Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,569,803 A→T 8.4% I25L (ATA→TTA)  yibI → predicted inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,569,8030AT 8.4% 398.6 / 23.3 142I25L (ATA→TTA) yibIpredicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (65/65);  new base T (9/3);  total (74/68)
Fisher's exact test for biased strand distribution p-value = 1.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

Only 100 of 150 total aligned reads displayed.

GTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGG  >  Exported/2569733‑2569872
                                                                      |                                                                     
gTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa                                                                       >  1:1796788/1‑71 (MQ=255)
  ttCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa                                                                       >  1:1112452/1‑69 (MQ=255)
  ttCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa                                                                       >  1:724577/1‑69 (MQ=255)
   tCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATa                                                                     <  1:5518815/70‑1 (MQ=255)
    cTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACa                                                                   >  1:5613240/1‑71 (MQ=255)
     tAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACAt                                                                  >  1:1824320/1‑71 (MQ=255)
       aaCTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGTTGATTTTTTATGGAATCATCGCGATACATGa                                                                <  1:4099609/71‑1 (MQ=255)
       aaCTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACAt                                                                  <  1:1428015/69‑1 (MQ=255)
       aaCTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACAt                                                                  <  1:3771123/69‑1 (MQ=255)
       aaCTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGa                                                                <  1:5066146/71‑1 (MQ=255)
              tCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCt                                                         >  1:320574/1‑71 (MQ=255)
               cgcgTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCt                                                         >  1:1312826/1‑70 (MQ=255)
                gcgTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCtt                                                        <  1:1162472/70‑1 (MQ=255)
                  gTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATc                                                     >  1:1804155/1‑71 (MQ=255)
                   ttGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt                                                    <  1:2252363/71‑1 (MQ=255)
                    tGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATAc                                                                    >  1:4174044/1‑54 (MQ=255)
                    tGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGa                                                                >  1:58755/1‑58 (MQ=255)
                      ggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGa                                                  <  1:141501/70‑1 (MQ=255)
                      ggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGa                                                  <  1:5462382/70‑1 (MQ=255)
                         gTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa                                                                       >  1:696116/1‑46 (MQ=255)
                         gTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGc                                              <  1:812098/71‑1 (MQ=255)
                          tGCTTATTTTTGTATTTCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGc                                              >  1:4424675/1‑70 (MQ=255)
                          tGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGAt                                                                      <  1:3711333/46‑1 (MQ=255)
                          tGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGAt                                                                      <  1:3392378/46‑1 (MQ=255)
                          tGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGAt                                                                      <  1:2991637/46‑1 (MQ=255)
                          tGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt                                                    <  1:4820180/64‑1 (MQ=255)
                           gCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGa                                                                <  1:2854505/51‑1 (MQ=255)
                           gCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt                                                    <  1:1895950/63‑1 (MQ=255)
                            cTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaa                                           <  1:502164/71‑1 (MQ=255)
                             tGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACa                                                              >  1:743848/1‑51 (MQ=255)
                             tGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACa                                                              >  1:815750/1‑51 (MQ=255)
                             tGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGc                                              <  1:1624129/67‑1 (MQ=255)
                             tGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGc                                              <  1:777398/67‑1 (MQ=255)
                               aTCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTAGCTGATTGCCaaa                                          >  1:5178328/1‑69 (MQ=255)
                                tCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                                       >  1:4984488/1‑71 (MQ=255)
                                tCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                                       >  1:3244098/1‑71 (MQ=255)
                                 cTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGAt                                                 >  1:865341/1‑60 (MQ=255)
                                 cTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGAt                                                 >  1:1338825/1‑60 (MQ=255)
                                   tCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa                                                                       >  1:3710906/1‑36 (MQ=255)
                                    cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa                                                                       >  1:2987201/1‑35 (MQ=255)
                                    cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaaa                                        <  1:2519598/66‑1 (MQ=255)
                                    cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgcgc                                    <  1:467712/70‑1 (MQ=255)
                                     gtatttCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTaa                                  >  1:1521763/4‑71 (MQ=255)
                                     gTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCa                                   <  1:5359199/70‑1 (MQ=255)
                                     gTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCa                                   <  1:789457/70‑1 (MQ=255)
                                      tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgcgc                                    <  1:4690396/68‑1 (MQ=255)
                                      tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAc                                 >  1:2122634/1‑71 (MQ=255)
                                       cTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAc                                 >  1:1727243/1‑70 (MQ=255)
                                       atttCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTaat                                 >  1:253195/2‑69 (MQ=255)
                                        tttCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCa                                   <  1:4724949/67‑1 (MQ=255)
                                        tttCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCa                               >  1:2630246/1‑71 (MQ=255)
                                        tttCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCa                               >  1:2794752/1‑71 (MQ=255)
                                        tttCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTAATCa                               >  1:4881032/1‑71 (MQ=255)
                                          tCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCa                               <  1:2648889/69‑1 (MQ=255)
                                          tCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATc                             >  1:585691/1‑71 (MQ=255)
                                          tCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTAatcatc                             >  1:2103782/1‑71 (MQ=255)
                                          tCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTAatcatc                             >  1:4578061/1‑71 (MQ=255)
                                              gTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcccc                          <  1:4729617/70‑1 (MQ=255)
                                              gTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcccc                          <  1:2434928/70‑1 (MQ=255)
                                              gTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATccc                           <  1:2765947/69‑1 (MQ=255)
                                              gTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTAATCATCCTCa                         <  1:3876165/71‑1 (MQ=255)
                                              gTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTAATCATCCTCa                         <  1:1255475/71‑1 (MQ=255)
                                               tGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCAt                        >  1:4860180/1‑71 (MQ=255)
                                               tGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCAt                        >  1:5042565/1‑71 (MQ=255)
                                               tGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCAt                        >  1:2705846/1‑71 (MQ=255)
                                                 aTTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATg                       <  1:5529270/70‑1 (MQ=255)
                                                 aTTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATg                       <  1:4031945/70‑1 (MQ=255)
                                                   tttttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaa                                         <  1:1553245/50‑1 (MQ=255)
                                                   tttttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaa                                         <  1:2319478/50‑1 (MQ=255)
                                                    ttttATGGAATCATCGCGATACATGACATCCCTTATCTGa                                                  >  1:1020948/1‑40 (MQ=255)
                                                    ttttATGGAATCATCGCGATACATGACATCCCTTATCTGa                                                  >  1:1130441/1‑40 (MQ=255)
                                                    ttttATGGAATCATCGCGATACATGACATCCCTTATCTGa                                                  >  1:2328510/1‑40 (MQ=255)
                                                    ttttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                                       >  1:190146/1‑51 (MQ=255)
                                                     tttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                                       >  1:224250/1‑50 (MQ=255)
                                                     tttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                                       >  1:3969232/1‑50 (MQ=255)
                                                     tttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcc                            >  1:3092708/1‑61 (MQ=255)
                                                      ttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                                       >  1:3549900/1‑49 (MQ=255)
                                                      ttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                                       >  1:2437681/1‑49 (MQ=255)
                                                      ttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACg                 >  1:1469594/1‑71 (MQ=255)
                                                      ttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACg                 >  1:797799/1‑71 (MQ=255)
                                                         tGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa              <  1:2605352/71‑1 (MQ=255)
                                                           gAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                                       >  1:4703683/1‑44 (MQ=255)
                                                            aaTCATCGCGATACATGACATCCCTTATCTGATTGCCaaaaa                                        >  1:4549641/1‑42 (MQ=255)
                                                            aaTCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGcc                     <  1:1603628/61‑1 (MQ=255)
                                                              tcatcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAt             <  1:2111433/67‑1 (MQ=255)
                                                              tcatcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAt             <  1:3408617/67‑1 (MQ=255)
                                                              tcatcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCa          >  1:3992792/1‑70 (MQ=255)
                                                                atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                                       >  1:5237444/1‑39 (MQ=255)
                                                                atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                                       >  1:3527576/1‑39 (MQ=255)
                                                                atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCa          <  1:3140025/68‑1 (MQ=255)
                                                                atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATa        <  1:1477907/70‑1 (MQ=255)
                                                                atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATa        >  1:852705/1‑70 (MQ=255)
                                                                 tcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCaa                                  <  1:5087400/43‑1 (MQ=255)
                                                                 tcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACg                 >  1:1004806/1‑60 (MQ=255)
                                                                  cgcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCGACCATCCCCATGCCGACGCTATTc           >  1:5203480/1‑65 (MQ=255)
                                                                  cgcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGa                   >  1:2711969/1‑57 (MQ=255)
                                                                  cgcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGa                   >  1:2050557/1‑57 (MQ=255)
                                                                  cgcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAtt            >  1:1287173/1‑64 (MQ=255)
                                                                  cgcgATACATGACATCCCTTATCTGATTGCAAAAAAGCGCAACCATCCCCATGCCGa                   >  1:1172349/1‑57 (MQ=255)
                                                                     gATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggcgg  <  1:4708106/71‑1 (MQ=255)
                                                                      |                                                                     
GTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGG  >  Exported/2569733‑2569872

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: