BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	2,570,806	A→G	11.3%	P237P (CCA→CCG)	yibH →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	2,570,806	0	A	G	11.3%	274.9 / 19.6	106	P237P (CCA→CCG)	yibH	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (44/50); new base G (9/3); total (53/53)
Fisher's exact test for biased strand distribution p-value = 1.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

AATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCA  >  Exported/2570736‑2570871

aaTATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCa                                                                   <  1:1698509/71‑1 (MQ=255)

 atatAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAg                                                                  >  1:1906756/1‑71 (MQ=255)

  tataACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAgg                                                                 >  1:3649775/1‑71 (MQ=255)

    taACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTa                                                               >  1:4119278/1‑71 (MQ=255)

    taACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTa                                                               <  1:3749721/71‑1 (MQ=255)

    taACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTa                                                               >  1:1397815/1‑71 (MQ=255)

    taACCTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGATTCGTCCGGGTa                                                               >  1:5628176/1‑71 (MQ=255)

      aCCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACa                                                             <  1:2085988/71‑1 (MQ=255)

        cTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGATTCGTCCGGGTACCTa                                                           >  1:1271195/1‑71 (MQ=255)

          tGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACg                                                         >  1:2174325/1‑71 (MQ=255)

            aGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGc                                                        <  1:1028155/70‑1 (MQ=255)

            aGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGc                                                        <  1:2295133/70‑1 (MQ=255)

               aGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCt                                                    <  1:3225794/71‑1 (MQ=255)

               aGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCt                                                    <  1:3218372/71‑1 (MQ=255)

               aGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCt                                                    <  1:428766/71‑1 (MQ=255)

                gACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACa                                                             <  1:1560887/61‑1 (MQ=255)

                gACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTg                                                   >  1:3098634/1‑71 (MQ=255)

                gACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTg                                                   <  1:2670348/71‑1 (MQ=255)

                  cTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGc                                                     >  1:917187/1‑67 (MQ=255)

                  cTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGc                                                     >  1:3899776/1‑67 (MQ=255)

                  cTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGc                                                     >  1:5506930/1‑67 (MQ=255)

                     tCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCtt                                               <  1:124460/70‑1 (MQ=255)

                       aTTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGcc                                            >  1:2726988/1‑71 (MQ=255)

                        ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                           <  1:3303062/71‑1 (MQ=255)

                        ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                           <  1:3667828/71‑1 (MQ=255)

                        ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                           <  1:3065483/71‑1 (MQ=255)

                        ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                           <  1:4652072/71‑1 (MQ=255)

                        ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                           <  1:5155473/71‑1 (MQ=255)

                        ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                           <  1:1277911/71‑1 (MQ=255)

                        ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                           <  1:96965/71‑1 (MQ=255)

                        ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                           <  1:2016074/71‑1 (MQ=255)

                        ttCGCGCGCCGAGCAAAGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                           <  1:1593534/71‑1 (MQ=255)

                         tCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGc                                          >  1:4405786/1‑71 (MQ=255)

                          cgcgcgCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                           >  1:4819656/1‑69 (MQ=255)

                             gcgcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTAc                                                              >  1:974992/1‑47 (MQ=255)

                             gcgcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTAc                                                              >  1:3886713/1‑47 (MQ=255)

                             gcgcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTAc                                                              >  1:2616414/1‑47 (MQ=255)

                             gcgcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTAc                                                              >  1:3157193/1‑47 (MQ=255)

                             gcgcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTAc                                                              >  1:2029958/1‑47 (MQ=255)

                              cgcCGAGCGATGGTTATGTGACCCAGGTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGt                                     >  1:2187208/1‑71 (MQ=255)

                              cgcCGAGCGATGGTTATGTGACCCAGGTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGt                                     >  1:4854762/1‑71 (MQ=255)

                              cgcCGAGCCATGGTTATGTGACCCAGGTACTGATTCGTCCGGGTACCTatg                                                         >  1:5221741/1‑49 (MQ=255)

                               gcagAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTc                                    >  1:5095792/4‑71 (MQ=255)

                               gcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTc                                    >  1:5027913/1‑71 (MQ=255)

                                  gAGCAATGGCTACGTTACTCAGGTACTGAACCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTcc                                   >  1:2462052/1‑69 (MQ=255)

                                   aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTg                                                   >  1:3905850/1‑52 (MQ=255)

                                   aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTg                                                   >  1:13360/1‑52 (MQ=255)

                                   aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTg                                                   >  1:1658442/1‑52 (MQ=255)

                                    gCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCgg                                 <  1:2791965/69‑1 (MQ=255)

                                       aTGGTTATGTGACCCAGGTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCGGTGATg                            <  1:4950043/71‑1 (MQ=255)

                                       aTGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTc                                    <  1:5461260/63‑1 (MQ=255)

                                        tGGTTATGTGACCCAGGTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCGGTGATgg                           >  1:622311/1‑71 (MQ=255)

                                        tGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGa                              <  1:198856/68‑1 (MQ=255)

                                         ggCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCg                                  >  1:4733665/1‑63 (MQ=255)

                                         ggCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCg                                  >  1:951436/1‑63 (MQ=255)

                                         ggCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATgg                           >  1:2486290/1‑70 (MQ=255)

                                         ggCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGt                          >  1:5129572/1‑71 (MQ=255)

                                         ggCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGt                          >  1:4296935/1‑71 (MQ=255)

                                          gCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTc                         <  1:2328269/71‑1 (MQ=255)

                                          gCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTc                         >  1:3392954/1‑71 (MQ=255)

                                           cTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCt                        <  1:4172809/71‑1 (MQ=255)

                                              cGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCtt                       <  1:1319461/69‑1 (MQ=255)

                                              cGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTc                      <  1:4657486/70‑1 (MQ=255)

                                              cGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCa                     >  1:2203160/1‑71 (MQ=255)

                                               gTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGcc                                            <  1:4329799/47‑1 (MQ=255)

                                                ttACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGc                                          <  1:3557122/48‑1 (MQ=255)

                                                ttACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTc                                    <  1:2937265/54‑1 (MQ=255)

                                                ttACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATc                   >  1:508147/1‑71 (MQ=255)

                                                  aCTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTg                                        >  1:2121406/1‑48 (MQ=255)

                                                  aCTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTg                                        >  1:2601556/1‑48 (MQ=255)

                                                  aCTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATcc                  >  1:415778/1‑70 (MQ=255)

                                                   cTCAGGTACTGATCCGCCCAGGTACATACGCAGCTg                                                   <  1:3699059/36‑1 (MQ=255)

                                                   cTCAGGTACTGATCCGCCCAGGTACATACGCAGCTg                                                   <  1:879172/36‑1 (MQ=255)

                                                      aGGTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCg                                  >  1:126606/1‑50 (MQ=255)

                                                      aGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGc                                          >  1:4093893/1‑42 (MQ=255)

                                                      aGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTc                         >  1:4223055/1‑59 (MQ=255)

                                                       ggTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAg             >  1:976414/1‑70 (MQ=255)

                                                       ggTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAg             >  1:3890918/1‑70 (MQ=255)

                                                        gTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCGGTGATGGTGTTTATACCCGATCa           <  1:2235105/71‑1 (MQ=255)

                                                        gTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTg                               <  1:632709/51‑1 (MQ=255)

                                                        gTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCtt                       <  1:4955373/59‑1 (MQ=255)

                                                        gTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCtt                       <  1:2622058/59‑1 (MQ=255)

                                                        gTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCtt                       <  1:261161/59‑1 (MQ=255)

                                                        gTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCtt                       <  1:554302/59‑1 (MQ=255)

                                                          aCTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCGGTGATGGTGTTTATACCCGATCag          >  1:5457882/1‑69 (MQ=255)

                                                           cTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCGGTGATGGTGTTTATACCCGATCaga         <  1:1211790/70‑3 (MQ=255)

                                                           cTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaaa        <  1:3691402/71‑1 (MQ=255)

                                                           cTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaa         <  1:3172040/70‑1 (MQ=255)

                                                           cTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaa         <  1:1549848/70‑1 (MQ=255)

                                                           cTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaa         <  1:2417283/70‑1 (MQ=255)

                                                             gATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATccc                 >  1:1758223/1‑60 (MQ=255)

                                                             gATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCa           >  1:1108039/1‑66 (MQ=255)

                                                              aTCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaaaa       <  1:5462102/69‑1 (MQ=255)

                                                              aTCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAAc      <  1:4349864/70‑1 (MQ=255)

                                                               tCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCAt                    >  1:2717923/1‑55 (MQ=255)

                                                               tCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACgg    >  1:4744885/1‑71 (MQ=255)

                                                                 cGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCa  <  1:2159368/71‑1 (MQ=255)

                                                                 cGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCa  <  1:2631997/71‑1 (MQ=255)

                                                                   cccAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCa  <  1:1483664/69‑1 (MQ=255)

                                                                     cAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCt                        <  1:1420553/45‑1 (MQ=255)

AATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCA  >  Exported/2570736‑2570871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`