Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,569,811 C→T 10.9% D27D (GAC→GAT yibI → predicted inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,569,8110CT10.9% 398.3 / 28.0 147D27D (GAC→GATyibIpredicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (65/66);  new base T (9/7);  total (74/73)
Fisher's exact test for biased strand distribution p-value = 7.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.88e-01

Only 100 of 147 total aligned reads displayed.

TCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGG  >  Exported/2569747‑2569878
                                                                |                                                                   
tCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCt                                                               >  1:320574/1‑71 (MQ=255)
 cgcgTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCt                                                               >  1:2503064/1‑70 (MQ=255)
    gTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATc                                                           >  1:1804155/1‑71 (MQ=255)
     ttGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt                                                          <  1:2252363/71‑1 (MQ=255)
       gggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTg                                                         <  1:552288/70‑1 (MQ=255)
        ggAGTGCTTATTTTTGTATTTCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGAt                                                       >  1:4515618/1‑71 (MQ=255)
        ggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGa                                                        <  1:5462382/70‑1 (MQ=255)
            tGCTTATTTTTGTATTTCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGc                                                    >  1:4424675/1‑70 (MQ=255)
            tGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt                                                          <  1:4820180/64‑1 (MQ=255)
             gCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt                                                          <  1:2916058/63‑1 (MQ=255)
              cTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCtt                                                              <  1:4868967/58‑1 (MQ=255)
              cTGATCTTCGTCTCTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCa                                                  >  1:5255712/1‑70 (MQ=255)
               tGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACa                                                                    >  1:743848/1‑51 (MQ=255)
               tGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACa                                                                    >  1:815750/1‑51 (MQ=255)
               tGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGc                                                    <  1:1624129/67‑1 (MQ=255)
                 aTCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaa                                                <  1:806317/69‑1 (MQ=255)
                 aTCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTAGCTGATTGCCaaa                                                >  1:5178328/1‑69 (MQ=255)
                  tCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                                             >  1:3244098/1‑71 (MQ=255)
                   cTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGAt                                                       >  1:865341/1‑60 (MQ=255)
                   cTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGAt                                                       >  1:1338825/1‑60 (MQ=255)
                      cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaaa                                              <  1:2519598/66‑1 (MQ=255)
                      cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaaa                                              <  1:5167353/66‑1 (MQ=255)
                      cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaaa                                              <  1:1358557/66‑1 (MQ=255)
                      cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgcgc                                          <  1:467712/70‑1 (MQ=255)
                       gtatttCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTaa                                        >  1:1521763/4‑71 (MQ=255)
                       gTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCa                                         <  1:5359199/70‑1 (MQ=255)
                       gTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCa                                         <  1:789457/70‑1 (MQ=255)
                        tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATccc                                                                <  1:4018103/46‑1 (MQ=255)
                        tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt                                                          <  1:1982204/52‑1 (MQ=255)
                        tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt                                                          <  1:4594536/52‑1 (MQ=255)
                        tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt                                                          <  1:1449400/52‑1 (MQ=255)
                        tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgcgc                                          <  1:4991647/68‑1 (MQ=255)
                        tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgcgc                                          <  1:4690396/68‑1 (MQ=255)
                        tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgcgc                                          <  1:735993/68‑1 (MQ=255)
                        tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAc                                       >  1:2122634/1‑71 (MQ=255)
                        tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAc                                       >  1:782962/1‑71 (MQ=255)
                         cTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAc                                       >  1:1727243/1‑70 (MQ=255)
                          tttCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCa                                         <  1:4724949/67‑1 (MQ=255)
                          tttCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCa                                     >  1:2630246/1‑71 (MQ=255)
                          tttCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCa                                     >  1:2794752/1‑71 (MQ=255)
                          tttCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTAATCa                                     >  1:4881032/1‑71 (MQ=255)
                            tCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAcc                                      <  1:3938435/68‑1 (MQ=255)
                            tCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATc                                   >  1:585691/1‑71 (MQ=255)
                            tCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCaaaaaa                                             >  1:1709133/1‑60 (MQ=255)
                            tCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTaa                                        >  1:1493973/1‑66 (MQ=255)
                            tCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTAatcatc                                   >  1:2103782/1‑71 (MQ=255)
                                gTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcccc                                <  1:4729617/70‑1 (MQ=255)
                                gTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcccc                                <  1:2434928/70‑1 (MQ=255)
                                gTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATccc                                 <  1:2765947/69‑1 (MQ=255)
                                 tGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCAt                              >  1:2705846/1‑71 (MQ=255)
                                 tGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCAt                              >  1:5042565/1‑71 (MQ=255)
                                   aTTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaa                                               >  1:4173963/1‑52 (MQ=255)
                                     tttttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaa                                               <  1:2319478/50‑1 (MQ=255)
                                      ttttATGGAATCATCGCGATACATGACATCCCTTATCTGa                                                        >  1:2328510/1‑40 (MQ=255)
                                      ttttATGGAATCATCGCGATACATGACATCCCTTATCTGa                                                        >  1:1020948/1‑40 (MQ=255)
                                      ttttATGGAATCATCGCGATACATGACATCCCTTATCTGa                                                        >  1:1130441/1‑40 (MQ=255)
                                      ttttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                                             >  1:190146/1‑51 (MQ=255)
                                       tttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                                             >  1:224250/1‑50 (MQ=255)
                                       tttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcc                                  >  1:3497020/1‑61 (MQ=255)
                                       tttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcc                                  >  1:3092708/1‑61 (MQ=255)
                                        ttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCg                          >  1:114063/1‑68 (MQ=255)
                                        ttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGAc                        >  1:5043405/1‑70 (MQ=255)
                                        ttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACg                       >  1:1469594/1‑71 (MQ=255)
                                           tGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa                    <  1:2605352/71‑1 (MQ=255)
                                             gAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                                             >  1:4703683/1‑44 (MQ=255)
                                             gAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                                             >  1:2644590/1‑44 (MQ=255)
                                             gAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAtt                  >  1:2599164/1‑71 (MQ=255)
                                              aaTCATCGCGATACATGACATCCCTTATCTGATTGCCaaaaa                                              >  1:4549641/1‑42 (MQ=255)
                                              aaTCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGcc                           <  1:1603628/61‑1 (MQ=255)
                                                tcatcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAt                   <  1:2111433/67‑1 (MQ=255)
                                                tcatcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAt                   <  1:3408617/67‑1 (MQ=255)
                                                tcatcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCa                >  1:3992792/1‑70 (MQ=255)
                                                tcatcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCAt               <  1:1797360/71‑1 (MQ=255)
                                                  atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                                             >  1:3527576/1‑39 (MQ=255)
                                                  atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                                             >  1:5237444/1‑39 (MQ=255)
                                                  atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATa              >  1:852705/1‑70 (MQ=255)
                                                  atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATa              >  1:1243472/1‑70 (MQ=255)
                                                   tcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACg                       >  1:1004806/1‑60 (MQ=255)
                                                   tcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcg            >  1:3178585/1‑71 (MQ=255)
                                                    cgcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGa                         >  1:2050557/1‑57 (MQ=255)
                                                    cgcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGa                         >  1:3722708/1‑57 (MQ=255)
                                                    cgcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGa                         >  1:2711969/1‑57 (MQ=255)
                                                    cgcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAtt                  >  1:1287173/1‑64 (MQ=255)
                                                    cgcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTc                 >  1:1299216/1‑65 (MQ=255)
                                                    cgcgATACATGACATCCCTTATCTGATTGCAAAAAAGCGCAACCATCCCCATGCCGa                         >  1:1172349/1‑57 (MQ=255)
                                                     gcgATACATGACATCCCTTATCTGATTGCCAAAAAGAGCAACTATCCCCATGCCGACGCTATTCATAcggc          >  1:2421094/1‑71 (MQ=255)
                                                       gATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATccc                                 <  1:576693/46‑1 (MQ=255)
                                                       gATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggcgg        <  1:4708106/71‑1 (MQ=255)
                                                         tACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggc          <  1:3118730/67‑1 (MQ=255)
                                                           cATGATATTCCCTACCTGATTGCCAAAAAACGTAATCATCCTCATGCCGACg                       <  1:1092572/52‑1 (MQ=255)
                                                           cATGATATTCCCTACCTGATTGCCAAAAAACGTAATCATCCTCATGCCGACg                       <  1:811494/52‑1 (MQ=255)
                                                           cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATccc                                 <  1:3715975/42‑1 (MQ=255)
                                                           cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATccc                                 <  1:1594965/42‑1 (MQ=255)
                                                           cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa                    >  1:477828/1‑55 (MQ=255)
                                                           cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggcg         >  1:3457701/1‑66 (MQ=255)
                                                           cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCt     <  1:2381209/70‑1 (MQ=255)
                                                           cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTg    >  1:2695250/1‑71 (MQ=255)
                                                           cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTg    >  1:4990284/1‑71 (MQ=255)
                                                            aTGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTgg   <  1:812775/71‑1 (MQ=255)
                                                             tGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTggg  <  1:3918818/71‑1 (MQ=255)
                                                                |                                                                   
TCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGG  >  Exported/2569747‑2569878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: