Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,569,859 T→C 27.7% A43A (GCT→GCC yibI → predicted inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,569,8590TC27.7% 238.9 / 108.4 159A43A (GCT→GCCyibIpredicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (57/58);  new base C (24/20);  total (81/78)
Fisher's exact test for biased strand distribution p-value = 5.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 159 total aligned reads displayed.

TGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGG  >  Exported/2569790‑2569926
                                                                     |                                                                   
  gAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAtt                                                                  >  1:2599164/1‑71 (MQ=255)
     tcatcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAt                                                                   <  1:906763/67‑1 (MQ=255)
     tcatcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCa                                                                >  1:3992792/1‑70 (MQ=255)
       atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAt                                                                   <  1:2776419/65‑1 (MQ=255)
       atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATa                                                              <  1:1477907/70‑1 (MQ=255)
       atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATa                                                              >  1:1243472/1‑70 (MQ=255)
        tcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcg                                                            >  1:3178585/1‑71 (MQ=255)
         cgcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCGACCATCCCCATGCCGACGCTATTc                                                                 >  1:5203480/1‑65 (MQ=255)
         cgcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAtt                                                                  >  1:1287173/1‑64 (MQ=255)
          gcgATACATGACATCCCTTATCTGATTGCCAAAAAGAGCAACTATCCCCATGCCGACGCTATTCATAcggc                                                          >  1:2421094/1‑71 (MQ=255)
            gATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggcgg                                                        <  1:4708106/71‑1 (MQ=255)
              tACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggc                                                          <  1:3118730/67‑1 (MQ=255)
              tACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggc                                                          <  1:2005412/67‑1 (MQ=255)
              tACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggc                                                          <  1:1745259/67‑1 (MQ=255)
                cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa                                                                    >  1:477828/1‑55 (MQ=255)
                cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggcg                                                         >  1:3457701/1‑66 (MQ=255)
                cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTg                                                    >  1:2695250/1‑71 (MQ=255)
                 aTGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTgg                                                   <  1:812775/71‑1 (MQ=255)
                  tGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTggg                                                  <  1:3918818/71‑1 (MQ=255)
                      aTCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa                                                                    <  1:4898262/49‑1 (MQ=255)
                       tCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa                                                                    <  1:700276/48‑1 (MQ=255)
                       tCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGc                                             <  1:4346774/71‑1 (MQ=255)
                         ccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCAt                                                               >  1:3402966/1‑51 (MQ=255)
                         ccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCAt                                                               >  1:4697492/1‑51 (MQ=255)
                         ccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGc                                             <  1:3576495/69‑1 (MQ=255)
                         ccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCt                                           >  1:2166381/1‑71 (MQ=255)
                         ccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCt                                           <  1:2332947/71‑1 (MQ=255)
                          cTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTg                                          >  1:4444284/1‑71 (MQ=255)
                           ttATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa                                                                    >  1:1282397/1‑44 (MQ=255)
                           ttATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa                                                                    >  1:649719/1‑44 (MQ=255)
                           ttATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGt                                         >  1:2197050/1‑71 (MQ=255)
                            tATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTc                                                                 >  1:1708686/1‑46 (MQ=255)
                            tATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTc                                                                 >  1:4624941/1‑46 (MQ=255)
                            tATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTgg                                                   <  1:3690337/60‑1 (MQ=255)
                             accTGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCtttt                                        >  1:4671916/3‑70 (MQ=255)
                             accTGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCtttt                                        >  1:5464106/3‑70 (MQ=255)
                             accTGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCtttt                                        >  1:3829765/3‑70 (MQ=255)
                             aTCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa                                                                    >  1:3741527/1‑42 (MQ=255)
                             aTCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGtt                                        >  1:5463544/1‑70 (MQ=255)
                             aTCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGtt                                        <  1:1084463/70‑1 (MQ=255)
                             aTCTGATTGCCAAAAAGCGCAACCATCCCAATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGtt                                        >  1:1190558/1‑70 (MQ=255)
                              tCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcgg                                                           >  1:802442/1‑50 (MQ=255)
                              ccTGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCtttt                                        <  1:3537745/68‑1 (MQ=255)
                               cTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTg                                          <  1:5044695/66‑1 (MQ=255)
                               cTGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCttt                                         <  1:2727207/67‑3 (MQ=255)
                               cTGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTtttt                                       <  1:4888679/69‑1 (MQ=255)
                               cTGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTAc                                     <  1:1573822/71‑1 (MQ=255)
                                 gATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTAcgt                                   >  1:5291234/1‑69 (MQ=255)
                                  aTTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTActct                                  >  1:2202953/1‑71 (MQ=255)
                                   ttGCCGAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTActct                                  >  1:3191869/1‑70 (MQ=255)
                                   ttGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTActct                                  >  1:3853554/1‑70 (MQ=255)
                                   ttGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTg                                 <  1:4366441/71‑1 (MQ=255)
                                   ttGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTg                                 >  1:1663345/1‑71 (MQ=255)
                                    tGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTg                                 >  1:992449/1‑70 (MQ=255)
                                    tGCCAAAAAACGTAATCATCCTCATGCCGACGCCAtt                                                                  >  1:2111344/1‑37 (MQ=16)
                                        aaaaaaCGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGt                            >  1:2334898/1‑71 (MQ=255)
                                        aaaaaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGt                            >  1:3214145/1‑71 (MQ=255)
                                        aaaaaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGt                            >  1:660056/1‑71 (MQ=255)
                                         aaaaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGt                            >  1:722329/1‑70 (MQ=255)
                                         aaaaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGt                            >  1:435764/1‑70 (MQ=255)
                                         aaaaGCGCAACCATCCCCAAGCCGACGCTATTCATAcggcgg                                                        >  1:2823040/1‑42 (MQ=255)
                                         aaaaACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGt                            >  1:4942626/1‑70 (MQ=255)
                                           aaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGTCTGGGTGAGCCTGTTTACTCTGCATGTTAt                         >  1:308777/1‑71 (MQ=255)
                                           aaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTgg                                                   >  1:780185/1‑45 (MQ=255)
                                           aaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTgg                                                   >  1:2292439/1‑45 (MQ=255)
                                           aaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTAt                         >  1:1164799/1‑71 (MQ=255)
                                                 aaCCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATc                        <  1:3414335/66‑1 (MQ=255)
                                                  atcATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTa                          >  1:1354295/3‑63 (MQ=255)
                                                   tcATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTa                          <  1:5340909/61‑1 (MQ=255)
                                                   tcATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTa                          <  1:2660539/61‑1 (MQ=255)
                                                   tcATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTa                          <  1:1396186/61‑1 (MQ=255)
                                                   tcATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGt                 <  1:1009023/70‑1 (MQ=255)
                                                   ccATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGt                 <  1:5276981/71‑1 (MQ=255)
                                                   ccATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGt                 <  1:515595/71‑1 (MQ=255)
                                                    cATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGt                            >  1:2114441/1‑59 (MQ=255)
                                                     aTCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGttt               <  1:5020467/71‑1 (MQ=255)
                                                     aTCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGtt                >  1:4722256/1‑70 (MQ=255)
                                                      tCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTAcgt                                   <  1:1721333/50‑3 (MQ=255)
                                                      tCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGtt                                        <  1:2935852/45‑1 (MQ=255)
                                                      tCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTc              <  1:1489890/71‑1 (MQ=255)
                                                        ctcATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTtg            >  1:2797549/3‑71 (MQ=255)
                                                        cccATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCtg            <  1:2518199/71‑1 (MQ=255)
                                                        cccATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCtg            <  1:2724093/71‑1 (MQ=255)
                                                        cccATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCt             <  1:1131859/70‑1 (MQ=255)
                                                        cccATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCt             <  1:3068120/70‑1 (MQ=255)
                                                        cccATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCt             <  1:3899699/70‑1 (MQ=255)
                                                         ccATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGtt                           <  1:3216579/55‑1 (MQ=255)
                                                          cATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCt             <  1:1049063/68‑1 (MQ=255)
                                                          cATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTtgtg          >  1:4692436/1‑71 (MQ=255)
                                                          cATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTtgtg          >  1:2941559/1‑71 (MQ=255)
                                                           aTGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTtg            <  1:5633290/68‑1 (MQ=255)
                                                           aTGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTTGTgg         <  1:1260818/71‑1 (MQ=255)
                                                            tGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGa        <  1:5629547/71‑1 (MQ=255)
                                                            tGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTTGTGGa        <  1:1399860/71‑1 (MQ=255)
                                                             gCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTtg            >  1:1212457/1‑66 (MQ=255)
                                                             gCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTTGTGGAt       >  1:5610432/1‑71 (MQ=255)
                                                              ccGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGAt       >  1:1878292/1‑70 (MQ=255)
                                                                gACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTg    <  1:912649/71‑1 (MQ=255)
                                                                 aCGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGGCGTTTCTGTGGATCTg    <  1:4249926/70‑1 (MQ=255)
                                                                  cGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGt                 <  1:3773534/56‑1 (MQ=255)
                                                                     |                                                                   
TGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGG  >  Exported/2569790‑2569926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: