Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,569,814 C→T 10.9% I28I (ATC→ATT yibI → predicted inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,569,8140CT10.9% 397.8 / 28.0 147I28I (ATC→ATTyibIpredicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (63/68);  new base T (9/7);  total (72/75)
Fisher's exact test for biased strand distribution p-value = 6.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

Only 100 of 148 total aligned reads displayed.

TCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGC  >  Exported/2569747‑2569883
                                                                   |                                                                     
 cgcgTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCt                                                                    >  1:2503064/1‑70 (MQ=255)
     ttGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt                                                               <  1:2252363/71‑1 (MQ=255)
      tGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt                                                               >  1:644274/1‑70 (MQ=255)
        ggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGa                                                             <  1:5462382/70‑1 (MQ=255)
           gTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGc                                                         <  1:812098/71‑1 (MQ=255)
            tGCTTATTTTTGTATTTCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGc                                                         >  1:4424675/1‑70 (MQ=255)
            tGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt                                                               <  1:4820180/64‑1 (MQ=255)
             gCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt                                                               <  1:1895950/63‑1 (MQ=255)
              cTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCtt                                                                   <  1:4868967/58‑1 (MQ=255)
              cTGATCTTCGTCTCTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCa                                                       >  1:5255712/1‑70 (MQ=255)
               tGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGc                                                         <  1:777398/67‑1 (MQ=255)
                 aTCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaa                                                     <  1:806317/69‑1 (MQ=255)
                 aTCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTAGCTGATTGCCaaa                                                     >  1:5178328/1‑69 (MQ=255)
                  tCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                                                  >  1:3244098/1‑71 (MQ=255)
                   cTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGAt                                                            >  1:865341/1‑60 (MQ=255)
                   cTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGAt                                                            >  1:1338825/1‑60 (MQ=255)
                      cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaaa                                                   <  1:1358557/66‑1 (MQ=255)
                      cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaaa                                                   <  1:5167353/66‑1 (MQ=255)
                      cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgcgc                                               <  1:467712/70‑1 (MQ=255)
                      cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgcgc                                               >  1:3327599/1‑70 (MQ=255)
                       gtatttCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTaa                                             >  1:1521763/4‑71 (MQ=255)
                        tatttCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTaa                                             <  1:27112/68‑1 (MQ=255)
                        tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt                                                               <  1:1449400/52‑1 (MQ=255)
                        tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgcgc                                               <  1:4690396/68‑1 (MQ=255)
                        tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgcgc                                               <  1:4991647/68‑1 (MQ=255)
                        tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAc                                            >  1:782962/1‑71 (MQ=255)
                        tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAc                                            >  1:2122634/1‑71 (MQ=255)
                         atttCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTaat                                            >  1:253195/2‑69 (MQ=255)
                          tttCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCa                                              <  1:4724949/67‑1 (MQ=255)
                          tttCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCa                                          >  1:2630246/1‑71 (MQ=255)
                          tttCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCa                                          >  1:2794752/1‑71 (MQ=255)
                          tttCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTAATCa                                          >  1:4881032/1‑71 (MQ=255)
                            tCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCa                                          <  1:2648889/69‑1 (MQ=255)
                            tCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCaaaaaa                                                  >  1:1709133/1‑60 (MQ=255)
                            tCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTaa                                             >  1:1493973/1‑66 (MQ=255)
                            tCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTAatcatc                                        >  1:4578061/1‑71 (MQ=255)
                                gTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcccc                                     <  1:4729617/70‑1 (MQ=255)
                                gTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTAATCATCCTCa                                    <  1:3876165/71‑1 (MQ=255)
                                 tGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCAt                                   >  1:5042565/1‑71 (MQ=255)
                                   aTTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaa                                                    >  1:4173963/1‑52 (MQ=255)
                                   aTTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATg                                  <  1:4031945/70‑1 (MQ=255)
                                   aTTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATg                                  <  1:5529270/70‑1 (MQ=255)
                                     tttttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaa                                                    <  1:2319478/50‑1 (MQ=255)
                                     tttttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaa                                                    <  1:2109396/50‑1 (MQ=255)
                                      ttttATGGAATCATCGCGATACATGACATCCCTTATCTGa                                                             >  1:1020948/1‑40 (MQ=255)
                                      ttttATGGAATCATCGCGATACATGACATCCCTTATCTGa                                                             >  1:2328510/1‑40 (MQ=255)
                                      ttttATGGAATCATCGCGATACATGACATCCCTTATCTGa                                                             >  1:1130441/1‑40 (MQ=255)
                                      ttttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                                                  >  1:190146/1‑51 (MQ=255)
                                       tttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                                                  >  1:224250/1‑50 (MQ=255)
                                       tttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                                                  >  1:3969232/1‑50 (MQ=255)
                                       tttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcc                                       >  1:3092708/1‑61 (MQ=255)
                                       tttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcc                                       >  1:5449866/1‑61 (MQ=255)
                                       tttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcc                                       >  1:3497020/1‑61 (MQ=255)
                                        ttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                                                  >  1:2437681/1‑49 (MQ=255)
                                        ttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                                                  >  1:3549900/1‑49 (MQ=255)
                                        ttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCg                               >  1:114063/1‑68 (MQ=255)
                                        ttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGAc                             >  1:5043405/1‑70 (MQ=255)
                                        ttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACg                            >  1:797799/1‑71 (MQ=255)
                                        ttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACg                            >  1:1469594/1‑71 (MQ=255)
                                          aTGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGc                           >  1:3002362/1‑70 (MQ=255)
                                             gAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                                                  >  1:4703683/1‑44 (MQ=255)
                                             gAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAtt                       >  1:2599164/1‑71 (MQ=255)
                                              aaTCATCGCGATACATGACATCCCTTATCTGATTGCCaaaaa                                                   >  1:4549641/1‑42 (MQ=255)
                                              aaTCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGcc                                <  1:1603628/61‑1 (MQ=255)
                                                tcatcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAt                        <  1:2111433/67‑1 (MQ=255)
                                                tcatcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAt                        <  1:3408617/67‑1 (MQ=255)
                                                tcatcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCa                     >  1:3992792/1‑70 (MQ=255)
                                                tcatcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCAt                    <  1:1797360/71‑1 (MQ=255)
                                                  atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                                                  >  1:5237444/1‑39 (MQ=255)
                                                  atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAt                        <  1:2776419/65‑1 (MQ=255)
                                                  atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCa                     <  1:3140025/68‑1 (MQ=255)
                                                  atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATa                   >  1:852705/1‑70 (MQ=255)
                                                  atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATa                   >  1:1243472/1‑70 (MQ=255)
                                                   tcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACg                            >  1:1004806/1‑60 (MQ=255)
                                                   tcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcg                 >  1:3178585/1‑71 (MQ=255)
                                                    cgcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGa                              >  1:2711969/1‑57 (MQ=255)
                                                    cgcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGa                              >  1:2050557/1‑57 (MQ=255)
                                                    cgcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAtt                       >  1:1287173/1‑64 (MQ=255)
                                                    cgcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTc                      >  1:1299216/1‑65 (MQ=255)
                                                    cgcgATACATGACATCCCTTATCTGATTGCAAAAAAGCGCAACCATCCCCATGCCGa                              >  1:1172349/1‑57 (MQ=255)
                                                     gcgATACATGACATCCCTTATCTGATTGCCAAAAAGAGCAACTATCCCCATGCCGACGCTATTCATAcggc               >  1:2421094/1‑71 (MQ=255)
                                                       gATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATccc                                      <  1:576693/46‑1 (MQ=255)
                                                       gATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggcgg             <  1:4708106/71‑1 (MQ=255)
                                                         tACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggc               <  1:2005412/67‑1 (MQ=255)
                                                         tACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggc               <  1:1745259/67‑1 (MQ=255)
                                                           cATGATATTCCCTACCTGATTGCCAAAAAACGTAATCATCCTCATGCCGACg                            <  1:1092572/52‑1 (MQ=255)
                                                           cATGACGTCCCTTATCTGATTGCCAAAAAGCGCAACCATcc                                       <  1:3461658/41‑1 (MQ=255)
                                                           cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATccc                                      <  1:3715975/42‑1 (MQ=255)
                                                           cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATccc                                      <  1:1594965/42‑1 (MQ=255)
                                                           cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa                         >  1:477828/1‑55 (MQ=255)
                                                           cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa                         >  1:1651559/1‑55 (MQ=255)
                                                           cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCt          <  1:2381209/70‑1 (MQ=255)
                                                           cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTg         >  1:4990284/1‑71 (MQ=255)
                                                           cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTg         >  1:2695250/1‑71 (MQ=255)
                                                            aTGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGcc                                <  1:2625883/47‑1 (MQ=255)
                                                            aTGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGcc                                <  1:4485198/47‑1 (MQ=255)
                                                            aTGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTgg        <  1:812775/71‑1 (MQ=255)
                                                             tGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTggg       <  1:3918818/71‑1 (MQ=255)
                                                                 aTCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa                         <  1:4898262/49‑1 (MQ=255)
                                                                  tCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa                         <  1:700276/48‑1 (MQ=255)
                                                                   |                                                                     
TCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGC  >  Exported/2569747‑2569883

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: