Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 311,784 G→T 10.6% R363R (CGC→CGA acrB ← multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported311,7840GT10.6% 357.7 / 14.9 120R363R (CGC→CGAacrBmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base G (45/61);  new base T (7/6);  total (52/68)
Fisher's exact test for biased strand distribution p-value = 5.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 125 total aligned reads displayed.

CAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAAC  >  Exported/311714‑311852
                                                                      |                                                                    
   aGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTcgcgcg                                                                    >  1:4846186/1‑70 (MQ=255)
   aGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCgg                                                                   <  1:3663923/71‑1 (MQ=255)
    ggcggcAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGa                                                                  <  1:2655864/71‑1 (MQ=255)
     gcggcAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCACCGTCGCGCgg                                                                   >  1:3497152/1‑69 (MQ=255)
          aaGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCt                                                            <  1:1388741/71‑1 (MQ=255)
           aGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTg                                                           <  1:1954891/71‑1 (MQ=255)
           aGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTg                                                           >  1:2082518/1‑71 (MQ=255)
           aGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTg                                                           >  1:2930044/1‑71 (MQ=255)
           aGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGGCGGAATCAACGTCGCGCGGAAGTTCTg                                                           <  1:1104062/71‑1 (MQ=255)
           aGGACGGCAAAGGTCCCGAGAAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTg                                                           >  1:3748501/1‑71 (MQ=255)
                ggCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGa                                                      <  1:3631523/71‑1 (MQ=255)
                  cAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAAc                                                    <  1:5176462/71‑1 (MQ=255)
                    aaGGTCCCCAGCAGCACCACCGGTACGGCGATGGTCGGGATCAGCGTGGCGCGGAAGTTCTGCAGGAACAg                                                  >  1:5237249/1‑71 (MQ=11)
                    aaGGTCCCCAGCAGCACCACCGGTACGGCGATGGTCGGGATCAGCGTGGCGCGGAAGTTCTGCAGGAACAg                                                  >  1:2637157/1‑71 (MQ=11)
                     aGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAg                                                                <  1:4473877/56‑1 (MQ=255)
                     aGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAg                                                                <  1:2496179/56‑1 (MQ=255)
                     aGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAg                                                                <  1:4773430/56‑1 (MQ=255)
                     aGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAg                                                                <  1:2961535/56‑1 (MQ=255)
                     aGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAg                                                                <  1:2566969/56‑1 (MQ=255)
                      ggTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGa                                                 >  1:1236659/1‑70 (MQ=255)
                          ccGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGaa                                                                 >  1:4441074/1‑50 (MQ=255)
                          ccGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACa                                                   <  1:122985/64‑1 (MQ=255)
                           cGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAcatacata                                           <  1:128063/71‑1 (MQ=255)
                           cGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATa                                           <  1:2566942/71‑1 (MQ=255)
                             aacAACACCACGGGTACCGCAATGGTGGGGATCAGAGTTGCTCGCATATTCTGCAAGAACAGATACATCAc                                         >  1:5222363/3‑71 (MQ=2)
                             aGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGaa                                                     >  1:2780819/1‑59 (MQ=255)
                             aGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAAc                                         <  1:2970212/71‑1 (MQ=255)
                             aGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAAc                                         <  1:966593/71‑1 (MQ=255)
                              gCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAAcc                                        <  1:1008852/71‑1 (MQ=255)
                              acAACACCACGGGTACCGCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCAc                                         >  1:1975921/2‑70 (MQ=2)
                              acAACACCACGGGTACCGCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCAc                                         >  1:1661494/2‑70 (MQ=2)
                                aacaccacgGGTACCGCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCACCAg                                      >  1:4596896/4‑71 (MQ=2)
                                aaTACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACAt                                            <  1:511256/65‑1 (MQ=255)
                                 acaccacgGGTACCGCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCACCAgg                                     <  1:4670260/69‑1 (MQ=9)
                                   accacgGGTACCGCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCACCAgg                                     <  1:1106224/69‑1 (MQ=9)
                                   accaccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGaa                                                                 >  1:2786853/1‑41 (MQ=255)
                                   accaccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGaa                                                                 >  1:822193/1‑41 (MQ=255)
                                    ccaccGGTACGGCAATGGTCGGATTCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGaa                                   >  1:4568543/1‑70 (MQ=255)
                                    ccaccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGaa                                                                 >  1:1165736/1‑40 (MQ=255)
                                    ccaccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGaa                                                                 >  1:1083101/1‑40 (MQ=255)
                                    ccaccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACAt                                            >  1:4393492/1‑61 (MQ=255)
                                    ccaccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAac                                  <  1:403968/71‑1 (MQ=255)
                                    ccaccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGAAGGAACAg                                                  >  1:2907685/1‑55 (MQ=255)
                                     caccGGTACGGCAATGGTCGGAATCAACGTCGCGCgg                                                                   >  1:5433506/1‑37 (MQ=255)
                                     caccGGTACGGCAATGGTCGGAATCAACGTCGCGCgg                                                                   >  1:4014668/1‑37 (MQ=255)
                                     caccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAAc                                                    <  1:3971975/52‑1 (MQ=255)
                                      acgggTACCGCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCACCAGGAacac                                <  1:5493422/68‑1 (MQ=9)
                                      accGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAgg                                     <  1:4882378/66‑1 (MQ=255)
                                      accGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAacac                                <  1:3584368/71‑1 (MQ=255)
                                      accGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAacac                                <  1:297754/71‑1 (MQ=255)
                                       ccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAg                                                  >  1:266931/1‑52 (MQ=255)
                                       ccGGTACGGCAATGGTAGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACa                                                   >  1:4796944/1‑51 (MQ=255)
                                            aCGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGgat                          >  1:4248622/1‑71 (MQ=255)
                                             ccgCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCACCAGGAACACCAGcat                          <  1:3775086/68‑1 (MQ=9)
                                             ccgCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCACCAGGAACACCAGcat                          <  1:786931/68‑1 (MQ=9)
                                             cGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGgatg                         <  1:4147432/71‑1 (MQ=255)
                                               gCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAAc                                                    >  1:2497879/1‑42 (MQ=255)
                                               gCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGgatgat                       <  1:1848565/71‑1 (MQ=255)
                                               gCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGgatgat                       <  1:5089969/71‑1 (MQ=255)
                                                cAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCAcc                                        <  1:1768456/53‑1 (MQ=2)
                                                 aaTGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCACCAGGACCACCAGcataatg                      >  1:5560352/1‑69 (MQ=2)
                                                 aaTGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATaa                                          >  1:2928644/1‑50 (MQ=255)
                                                  aTGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGa                                                 >  1:1807280/1‑42 (MQ=255)
                                                  aTGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACAt                                            <  1:1821335/47‑1 (MQ=255)
                                                  aTGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACAt                                            <  1:1387417/47‑1 (MQ=255)
                                                  aTGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATa                                           <  1:2646836/48‑1 (MQ=255)
                                                  aTGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACg                               <  1:2180989/60‑1 (MQ=255)
                                                   tGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGaa                                                     >  1:1701577/1‑37 (MQ=255)
                                                   tGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAgg                                     <  1:1423131/53‑1 (MQ=255)
                                                   tGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAg                             <  1:1524122/61‑1 (MQ=255)
                                                   tGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAg                             <  1:3283076/61‑1 (MQ=255)
                                                   tGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAg                             <  1:3154050/61‑1 (MQ=255)
                                                   tGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAg                             <  1:2015780/61‑1 (MQ=255)
                                                   tGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAg                             <  1:5267957/61‑1 (MQ=255)
                                                    ggTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAg                             >  1:4168476/1‑60 (MQ=255)
                                                    ggTCGGAATCAACGTCGCGAGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCtt                  >  1:5645169/1‑71 (MQ=255)
                                                     gTCGGATTCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAg                             <  1:4376172/59‑1 (MQ=255)
                                                       cGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGa               >  1:1014996/1‑71 (MQ=255)
                                                          aaTCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCa            <  1:5031656/71‑1 (MQ=255)
                                                           aTCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAg           >  1:1071591/1‑71 (MQ=255)
                                                            tCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGgatg                         <  1:2094437/56‑1 (MQ=255)
                                                              aaCGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCg         <  1:3908644/70‑1 (MQ=255)
                                                                cGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAgg                                     <  1:4869205/40‑1 (MQ=255)
                                                                cGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGttt      >  1:5371946/1‑71 (MQ=255)
                                                                cGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGttt      >  1:2748303/1‑71 (MQ=255)
                                                                cGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGttt      >  1:2754883/1‑71 (MQ=255)
                                                                cGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGttt      >  1:1417996/1‑71 (MQ=255)
                                                                 gTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGtttt     <  1:950230/71‑1 (MQ=255)
                                                                 gTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGtttt     <  1:3121986/71‑1 (MQ=255)
                                                                 gTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGtttt     <  1:1484380/71‑1 (MQ=255)
                                                                 gTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGtttt     <  1:4426564/71‑1 (MQ=255)
                                                                 gTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGtttt     <  1:4208913/71‑1 (MQ=255)
                                                                 gTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGtttt     <  1:3234591/71‑1 (MQ=255)
                                                                  tCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCg         <  1:1937636/66‑1 (MQ=255)
                                                                  tCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTa    <  1:1121907/71‑1 (MQ=255)
                                                                  tCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTa    <  1:3918965/71‑1 (MQ=255)
                                                                  tCGCGCGGAAGTTCTGAAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCg         <  1:2248828/66‑1 (MQ=255)
                                                                    gcgcgGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTaa   >  1:1197818/1‑70 (MQ=255)
                                                                    gcgcgGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTaa   >  1:3370189/1‑70 (MQ=255)
                                                                    gcgcgGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAAc  <  1:2932654/71‑1 (MQ=255)
                                                                      |                                                                    
CAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAAC  >  Exported/311714‑311852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: