Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,569,841 C→T 16.2% N37N (AAC→AAT yibI → predicted inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,569,8410CT16.2% 366.4 / 50.8 154N37N (AAC→AATyibIpredicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (77/52);  new base T (15/10);  total (92/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01

Only 100 of 168 total aligned reads displayed.

TCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGT  >  Exported/2569771‑2569911
                                                                      |                                                                      
tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAc                                                                        <  1:851204/71‑1 (MQ=255)
 cTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAc                                                                        >  1:1727243/1‑70 (MQ=255)
  tttCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCa                                                                      >  1:2794752/1‑71 (MQ=255)
  tttCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTAATCa                                                                      >  1:4881032/1‑71 (MQ=255)
    tCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAcc                                                                       <  1:3938435/68‑1 (MQ=255)
    tCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCa                                                                      <  1:2648889/69‑1 (MQ=255)
    tCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATc                                                                    >  1:585691/1‑71 (MQ=255)
        gTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcccc                                                                 <  1:4729617/70‑1 (MQ=255)
        gTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATccc                                                                  <  1:2765947/69‑1 (MQ=255)
        gTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTAATCATCCTCa                                                                <  1:3876165/71‑1 (MQ=255)
        gTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTAATCATCCTCa                                                                <  1:1255475/71‑1 (MQ=255)
         tGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCAt                                                               >  1:2705846/1‑71 (MQ=255)
           aTTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATg                                                              <  1:4031945/70‑1 (MQ=255)
           aTTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATg                                                              <  1:5529270/70‑1 (MQ=255)
               tttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcc                                                                   >  1:5449866/1‑61 (MQ=255)
               tttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcc                                                                   >  1:3092708/1‑61 (MQ=255)
                ttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGAc                                                         >  1:5043405/1‑70 (MQ=255)
                ttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACg                                                        >  1:797799/1‑71 (MQ=255)
                ttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACg                                                        >  1:1469594/1‑71 (MQ=255)
                  aTGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGc                                                       >  1:3002362/1‑70 (MQ=255)
                   tGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa                                                     <  1:2605352/71‑1 (MQ=255)
                     gAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAtt                                                   >  1:4691944/1‑71 (MQ=255)
                      aaTCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGcc                                                            <  1:4775917/61‑1 (MQ=255)
                      aaTCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGcc                                                            <  1:1603628/61‑1 (MQ=255)
                        tcatcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAt                                                    <  1:2111433/67‑1 (MQ=255)
                        tcatcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAt                                                    <  1:3408617/67‑1 (MQ=255)
                        tcatcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAt                                                    <  1:906763/67‑1 (MQ=255)
                        tcatcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCAt                                                <  1:1797360/71‑1 (MQ=255)
                          atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAt                                                    <  1:2776419/65‑1 (MQ=255)
                          atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCa                                                 <  1:3140025/68‑1 (MQ=255)
                          atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATa                                               >  1:1243472/1‑70 (MQ=255)
                           tcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACg                                                        >  1:1004806/1‑60 (MQ=255)
                           tcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcg                                             >  1:3178585/1‑71 (MQ=255)
                            cgcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGa                                                          >  1:2050557/1‑57 (MQ=255)
                            cgcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTc                                                  >  1:1299216/1‑65 (MQ=255)
                             gcgATACATGACATCCCTTATCTGATTGCCAAAAAGAGCAACTATCCCCATGCCGACGCTATTCATAcggc                                           >  1:2421094/1‑71 (MQ=255)
                               gATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATccc                                                                  <  1:576693/46‑1 (MQ=255)
                                 tACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggc                                           <  1:3118730/67‑1 (MQ=255)
                                   cATGATATTCCCTACCTGATTGCCAAAAAACGTAATCATCCTCATGCCGACg                                                        <  1:811494/52‑1 (MQ=255)
                                   cATGATATTCCCTACCTGATTGCCAAAAAACGTAATCATCCTCATGCCGACg                                                        <  1:1127274/52‑1 (MQ=255)
                                   cATGATATTCCCTACCTGATTGCCAAAAAACGTAATCATCCTCATGCCGACg                                                        <  1:2316378/52‑1 (MQ=255)
                                   cATGACGTCCCTTATCTGATTGCCAAAAAGCGCAACCATcc                                                                   <  1:3461658/41‑1 (MQ=255)
                                   cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATccc                                                                  <  1:3715975/42‑1 (MQ=255)
                                   cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggcg                                          >  1:3457701/1‑66 (MQ=255)
                                   cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCt                                      <  1:2381209/70‑1 (MQ=255)
                                   cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTg                                     >  1:4990284/1‑71 (MQ=255)
                                    aTGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGcc                                                            <  1:2625883/47‑1 (MQ=255)
                                    aTGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGcc                                                            <  1:4485198/47‑1 (MQ=255)
                                         aTCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa                                                     <  1:4898262/49‑1 (MQ=255)
                                          tCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa                                                     <  1:700276/48‑1 (MQ=255)
                                          tCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGc                              <  1:4346774/71‑1 (MQ=255)
                                            ccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATg                                                              <  1:1170258/37‑1 (MQ=255)
                                            ccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGa                                                          >  1:2946378/1‑41 (MQ=255)
                                            ccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCAt                                                >  1:4697492/1‑51 (MQ=255)
                                            ccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCt                            >  1:2166381/1‑71 (MQ=255)
                                             cTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTg                           >  1:4444284/1‑71 (MQ=255)
                                              ttATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa                                                     >  1:1282397/1‑44 (MQ=255)
                                              ttATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa                                                     >  1:649719/1‑44 (MQ=255)
                                               tATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGa                                                          >  1:3921082/1‑38 (MQ=255)
                                               tATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGa                                                          >  1:1926275/1‑38 (MQ=255)
                                               tATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTc                                                  >  1:1708686/1‑46 (MQ=255)
                                               tATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTc                                                  >  1:4624941/1‑46 (MQ=255)
                                               tATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTgg                                    <  1:3690337/60‑1 (MQ=255)
                                               tATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGtt                         >  1:3801171/1‑71 (MQ=255)
                                                accTGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCtttt                         >  1:3829765/3‑70 (MQ=255)
                                                accTGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCtttt                         >  1:4720228/3‑70 (MQ=255)
                                                aTCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCa                                                 >  1:1366089/1‑46 (MQ=255)
                                                aTCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGtt                         >  1:5463544/1‑70 (MQ=255)
                                                aTCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGtt                         <  1:1084463/70‑1 (MQ=255)
                                                aTCTGATTGCCAAAAAGCGCAACCATCCCAATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGtt                         >  1:1190558/1‑70 (MQ=255)
                                                 tCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGtt                         <  1:3732014/69‑1 (MQ=255)
                                                 ccTGATTGCCAAAAAACGTAATCATCCTCATGCCGa                                                          >  1:831240/2‑36 (MQ=16)
                                                 ccTGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCtttt                         <  1:3537745/68‑1 (MQ=255)
                                                  cTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTg                           <  1:5044695/66‑1 (MQ=255)
                                                  cTGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCttt                          <  1:2727207/67‑3 (MQ=255)
                                                  cTGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTAc                      <  1:1573822/71‑1 (MQ=255)
                                                   tGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAtt                                                   <  1:4778394/41‑1 (MQ=255)
                                                   tGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAt                                                    >  1:2862651/1‑40 (MQ=255)
                                                   tGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTAct                     >  1:4354769/1‑71 (MQ=255)
                                                     aTTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTActct                   >  1:2202953/1‑71 (MQ=255)
                                                     aTTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCa                                                 >  1:5217061/1‑41 (MQ=19)
                                                      ttGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTg                  <  1:4366441/71‑1 (MQ=255)
                                                      ttGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTg                  >  1:1663345/1‑71 (MQ=255)
                                                       tGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTg                  >  1:992449/1‑70 (MQ=255)
                                                       tGCCAAAAAACGTAATCATCCTCATGCCGACGCCAtt                                                   >  1:2111344/1‑37 (MQ=16)
                                                          cAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATg              >  1:4926543/1‑71 (MQ=255)
                                                           aaaaaaCGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGt             >  1:2334898/1‑71 (MQ=255)
                                                           aaaaaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGt             >  1:3214145/1‑71 (MQ=255)
                                                            aaaaGCGCAACCATCCCCATGCCGACGCTATTCAt                                                <  1:2710445/35‑1 (MQ=255)
                                                              aaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGTCTGGGTGAGCCTGTTTACTCTGCATGTTAt          >  1:308777/1‑71 (MQ=255)
                                                              aaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTgg                                    >  1:780185/1‑45 (MQ=255)
                                                              aaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTAt          >  1:1535867/1‑71 (MQ=255)
                                                              aaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTAt          >  1:1164799/1‑71 (MQ=255)
                                                               aGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATc         <  1:22948/71‑1 (MQ=255)
                                                               aGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATc         <  1:5297502/71‑1 (MQ=255)
                                                                gcgcAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCt        <  1:79918/71‑1 (MQ=255)
                                                                      ccATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGt  <  1:2571623/71‑1 (MQ=255)
                                                                      ccATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGt  <  1:660972/71‑1 (MQ=255)
                                                                      ccATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGt  <  1:5276981/71‑1 (MQ=255)
                                                                      ccATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGt  <  1:515595/71‑1 (MQ=255)
                                                                      |                                                                      
TCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGT  >  Exported/2569771‑2569911

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: