Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,569,817 T→C 10.6% P29P (CCT→CCC yibI → predicted inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,569,8170TC10.6% 402.6 / 28.5 151P29P (CCT→CCCyibIpredicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (66/69);  new base C (9/7);  total (75/76)
Fisher's exact test for biased strand distribution p-value = 6.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 159 total aligned reads displayed.

TCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGT  >  Exported/2569747‑2569887
                                                                      |                                                                      
 cgcgTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCt                                                                        >  1:1312826/1‑70 (MQ=255)
  gcgTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCtt                                                                       <  1:1162472/70‑1 (MQ=255)
     ttGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt                                                                   <  1:2252363/71‑1 (MQ=255)
      tGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt                                                                   >  1:644274/1‑70 (MQ=255)
       gggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTg                                                                  <  1:552288/70‑1 (MQ=255)
        ggAGTGCTTATTTTTGTATTTCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGAt                                                                >  1:4515618/1‑71 (MQ=255)
        ggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGa                                                                 <  1:222583/70‑1 (MQ=255)
        ggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGa                                                                 <  1:5462382/70‑1 (MQ=255)
        ggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGa                                                                 <  1:141501/70‑1 (MQ=255)
            tGCTTATTTTTGTATTTCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGc                                                             >  1:4424675/1‑70 (MQ=255)
            tGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt                                                                   <  1:4820180/64‑1 (MQ=255)
             gCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt                                                                   <  1:1895950/63‑1 (MQ=255)
              cTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaa                                                          <  1:502164/71‑1 (MQ=255)
              cTGATCTTCGTCTCTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCa                                                           >  1:5255712/1‑70 (MQ=255)
               tGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGc                                                             <  1:777398/67‑1 (MQ=255)
                 aTCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaa                                                         <  1:806317/69‑1 (MQ=255)
                  tCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                                                      >  1:3244098/1‑71 (MQ=255)
                  tCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                                                      >  1:4984488/1‑71 (MQ=255)
                   cTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGAt                                                                >  1:865341/1‑60 (MQ=255)
                   cTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGAt                                                                >  1:1338825/1‑60 (MQ=255)
                      cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaaa                                                       <  1:5167353/66‑1 (MQ=255)
                      cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaaa                                                       <  1:2519598/66‑1 (MQ=255)
                      cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgcgc                                                   >  1:3327599/1‑70 (MQ=255)
                      cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgcgc                                                   <  1:467712/70‑1 (MQ=255)
                       gtatttCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTaa                                                 >  1:1521763/4‑71 (MQ=255)
                       gTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCa                                                  <  1:789457/70‑1 (MQ=255)
                       gTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCa                                                  <  1:5359199/70‑1 (MQ=255)
                        tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgcgc                                                   <  1:4690396/68‑1 (MQ=255)
                        tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgcgc                                                   <  1:735993/68‑1 (MQ=255)
                        tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAc                                                >  1:2122634/1‑71 (MQ=255)
                        tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAc                                                <  1:851204/71‑1 (MQ=255)
                         atttCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTaat                                                >  1:253195/2‑69 (MQ=255)
                          tttCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCa                                              >  1:2794752/1‑71 (MQ=255)
                          tttCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCa                                              >  1:2630246/1‑71 (MQ=255)
                          tttCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTAATCa                                              >  1:4881032/1‑71 (MQ=255)
                            tCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAcc                                               <  1:3938435/68‑1 (MQ=255)
                            tCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAcc                                               <  1:4721837/68‑1 (MQ=255)
                            tCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCaaaaaa                                                      >  1:1709133/1‑60 (MQ=255)
                            tCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTAatcatc                                            >  1:2103782/1‑71 (MQ=255)
                            tCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTAatcatc                                            >  1:4578061/1‑71 (MQ=255)
                                gTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcccc                                         <  1:4729617/70‑1 (MQ=255)
                                gTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcccc                                         <  1:2434928/70‑1 (MQ=255)
                                gTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATccc                                          <  1:2765947/69‑1 (MQ=255)
                                gTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTAATCATCCTCa                                        <  1:3876165/71‑1 (MQ=255)
                                 tGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCAt                                       >  1:5042565/1‑71 (MQ=255)
                                 tGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCAt                                       >  1:2705846/1‑71 (MQ=255)
                                 tGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCAt                                       >  1:4860180/1‑71 (MQ=255)
                                   aTTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATg                                      <  1:4031945/70‑1 (MQ=255)
                                     tttttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaa                                                        <  1:2109396/50‑1 (MQ=255)
                                     tttttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaa                                                        <  1:1553245/50‑1 (MQ=255)
                                      ttttATGGAATCATCGCGATACATGACATCCCTTATCTGa                                                                 >  1:1020948/1‑40 (MQ=255)
                                      ttttATGGAATCATCGCGATACATGACATCCCTTATCTGa                                                                 >  1:1130441/1‑40 (MQ=255)
                                      ttttATGGAATCATCGCGATACATGACATCCCTTATCTGa                                                                 >  1:2328510/1‑40 (MQ=255)
                                      ttttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                                                      >  1:190146/1‑51 (MQ=255)
                                       tttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                                                      >  1:3969232/1‑50 (MQ=255)
                                       tttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcc                                           >  1:3497020/1‑61 (MQ=255)
                                        ttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                                                      >  1:3549900/1‑49 (MQ=255)
                                        ttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                                                      >  1:2437681/1‑49 (MQ=255)
                                        ttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACg                                >  1:1469594/1‑71 (MQ=255)
                                          aTGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGc                               >  1:3002362/1‑70 (MQ=255)
                                           tGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa                             <  1:2605352/71‑1 (MQ=255)
                                             gAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                                                      >  1:2644590/1‑44 (MQ=255)
                                             gAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                                                      >  1:4703683/1‑44 (MQ=255)
                                              aaTCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGcc                                    <  1:1603628/61‑1 (MQ=255)
                                              aaTCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGcc                                    <  1:4775917/61‑1 (MQ=255)
                                                tcatcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAt                            <  1:3408617/67‑1 (MQ=255)
                                                tcatcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCAt                        <  1:1797360/71‑1 (MQ=255)
                                                  atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATa                       <  1:1477907/70‑1 (MQ=255)
                                                  atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATa                       >  1:1243472/1‑70 (MQ=255)
                                                   tcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCaa                                                 <  1:5087400/43‑1 (MQ=255)
                                                   tcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACg                                >  1:1004806/1‑60 (MQ=255)
                                                   tcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcg                     >  1:3178585/1‑71 (MQ=255)
                                                    cgcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCGACCATCCCCATGCCGACGCTATTc                          >  1:5203480/1‑65 (MQ=255)
                                                    cgcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGa                                  >  1:3722708/1‑57 (MQ=255)
                                                    cgcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGa                                  >  1:2711969/1‑57 (MQ=255)
                                                    cgcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAtt                           >  1:1287173/1‑64 (MQ=255)
                                                       gATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATccc                                          <  1:576693/46‑1 (MQ=255)
                                                         tACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggc                   <  1:2005412/67‑1 (MQ=255)
                                                         tACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggc                   <  1:3118730/67‑1 (MQ=255)
                                                         tACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggc                   <  1:1745259/67‑1 (MQ=255)
                                                           cATGATATTCCCTACCTGATTGCCAAAAAACGTAATCATCCTCATGCCGACg                                <  1:1127274/52‑1 (MQ=255)
                                                           cATGATATTCCCTACCTGATTGCCAAAAAACGTAATCATCCTCATGCCGACg                                <  1:811494/52‑1 (MQ=255)
                                                           cATGATATTCCCTACCTGATTGCCAAAAAACGTAATCATCCTCATGCCGACg                                <  1:2316378/52‑1 (MQ=255)
                                                           cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa                             >  1:1651559/1‑55 (MQ=255)
                                                           cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggcg                  >  1:3457701/1‑66 (MQ=255)
                                                           cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCt              <  1:2381209/70‑1 (MQ=255)
                                                           cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTg             >  1:4990284/1‑71 (MQ=255)
                                                           cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTg             >  1:2695250/1‑71 (MQ=255)
                                                            aTGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGcc                                    <  1:4485198/47‑1 (MQ=255)
                                                            aTGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTgg            <  1:812775/71‑1 (MQ=255)
                                                                  tCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGc      <  1:4346774/71‑1 (MQ=255)
                                                                    ccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATg                                      <  1:1170258/37‑1 (MQ=255)
                                                                    ccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGa                                  >  1:2946378/1‑41 (MQ=255)
                                                                    ccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGa                                  >  1:3127835/1‑41 (MQ=255)
                                                                    ccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCAt                        >  1:4697492/1‑51 (MQ=255)
                                                                    ccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGc      <  1:3576495/69‑1 (MQ=255)
                                                                    ccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCt    >  1:2166381/1‑71 (MQ=255)
                                                                    ccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCt    <  1:2332947/71‑1 (MQ=255)
                                                                      ttATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa                             >  1:649719/1‑44 (MQ=255)
                                                                      ttATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa                             >  1:1282397/1‑44 (MQ=255)
                                                                      |                                                                      
TCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGT  >  Exported/2569747‑2569887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: