Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,569,772 C→A 6.8% V14V (GTC→GTA yibI → predicted inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,569,7720CA 6.8% 556.1 / 12.2 171V14V (GTC→GTAyibIpredicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (71/88);  new base A (9/3);  total (80/91)
Fisher's exact test for biased strand distribution p-value = 6.86e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.66e-01

Only 100 of 172 total aligned reads displayed.

CACTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAC  >  Exported/2569703‑2569841
                                                                     |                                                                     
cacTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCt                                                                      <  1:2042810/71‑1 (MQ=255)
    gATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCt                                                                  >  1:1311394/1‑71 (MQ=255)
     aTAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCt                                                                  <  1:3256525/70‑1 (MQ=255)
      tAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTgg                                                                >  1:3672462/1‑71 (MQ=255)
       aaaCGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTgg                                                                <  1:4648967/70‑1 (MQ=255)
       aaaCGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGt                                                               >  1:106797/1‑71 (MQ=255)
       aaaCGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGt                                                               >  1:5505269/1‑71 (MQ=255)
       aaaCGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGt                                                               >  1:2818754/1‑71 (MQ=255)
        aaCGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGt                                                               >  1:5635951/1‑70 (MQ=255)
         aCGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGa                                                             <  1:4209238/71‑1 (MQ=255)
            tGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCtt                                                                     <  1:2832469/60‑1 (MQ=255)
              aaTCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTc                                                                   >  1:1949962/1‑60 (MQ=255)
              aaTCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAttttt                                                        <  1:945975/71‑1 (MQ=255)
              aaTCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAttttt                                                        <  1:5611426/71‑1 (MQ=255)
              aaTCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAttttt                                                        >  1:3293845/1‑71 (MQ=255)
               aTCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAtttttt                                                       <  1:5032271/71‑1 (MQ=255)
                tCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTc                                                                   <  1:4213653/58‑1 (MQ=255)
                 ccGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTAt                                                     >  1:2937627/1‑71 (MQ=255)
                  cGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTg                                                                 >  1:3068534/1‑58 (MQ=255)
                  cGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATg                                                    >  1:2014749/1‑71 (MQ=255)
                    aGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATgg                                                   >  1:3293467/1‑70 (MQ=255)
                    aGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGa                                                  <  1:1007464/71‑1 (MQ=255)
                     ggCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGaa                                                 <  1:5426406/71‑1 (MQ=255)
                       cAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAatc                                               <  1:2532546/71‑1 (MQ=255)
                         gATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTc                                                                   <  1:2825236/49‑1 (MQ=255)
                          atatGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAatcatc                                            <  1:4388811/71‑1 (MQ=255)
                           tatGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAttttt                                                        <  1:5279947/58‑1 (MQ=255)
                            atGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTg                                                                 >  1:26027/1‑48 (MQ=255)
                            atGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGt                                                               >  1:162784/1‑50 (MQ=255)
                            atGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGt                                                               >  1:4197234/1‑50 (MQ=255)
                            atGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGt                                                               >  1:334821/1‑50 (MQ=255)
                            atGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGt                                                               >  1:5109092/1‑50 (MQ=255)
                            atGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGt                                                               >  1:3513330/1‑50 (MQ=255)
                             tGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATcgc                                          <  1:571171/70‑1 (MQ=255)
                              gTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa                                        >  1:1796788/1‑71 (MQ=255)
                               tttCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAtttttt                                                       <  1:4360511/55‑1 (MQ=255)
                                ttCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTa                                                      >  1:1518927/1‑55 (MQ=255)
                                ttCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa                                        >  1:724577/1‑69 (MQ=255)
                                ttCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa                                        >  1:1112452/1‑69 (MQ=255)
                                 tCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATg                                                    <  1:5540174/56‑1 (MQ=255)
                                   tAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTAt                                                     <  1:3014191/53‑1 (MQ=255)
                                   tAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACAt                                   >  1:1824320/1‑71 (MQ=255)
                                     gaCTATTTTGCACTGGGAGTGCTTATTTTTGTATTTCTGGTGATCTTCTATGGGat                                                >  1:5522801/2‑56 (MQ=255)
                                     aaCTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGTTGATTTTTTATGGAATCATCGCGATACATGa                                 <  1:4099609/71‑1 (MQ=255)
                                     aaCTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATg                                                    >  1:1265138/1‑52 (MQ=255)
                                     aaCTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACAt                                   <  1:1428015/69‑1 (MQ=255)
                                     aaCTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGa                                 <  1:5066146/71‑1 (MQ=255)
                                        tATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATgg                                                   <  1:4518434/50‑1 (MQ=255)
                                        tATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATgg                                                   <  1:1665762/50‑1 (MQ=255)
                                          tttCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTa                                                      >  1:525027/1‑45 (MQ=255)
                                           ttCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAatc                                               >  1:217316/1‑51 (MQ=255)
                                            tCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCt                          >  1:320574/1‑71 (MQ=255)
                                             cgcgTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATgg                                                   <  1:1957759/45‑1 (MQ=255)
                                             cgcgTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATgg                                                   <  1:4756952/45‑1 (MQ=255)
                                             cgcgTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCt                          >  1:2503064/1‑70 (MQ=255)
                                              gcgTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCtt                         <  1:1162472/70‑1 (MQ=255)
                                                 ttGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTAt                                                     <  1:2308373/39‑1 (MQ=255)
                                                 ttGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt                     <  1:2252363/71‑1 (MQ=255)
                                                 ctGGGAGTGCTTATTTTTGTATTTCTGGTGATCTTCTATGGGatcat                                             <  1:3523727/46‑1 (MQ=255)
                                                 ctGGGAGTGCTTATTTTTGTATTTCTGGTGATCTTCTATGGGatcat                                             <  1:2136477/46‑1 (MQ=255)
                                                  tGGGAGTGCTGATCTTCGTCTTTCTGGTGAttttt                                                        <  1:3678832/35‑1 (MQ=255)
                                                  tGGGAGTGCTGATCTTCGTCTTTCTGGTGAttttt                                                        <  1:2437320/35‑1 (MQ=255)
                                                  tGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATAc                                     >  1:4174044/1‑54 (MQ=255)
                                                  tGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt                     >  1:644274/1‑70 (MQ=255)
                                                   gggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTa                                                      >  1:1899228/1‑36 (MQ=255)
                                                   gggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTa                                                      >  1:259420/1‑36 (MQ=255)
                                                    ggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGa                   <  1:141501/70‑1 (MQ=255)
                                                     gAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAatca                                              >  1:3162746/1‑42 (MQ=255)
                                                       gTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATcgc                                          <  1:726336/44‑1 (MQ=255)
                                                       gTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa                                        >  1:696116/1‑46 (MQ=255)
                                                       gTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGc               <  1:812098/71‑1 (MQ=255)
                                                        tGCTTATTTTTGTATTTCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGc               >  1:4424675/1‑70 (MQ=255)
                                                        tGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGAt                                       <  1:1850571/46‑1 (MQ=255)
                                                        tGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGAt                                       <  1:3392378/46‑1 (MQ=255)
                                                        tGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGAt                                       <  1:2991637/46‑1 (MQ=255)
                                                        tGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGAt                                       <  1:3711333/46‑1 (MQ=255)
                                                        tGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt                     <  1:4820180/64‑1 (MQ=255)
                                                         gCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGa                                 <  1:2854505/51‑1 (MQ=255)
                                                          cTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCtt                         <  1:4868967/58‑1 (MQ=255)
                                                          cTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaa            <  1:502164/71‑1 (MQ=255)
                                                          cTGATCTTCGTCTCTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCa             >  1:5255712/1‑70 (MQ=255)
                                                           tGATCTTCGTCTTTCTGGTGATTTTTTATGGAatca                                              >  1:5554549/1‑36 (MQ=255)
                                                           tGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACa                               >  1:743848/1‑51 (MQ=255)
                                                           tGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGc               <  1:1624129/67‑1 (MQ=255)
                                                             aTCTTCGTCTTTCTGGTGATTTTTTATGGAatcatc                                            <  1:3542428/36‑1 (MQ=255)
                                                             aTCTTCGTCTTTCTGGTGATTTTTTATGGAatcat                                             >  1:892416/1‑35 (MQ=255)
                                                             aTCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaa           <  1:806317/69‑1 (MQ=255)
                                                              tCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg        >  1:4984488/1‑71 (MQ=255)
                                                               cTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGAt                  >  1:1338825/1‑60 (MQ=255)
                                                               cTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGAt                  >  1:865341/1‑60 (MQ=255)
                                                                 tCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa                                        >  1:3704124/1‑36 (MQ=255)
                                                                  cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa                                        >  1:2987201/1‑35 (MQ=255)
                                                                  cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaaa         <  1:5167353/66‑1 (MQ=255)
                                                                  cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgcgc     <  1:467712/70‑1 (MQ=255)
                                                                   gTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCa    <  1:5359199/70‑1 (MQ=255)
                                                                   gTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCa    <  1:789457/70‑1 (MQ=255)
                                                                    tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATccc                           <  1:4018103/46‑1 (MQ=255)
                                                                    tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgcgc     <  1:4690396/68‑1 (MQ=255)
                                                                    tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAc  >  1:782962/1‑71 (MQ=255)
                                                                    tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAc  <  1:851204/71‑1 (MQ=255)
                                                                     |                                                                     
CACTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAC  >  Exported/2569703‑2569841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: