Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,569,793 A→G 11.2% G21G (GGA→GGG yibI → predicted inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,569,7930AG11.2% 372.6 / 29.6 143G21G (GGA→GGGyibIpredicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (65/62);  new base G (11/5);  total (76/67)
Fisher's exact test for biased strand distribution p-value = 2.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

Only 100 of 150 total aligned reads displayed.

AGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATT  >  Exported/2569723‑2569862
                                                                      |                                                                     
aGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGa                                                                       <  1:1007464/71‑1 (MQ=255)
 ggCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGaa                                                                      <  1:4966574/71‑1 (MQ=255)
   cAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAatc                                                                    <  1:4377304/71‑1 (MQ=255)
     gATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAatcat                                                                  >  1:3468664/1‑71 (MQ=255)
     aatatGTTCCTGGACTATTTTGCACTGGGAGTGCTTATTTTTGTATTTCTGGTGATCTTCTATGGGatcat                                                                  >  1:70260/2‑71 (MQ=255)
      atatGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAatcatc                                                                 <  1:4388811/71‑1 (MQ=255)
        atGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATcgc                                                               >  1:2824968/1‑71 (MQ=255)
         tGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATcgc                                                               <  1:4112691/70‑1 (MQ=255)
            ttCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa                                                             >  1:1112452/1‑69 (MQ=255)
             tCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATa                                                           <  1:5518815/70‑1 (MQ=255)
              cTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACa                                                         >  1:5613240/1‑71 (MQ=255)
               tAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACa                                                         >  1:1577962/1‑70 (MQ=255)
               tAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACAt                                                        >  1:1824320/1‑71 (MQ=255)
                 aaCTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACAt                                                        <  1:1428015/69‑1 (MQ=255)
                       ttCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAatc                                                                    >  1:217316/1‑51 (MQ=255)
                          gcgTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCtt                                              <  1:1162472/70‑1 (MQ=255)
                            gTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATc                                           >  1:1804155/1‑71 (MQ=255)
                             ttGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt                                          <  1:2252363/71‑1 (MQ=255)
                             ctGGGAGTGCTTATTTTTGTATTTCTGGTGATCTTCTATGGGatcat                                                                  <  1:2136477/46‑1 (MQ=255)
                              tGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGa                                                      >  1:58755/1‑58 (MQ=255)
                               gggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGa                                                                       <  1:5356389/40‑1 (MQ=255)
                               gggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTg                                         <  1:552288/70‑1 (MQ=255)
                                ggAGTGCTTATTTTTGTATTTCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGAt                                       >  1:4515618/1‑71 (MQ=255)
                                ggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGa                                        <  1:5462382/70‑1 (MQ=255)
                                 gAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAatca                                                                   >  1:3162746/1‑42 (MQ=255)
                                   gTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATcgc                                                               <  1:726336/44‑1 (MQ=255)
                                   gTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa                                                             >  1:696116/1‑46 (MQ=255)
                                   gTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGc                                    <  1:812098/71‑1 (MQ=255)
                                    tGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGAt                                                            <  1:3711333/46‑1 (MQ=255)
                                    tGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGAt                                                            <  1:2991637/46‑1 (MQ=255)
                                    tGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt                                          <  1:4820180/64‑1 (MQ=255)
                                     gCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGa                                                      <  1:2854505/51‑1 (MQ=255)
                                     gCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt                                          <  1:1895950/63‑1 (MQ=255)
                                      cTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaa                                 <  1:502164/71‑1 (MQ=255)
                                       tGATCTTCGTCTTTCTGGTGATTTTTTATGGAatca                                                                   >  1:5554549/1‑36 (MQ=255)
                                       tGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACa                                                    >  1:815750/1‑51 (MQ=255)
                                       tGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGc                                    <  1:777398/67‑1 (MQ=255)
                                         aTCTTCGTCTTTCTGGTGATTTTTTATGGAatcatc                                                                 <  1:3542428/36‑1 (MQ=255)
                                         aTCTTCGTCTTTCTGGTGATTTTTTATGGAatcatc                                                                 <  1:791445/36‑1 (MQ=255)
                                         aTCTTCGTCTTTCTGGTGATTTTTTATGGAatcat                                                                  >  1:892416/1‑35 (MQ=255)
                                         aTCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTAGCTGATTGCCaaa                                >  1:5178328/1‑69 (MQ=255)
                                          tCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                             >  1:3244098/1‑71 (MQ=255)
                                           cTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGAt                                       >  1:1338825/1‑60 (MQ=255)
                                           cTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGAt                                       >  1:865341/1‑60 (MQ=255)
                                             tCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa                                                             >  1:3710906/1‑36 (MQ=255)
                                              cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa                                                             >  1:2987201/1‑35 (MQ=255)
                                              cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaaa                              <  1:5167353/66‑1 (MQ=255)
                                              cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaaa                              <  1:2519598/66‑1 (MQ=255)
                                              cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaaa                              <  1:1358557/66‑1 (MQ=255)
                                              cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgcgc                          >  1:3327599/1‑70 (MQ=255)
                                              cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgcgc                          <  1:467712/70‑1 (MQ=255)
                                               gtatttCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTaa                        >  1:1521763/4‑71 (MQ=255)
                                               gTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCa                         <  1:5359199/70‑1 (MQ=255)
                                                tatttCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTaa                        <  1:27112/68‑1 (MQ=255)
                                                tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATccc                                                <  1:4018103/46‑1 (MQ=255)
                                                tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt                                          <  1:4594536/52‑1 (MQ=255)
                                                tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt                                          <  1:1449400/52‑1 (MQ=255)
                                                tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgcgc                          <  1:735993/68‑1 (MQ=255)
                                                tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgcgc                          <  1:4991647/68‑1 (MQ=255)
                                                tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAc                       >  1:782962/1‑71 (MQ=255)
                                                 cTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAc                       >  1:1727243/1‑70 (MQ=255)
                                                 atttCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTaat                       >  1:253195/2‑69 (MQ=255)
                                                  tttCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCa                         <  1:4724949/67‑1 (MQ=255)
                                                  tttCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTAATCa                     >  1:4881032/1‑71 (MQ=255)
                                                    tCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAcc                      <  1:3938435/68‑1 (MQ=255)
                                                    tCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCa                     <  1:2648889/69‑1 (MQ=255)
                                                    tCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATc                   >  1:585691/1‑71 (MQ=255)
                                                    tCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCaaaaaa                             >  1:1709133/1‑60 (MQ=255)
                                                    tCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTaa                        >  1:1493973/1‑66 (MQ=255)
                                                    tCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTAatcatc                   >  1:2103782/1‑71 (MQ=255)
                                                        gTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcccc                <  1:4729617/70‑1 (MQ=255)
                                                        gTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcccc                <  1:2434928/70‑1 (MQ=255)
                                                        gTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATccc                 <  1:2765947/69‑1 (MQ=255)
                                                        gTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTAATCATCCTCa               <  1:3876165/71‑1 (MQ=255)
                                                        gTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTAATCATCCTCa               <  1:1255475/71‑1 (MQ=255)
                                                         tGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCAt              >  1:5042565/1‑71 (MQ=255)
                                                         tGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCAt              >  1:4860180/1‑71 (MQ=255)
                                                           aTTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaa                               >  1:4173963/1‑52 (MQ=255)
                                                           aTTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATg             <  1:4031945/70‑1 (MQ=255)
                                                             tttttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaa                               <  1:2319478/50‑1 (MQ=255)
                                                             tttttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaa                               <  1:2109396/50‑1 (MQ=255)
                                                              ttttATGGAATCATCGCGATACATGACATCCCTTATCTGa                                        >  1:1020948/1‑40 (MQ=255)
                                                              ttttATGGAATCATCGCGATACATGACATCCCTTATCTGa                                        >  1:1130441/1‑40 (MQ=255)
                                                              ttttATGGAATCATCGCGATACATGACATCCCTTATCTGa                                        >  1:2328510/1‑40 (MQ=255)
                                                              ttttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                             >  1:190146/1‑51 (MQ=255)
                                                               tttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                             >  1:224250/1‑50 (MQ=255)
                                                               tttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcc                  >  1:5449866/1‑61 (MQ=255)
                                                               tttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcc                  >  1:3092708/1‑61 (MQ=255)
                                                               tttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcc                  >  1:3497020/1‑61 (MQ=255)
                                                                ttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                             >  1:3549900/1‑49 (MQ=255)
                                                                ttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                             >  1:2437681/1‑49 (MQ=255)
                                                                ttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGAc        >  1:5043405/1‑70 (MQ=255)
                                                                ttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACg       >  1:797799/1‑71 (MQ=255)
                                                                   tGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa    <  1:2605352/71‑1 (MQ=255)
                                                                     gAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                             >  1:4703683/1‑44 (MQ=255)
                                                                     gAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                             >  1:2644590/1‑44 (MQ=255)
                                                                     gAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAtt  >  1:4691944/1‑71 (MQ=255)
                                                                     gAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAtt  >  1:2599164/1‑71 (MQ=255)
                                                                      aaTCATCGCGATACATGACATCCCTTATCTGATTGCCaaaaa                              >  1:4549641/1‑42 (MQ=255)
                                                                      aaTCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGcc           <  1:1603628/61‑1 (MQ=255)
                                                                      |                                                                     
AGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATT  >  Exported/2569723‑2569862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: