Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,569,784 T→C 10.6% I18I (ATT→ATC yibI → predicted inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,569,7840TC10.6% 397.2 / 28.9 150I18I (ATT→ATCyibIpredicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (58/76);  new base C (11/5);  total (69/81)
Fisher's exact test for biased strand distribution p-value = 6.55e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01

Only 100 of 169 total aligned reads displayed.

AATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATG  >  Exported/2569717‑2569851
                                                                   |                                                                   
aaTCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAttttt                                                                  <  1:945975/71‑1 (MQ=255)
aaTCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAtttt                                                                   <  1:3528104/70‑1 (MQ=255)
 aTCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAtttttt                                                                 <  1:5032271/71‑1 (MQ=255)
 aTCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAtttttt                                                                 <  1:3006193/71‑1 (MQ=255)
 aTCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAttttt                                                                  >  1:687717/1‑70 (MQ=255)
    cGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATg                                                              >  1:313801/1‑71 (MQ=255)
    cGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATg                                                              >  1:2014749/1‑71 (MQ=255)
      aGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATgg                                                             >  1:3293467/1‑70 (MQ=255)
      aGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGa                                                            <  1:1007464/71‑1 (MQ=255)
       ggCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGaa                                                           <  1:4966574/71‑1 (MQ=255)
         cAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATgg                                                             <  1:2975868/67‑1 (MQ=255)
         cAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAatc                                                         <  1:4377304/71‑1 (MQ=255)
           gATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAatcat                                                       >  1:3468664/1‑71 (MQ=255)
           aatatGTTCCTGGACTATTTTGCACTGGGAGTGCTTATTTTTGTATTTCTGGTGATCTTCTATGGGatcat                                                       >  1:70260/2‑71 (MQ=255)
            atatGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAatcatc                                                      <  1:4388811/71‑1 (MQ=255)
             tatGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAttttt                                                                  <  1:5279947/58‑1 (MQ=255)
              atGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATcgc                                                    >  1:2824968/1‑71 (MQ=255)
               tGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTa                                                                >  1:417653/1‑58 (MQ=255)
               tGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATcgc                                                    <  1:4112691/70‑1 (MQ=255)
                 tttCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAtttttt                                                                 <  1:260697/55‑1 (MQ=255)
                   tCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATg                                                              <  1:5540174/56‑1 (MQ=255)
                   tCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATa                                                <  1:5518815/70‑1 (MQ=255)
                    cTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACa                                              >  1:5613240/1‑71 (MQ=255)
                       aaCTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTAt                                                               <  1:5147232/51‑1 (MQ=255)
                       aaCTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATg                                                              >  1:1265138/1‑52 (MQ=255)
                       aaCTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACAt                                             <  1:1428015/69‑1 (MQ=255)
                       aaCTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGa                                           <  1:5066146/71‑1 (MQ=255)
                          tATTTTGCACTGGGAGTGCTTATTTTTGTATTTCTGGTGAtcttc                                                                  <  1:811979/45‑2 (MQ=255)
                          tATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAtttt                                                                   <  1:4214106/44‑1 (MQ=255)
                          tATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATgg                                                             <  1:1138397/50‑1 (MQ=255)
                          tATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATgg                                                             <  1:4727819/50‑1 (MQ=255)
                            tttCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTa                                                                >  1:525027/1‑45 (MQ=255)
                              tCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCt                                    >  1:320574/1‑71 (MQ=255)
                               cgcgTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATgg                                                             <  1:1957759/45‑1 (MQ=255)
                               cgcgTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATgg                                                             <  1:4756952/45‑1 (MQ=255)
                               cgcgTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCt                                    >  1:2503064/1‑70 (MQ=255)
                                  gTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATc                                >  1:1804155/1‑71 (MQ=255)
                                   ttGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTAt                                                               <  1:5277875/39‑1 (MQ=255)
                                   ttGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTAt                                                               <  1:2308373/39‑1 (MQ=255)
                                   ttGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt                               <  1:2252363/71‑1 (MQ=255)
                                   ctGGGAGTGCTTATTTTTGTATTTCTGGTGATCTTCTATGGGatcat                                                       <  1:2136477/46‑1 (MQ=255)
                                    tGGGAGTGCTGATCTTCGTCTTTCTGGTGAttttt                                                                  <  1:3678832/35‑1 (MQ=255)
                                    tGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt                               >  1:644274/1‑70 (MQ=255)
                                     gggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTa                                                                >  1:1899228/1‑36 (MQ=255)
                                     gggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTa                                                                >  1:259420/1‑36 (MQ=255)
                                     gggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTg                              <  1:552288/70‑1 (MQ=255)
                                      ggAGTGCTTATTTTTGTATTTCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGAt                            >  1:4515618/1‑71 (MQ=255)
                                      ggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGa                             <  1:141501/70‑1 (MQ=255)
                                      ggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGa                             <  1:222583/70‑1 (MQ=255)
                                      ggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGa                             <  1:5462382/70‑1 (MQ=255)
                                         gTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATcgc                                                    <  1:726336/44‑1 (MQ=255)
                                          tGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGAt                                                 <  1:3711333/46‑1 (MQ=255)
                                          tGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGAt                                                 <  1:1103671/46‑1 (MQ=255)
                                          tGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGAt                                                 <  1:1850571/46‑1 (MQ=255)
                                          tGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGAt                                                 <  1:3392378/46‑1 (MQ=255)
                                          tGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGAt                                                 <  1:2991637/46‑1 (MQ=255)
                                           gCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGa                                           <  1:2854505/51‑1 (MQ=255)
                                           gCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt                               <  1:2916058/63‑1 (MQ=255)
                                            cTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCtt                                   <  1:4868967/58‑1 (MQ=255)
                                            cTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaa                      <  1:502164/71‑1 (MQ=255)
                                             tGATCTTCGTCTTTCTGGTGATTTTTTATGGAatca                                                        >  1:5554549/1‑36 (MQ=255)
                                             tGATCTTCGTCTTTCTGGTGATTTTTTATGGAatca                                                        >  1:2386579/1‑36 (MQ=255)
                                             tGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACa                                         >  1:815750/1‑51 (MQ=255)
                                             tGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGc                         <  1:777398/67‑1 (MQ=255)
                                             tGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGc                         <  1:1624129/67‑1 (MQ=255)
                                               aTCTTCGTCTTTCTGGTGATTTTTTATGGAatcatc                                                      <  1:791445/36‑1 (MQ=255)
                                               aTCTTCGTCTTTCTGGTGATTTTTTATGGAatcat                                                       >  1:892416/1‑35 (MQ=255)
                                                tCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                  >  1:3244098/1‑71 (MQ=255)
                                                 cTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGAt                            >  1:1338825/1‑60 (MQ=255)
                                                   tCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa                                                  >  1:3704124/1‑36 (MQ=255)
                                                    cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaaa                   <  1:5167353/66‑1 (MQ=255)
                                                    cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgcgc               >  1:3327599/1‑70 (MQ=255)
                                                     gtatttCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTaa             >  1:1521763/4‑71 (MQ=255)
                                                     gTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCa              <  1:5359199/70‑1 (MQ=255)
                                                     gTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCa              <  1:789457/70‑1 (MQ=255)
                                                      tatttCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTaa             <  1:27112/68‑1 (MQ=255)
                                                      tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt                               <  1:4594536/52‑1 (MQ=255)
                                                      tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgcgc               <  1:4991647/68‑1 (MQ=255)
                                                      tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgcgc               <  1:4690396/68‑1 (MQ=255)
                                                      tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgcgc               <  1:735993/68‑1 (MQ=255)
                                                      tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAc            <  1:851204/71‑1 (MQ=255)
                                                       cTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAc            >  1:1727243/1‑70 (MQ=255)
                                                       atttCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTaat            >  1:253195/2‑69 (MQ=255)
                                                        tttCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCa              <  1:4724949/67‑1 (MQ=255)
                                                          tCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAcc           <  1:3938435/68‑1 (MQ=255)
                                                          tCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCa          <  1:2648889/69‑1 (MQ=255)
                                                          tCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATc        >  1:585691/1‑71 (MQ=255)
                                                          tCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCaaaaaa                  >  1:1709133/1‑60 (MQ=255)
                                                          tCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTaa             >  1:1493973/1‑66 (MQ=255)
                                                          tCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTAatcatc        >  1:2103782/1‑71 (MQ=255)
                                                          tCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTAatcatc        >  1:4578061/1‑71 (MQ=255)
                                                              gTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcccc     <  1:2434928/70‑1 (MQ=255)
                                                              gTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATccc      <  1:2765947/69‑1 (MQ=255)
                                                              gTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTAATCATCCTCa    <  1:1255475/71‑1 (MQ=255)
                                                              gTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTAATCATCCTCa    <  1:3876165/71‑1 (MQ=255)
                                                               tGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCAt   >  1:4860180/1‑71 (MQ=255)
                                                                 aTTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaa                    >  1:4173963/1‑52 (MQ=255)
                                                                 aTTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATg  <  1:4031945/70‑1 (MQ=255)
                                                                   tttttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaa                    <  1:1553245/50‑1 (MQ=255)
                                                                   tttttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaa                    <  1:2109396/50‑1 (MQ=255)
                                                                   |                                                                   
AATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATG  >  Exported/2569717‑2569851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: