| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 311,787 | G→C | 11.7% | F362L (TTC→TTG) ‡ | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 311,787 | 0 | G | C | 11.7% | 353.0 / 24.8 | 119 | F362L (TTC→TTG) ‡ | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base G (43/62); new base C (7/7); total (50/69) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.72e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 127 total aligned reads displayed.
AGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACT > Exported/311717‑311856 | aGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCgg < 1:3663923/71‑1 (MQ=255) aaGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCt < 1:1388741/71‑1 (MQ=255) aGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTg > 1:2082518/1‑71 (MQ=255) aGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTg > 1:2930044/1‑71 (MQ=255) aGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGGCGGAATCAACGTCGCGCGGAAGTTCTg < 1:1104062/71‑1 (MQ=255) aGGACGGCAAAGGTCCCGAGAAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTg > 1:3748501/1‑71 (MQ=255) aCGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAg > 1:612465/1‑71 (MQ=255) aCGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAg > 1:3481233/1‑71 (MQ=255) ggCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGa < 1:3631523/71‑1 (MQ=255) cAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAAc < 1:5176462/71‑1 (MQ=255) aaGGTCCCCAGCAGCACCACCGGTACGGCGATGGTCGGGATCAGCGTGGCGCGGAAGTTCTGCAGGAACAg > 1:5237249/1‑71 (MQ=11) aaGGTCCCCAGCAGCACCACCGGTACGGCGATGGTCGGGATCAGCGTGGCGCGGAAGTTCTGCAGGAACAg > 1:5215938/1‑71 (MQ=11) aaGGTCCCCAGCAGCACCACCGGTACGGCGATGGTCGGGATCAGCGTGGCGCGGAAGTTCTGCAGGAACAg > 1:2637157/1‑71 (MQ=11) aGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAg < 1:13541/56‑1 (MQ=255) aGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAg < 1:2961535/56‑1 (MQ=255) aGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAg < 1:4773430/56‑1 (MQ=255) aGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAg < 1:2496179/56‑1 (MQ=255) aGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAg < 1:2566969/56‑1 (MQ=255) aGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAg < 1:4473877/56‑1 (MQ=255) ggTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGa > 1:1236659/1‑70 (MQ=255) ccGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGaa > 1:4441074/1‑50 (MQ=255) ccGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACa < 1:122985/64‑1 (MQ=255) ccGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACa < 1:3948426/70‑1 (MQ=255) cGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAcatacata < 1:128063/71‑1 (MQ=255) aacAACACCACGGGTACCGCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCAc > 1:4922309/3‑71 (MQ=2) aGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGaa > 1:2609659/1‑59 (MQ=255) aGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGaa > 1:2727410/1‑59 (MQ=255) aGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGaa > 1:2780819/1‑59 (MQ=255) aGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAAc < 1:966593/71‑1 (MQ=255) gCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAAcc < 1:1008852/71‑1 (MQ=255) acAACACCACGGGTACCGCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCAc > 1:1975921/2‑70 (MQ=2) aacaccacgGGTACCGCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCACCAg > 1:4596896/4‑71 (MQ=2) aaTACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACAt < 1:511256/65‑1 (MQ=255) acaccacgGGTACCGCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCACCAgg < 1:4670260/69‑1 (MQ=9) accacgGGTACCGCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCACCAgg < 1:1106224/69‑1 (MQ=9) accaccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGaa > 1:295208/1‑41 (MQ=255) accaccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGaa > 1:2786853/1‑41 (MQ=255) accaccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGaa > 1:822193/1‑41 (MQ=255) ccaccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGaa > 1:1083101/1‑40 (MQ=255) ccaccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGaa > 1:1165736/1‑40 (MQ=255) ccaccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACAt > 1:4393492/1‑61 (MQ=255) ccaccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGaa > 1:4377344/1‑70 (MQ=255) ccaccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGAAGGAACAg > 1:2907685/1‑55 (MQ=255) caccGGTACGGCAATGGTCGGAATCAACGTCGCGCgg > 1:4014668/1‑37 (MQ=255) caccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAAc < 1:3971975/52‑1 (MQ=255) caccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAAc < 1:5398554/52‑1 (MQ=255) acgggTACCGCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCACCAg > 1:3124164/4‑65 (MQ=2) acgggTACCGCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCACCAGGAacac < 1:5493422/68‑1 (MQ=9) accGGTACGGCAATGGTCGGAATCAACTTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAacac < 1:4566776/71‑1 (MQ=255) accGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAgg < 1:4882378/66‑1 (MQ=255) accGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAacac < 1:3584368/71‑1 (MQ=255) accGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAacac < 1:297754/71‑1 (MQ=255) accGGCACCGCGATCGTCGGGATCAGCGTCGCCCGCCAATTCTGCAGGAACAGGAACATCACCAGGAagac < 1:4785712/71‑1 (MQ=2) ccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAg > 1:266931/1‑52 (MQ=255) ggTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAg > 1:1448485/1‑71 (MQ=255) tACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGga > 1:2878751/1‑71 (MQ=255) ccgCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCACCAGGAACACCAGcat < 1:3775086/68‑1 (MQ=9) cGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGgatg < 1:4147432/71‑1 (MQ=255) gCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAAc > 1:2497879/1‑42 (MQ=255) gCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGgatgat < 1:1848565/71‑1 (MQ=255) cAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCAcc < 1:1768456/53‑1 (MQ=2) aaTGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCACCAGGACCACCAGcataatg > 1:5560352/1‑69 (MQ=2) aTGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGa > 1:1807280/1‑42 (MQ=255) aTGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACAt < 1:1387417/47‑1 (MQ=255) aTGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATa < 1:2646836/48‑1 (MQ=255) tGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGaa > 1:1701577/1‑37 (MQ=255) tGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAgg < 1:1423131/53‑1 (MQ=255) tGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAg < 1:2015780/61‑1 (MQ=255) tGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAg < 1:3283076/61‑1 (MQ=255) tGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAg < 1:1524122/61‑1 (MQ=255) tGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAg < 1:5267957/61‑1 (MQ=255) ggTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAg > 1:4168476/1‑60 (MQ=255) ggTCGGAATCAACGTCGCGAGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCtt > 1:5645169/1‑71 (MQ=255) gTCGGATTCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAg < 1:4376172/59‑1 (MQ=255) cGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGa > 1:1014996/1‑71 (MQ=255) aaTCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCa < 1:5031656/71‑1 (MQ=255) aTCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAg > 1:1071591/1‑71 (MQ=255) aTCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAg > 1:2390574/1‑71 (MQ=255) tCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACg < 1:1713518/50‑1 (MQ=255) tCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGgatg < 1:1918509/56‑1 (MQ=255) tCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGgatg < 1:2094437/56‑1 (MQ=255) cGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAgg < 1:4149973/40‑1 (MQ=255) cGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAgg < 1:4869205/40‑1 (MQ=255) cGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGttt > 1:2748303/1‑71 (MQ=255) cGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGttt > 1:5371946/1‑71 (MQ=255) gTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGtttt < 1:3121986/71‑1 (MQ=255) gTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGtttt < 1:3234591/71‑1 (MQ=255) gTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGtttt < 1:1484380/71‑1 (MQ=255) gTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGtttt < 1:950230/71‑1 (MQ=255) gTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGtttt < 1:4208913/71‑1 (MQ=255) tCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCg < 1:1937636/66‑1 (MQ=255) tCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTa < 1:3918965/71‑1 (MQ=255) tCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTa < 1:1121907/71‑1 (MQ=255) tCGCGCGGAAGTTCTGAAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCg < 1:2248828/66‑1 (MQ=255) gcgcgGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTaa > 1:3370189/1‑70 (MQ=255) gcgcgGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTaa > 1:1197818/1‑70 (MQ=255) gcgcgGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAAc < 1:2932654/71‑1 (MQ=255) cgGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc < 1:4399059/71‑1 (MQ=255) ggAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACt > 1:3698759/1‑71 (MQ=255) gAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAgg < 1:3898201/40‑1 (MQ=255) | AGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACT > Exported/311717‑311856 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |