Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,569,838 C→T 18.6% R36R (CGC→CGT yibI → predicted inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,569,8380CT18.6% 365.5 / 66.7 166R36R (CGC→CGTyibIpredicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (79/56);  new base T (20/11);  total (99/67)
Fisher's exact test for biased strand distribution p-value = 6.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.63e-01

Only 100 of 174 total aligned reads displayed.

CGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTG  >  Exported/2569769‑2569906
                                                                     |                                                                    
cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgcgc                                                                      <  1:467712/70‑1 (MQ=255)
  tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgcgc                                                                      <  1:4991647/68‑1 (MQ=255)
  tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAc                                                                   >  1:782962/1‑71 (MQ=255)
   atttCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTaat                                                                   >  1:253195/2‑69 (MQ=255)
    tttCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCa                                                                     <  1:4724949/67‑1 (MQ=255)
      tCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCa                                                                 <  1:2648889/69‑1 (MQ=255)
      tCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATc                                                               >  1:585691/1‑71 (MQ=255)
      tCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTaa                                                                    >  1:1493973/1‑66 (MQ=255)
      tCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTAatcatc                                                               >  1:4578061/1‑71 (MQ=255)
      tCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTAatcatc                                                               >  1:2103782/1‑71 (MQ=255)
          gTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTAATCATCCTCa                                                           <  1:3876165/71‑1 (MQ=255)
           tGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCAt                                                          >  1:2705846/1‑71 (MQ=255)
           tGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCAt                                                          >  1:4860180/1‑71 (MQ=255)
                 tttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcc                                                              >  1:3092708/1‑61 (MQ=255)
                 tttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcc                                                              >  1:5449866/1‑61 (MQ=255)
                  ttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGAc                                                    >  1:5043405/1‑70 (MQ=255)
                  ttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACg                                                   >  1:797799/1‑71 (MQ=255)
                    aTGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGc                                                  >  1:3002362/1‑70 (MQ=255)
                       gAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAtt                                              >  1:2599164/1‑71 (MQ=255)
                       gAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAtt                                              >  1:4691944/1‑71 (MQ=255)
                        aaTCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGcc                                                       <  1:4775917/61‑1 (MQ=255)
                          tcatcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAt                                               <  1:2111433/67‑1 (MQ=255)
                          tcatcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCa                                            >  1:3992792/1‑70 (MQ=255)
                            atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAt                                               <  1:2776419/65‑1 (MQ=255)
                            atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCa                                            <  1:3140025/68‑1 (MQ=255)
                            atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATa                                          >  1:1243472/1‑70 (MQ=255)
                            atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATa                                          >  1:852705/1‑70 (MQ=255)
                            atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATa                                          <  1:1477907/70‑1 (MQ=255)
                             tcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCaa                                                                    <  1:5087400/43‑1 (MQ=255)
                             tcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACg                                                   >  1:1004806/1‑60 (MQ=255)
                             tcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcg                                        >  1:3178585/1‑71 (MQ=255)
                              cgcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGa                                                     >  1:2711969/1‑57 (MQ=255)
                              cgcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTc                                             >  1:1299216/1‑65 (MQ=255)
                               gcgATACATGACATCCCTTATCTGATTGCCAAAAAGAGCAACTATCCCCATGCCGACGCTATTCATAcggc                                      >  1:2421094/1‑71 (MQ=255)
                                 gATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggcgg                                    <  1:4708106/71‑1 (MQ=255)
                                   tACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGc                                                  <  1:2350146/55‑1 (MQ=255)
                                   tACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggc                                      <  1:1745259/67‑1 (MQ=255)
                                   tACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggc                                      <  1:3118730/67‑1 (MQ=255)
                                   tACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggc                                      <  1:2005412/67‑1 (MQ=255)
                                     cATGATATTCCCTACCTGATTGCCAAAAAACGTAATCATCCTCATGCCGACg                                                   <  1:1127274/52‑1 (MQ=255)
                                     cATGATATTCCCTACCTGATTGCCAAAAAACGTAATCATCCTCATGCCGACg                                                   <  1:2316378/52‑1 (MQ=255)
                                     cATGACGTCCCTTATCTGATTGCCAAAAAGCGCAACCATcc                                                              <  1:3461658/41‑1 (MQ=255)
                                     cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATccc                                                             <  1:1594965/42‑1 (MQ=255)
                                     cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATccc                                                             <  1:3715975/42‑1 (MQ=255)
                                     cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa                                                >  1:1651559/1‑55 (MQ=255)
                                     cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggcg                                     >  1:3457701/1‑66 (MQ=255)
                                      aTGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGcc                                                       <  1:2625883/47‑1 (MQ=255)
                                      aTGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTgg                               <  1:812775/71‑1 (MQ=255)
                                            tCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGc                         <  1:4346774/71‑1 (MQ=255)
                                              ccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATg                                                         <  1:1170258/37‑1 (MQ=255)
                                              ccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGa                                                     >  1:2946378/1‑41 (MQ=255)
                                              ccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCAt                                           >  1:3402966/1‑51 (MQ=255)
                                              ccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCt                       >  1:2166381/1‑71 (MQ=255)
                                              ccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCt                       <  1:2332947/71‑1 (MQ=255)
                                               cTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTg                      >  1:4444284/1‑71 (MQ=255)
                                                ttATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa                                                >  1:1282397/1‑44 (MQ=255)
                                                ttATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGt                     >  1:2197050/1‑71 (MQ=255)
                                                 tATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGa                                                     >  1:3921082/1‑38 (MQ=255)
                                                 tATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGa                                                     >  1:608827/1‑38 (MQ=255)
                                                 tATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGa                                                     >  1:1926275/1‑38 (MQ=255)
                                                 tATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACg                                                   <  1:2734561/40‑1 (MQ=255)
                                                 tATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTc                                             >  1:4624941/1‑46 (MQ=255)
                                                 tATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTgg                               <  1:3690337/60‑1 (MQ=255)
                                                 tATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGtt                    >  1:3801171/1‑71 (MQ=255)
                                                  accTGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCtttt                    >  1:4720228/3‑70 (MQ=255)
                                                  accTGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCtttt                    >  1:1425786/3‑70 (MQ=255)
                                                  accTGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCtttt                    >  1:5464106/3‑70 (MQ=255)
                                                  aTCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGtt                    <  1:1084463/70‑1 (MQ=255)
                                                  aTCTGATTGCCAAAAAGCGCAACCATCCCAATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGtt                    >  1:1190558/1‑70 (MQ=255)
                                                   tCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcgg                                       >  1:802442/1‑50 (MQ=255)
                                                   tCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGtt                    <  1:3732014/69‑1 (MQ=255)
                                                   ccTGATTGCCAAAAAACGTAATCATCCTCATGCCGa                                                     >  1:831240/2‑36 (MQ=16)
                                                   ccTGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCtttt                    <  1:3537745/68‑1 (MQ=255)
                                                    cTGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTtttt                   <  1:4888679/69‑1 (MQ=255)
                                                     tGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAtt                                              <  1:2790608/41‑1 (MQ=255)
                                                     tGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAtt                                              <  1:4778394/41‑1 (MQ=255)
                                                      gATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTAcgt               >  1:172019/1‑69 (MQ=255)
                                                       aTTGCCAAAAAGCGCAACCATCCTCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTAc                 <  1:5367204/68‑1 (MQ=255)
                                                       aTTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCa                                            >  1:5217061/1‑41 (MQ=19)
                                                        ttGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTActct              >  1:3853554/1‑70 (MQ=255)
                                                        ttGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTg             >  1:1663345/1‑71 (MQ=255)
                                                          gCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTa                  >  1:3065113/1‑64 (MQ=255)
                                                            cAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATg         >  1:4926543/1‑71 (MQ=255)
                                                             aaaaaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGt        >  1:3214145/1‑71 (MQ=255)
                                                             aaaaaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGt        >  1:1269569/1‑71 (MQ=255)
                                                             aaaaaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGt        >  1:660056/1‑71 (MQ=255)
                                                              aaaaGCGCAACCATCCCCATGCCGACGCTATTCAt                                           <  1:2710445/35‑1 (MQ=255)
                                                              aaaaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTa                  >  1:933933/1‑60 (MQ=255)
                                                              aaaaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTa                  >  1:5133009/1‑60 (MQ=255)
                                                              aaaaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGt        >  1:722329/1‑70 (MQ=255)
                                                              aaaaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGt        >  1:435764/1‑70 (MQ=255)
                                                              aaaaGCGCAACCATCCCCAAGCCGACGCTATTCATAcggcgg                                    >  1:2823040/1‑42 (MQ=255)
                                                              aaaaACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGt        >  1:4942626/1‑70 (MQ=255)
                                                                aaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTgg                               >  1:2292439/1‑45 (MQ=255)
                                                                aaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTgg                               >  1:780185/1‑45 (MQ=255)
                                                                aaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTAt     >  1:1535867/1‑71 (MQ=255)
                                                                aaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTAt     >  1:1164799/1‑71 (MQ=255)
                                                                 aGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATc    <  1:5631368/71‑1 (MQ=255)
                                                                 aGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATc    <  1:5297502/71‑1 (MQ=255)
                                                                   cgcAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTg  >  1:4147305/1‑71 (MQ=255)
                                                                     |                                                                    
CGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTG  >  Exported/2569769‑2569906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: