255)
                                               cgcgTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATgg                                                <  1:1957759/45‑1 (MQ=255)
                                               cgcgTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCt                       >  1:2503064/1‑70 (MQ=255)
                                                gcgTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCtt                      <  1:1162472/70‑1 (MQ=255)
                                                   ttGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTAt                                                  <  1:5277875/39‑1 (MQ=255)
                                                   ttGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTAt                                                  <  1:2308373/39‑1 (MQ=255)
                                                   ttGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt                  <  1:2252363/71‑1 (MQ=255)
                                                   ctGGGAGTGCTTATTTTTGTATTTCTGGTGATCTTCTATGGGatcat                                          <  1:2136477/46‑1 (MQ=255)
                                                   ctGGGAGTGCTTATTTTTGTATTTCTGGTGATCTTCTATGGGatcat                                          <  1:3523727/46‑1 (MQ=255)
                                                    tGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATAc                                  >  1:4174044/1‑54 (MQ=255)
                                                    tGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGa                              >  1:58755/1‑58 (MQ=255)
                                                     gggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTa                                                   >  1:1899228/1‑36 (MQ=255)
                                                     gggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTg                 <  1:552288/70‑1 (MQ=255)
                                                      ggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGa                <  1:141501/70‑1 (MQ=255)
                                                       gAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAatca                                           >  1:3162746/1‑42 (MQ=255)
                                                         gTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATcgc                                       <  1:726336/44‑1 (MQ=255)
                                                         gTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa                                     >  1:696116/1‑46 (MQ=255)
                                                          tGCTTATTTTTGTATTTCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGc            >  1:4424675/1‑70 (MQ=255)
                                                          tGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGAt                                    <  1:3711333/46‑1 (MQ=255)
                                                          tGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt                  <  1:4820180/64‑1 (MQ=255)
                                                           gCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt                  <  1:2916058/63‑1 (MQ=255)
                                                           gCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt                  <  1:1895950/63‑1 (MQ=255)
                                                             tGATCTTCGTCTTTCTGGTGATTTTTTATGGAatca                                           >  1:2386579/1‑36 (MQ=255)
                                                             tGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACa                            >  1:815750/1‑51 (MQ=255)
                                                             tGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGc            <  1:777398/67‑1 (MQ=255)
                                                             tGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGc            <  1:1624129/67‑1 (MQ=255)
                                                               aTCTTCGTCTTTCTGGTGATTTTTTATGGAatcat                                          >  1:892416/1‑35 (MQ=255)
                                                               aTCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaa        <  1:806317/69‑1 (MQ=255)
                                                                tCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg     >  1:4984488/1‑71 (MQ=255)
                                                                 cTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGAt               >  1:1338825/1‑60 (MQ=255)
                                                                   tCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa                                     >  1:3704124/1‑36 (MQ=255)
                                                                    cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa                                     >  1:2987201/1‑35 (MQ=255)
                                                                    cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaaa      <  1:5167353/66‑1 (MQ=255)
                                                                    |                                                                     
AACACTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGC  >  Exported/2569701‑2569838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: