BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	2,570,822	G→T	8.8%	A243S (GCC→TCC) ‡	yibH →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	2,570,822	0	G	T	8.8%	320.5 / 8.5	101	A243S (GCC→TCC) ‡	yibH	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (38/54); new base T (6/3); total (44/57)
Fisher's exact test for biased strand distribution p-value = 1.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 108 total aligned reads displayed.

GACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGG  >  Exported/2570752‑2570890

gACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTg                                                                      >  1:3098634/1‑71 (MQ=255)

     tCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCtt                                                                  <  1:124460/70‑1 (MQ=255)

       aTTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGcc                                                               >  1:2726988/1‑71 (MQ=255)

        ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                              <  1:96965/71‑1 (MQ=255)

        ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                              <  1:3065483/71‑1 (MQ=255)

        ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                              <  1:3303062/71‑1 (MQ=255)

        ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                              <  1:3667828/71‑1 (MQ=255)

        ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                              <  1:2016074/71‑1 (MQ=255)

        ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                              <  1:4652072/71‑1 (MQ=255)

        ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                              <  1:5155473/71‑1 (MQ=255)

        ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                              <  1:1277911/71‑1 (MQ=255)

        ttCGCGCGCCGAGCAAAGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                              <  1:1593534/71‑1 (MQ=255)

         tCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGc                                                             >  1:4405786/1‑71 (MQ=255)

          cgcgcgCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                              >  1:4819656/1‑69 (MQ=255)

              cgcCGAGCGATGGTTATGTGACCCAGGTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGt                                                        >  1:4854762/1‑71 (MQ=255)

               gcagAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTc                                                       >  1:5095792/4‑71 (MQ=255)

               gcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTc                                                       >  1:5027913/1‑71 (MQ=255)

                  gAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCgg                                                    <  1:4523482/71‑1 (MQ=255)

                  gAGCAATGGCTACGTTACTCAGGTACTGAACCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTcc                                                      >  1:2462052/1‑69 (MQ=255)

                   aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTg                                                                      >  1:13360/1‑52 (MQ=255)

                   aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTg                                                                      >  1:3905850/1‑52 (MQ=255)

                   aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTg                                                                      >  1:1658442/1‑52 (MQ=255)

                    gCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCgg                                                    <  1:2791965/69‑1 (MQ=255)

                    gCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCgg                                                    <  1:3771478/69‑1 (MQ=255)

                       aTGGTTATGTGACCCAGGTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCGGTGATg                                               <  1:4950043/71‑1 (MQ=255)

                       aTGGTTATGTGACCCAGGTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCGGTGATg                                               >  1:544441/1‑71 (MQ=255)

                       aTGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTc                                                       <  1:2249279/63‑1 (MQ=255)

                       aTGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTc                                                       <  1:5461260/63‑1 (MQ=255)

                        tGGTTATGTGACCCAGGTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCGGTGATgg                                              >  1:622311/1‑71 (MQ=255)

                        tGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGa                                                 <  1:198856/68‑1 (MQ=255)

                         ggCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCg                                                     >  1:951436/1‑63 (MQ=255)

                         ggCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATgg                                              >  1:2486290/1‑70 (MQ=255)

                         ggCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGt                                             >  1:5129572/1‑71 (MQ=255)

                         ggCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGt                                             >  1:4296935/1‑71 (MQ=255)

                          gCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTc                                            >  1:3392954/1‑71 (MQ=255)

                          gCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTc                                            <  1:2328269/71‑1 (MQ=255)

                           cTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCt                                           <  1:4172809/71‑1 (MQ=255)

                              cGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCtt                                          <  1:1319461/69‑1 (MQ=255)

                              cGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTc                                         <  1:4657486/70‑1 (MQ=255)

                              cGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCa                                        >  1:2203160/1‑71 (MQ=255)

                               gTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGcc                                                               <  1:4329799/47‑1 (MQ=255)

                                ttACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGc                                                             <  1:3557122/48‑1 (MQ=255)

                                ttACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTc                                                       <  1:2937265/54‑1 (MQ=255)

                                ttACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATc                                      >  1:508147/1‑71 (MQ=255)

                                  aCTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTg                                                           >  1:2121406/1‑48 (MQ=255)

                                  aCTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTg                                                           >  1:2601556/1‑48 (MQ=255)

                                  aCTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATcc                                     >  1:415778/1‑70 (MQ=255)

                                   cTCAGGTACTGATCCGCCCAGGTACATACGCAGCTg                                                                      <  1:879172/36‑1 (MQ=255)

                                   cTCAGGTACTGATCCGCCCAGGTACATACGCAGCTg                                                                      <  1:3699059/36‑1 (MQ=255)

                                      aGGTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCg                                                     >  1:126606/1‑50 (MQ=255)

                                      aGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGc                                                             >  1:4093893/1‑42 (MQ=255)

                                      aGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAg                                >  1:2878984/1‑71 (MQ=255)

                                       ggTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAg                                >  1:3890918/1‑70 (MQ=255)

                                        gTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCGGTGATGGTGTTTATACCCGATCa                              <  1:2235105/71‑1 (MQ=255)

                                        gTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTg                                                  <  1:632709/51‑1 (MQ=255)

                                        gTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCtt                                          <  1:261161/59‑1 (MQ=255)

                                        gTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCtt                                          <  1:2622058/59‑1 (MQ=255)

                                        gTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCtt                                          <  1:554302/59‑1 (MQ=255)

                                        gTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCtt                                          <  1:4955373/59‑1 (MQ=255)

                                           cTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCGGTGATGGTGTTTATACCCGATCaga                            <  1:1211790/70‑3 (MQ=255)

                                           cTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaaa                           <  1:3691402/71‑1 (MQ=255)

                                           cTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaa                            <  1:1625402/70‑1 (MQ=255)

                                           cTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaa                            <  1:2417283/70‑1 (MQ=255)

                                           cTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaa                            <  1:1549848/70‑1 (MQ=255)

                                           cTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaa                            <  1:3172040/70‑1 (MQ=255)

                                             gATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATccc                                    >  1:1758223/1‑60 (MQ=255)

                                             gATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCa                              >  1:1108039/1‑66 (MQ=255)

                                              aTCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaaaa                          <  1:5462102/69‑1 (MQ=255)

                                              aTCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAAc                         <  1:4349864/70‑1 (MQ=255)

                                               tCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCAt                                       >  1:2717923/1‑55 (MQ=255)

                                               tCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACgg                       >  1:4744885/1‑71 (MQ=255)

                                                 cGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCa                     <  1:2631997/71‑1 (MQ=255)

                                                 cGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCa                     <  1:2159368/71‑1 (MQ=255)

                                                   cccAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCa                     <  1:1483664/69‑1 (MQ=255)

                                                     cAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCt                                           <  1:1420553/45‑1 (MQ=255)

                                                       ggTACATACGCAGCTGCCTTGCCGCTGCGTACGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGt               >  1:1046851/1‑71 (MQ=255)

                                                        gTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaaa                           <  1:2733484/58‑1 (MQ=255)

                                                        gTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTc              <  1:1125899/71‑1 (MQ=255)

                                                          aCATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTc                                         >  1:1756262/1‑42 (MQ=255)

                                                          aCATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCa                                        >  1:1705886/1‑43 (MQ=255)

                                                          aCATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGc            >  1:4283257/1‑71 (MQ=255)

                                                           cATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTc                                         >  1:802483/1‑41 (MQ=255)

                                                           cATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTc                                         <  1:600178/41‑1 (MQ=255)

                                                             tACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTc                                         <  1:1082178/39‑1 (MQ=255)

                                                             tACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTc                                         <  1:396009/39‑1 (MQ=255)

                                                             tACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGc                               <  1:2466572/49‑1 (MQ=255)

                                                             tACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaa                            <  1:5627644/52‑1 (MQ=255)

                                                             tACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaa                            <  1:1697807/52‑1 (MQ=255)

                                                              aCGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCaa        >  1:5118750/1‑71 (MQ=255)

                                                               cGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAAc                         <  1:4641982/53‑1 (MQ=255)

                                                               cGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAAt       >  1:4691553/1‑71 (MQ=255)

                                                               cGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAAt       >  1:2123252/1‑71 (MQ=255)

                                                                gCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaa                            <  1:4283010/49‑1 (MQ=255)

                                                                 cAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAAttt     <  1:2527354/71‑1 (MQ=255)

                                                                 cAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAAttt     <  1:2737867/71‑1 (MQ=255)

                                                                  aGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTc    <  1:5590133/71‑1 (MQ=255)

                                                                  aGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTc    <  1:447287/71‑1 (MQ=255)

                                                                    cTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTctg  <  1:5453895/71‑3 (MQ=255)

                                                                     tGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGa                                 >  1:3548325/1‑39 (MQ=255)

                                                                     tGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGa                                 >  1:3208350/1‑39 (MQ=255)

GACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGG  >  Exported/2570752‑2570890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`