BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	2,570,815	C→T	8.5%	Y240Y (TAC→TAT)	yibH →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	2,570,815	0	C	T	8.5%	300.6 / 12.4	106	Y240Y (TAC→TAT)	yibH	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (42/55); new base T (6/3); total (48/58)
Fisher's exact test for biased strand distribution p-value = 2.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.96e-01

Only 100 of 109 total aligned reads displayed.

TGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAAT  >  Exported/2570746‑2570885

tGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACg                                                                       >  1:2174325/1‑71 (MQ=255)

  aGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGc                                                                      <  1:1028155/70‑1 (MQ=255)

  aGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGc                                                                      <  1:2295133/70‑1 (MQ=255)

     aGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCt                                                                  <  1:428766/71‑1 (MQ=255)

     aGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCt                                                                  <  1:3218372/71‑1 (MQ=255)

     aGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCt                                                                  <  1:3225794/71‑1 (MQ=255)

      gACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTg                                                                 <  1:2670348/71‑1 (MQ=255)

      gACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTg                                                                 >  1:3098634/1‑71 (MQ=255)

        cTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGc                                                                   >  1:3899776/1‑67 (MQ=255)

        cTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGc                                                                   >  1:917187/1‑67 (MQ=255)

           tCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCtt                                                             <  1:124460/70‑1 (MQ=255)

             aTTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGcc                                                          >  1:2726988/1‑71 (MQ=255)

              ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                         <  1:5155473/71‑1 (MQ=255)

              ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                         <  1:4652072/71‑1 (MQ=255)

              ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                         <  1:1277911/71‑1 (MQ=255)

              ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                         <  1:3667828/71‑1 (MQ=255)

              ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                         <  1:2016074/71‑1 (MQ=255)

              ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                         <  1:3303062/71‑1 (MQ=255)

              ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                         <  1:96965/71‑1 (MQ=255)

              ttCGCGCGCCGAGCAAAGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                         <  1:1593534/71‑1 (MQ=255)

               tCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGc                                                        >  1:4405786/1‑71 (MQ=255)

                cgcgcgCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                         >  1:4819656/1‑69 (MQ=255)

                    cgcCGAGCGATGGTTATGTGACCCAGGTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGt                                                   >  1:4854762/1‑71 (MQ=255)

                    cgcCGAGCGATGGTTATGTGACCCAGGTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGt                                                   >  1:2187208/1‑71 (MQ=255)

                     gcagAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTc                                                  >  1:5095792/4‑71 (MQ=255)

                     gcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTc                                                  >  1:5027913/1‑71 (MQ=255)

                        gAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCgg                                               <  1:4523482/71‑1 (MQ=255)

                        gAGCAATGGCTACGTTACTCAGGTACTGAACCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTcc                                                 >  1:2462052/1‑69 (MQ=255)

                         aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTg                                                                 >  1:3905850/1‑52 (MQ=255)

                         aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTg                                                                 >  1:1658442/1‑52 (MQ=255)

                         aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTg                                                                 >  1:13360/1‑52 (MQ=255)

                          gCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCgg                                               <  1:2791965/69‑1 (MQ=255)

                             aTGGTTATGTGACCCAGGTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCGGTGATg                                          >  1:544441/1‑71 (MQ=255)

                             aTGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTc                                                  <  1:5461260/63‑1 (MQ=255)

                             aTGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTc                                                  <  1:2249279/63‑1 (MQ=255)

                              tGGTTATGTGACCCAGGTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCGGTGATgg                                         >  1:622311/1‑71 (MQ=255)

                              tGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGa                                            <  1:198856/68‑1 (MQ=255)

                               ggCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCg                                                >  1:951436/1‑63 (MQ=255)

                               ggCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCg                                                >  1:4733665/1‑63 (MQ=255)

                               ggCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGt                                        >  1:5129572/1‑71 (MQ=255)

                               ggCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGt                                        >  1:4296935/1‑71 (MQ=255)

                                gCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTc                                       <  1:2328269/71‑1 (MQ=255)

                                gCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTc                                       >  1:3392954/1‑71 (MQ=255)

                                 cTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCt                                      <  1:4172809/71‑1 (MQ=255)

                                    cGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCtt                                     <  1:1319461/69‑1 (MQ=255)

                                    cGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTc                                    <  1:4657486/70‑1 (MQ=255)

                                    cGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCa                                   >  1:2203160/1‑71 (MQ=255)

                                      ttACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGc                                                        <  1:3557122/48‑1 (MQ=255)

                                      ttACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTc                                                  <  1:2937265/54‑1 (MQ=255)

                                      ttACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATc                                 >  1:508147/1‑71 (MQ=255)

                                        aCTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTg                                                      >  1:2601556/1‑48 (MQ=255)

                                        aCTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTg                                                      >  1:2121406/1‑48 (MQ=255)

                                        aCTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATcc                                >  1:415778/1‑70 (MQ=255)

                                         cTCAGGTACTGATCCGCCCAGGTACATACGCAGCTg                                                                 <  1:879172/36‑1 (MQ=255)

                                         cTCAGGTACTGATCCGCCCAGGTACATACGCAGCTg                                                                 <  1:3699059/36‑1 (MQ=255)

                                            aGGTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCg                                                >  1:126606/1‑50 (MQ=255)

                                            aGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGc                                                        >  1:4093893/1‑42 (MQ=255)

                                            aGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTc                                       >  1:4223055/1‑59 (MQ=255)

                                            aGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAg                           >  1:2878984/1‑71 (MQ=255)

                                             ggTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAg                           >  1:3890918/1‑70 (MQ=255)

                                             ggTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAg                           >  1:976414/1‑70 (MQ=255)

                                              gTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCGGTGATGGTGTTTATACCCGATCa                         <  1:2235105/71‑1 (MQ=255)

                                              gTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTg                                             <  1:632709/51‑1 (MQ=255)

                                              gTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCtt                                     <  1:554302/59‑1 (MQ=255)

                                              gTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCtt                                     <  1:261161/59‑1 (MQ=255)

                                              gTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCtt                                     <  1:4955373/59‑1 (MQ=255)

                                              gTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCtt                                     <  1:2622058/59‑1 (MQ=255)

                                                aCTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCGGTGATGGTGTTTATACCCGATCag                        >  1:5457882/1‑69 (MQ=255)

                                                 cTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCGGTGATGGTGTTTATACCCGATCaga                       <  1:1211790/70‑3 (MQ=255)

                                                 cTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaaa                      <  1:3691402/71‑1 (MQ=255)

                                                 cTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaa                       <  1:3172040/70‑1 (MQ=255)

                                                 cTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaa                       <  1:2417283/70‑1 (MQ=255)

                                                 cTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaa                       <  1:1549848/70‑1 (MQ=255)

                                                 cTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaa                       <  1:1625402/70‑1 (MQ=255)

                                                   gATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATccc                               >  1:1758223/1‑60 (MQ=255)

                                                   gATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCa                         >  1:1108039/1‑66 (MQ=255)

                                                    aTCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaaaa                     <  1:5462102/69‑1 (MQ=255)

                                                    aTCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAAc                    <  1:4349864/70‑1 (MQ=255)

                                                     tCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCAt                                  >  1:2717923/1‑55 (MQ=255)

                                                     tCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACgg                  >  1:4744885/1‑71 (MQ=255)

                                                       cGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCa                <  1:2631997/71‑1 (MQ=255)

                                                       cGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCa                <  1:2159368/71‑1 (MQ=255)

                                                           cAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCt                                      <  1:1420553/45‑1 (MQ=255)

                                                             ggTACATACGCAGCTGCCTTGCCGCTGCGTACGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGt          >  1:1046851/1‑71 (MQ=255)

                                                              gTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaaa                      <  1:2733484/58‑1 (MQ=255)

                                                              gTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTc         <  1:1125899/71‑1 (MQ=255)

                                                                aCATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTc                                    >  1:1756262/1‑42 (MQ=255)

                                                                aCATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCa                                   >  1:1705886/1‑43 (MQ=255)

                                                                aCATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGc       >  1:4283257/1‑71 (MQ=255)

                                                                 cATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTc                                    <  1:600178/41‑1 (MQ=255)

                                                                 cATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTc                                    >  1:802483/1‑41 (MQ=255)

                                                                   tACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTc                                    <  1:396009/39‑1 (MQ=255)

                                                                   tACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTc                                    <  1:1082178/39‑1 (MQ=255)

                                                                   tACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGc                          <  1:2466572/49‑1 (MQ=255)

                                                                   tACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaa                       <  1:5627644/52‑1 (MQ=255)

                                                                   tACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaa                       <  1:1697807/52‑1 (MQ=255)

                                                                    aCGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCaa   >  1:5118750/1‑71 (MQ=255)

                                                                    aCGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCaa   >  1:3306124/1‑71 (MQ=255)

                                                                     cGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAAc                    <  1:4641982/53‑1 (MQ=255)

                                                                     cGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAAt  >  1:4691553/1‑71 (MQ=255)

TGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAAT  >  Exported/2570746‑2570885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`