BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	2,570,851	C→T	10.3%	F252F (TTC→TTT)	yibH →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	2,570,851	0	C	T	10.3%	264.8 / 14.8	97	F252F (TTC→TTT)	yibH	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (43/44); new base T (4/6); total (47/50)
Fisher's exact test for biased strand distribution p-value = 7.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

Only 100 of 109 total aligned reads displayed.

CGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAACCTGGC  >  Exported/2570782‑2570920

cGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTc                                                                       <  1:4657486/70‑1 (MQ=255)

cGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCa                                                                      >  1:2203160/1‑71 (MQ=255)

  ttACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATc                                                                    >  1:508147/1‑71 (MQ=255)

    aCTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATcc                                                                   >  1:415778/1‑70 (MQ=255)

        aGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAg                                                              >  1:2878984/1‑71 (MQ=255)

         ggTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAg                                                              >  1:3890918/1‑70 (MQ=255)

          gTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCGGTGATGGTGTTTATACCCGATCa                                                            <  1:2235105/71‑1 (MQ=255)

            aCTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCGGTGATGGTGTTTATACCCGATCag                                                           >  1:5457882/1‑69 (MQ=255)

             cTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCGGTGATGGTGTTTATACCCGATCaga                                                          <  1:1211790/70‑3 (MQ=255)

             cTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaa                                                          <  1:2417283/70‑1 (MQ=255)

             cTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaa                                                          <  1:3172040/70‑1 (MQ=255)

             cTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaa                                                          <  1:1625402/70‑1 (MQ=255)

             cTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaa                                                          <  1:1549848/70‑1 (MQ=255)

               gATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATccc                                                                  >  1:1758223/1‑60 (MQ=255)

               gATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCa                                                            >  1:1108039/1‑66 (MQ=255)

                aTCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAAc                                                       <  1:4349864/70‑1 (MQ=255)

                 tCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCAt                                                                     >  1:2717923/1‑55 (MQ=255)

                 tCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACgg                                                     >  1:4744885/1‑71 (MQ=255)

                   cGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCa                                                   <  1:2159368/71‑1 (MQ=255)

                   cGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCa                                                   <  1:2631997/71‑1 (MQ=255)

                     cccAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCa                                                   <  1:1483664/69‑1 (MQ=255)

                         ggTACATACGCAGCTGCCTTGCCGCTGCGTACGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGt                                             >  1:1046851/1‑71 (MQ=255)

                          gTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaaa                                                         <  1:2733484/58‑1 (MQ=255)

                          gTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTc                                            <  1:1125899/71‑1 (MQ=255)

                            aCATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTc                                                                       >  1:1756262/1‑42 (MQ=255)

                            aCATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCa                                                                      >  1:1705886/1‑43 (MQ=255)

                            aCATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGc                                          >  1:4283257/1‑71 (MQ=255)

                             cATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTc                                                                       <  1:600178/41‑1 (MQ=255)

                             cATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTc                                                                       >  1:802483/1‑41 (MQ=255)

                               tACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTc                                                                       <  1:396009/39‑1 (MQ=255)

                               tACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTc                                                                       <  1:1082178/39‑1 (MQ=255)

                               tACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGc                                                             <  1:2466572/49‑1 (MQ=255)

                               tACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaa                                                          <  1:5627644/52‑1 (MQ=255)

                               tACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaa                                                          <  1:1697807/52‑1 (MQ=255)

                                aCGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCaa                                      >  1:5118750/1‑71 (MQ=255)

                                aCGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCaa                                      >  1:3306124/1‑71 (MQ=255)

                                 cGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAAc                                                       <  1:4641982/53‑1 (MQ=255)

                                 cGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAAt                                     >  1:2123252/1‑71 (MQ=255)

                                 cGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAAt                                     >  1:4691553/1‑71 (MQ=255)

                                   cAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAAttt                                   <  1:2737867/71‑1 (MQ=255)

                                   cAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAAttt                                   >  1:1563921/1‑71 (MQ=255)

                                   cAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAAttt                                   <  1:2527354/71‑1 (MQ=255)

                                    aGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTc                                  <  1:5590133/71‑1 (MQ=255)

                                    aGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTc                                  <  1:447287/71‑1 (MQ=255)

                                      cTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTctg                                <  1:5453895/71‑3 (MQ=255)

                                       tGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGa                                                               >  1:3548325/1‑39 (MQ=255)

                                       tGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGa                                                               >  1:3208350/1‑39 (MQ=255)

                                         ccTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCgg                                >  1:5018281/1‑68 (MQ=255)

                                         ccTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCaa                             <  1:4999601/71‑1 (MQ=255)

                                         ccTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCaa                             <  1:4446137/71‑1 (MQ=255)

                                         ccTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCa                              >  1:364113/1‑70 (MQ=255)

                                          gctGCCGCTACGTCCGGTGATGGTGTTTATACCCGATCAGAAAc                                                       >  1:3872822/3‑44 (MQ=12)

                                          gctGCCGCTACGTCCGGTGATGGTGTTTATACCCGATCAGAAACGACAAATCGTGGCGCAGTTCCGTCag                             <  1:729192/68‑2 (MQ=255)

                                          gctGCCGCTACGTCCGGTGATGGTGTTTATACCCGATCAGAAACGACAAATCGTGGCGCAGTTCCGTCag                             <  1:4087924/68‑2 (MQ=255)

                                            tGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCTAAAACGGCAAATTGTCGCCCAAttt                                   >  1:2746410/1‑62 (MQ=255)

                                            tGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAAttt                                   >  1:1634008/1‑62 (MQ=255)

                                            tGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCaaaa                           >  1:4206615/1‑70 (MQ=255)

                                            tGCCGCTGCGTCCGGTGATGGTCTTCAT‑CCCGAGCAAAAACGGCAAATTGTCGCCCAAttt                                   >  1:1560527/1‑61 (MQ=255)

                                             gCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGt                                             >  1:2903263/1‑51 (MQ=255)

                                             gCCGCTACGTCCGGTGATGGTGTTTATACCCGATCAGAAACGACAAATCGTGGCGCAGTTCCGTCAGAACt                         >  1:1614448/1‑71 (MQ=255)

                                              ccGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTc                        <  1:1531971/71‑1 (MQ=255)

                                              ccGCTACGTCCGGTGATGGTGTTTATACCCGATCAGAAACGACAAATCGTGGCGCAGTTCCGTCAGAACTc                        >  1:352963/1‑71 (MQ=255)

                                               cGCTACGTCCGGTGATGGTGTTTATACCCGATCAGAAACGACAAATCGTGGCGCAGTTCCGTCAGAACTcc                       <  1:1330329/71‑2 (MQ=255)

                                                gCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAAtt                                               <  1:1895200/46‑1 (MQ=255)

                                                gCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGc                      <  1:774436/71‑1 (MQ=255)

                                                 cTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCt                     >  1:3564061/1‑71 (MQ=255)

                                                  tacGTCCGGTGATGGTGTTTATACCCGATCAGAAACGACAAATCGTGGCGCAGTTCCGTCag                             <  1:1854955/60‑2 (MQ=255)

                                                    cGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCg                       <  1:2480910/66‑1 (MQ=255)

                                                    cGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCg                       <  1:4251836/66‑1 (MQ=255)

                                                    cGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCg                       <  1:3251621/66‑1 (MQ=255)

                                                      tCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTAc                <  1:3943/71‑1 (MQ=255)

                                                       ccGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTAc                <  1:5338924/70‑1 (MQ=255)

                                                        cGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGt              >  1:406426/1‑71 (MQ=255)

                                                        cGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGt              >  1:2216233/1‑71 (MQ=255)

                                                        cGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGt              <  1:2256062/71‑1 (MQ=255)

                                                          gTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCt            <  1:2653371/71‑1 (MQ=255)

                                                          gTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCt            <  1:5055378/71‑1 (MQ=255)

                                                           tGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGc                               <  1:2582970/51‑1 (MQ=255)

                                                           tGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCt            >  1:3074010/1‑70 (MQ=255)

                                                           tGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTg           <  1:5162461/71‑1 (MQ=255)

                                                              tGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAAt                                     <  1:1138752/42‑1 (MQ=255)

                                                              tGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGaaa        >  1:3445468/1‑71 (MQ=255)

                                                               ggTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAAc       >  1:852979/1‑71 (MQ=255)

                                                               ggTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAAc       >  1:964811/1‑71 (MQ=255)

                                                               ggTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAAc       >  1:1499124/1‑71 (MQ=255)

                                                                gTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAAcc      <  1:4823359/71‑1 (MQ=255)

                                                                 tCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAACCt     >  1:5240482/1‑71 (MQ=255)

                                                                  cTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAACCTg    >  1:5380065/1‑71 (MQ=255)

                                                                  cTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAACCTg    >  1:1113757/1‑71 (MQ=255)

                                                                   ttCATCCCCGAGCAAAAACGGCAAATTGTCGCCCa                                       <  1:660700/35‑1 (MQ=255)

                                                                   ttCATCCCCGAGCAAAAACGGCAAATTGTCGCCCa                                       <  1:759311/35‑1 (MQ=255)

                                                                   ttCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTCCGGCaa                             <  1:733199/45‑1 (MQ=255)

                                                                    tCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTc             >  1:4280645/1‑60 (MQ=255)

                                                                    tCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTc             >  1:3492808/1‑60 (MQ=255)

                                                                     cATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCaaaa                           >  1:260624/1‑45 (MQ=255)

                                                                     cATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTAc                <  1:3973375/56‑1 (MQ=255)

                                                                     cATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTAc                <  1:359928/56‑1 (MQ=255)

                                                                     cATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAACCTGGc  >  1:4865766/1‑70 (MQ=255)

                                                                     cATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAACCTGGc  >  1:4302455/1‑70 (MQ=255)

                                                                     cATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGACTGAAACCTgg   >  1:2235055/1‑69 (MQ=255)

CGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAACCTGGC  >  Exported/2570782‑2570920

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`