BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	2,570,818	A→C	8.2%	A241A (GCA→GCC)	yibH →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	2,570,818	0	A	C	8.2%	279.4 / 17.3	110	A241A (GCA→GCC)	yibH	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (44/57); new base C (6/3); total (50/60)
Fisher's exact test for biased strand distribution p-value = 2.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 112 total aligned reads displayed.

GCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTC  >  Exported/2570749‑2570888

  aGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCt                                                                     <  1:3225794/71‑1 (MQ=255)

  aGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCt                                                                     <  1:3218372/71‑1 (MQ=255)

  aGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCt                                                                     <  1:428766/71‑1 (MQ=255)

   gACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTg                                                                    >  1:3098634/1‑71 (MQ=255)

   gACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTg                                                                    <  1:2670348/71‑1 (MQ=255)

     cTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGc                                                                      >  1:3899776/1‑67 (MQ=255)

     cTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGc                                                                      >  1:917187/1‑67 (MQ=255)

     cTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGc                                                                      >  1:5506930/1‑67 (MQ=255)

        tCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCtt                                                                <  1:124460/70‑1 (MQ=255)

          aTTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGcc                                                             >  1:2726988/1‑71 (MQ=255)

           ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                            <  1:96965/71‑1 (MQ=255)

           ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                            <  1:3667828/71‑1 (MQ=255)

           ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                            <  1:3303062/71‑1 (MQ=255)

           ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                            <  1:3065483/71‑1 (MQ=255)

           ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                            <  1:4652072/71‑1 (MQ=255)

           ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                            <  1:2016074/71‑1 (MQ=255)

           ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                            <  1:1277911/71‑1 (MQ=255)

           ttCGCGCGCCGAGCAAAGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                            <  1:1593534/71‑1 (MQ=255)

            tCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGc                                                           >  1:4405786/1‑71 (MQ=255)

             cgcgcgCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                            >  1:4819656/1‑69 (MQ=255)

                 cgcCGAGCGATGGTTATGTGACCCAGGTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGt                                                      >  1:2187208/1‑71 (MQ=255)

                 cgcCGAGCGATGGTTATGTGACCCAGGTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGt                                                      >  1:4854762/1‑71 (MQ=255)

                  gcagAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTc                                                     >  1:5095792/4‑71 (MQ=255)

                  gcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTc                                                     >  1:5027913/1‑71 (MQ=255)

                     gAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCgg                                                  <  1:4523482/71‑1 (MQ=255)

                     gAGCAATGGCTACGTTACTCAGGTACTGAACCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTcc                                                    >  1:2462052/1‑69 (MQ=255)

                      aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTg                                                                    >  1:3905850/1‑52 (MQ=255)

                      aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTg                                                                    >  1:1658442/1‑52 (MQ=255)

                       gCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCgg                                                  <  1:2791965/69‑1 (MQ=255)

                       gCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCgg                                                  <  1:3771478/69‑1 (MQ=255)

                          aTGGTTATGTGACCCAGGTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCGGTGATg                                             <  1:4950043/71‑1 (MQ=255)

                          aTGGTTATGTGACCCAGGTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCGGTGATg                                             >  1:544441/1‑71 (MQ=255)

                          aTGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTc                                                     <  1:5461260/63‑1 (MQ=255)

                          aTGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTc                                                     <  1:2249279/63‑1 (MQ=255)

                           tGGTTATGTGACCCAGGTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCGGTGATgg                                            >  1:622311/1‑71 (MQ=255)

                           tGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGa                                               <  1:198856/68‑1 (MQ=255)

                            ggCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCg                                                   >  1:951436/1‑63 (MQ=255)

                            ggCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCg                                                   >  1:4733665/1‑63 (MQ=255)

                            ggCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATgg                                            >  1:2486290/1‑70 (MQ=255)

                            ggCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGt                                           >  1:5129572/1‑71 (MQ=255)

                            ggCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGt                                           >  1:4296935/1‑71 (MQ=255)

                             gCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTc                                          <  1:2328269/71‑1 (MQ=255)

                              cTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCt                                         <  1:4172809/71‑1 (MQ=255)

                                 cGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCtt                                        <  1:1319461/69‑1 (MQ=255)

                                 cGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTc                                       <  1:4657486/70‑1 (MQ=255)

                                  gTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGcc                                                             <  1:4329799/47‑1 (MQ=255)

                                   ttACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGc                                                           <  1:3557122/48‑1 (MQ=255)

                                   ttACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTc                                                     <  1:2937265/54‑1 (MQ=255)

                                   ttACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATc                                    >  1:508147/1‑71 (MQ=255)

                                     aCTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTg                                                         >  1:2601556/1‑48 (MQ=255)

                                     aCTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTg                                                         >  1:2121406/1‑48 (MQ=255)

                                     aCTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATcc                                   >  1:415778/1‑70 (MQ=255)

                                      cTCAGGTACTGATCCGCCCAGGTACATACGCAGCTg                                                                    <  1:879172/36‑1 (MQ=255)

                                      cTCAGGTACTGATCCGCCCAGGTACATACGCAGCTg                                                                    <  1:3699059/36‑1 (MQ=255)

                                         aGGTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCg                                                   >  1:126606/1‑50 (MQ=255)

                                         aGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTc                                          >  1:4223055/1‑59 (MQ=255)

                                          ggTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAg                              >  1:976414/1‑70 (MQ=255)

                                          ggTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAg                              >  1:3890918/1‑70 (MQ=255)

                                           gTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCGGTGATGGTGTTTATACCCGATCa                            <  1:2235105/71‑1 (MQ=255)

                                           gTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTg                                                <  1:632709/51‑1 (MQ=255)

                                           gTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCtt                                        <  1:4955373/59‑1 (MQ=255)

                                           gTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCtt                                        <  1:2622058/59‑1 (MQ=255)

                                           gTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCtt                                        <  1:554302/59‑1 (MQ=255)

                                             aCTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCGGTGATGGTGTTTATACCCGATCag                           >  1:5457882/1‑69 (MQ=255)

                                              cTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCGGTGATGGTGTTTATACCCGATCaga                          <  1:1211790/70‑3 (MQ=255)

                                              cTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaaa                         <  1:3691402/71‑1 (MQ=255)

                                              cTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaa                          <  1:3172040/70‑1 (MQ=255)

                                              cTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaa                          <  1:1549848/70‑1 (MQ=255)

                                              cTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaa                          <  1:1625402/70‑1 (MQ=255)

                                              cTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaa                          <  1:2417283/70‑1 (MQ=255)

                                                gATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATccc                                  >  1:1758223/1‑60 (MQ=255)

                                                gATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCa                            >  1:1108039/1‑66 (MQ=255)

                                                 aTCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaaaa                        <  1:5462102/69‑1 (MQ=255)

                                                  tCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCAt                                     >  1:2717923/1‑55 (MQ=255)

                                                    cGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCa                   <  1:2631997/71‑1 (MQ=255)

                                                    cGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCa                   <  1:2159368/71‑1 (MQ=255)

                                                      cccAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCa                   <  1:1483664/69‑1 (MQ=255)

                                                        cAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCt                                         <  1:1420553/45‑1 (MQ=255)

                                                          ggTACATACGCAGCTGCCTTGCCGCTGCGTACGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGt             >  1:1046851/1‑71 (MQ=255)

                                                           gTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaaa                         <  1:2733484/58‑1 (MQ=255)

                                                           gTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTc            <  1:1125899/71‑1 (MQ=255)

                                                             aCATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTc                                       >  1:1756262/1‑42 (MQ=255)

                                                             aCATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGc          >  1:4283257/1‑71 (MQ=255)

                                                              cATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTc                                       >  1:802483/1‑41 (MQ=255)

                                                                tACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTc                                       <  1:396009/39‑1 (MQ=255)

                                                                tACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTc                                       <  1:1082178/39‑1 (MQ=255)

                                                                tACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGc                             <  1:2466572/49‑1 (MQ=255)

                                                                tACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaa                          <  1:5627644/52‑1 (MQ=255)

                                                                tACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaa                          <  1:1697807/52‑1 (MQ=255)

                                                                 aCGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCaa      >  1:5118750/1‑71 (MQ=255)

                                                                 aCGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCaa      >  1:3306124/1‑71 (MQ=255)

                                                                  cGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAAc                       <  1:4641982/53‑1 (MQ=255)

                                                                  cGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAAt     >  1:2123252/1‑71 (MQ=255)

                                                                  cGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAAt     >  1:4691553/1‑71 (MQ=255)

                                                                   gCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaa                          <  1:4283010/49‑1 (MQ=255)

                                                                    cAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAAttt   <  1:2737867/71‑1 (MQ=255)

                                                                    cAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAAttt   <  1:2527354/71‑1 (MQ=255)

                                                                    cAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAAttt   >  1:1563921/1‑71 (MQ=255)

                                                                     aGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTc  <  1:447287/71‑1 (MQ=255)

                                                                     aGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTc  <  1:5590133/71‑1 (MQ=255)

GCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTC  >  Exported/2570749‑2570888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`