BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	2,570,812	A→C	10.8%	T239T (ACA→ACC)	yibH →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	2,570,812	0	A	C	10.8%	235.4 / 22.4	101	T239T (ACA→ACC)	yibH	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (40/50); new base C (8/3); total (48/53)
Fisher's exact test for biased strand distribution p-value = 1.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

Only 100 of 103 total aligned reads displayed.

ACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGC  >  Exported/2570742‑2570880

aCCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACa                                                                      <  1:2085988/71‑1 (MQ=255)

  cTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGATTCGTCCGGGTACCTa                                                                    >  1:1271195/1‑71 (MQ=255)

    tGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACg                                                                  >  1:2174325/1‑71 (MQ=255)

      aGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGc                                                                 <  1:1028155/70‑1 (MQ=255)

      aGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGc                                                                 <  1:2295133/70‑1 (MQ=255)

         aGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCt                                                             <  1:428766/71‑1 (MQ=255)

         aGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCt                                                             <  1:3225794/71‑1 (MQ=255)

         aGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCt                                                             <  1:3218372/71‑1 (MQ=255)

          gACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACa                                                                      <  1:1560887/61‑1 (MQ=255)

          gACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTg                                                            >  1:3098634/1‑71 (MQ=255)

          gACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTg                                                            <  1:2670348/71‑1 (MQ=255)

            cTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGc                                                              >  1:5506930/1‑67 (MQ=255)

            cTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGc                                                              >  1:3899776/1‑67 (MQ=255)

            cTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGc                                                              >  1:917187/1‑67 (MQ=255)

               tCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCtt                                                        <  1:124460/70‑1 (MQ=255)

                 aTTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGcc                                                     >  1:2726988/1‑71 (MQ=255)

                  ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                    <  1:3065483/71‑1 (MQ=255)

                  ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                    <  1:3303062/71‑1 (MQ=255)

                  ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                    <  1:3667828/71‑1 (MQ=255)

                  ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                    <  1:4652072/71‑1 (MQ=255)

                  ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                    <  1:96965/71‑1 (MQ=255)

                  ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                    <  1:2016074/71‑1 (MQ=255)

                  ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                    <  1:1277911/71‑1 (MQ=255)

                  ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                    <  1:5155473/71‑1 (MQ=255)

                  ttCGCGCGCCGAGCAAAGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                    <  1:1593534/71‑1 (MQ=255)

                   tCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGc                                                   >  1:4405786/1‑71 (MQ=255)

                    cgcgcgCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                    >  1:4819656/1‑69 (MQ=255)

                        cgcCGAGCGATGGTTATGTGACCCAGGTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGt                                              >  1:2187208/1‑71 (MQ=255)

                        cgcCGAGCGATGGTTATGTGACCCAGGTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGt                                              >  1:4854762/1‑71 (MQ=255)

                        cgcCGAGCCATGGTTATGTGACCCAGGTACTGATTCGTCCGGGTACCTatg                                                                  >  1:5221741/1‑49 (MQ=255)

                         gcagAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTc                                             >  1:5095792/4‑71 (MQ=255)

                         gcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTc                                             >  1:5027913/1‑71 (MQ=255)

                            gAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCgg                                          <  1:4523482/71‑1 (MQ=255)

                             aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTg                                                            >  1:13360/1‑52 (MQ=255)

                             aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTg                                                            >  1:1658442/1‑52 (MQ=255)

                             aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTg                                                            >  1:3905850/1‑52 (MQ=255)

                              gCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCgg                                          <  1:3771478/69‑1 (MQ=255)

                              gCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCgg                                          <  1:2791965/69‑1 (MQ=255)

                                 aTGGTTATGTGACCCAGGTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCGGTGATg                                     >  1:544441/1‑71 (MQ=255)

                                 aTGGTTATGTGACCCAGGTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCGGTGATg                                     <  1:4950043/71‑1 (MQ=255)

                                 aTGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTc                                             <  1:5461260/63‑1 (MQ=255)

                                 aTGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTc                                             <  1:2249279/63‑1 (MQ=255)

                                  tGGTTATGTGACCCAGGTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCGGTGATgg                                    >  1:622311/1‑71 (MQ=255)

                                  tGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGa                                       <  1:198856/68‑1 (MQ=255)

                                   ggCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCg                                           >  1:4733665/1‑63 (MQ=255)

                                   ggCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCg                                           >  1:951436/1‑63 (MQ=255)

                                   ggCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATgg                                    >  1:2486290/1‑70 (MQ=255)

                                   ggCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGt                                   >  1:5129572/1‑71 (MQ=255)

                                    gCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTc                                  >  1:3392954/1‑71 (MQ=255)

                                    gCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTc                                  <  1:2328269/71‑1 (MQ=255)

                                     cTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCt                                 <  1:4172809/71‑1 (MQ=255)

                                        cGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCtt                                <  1:1319461/69‑1 (MQ=255)

                                        cGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTc                               <  1:4657486/70‑1 (MQ=255)

                                        cGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCa                              >  1:2203160/1‑71 (MQ=255)

                                         gTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGcc                                                     <  1:4329799/47‑1 (MQ=255)

                                          ttACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGc                                                   <  1:3557122/48‑1 (MQ=255)

                                          ttACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTc                                             <  1:2937265/54‑1 (MQ=255)

                                          ttACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATc                            >  1:508147/1‑71 (MQ=255)

                                            aCTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTg                                                 >  1:2121406/1‑48 (MQ=255)

                                            aCTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTg                                                 >  1:2601556/1‑48 (MQ=255)

                                            aCTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATcc                           >  1:415778/1‑70 (MQ=255)

                                             cTCAGGTACTGATCCGCCCAGGTACATACGCAGCTg                                                            <  1:3699059/36‑1 (MQ=255)

                                             cTCAGGTACTGATCCGCCCAGGTACATACGCAGCTg                                                            <  1:879172/36‑1 (MQ=255)

                                                aGGTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCg                                           >  1:126606/1‑50 (MQ=255)

                                                aGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGc                                                   >  1:4093893/1‑42 (MQ=255)

                                                aGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTc                                  >  1:4223055/1‑59 (MQ=255)

                                                aGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAg                      >  1:2878984/1‑71 (MQ=255)

                                                 ggTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAg                      >  1:976414/1‑70 (MQ=255)

                                                 ggTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAg                      >  1:3890918/1‑70 (MQ=255)

                                                  gTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCGGTGATGGTGTTTATACCCGATCa                    <  1:2235105/71‑1 (MQ=255)

                                                  gTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTg                                        <  1:632709/51‑1 (MQ=255)

                                                  gTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCtt                                <  1:554302/59‑1 (MQ=255)

                                                  gTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCtt                                <  1:2622058/59‑1 (MQ=255)

                                                  gTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCtt                                <  1:4955373/59‑1 (MQ=255)

                                                  gTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCtt                                <  1:261161/59‑1 (MQ=255)

                                                    aCTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCGGTGATGGTGTTTATACCCGATCag                   >  1:5457882/1‑69 (MQ=255)

                                                     cTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCGGTGATGGTGTTTATACCCGATCaga                  <  1:1211790/70‑3 (MQ=255)

                                                     cTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaaa                 <  1:3691402/71‑1 (MQ=255)

                                                     cTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaa                  <  1:2417283/70‑1 (MQ=255)

                                                     cTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaa                  <  1:1549848/70‑1 (MQ=255)

                                                     cTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaa                  <  1:1625402/70‑1 (MQ=255)

                                                     cTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaa                  <  1:3172040/70‑1 (MQ=255)

                                                       gATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATccc                          >  1:1758223/1‑60 (MQ=255)

                                                       gATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCa                    >  1:1108039/1‑66 (MQ=255)

                                                        aTCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaaaa                <  1:5462102/69‑1 (MQ=255)

                                                        aTCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAAc               <  1:4349864/70‑1 (MQ=255)

                                                         tCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCAt                             >  1:2717923/1‑55 (MQ=255)

                                                         tCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACgg             >  1:4744885/1‑71 (MQ=255)

                                                           cGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCa           <  1:2159368/71‑1 (MQ=255)

                                                           cGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCa           <  1:2631997/71‑1 (MQ=255)

                                                             cccAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCa           <  1:1483664/69‑1 (MQ=255)

                                                               cAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCt                                 <  1:1420553/45‑1 (MQ=255)

                                                                 ggTACATACGCAGCTGCCTTGCCGCTGCGTACGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGt     >  1:1046851/1‑71 (MQ=255)

                                                                  gTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaaa                 <  1:2733484/58‑1 (MQ=255)

                                                                  gTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTc    <  1:1125899/71‑1 (MQ=255)

                                                                    aCATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTc                               >  1:1756262/1‑42 (MQ=255)

                                                                    aCATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCa                              >  1:1705886/1‑43 (MQ=255)

                                                                    aCATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGc  >  1:4283257/1‑71 (MQ=255)

                                                                     cATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTc                               >  1:802483/1‑41 (MQ=255)

                                                                     cATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTc                               <  1:600178/41‑1 (MQ=255)

ACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGC  >  Exported/2570742‑2570880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`