Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,569,801 C→T 8.5% A24V (GCG→GTG) ‡ yibI → predicted inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,569,8010CT 8.5% 400.3 / 18.3 140A24V (GCG→GTG) ‡yibIpredicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (65/63);  new base T (9/3);  total (74/66)
Fisher's exact test for biased strand distribution p-value = 1.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.97e-01

Only 100 of 154 total aligned reads displayed.

ATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGC  >  Exported/2569731‑2569870
                                                                      |                                                                     
atGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATcgc                                                                       >  1:2824968/1‑71 (MQ=255)
 tGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATcgc                                                                       <  1:571171/70‑1 (MQ=255)
 tGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATcgc                                                                       <  1:4112691/70‑1 (MQ=255)
    ttCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa                                                                     >  1:1112452/1‑69 (MQ=255)
    ttCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa                                                                     >  1:724577/1‑69 (MQ=255)
     tCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATa                                                                   <  1:5518815/70‑1 (MQ=255)
      cTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACa                                                                 >  1:5613240/1‑71 (MQ=255)
       tAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACa                                                                 >  1:1577962/1‑70 (MQ=255)
         aaCTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGTTGATTTTTTATGGAATCATCGCGATACATGa                                                              <  1:4099609/71‑1 (MQ=255)
         aaCTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACAt                                                                <  1:1428015/69‑1 (MQ=255)
                tCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCt                                                       >  1:320574/1‑71 (MQ=255)
                 cgcgTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCt                                                       >  1:2503064/1‑70 (MQ=255)
                 cgcgTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCt                                                       >  1:1312826/1‑70 (MQ=255)
                  gcgTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCtt                                                      <  1:1162472/70‑1 (MQ=255)
                    gTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATc                                                   >  1:1804155/1‑71 (MQ=255)
                     ttGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt                                                  <  1:2252363/71‑1 (MQ=255)
                      tGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATAc                                                                  >  1:4174044/1‑54 (MQ=255)
                      tGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGa                                                              >  1:58755/1‑58 (MQ=255)
                       gggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTg                                                 <  1:552288/70‑1 (MQ=255)
                        ggAGTGCTTATTTTTGTATTTCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGAt                                               >  1:4515618/1‑71 (MQ=255)
                        ggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGa                                                <  1:141501/70‑1 (MQ=255)
                        ggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGa                                                <  1:222583/70‑1 (MQ=255)
                           gTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa                                                                     >  1:696116/1‑46 (MQ=255)
                           gTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa                                                                     >  1:2265264/1‑46 (MQ=255)
                           gTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGc                                            <  1:812098/71‑1 (MQ=255)
                            tGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGAt                                                                    <  1:3711333/46‑1 (MQ=255)
                            tGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGAt                                                                    <  1:1850571/46‑1 (MQ=255)
                            tGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt                                                  <  1:4820180/64‑1 (MQ=255)
                             gCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt                                                  <  1:1895950/63‑1 (MQ=255)
                             gCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt                                                  <  1:2916058/63‑1 (MQ=255)
                              cTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaa                                         <  1:502164/71‑1 (MQ=255)
                               tGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACa                                                            >  1:743848/1‑51 (MQ=255)
                                 aTCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaa                                        <  1:806317/69‑1 (MQ=255)
                                  tCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                                     >  1:4984488/1‑71 (MQ=255)
                                   cTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGAt                                               >  1:1338825/1‑60 (MQ=255)
                                     tCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa                                                                     >  1:3704124/1‑36 (MQ=255)
                                      cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa                                                                     >  1:2987201/1‑35 (MQ=255)
                                      cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaaa                                      <  1:5167353/66‑1 (MQ=255)
                                      cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgcgc                                  <  1:467712/70‑1 (MQ=255)
                                      cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgcgc                                  >  1:3327599/1‑70 (MQ=255)
                                       gtatttCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTaa                                >  1:1521763/4‑71 (MQ=255)
                                       gTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCa                                 <  1:789457/70‑1 (MQ=255)
                                       gTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCa                                 <  1:5359199/70‑1 (MQ=255)
                                        tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATccc                                                        <  1:4018103/46‑1 (MQ=255)
                                        tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt                                                  <  1:1982204/52‑1 (MQ=255)
                                        tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt                                                  <  1:4594536/52‑1 (MQ=255)
                                        tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgcgc                                  <  1:735993/68‑1 (MQ=255)
                                        tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAc                               <  1:851204/71‑1 (MQ=255)
                                        tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAc                               >  1:2122634/1‑71 (MQ=255)
                                        tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAc                               >  1:782962/1‑71 (MQ=255)
                                         cTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAc                               >  1:1727243/1‑70 (MQ=255)
                                         atttCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTaat                               >  1:253195/2‑69 (MQ=255)
                                          tttCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCa                                 <  1:4724949/67‑1 (MQ=255)
                                          tttCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCa                             >  1:2794752/1‑71 (MQ=255)
                                          tttCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCa                             >  1:2630246/1‑71 (MQ=255)
                                          tttCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTAATCa                             >  1:4881032/1‑71 (MQ=255)
                                            tCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAcc                              <  1:3938435/68‑1 (MQ=255)
                                            tCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCaaaaaa                                     >  1:1709133/1‑60 (MQ=255)
                                            tCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTaa                                >  1:1493973/1‑66 (MQ=255)
                                                gTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcccc                        <  1:4729617/70‑1 (MQ=255)
                                                gTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATccc                         <  1:2765947/69‑1 (MQ=255)
                                                gTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTAATCATCCTCa                       <  1:3876165/71‑1 (MQ=255)
                                                gTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTAATCATCCTCa                       <  1:1255475/71‑1 (MQ=255)
                                                 tGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCAt                      >  1:2705846/1‑71 (MQ=255)
                                                 tGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCAt                      >  1:4860180/1‑71 (MQ=255)
                                                 tGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCAt                      >  1:5042565/1‑71 (MQ=255)
                                                     tttttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaa                                       <  1:2109396/50‑1 (MQ=255)
                                                      ttttATGGAATCATCGCGATACATGACATCCCTTATCTGa                                                >  1:2328510/1‑40 (MQ=255)
                                                      ttttATGGAATCATCGCGATACATGACATCCCTTATCTGa                                                >  1:1020948/1‑40 (MQ=255)
                                                      ttttATGGAATCATCGCGATACATGACATCCCTTATCTGa                                                >  1:1130441/1‑40 (MQ=255)
                                                       tttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcc                          >  1:3092708/1‑61 (MQ=255)
                                                       tttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcc                          >  1:5449866/1‑61 (MQ=255)
                                                       tttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcc                          >  1:3497020/1‑61 (MQ=255)
                                                        ttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                                     >  1:2437681/1‑49 (MQ=255)
                                                        ttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                                     >  1:3549900/1‑49 (MQ=255)
                                                        ttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGAc                >  1:5043405/1‑70 (MQ=255)
                                                        ttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACg               >  1:1469594/1‑71 (MQ=255)
                                                          aTGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGc              >  1:3002362/1‑70 (MQ=255)
                                                             gAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                                     >  1:4703683/1‑44 (MQ=255)
                                                             gAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                                     >  1:2644590/1‑44 (MQ=255)
                                                             gAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAtt          >  1:4691944/1‑71 (MQ=255)
                                                             gAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAtt          >  1:2599164/1‑71 (MQ=255)
                                                              aaTCATCGCGATACATGACATCCCTTATCTGATTGCCaaaaa                                      >  1:4549641/1‑42 (MQ=255)
                                                              aaTCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGcc                   <  1:1603628/61‑1 (MQ=255)
                                                                tcatcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAt           <  1:906763/67‑1 (MQ=255)
                                                                tcatcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAt           <  1:3408617/67‑1 (MQ=255)
                                                                tcatcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCa        >  1:3992792/1‑70 (MQ=255)
                                                                tcatcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCAt       <  1:1797360/71‑1 (MQ=255)
                                                                  atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                                     >  1:5237444/1‑39 (MQ=255)
                                                                  atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATa      >  1:852705/1‑70 (MQ=255)
                                                                   tcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCaa                                <  1:5087400/43‑1 (MQ=255)
                                                                   tcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACg               >  1:1004806/1‑60 (MQ=255)
                                                                   tcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcg    >  1:3178585/1‑71 (MQ=255)
                                                                    cgcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCGACCATCCCCATGCCGACGCTATTc         >  1:5203480/1‑65 (MQ=255)
                                                                    cgcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGa                 >  1:3722708/1‑57 (MQ=255)
                                                                    cgcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGa                 >  1:2711969/1‑57 (MQ=255)
                                                                    cgcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGa                 >  1:2050557/1‑57 (MQ=255)
                                                                    cgcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAtt          >  1:1287173/1‑64 (MQ=255)
                                                                    cgcgATACATGACATCCCTTATCTGATTGCAAAAAAGCGCAACCATCCCCATGCCGa                 >  1:1172349/1‑57 (MQ=255)
                                                                     gcgATACATGACATCCCTTATCTGATTGCCAAAAAGAGCAACTATCCCCATGCCGACGCTATTCATAcggc  >  1:2421094/1‑71 (MQ=255)
                                                                      |                                                                     
ATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGC  >  Exported/2569731‑2569870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: