Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,569,922 C→T 15.4% I64I (ATC→ATT yibI → predicted inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,569,9220CT15.4% 218.8 / 26.1 91I64I (ATC→ATTyibIpredicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (36/41);  new base T (8/6);  total (44/47)
Fisher's exact test for biased strand distribution p-value = 5.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.23e-01

GACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATGTTGCGTCGCAGGAGAAAG  >  Exported/2569854‑2569989
                                                                    |                                                                   
gACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTg                                                                   <  1:912649/71‑1 (MQ=255)
 aCGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGGCGTTTCTGTGGATCTg                                                                   <  1:4249926/70‑1 (MQ=255)
  cGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTTGTGGATTTggg                                                                 <  1:3254356/71‑1 (MQ=255)
         cATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACg                                                            >  1:4651877/1‑69 (MQ=255)
         cATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGc                                                           >  1:876759/1‑70 (MQ=255)
          aTACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGctc                                                         <  1:17661/71‑1 (MQ=255)
            aCGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTg                                                                   >  1:4947383/1‑59 (MQ=255)
               gcggGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGcca                                                              >  1:4891553/1‑59 (MQ=255)
                   gttGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTTGTGGATTTGGGCCACGCTTTa                                                       >  1:5410307/3‑64 (MQ=255)
                   gttGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTTGTGGATTTGGGCCACGCTTTa                                                       >  1:4930081/3‑64 (MQ=255)
                   gCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGa                                                              >  1:1167625/1‑57 (MQ=255)
                   gCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCg                                                <  1:3910979/71‑1 (MQ=255)
                   gCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGAGCGACg                                                            <  1:734327/59‑1 (MQ=255)
                      gggTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAg                                             >  1:2167260/1‑71 (MQ=255)
                      gggTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAg                                             >  1:5049589/1‑71 (MQ=255)
                       ggTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTTGTGGATTTGGGCCACGCTTTACCGCCCGGAg                                             <  1:408269/70‑1 (MQ=255)
                          gAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTTGTGGATTTGGGCCACGCTTTACCGCCCGGAGCggg                                         >  1:2862595/1‑69 (MQ=255)
                          gAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTg                                         >  1:2055722/1‑71 (MQ=255)
                           aGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTgg                                        >  1:1817937/1‑71 (MQ=255)
                           aGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTg                                         <  1:4988941/70‑1 (MQ=255)
                           aGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTg                                         <  1:179309/70‑1 (MQ=255)
                               tGTTTAGTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTgg                                    >  1:738884/1‑71 (MQ=255)
                               tGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTg                                     <  1:264557/70‑1 (MQ=255)
                                gTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATc                                                                     >  1:4858061/1‑37 (MQ=255)
                                gTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATc                                                                     >  1:2290054/1‑37 (MQ=255)
                                gTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGt                                          <  1:3236798/64‑1 (MQ=255)
                                gTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTggg                                   >  1:4756302/1‑71 (MQ=255)
                                 tttACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTgggg                                  <  1:688267/71‑1 (MQ=255)
                                 tttACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTgggg                                  <  1:2954639/71‑1 (MQ=255)
                                 tttACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTgggg                                  <  1:3236960/71‑1 (MQ=255)
                                  ttACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGa                                                              >  1:4199155/1‑42 (MQ=255)
                                  ttACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGa                                                              >  1:1296281/1‑42 (MQ=255)
                                  ttACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGa                                                              >  1:1318107/1‑42 (MQ=255)
                                  ttACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGa                                                              >  1:3539379/1‑42 (MQ=255)
                                  ttACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGa                                                              >  1:3554903/1‑42 (MQ=255)
                                  ttACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGa                                                              >  1:1526812/1‑42 (MQ=255)
                                  ttACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGa                                                              >  1:1533267/1‑42 (MQ=255)
                                  ttACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGc                                            >  1:3863784/1‑60 (MQ=255)
                                  ttACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGc                                            >  1:4911116/1‑60 (MQ=255)
                                  ttACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGc                                            >  1:692925/1‑60 (MQ=255)
                                   tACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAAc                                                  >  1:4468382/1‑53 (MQ=255)
                                   tACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAAc                                                  >  1:976676/1‑53 (MQ=255)
                                   tACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTggg                                   >  1:2295864/1‑68 (MQ=255)
                                   tACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTa                                >  1:4382838/1‑71 (MQ=255)
                                     ctctGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCgg                                               <  1:4119199/54‑1 (MQ=255)
                                       ttacATGTTATCTGGCCGTTTTTGTGGATTTGGGCCACGCTTTACCGCCCGGAGCGGGGATGGGGAATGCa                            <  1:985011/68‑1 (MQ=255)
                                        tacATGTTATCTGGCCGTTTTTGTGGATTTGGGCCACGCTTTACCGCCCGGAGCgggg                                        <  1:600294/56‑1 (MQ=255)
                                        tGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTa                                                       <  1:4915046/43‑1 (MQ=255)
                                         acATGTTATCTGGCCGTTTTTGTGGATTTGGGCCACGCTTTACCGCCCGGAGCGGGGATGGGGAATGCa                            >  1:2574627/2‑69 (MQ=255)
                                         acATGTTATCTGGCCGTTTTTGTGGATTTGGGCCACGCTTTACCGCCCGGAGCGGGGATGGGGAATGCa                            >  1:1599532/2‑69 (MQ=255)
                                          cATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGt                          <  1:2119049/70‑1 (MQ=255)
                                            tGTTATCTGGCCGTTTTTGTGGATTTGGGCCACGCTTTACCGCCCGGAg                                             <  1:34380/49‑1 (MQ=16)
                                            tGTTATCTGGCCGTTTTTGTGGATTTGGGCCACGCTTTACCGCCCGGAg                                             <  1:318272/49‑1 (MQ=16)
                                            tGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAAcc                                                 <  1:4021559/45‑1 (MQ=255)
                                            tGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTcac                       <  1:3253850/71‑1 (MQ=255)
                                            tGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTcac                       <  1:1913031/71‑1 (MQ=255)
                                             gTTATCTGGCCGTTTTTGTGGATTTGGGCCACGCTTTACCGCCCGGAGCGGGGATgg                                    >  1:2496781/1‑57 (MQ=255)
                                             gTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTca                        >  1:4231000/1‑69 (MQ=255)
                                              ttATCTGGCCGTTTTTGTGGATTTGGGCCACGCTTTACCGCCCGGa                                              >  1:999846/1‑46 (MQ=16)
                                              ttATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTa                                >  1:2835761/1‑60 (MQ=255)
                                              ttATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACAt                     <  1:1248647/71‑1 (MQ=255)
                                               tATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTggg                                   >  1:4415900/1‑56 (MQ=255)
                                               tATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATg                    >  1:5597047/1‑71 (MQ=255)
                                                aTCTGGCCGTTTTTGTGGATTTGGGCCACGCTTTACCGCCCGGAGCgggga                                       >  1:2988230/1‑50 (MQ=255)
                                                aTCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGc                             <  1:468464/61‑1 (MQ=255)
                                                    ggCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATg                    <  1:1051868/66‑1 (MQ=255)
                                                    ggCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATg                    <  1:148293/66‑1 (MQ=255)
                                                    ggCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATg                    <  1:638176/66‑1 (MQ=255)
                                                    ggCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATg                    <  1:614750/66‑1 (MQ=255)
                                                    ggCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATg                    <  1:4453950/66‑1 (MQ=255)
                                                    ggCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATGTTGCg               >  1:3336548/1‑71 (MQ=255)
                                                     gCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATGtt                  <  1:4769139/67‑1 (MQ=255)
                                                     gCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATGTTGCGt              <  1:1753047/71‑1 (MQ=255)
                                                       cGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATGTTGCGTCg            <  1:3495332/71‑1 (MQ=255)
                                                       cGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATGTTGCGTCg            <  1:4391573/71‑1 (MQ=255)
                                                        gTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATGTTGCGTCGc           <  1:505330/71‑1 (MQ=255)
                                                         tttCTGTGGATCTGGGCGACGCTCTATCAACCGGa                                              <  1:1133980/35‑1 (MQ=255)
                                                         tttCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTg                                     <  1:718837/44‑1 (MQ=255)
                                                         tttCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTAt                               <  1:2822988/50‑1 (MQ=255)
                                                          ttCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTgggg                                  <  1:3754158/46‑1 (MQ=255)
                                                           tCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGt                                          >  1:5377266/1‑37 (MQ=255)
                                                           tCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTg                                         <  1:1495127/38‑1 (MQ=255)
                                                           tCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGc                                       >  1:5674379/1‑40 (MQ=255)
                                                           tCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTcac                       >  1:3816473/1‑56 (MQ=255)
                                                           tCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATGTTGCGTCGCAg         >  1:578893/1‑70 (MQ=255)
                                                              gtgGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATGTTGCGTCGCAGgag      >  1:5551162/1‑70 (MQ=255)
                                                               tgGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATGTTGCGTCGCAGga       <  1:3482460/68‑1 (MQ=255)
                                                               tgGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATGTTGCGTCGCAGGAGaa    <  1:3762746/71‑1 (MQ=255)
                                                                ggATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGt                                 <  1:5142237/41‑1 (MQ=255)
                                                                ggATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATGTTGCGTCGCAGGAGaa    <  1:3769422/70‑1 (MQ=255)
                                                                 gATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATGTTGCGTCGCAGGAGAAAg  >  1:145935/1‑71 (MQ=255)
                                                                  aTCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTAt                               <  1:55658/41‑1 (MQ=255)
                                                                  aTCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTAt                               <  1:4576953/41‑1 (MQ=255)
                                                                    cTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATGTTGCGt              >  1:4983688/1‑56 (MQ=255)
                                                                    |                                                                   
GACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATGTTGCGTCGCAGGAGAAAG  >  Exported/2569854‑2569989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: