Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,569,787 T→C 10.6% F19F (TTT→TTC yibI → predicted inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,569,7870TC10.6% 399.6 / 29.5 150F19F (TTT→TTCyibIpredicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (58/76);  new base C (11/5);  total (69/81)
Fisher's exact test for biased strand distribution p-value = 6.55e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 180 total aligned reads displayed.

AATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACG  >  Exported/2569717‑2569857
                                                                      |                                                                      
aaTCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAttttt                                                                        <  1:5611426/71‑1 (MQ=255)
aaTCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAttttt                                                                        >  1:3293845/1‑71 (MQ=255)
aaTCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAttttt                                                                        <  1:945975/71‑1 (MQ=255)
 aTCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAtttttt                                                                       <  1:5032271/71‑1 (MQ=255)
 aTCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAtttttt                                                                       <  1:3006193/71‑1 (MQ=255)
  tCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTa                                                                      >  1:3368057/1‑71 (MQ=255)
      aGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATgg                                                                   >  1:3293467/1‑70 (MQ=255)
      aGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGa                                                                  <  1:1007464/71‑1 (MQ=255)
       ggCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGaa                                                                 <  1:5426406/71‑1 (MQ=255)
         cAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATgg                                                                   <  1:2975868/67‑1 (MQ=255)
         cAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAatc                                                               <  1:4377304/71‑1 (MQ=255)
           gATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAatcat                                                             >  1:3468664/1‑71 (MQ=255)
            atatGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAatcatc                                                            <  1:4388811/71‑1 (MQ=255)
               tGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATcgc                                                          <  1:4112691/70‑1 (MQ=255)
                  ttCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTa                                                                      >  1:1518927/1‑55 (MQ=255)
                  ttCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa                                                        >  1:724577/1‑69 (MQ=255)
                  ttCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa                                                        >  1:1112452/1‑69 (MQ=255)
                   tCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATg                                                                    <  1:5540174/56‑1 (MQ=255)
                   tCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATa                                                      <  1:5518815/70‑1 (MQ=255)
                    cTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACa                                                    >  1:5613240/1‑71 (MQ=255)
                     tAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACa                                                    >  1:1577962/1‑70 (MQ=255)
                     tAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACAt                                                   >  1:1824320/1‑71 (MQ=255)
                       aaCTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTAt                                                                     <  1:5147232/51‑1 (MQ=255)
                       aaCTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATg                                                                    >  1:1265138/1‑52 (MQ=255)
                       aaCTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACAt                                                   <  1:1428015/69‑1 (MQ=255)
                       aaCTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACAt                                                   <  1:3771123/69‑1 (MQ=255)
                          tATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATgg                                                                   <  1:1138397/50‑1 (MQ=255)
                          tATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATgg                                                                   <  1:1665762/50‑1 (MQ=255)
                            tttCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTa                                                                      >  1:4201287/1‑45 (MQ=255)
                            tttCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTa                                                                      >  1:525027/1‑45 (MQ=255)
                            tttCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATg                                                                    <  1:2016093/47‑1 (MQ=255)
                             ttCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAatc                                                               >  1:217316/1‑51 (MQ=255)
                               cgcgTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATgg                                                                   <  1:2830359/45‑1 (MQ=255)
                               cgcgTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCt                                          >  1:1312826/1‑70 (MQ=255)
                                gcgTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCtt                                         <  1:1162472/70‑1 (MQ=255)
                                  gTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATc                                      >  1:1804155/1‑71 (MQ=255)
                                   ttGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTAt                                                                     <  1:2308373/39‑1 (MQ=255)
                                   ttGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTAt                                                                     <  1:5277875/39‑1 (MQ=255)
                                   ctGGGAGTGCTTATTTTTGTATTTCTGGTGATCTTCTATGGGatcat                                                             <  1:3523727/46‑1 (MQ=255)
                                    tGGGAGTGCTGATCTTCGTCTTTCTGGTGAttttt                                                                        <  1:3678832/35‑1 (MQ=255)
                                    tGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATAc                                                     >  1:4174044/1‑54 (MQ=255)
                                    tGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt                                     >  1:644274/1‑70 (MQ=255)
                                     gggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTa                                                                      >  1:259420/1‑36 (MQ=255)
                                     gggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGa                                                                  <  1:5356389/40‑1 (MQ=255)
                                     gggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTg                                    <  1:552288/70‑1 (MQ=255)
                                      ggAGTGCTTATTTTTGTATTTCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGAt                                  >  1:4515618/1‑71 (MQ=255)
                                       gAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAatca                                                              >  1:3162746/1‑42 (MQ=255)
                                         gTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATcgc                                                          <  1:726336/44‑1 (MQ=255)
                                         gTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa                                                        >  1:2265264/1‑46 (MQ=255)
                                         gTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa                                                        >  1:696116/1‑46 (MQ=255)
                                         gTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGc                               <  1:812098/71‑1 (MQ=255)
                                          tGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGAt                                                       <  1:2991637/46‑1 (MQ=255)
                                          tGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt                                     <  1:4820180/64‑1 (MQ=255)
                                           gCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt                                     <  1:1895950/63‑1 (MQ=255)
                                           gCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt                                     <  1:2916058/63‑1 (MQ=255)
                                            cTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCtt                                         <  1:4868967/58‑1 (MQ=255)
                                            cTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaa                            <  1:502164/71‑1 (MQ=255)
                                             tGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACa                                               >  1:743848/1‑51 (MQ=255)
                                               aTCTTCGTCTTTCTGGTGATTTTTTATGGAatcatc                                                            <  1:791445/36‑1 (MQ=255)
                                               aTCTTCGTCTTTCTGGTGATTTTTTATGGAatcat                                                             >  1:892416/1‑35 (MQ=255)
                                               aTCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaa                           <  1:806317/69‑1 (MQ=255)
                                                tCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                        >  1:3244098/1‑71 (MQ=255)
                                                tCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                        >  1:4984488/1‑71 (MQ=255)
                                                   tCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa                                                        >  1:3710906/1‑36 (MQ=255)
                                                    cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaaa                         <  1:5167353/66‑1 (MQ=255)
                                                    cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaaa                         <  1:1358557/66‑1 (MQ=255)
                                                    cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaaa                         <  1:2519598/66‑1 (MQ=255)
                                                    cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgcgc                     <  1:467712/70‑1 (MQ=255)
                                                     gtatttCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTaa                   >  1:1521763/4‑71 (MQ=255)
                                                      tatttCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTaa                   <  1:27112/68‑1 (MQ=255)
                                                      tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATccc                                           <  1:4018103/46‑1 (MQ=255)
                                                      tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt                                     <  1:1982204/52‑1 (MQ=255)
                                                      tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAc                  <  1:851204/71‑1 (MQ=255)
                                                      tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAc                  >  1:2122634/1‑71 (MQ=255)
                                                      tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAc                  >  1:782962/1‑71 (MQ=255)
                                                       cTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAc                  >  1:1727243/1‑70 (MQ=255)
                                                       atttCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTaat                  >  1:253195/2‑69 (MQ=255)
                                                        tttCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCa                    <  1:4724949/67‑1 (MQ=255)
                                                        tttCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCa                >  1:2794752/1‑71 (MQ=255)
                                                        tttCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCa                >  1:2630246/1‑71 (MQ=255)
                                                        tttCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTAATCa                >  1:4881032/1‑71 (MQ=255)
                                                          tCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAcc                 <  1:3938435/68‑1 (MQ=255)
                                                          tCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAcc                 <  1:4721837/68‑1 (MQ=255)
                                                          tCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATc              >  1:585691/1‑71 (MQ=255)
                                                          tCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTAatcatc              >  1:2103782/1‑71 (MQ=255)
                                                          tCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTAatcatc              >  1:4578061/1‑71 (MQ=255)
                                                              gTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcccc           <  1:2434928/70‑1 (MQ=255)
                                                              gTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcccc           <  1:4729617/70‑1 (MQ=255)
                                                               tGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCAt         >  1:2705846/1‑71 (MQ=255)
                                                                 aTTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATg        <  1:4031945/70‑1 (MQ=255)
                                                                    ttttATGGAATCATCGCGATACATGACATCCCTTATCTGa                                   >  1:1020948/1‑40 (MQ=255)
                                                                    ttttATGGAATCATCGCGATACATGACATCCCTTATCTGa                                   >  1:2328510/1‑40 (MQ=255)
                                                                    ttttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                        >  1:190146/1‑51 (MQ=255)
                                                                     tttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                        >  1:224250/1‑50 (MQ=255)
                                                                     tttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcc             >  1:5449866/1‑61 (MQ=255)
                                                                     tttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcc             >  1:3092708/1‑61 (MQ=255)
                                                                      ttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                        >  1:3549900/1‑49 (MQ=255)
                                                                      ttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                        >  1:2437681/1‑49 (MQ=255)
                                                                      ttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGAc   >  1:5043405/1‑70 (MQ=255)
                                                                      ttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACg  >  1:1469594/1‑71 (MQ=255)
                                                                      |                                                                      
AATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACG  >  Exported/2569717‑2569857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: