Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,571,121 C→T 13.6% G342G (GGC→GGT)  yibH → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,571,1210CT13.6% 311.0 / 31.7 125G342G (GGC→GGT) yibHhypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (41/67);  new base T (14/3);  total (55/70)
Fisher's exact test for biased strand distribution p-value = 1.03e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.84e-01

Only 100 of 127 total aligned reads displayed.

CCGGCACGGACGGTGTGCTGGGAACCATTGAACTGGACCCTAACGATGATATCGATGCCTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGC  >  Exported/2571051‑2571188
                                                                      |                                                                   
ccGGCACGGACGGTGTGCTGGGAACCATTGAACTGGACCCTAACGATGATATCGATGCCTTACCCGACGGc                                                                     <  1:3488973/71‑1 (MQ=255)
  ggCACGGACGGTGTGCTGGGAACCATTGAACTGGACCCTAACGATGATATCGATGCCTTACCCGACGGCAt                                                                   >  1:4841289/1‑71 (MQ=255)
   gCACGGACGGTGTGCTGGGAACCATTGAACTGGACCCTAACGATGATATCGATGCCTTACCCGACGGCATc                                                                  <  1:4581699/71‑1 (MQ=255)
   gCACGGACGGTGTGCTGGGAACCATTGAACTGGACCCTAACGATGATATCGATGCCTTACCCGACGGCATc                                                                  <  1:3013951/71‑1 (MQ=255)
    cACGGACGGTGTGCTGGGAACCATTGAACTGGACCCTAACGATGATATCGATGCCTTACCCGACGGCATCt                                                                 <  1:810724/71‑1 (MQ=255)
       ggacggTGTGCTGGGAACCATTGAACTGGACCCTAACGATGATATCGATGCCTTACCCGACGGCATc                                                                  <  1:320691/67‑1 (MQ=255)
       ggacggTGTGCTGGGAACCATTGAACTGGACCCTAACGATGATATCGATGCCTTACCCGACGGCATc                                                                  <  1:2226061/67‑1 (MQ=255)
       ggacggTGTGCTGGGAACCATTGAACTGGACCCTAACGATGATATCGATGCCTTACCCGACGGCATc                                                                  <  1:2668148/67‑1 (MQ=255)
       ggacggTGTGCTGGGAACCATTGAACTGGACCCTAACGATGATATCGATGCCTTACCCGACGGCATc                                                                  <  1:3828242/67‑1 (MQ=255)
       ggacggTGTGCTGGGAACCATTGAACTGGACCCTAACGATGATATCGATGCCTTACCCGACGGCATc                                                                  <  1:3972112/67‑1 (MQ=255)
             tgtgCTGGGAACCATTGAACTGGACCCTAACGATGATATCGATGCCTTACCCGACGGCAt                                                                   >  1:25842/1‑60 (MQ=255)
             tgtgCTGGGAACCATTGAACTGGACCCTAACGATGATATCGATGCCTTACCCGACGGCAt                                                                   >  1:5487429/1‑60 (MQ=255)
             tgtgCTGGGAACCATTGAACTGGACCCTAACGATGATATCGATGCCTTACCCGACGGCATCTACGCCCa                                                          <  1:3359755/69‑1 (MQ=255)
             tgtgCTGGGAACCATTGAACTGGACCCTAACGATGATATCGATGCCTTACCCGACGGCATCTACGCCCAgg                                                        >  1:1255654/1‑71 (MQ=255)
               tgCTGGGAACCATTGAACTGGACCCTAACGATGATATCGATGCCTTACCCGACGGCa                                                                    <  1:3621813/57‑1 (MQ=255)
               tgCTGGGAACCATTGAACTGGACCCTAACGATGATATCGATGCCTTACCCGACGGCATCTACGcc                                                            <  1:4639024/65‑1 (MQ=255)
               tgCTGGGAACCATTGAACTGGACCCTAACGATGATATCGATGCCTTACCCGACGGCATCTACGCCCAGGt                                                       <  1:2945067/70‑1 (MQ=255)
                   gggAACCATTGAACTGGACCCTAACGATGATATCGATGCCTTACCCGACGGCATCTACGCCCAGGTGGCgg                                                  >  1:262481/1‑71 (MQ=255)
                     gAACCATTGAACTGGACCCTAACGATGATATCGATGCCTTACCCGACGGCATCTACGCCCAGGTGGCGGtt                                                >  1:211201/1‑71 (MQ=255)
                     gAACCATTGAACTGGACCCTAACGATGATATCGATGCCTTACCCGACGGCATCTACGCCCAGGTGGCGGtt                                                >  1:217817/1‑71 (MQ=255)
                     gAACCATTGAACTGGACCCTAACGATGATATCGATGCCTTACCCGACGGCATCTACGCCCAGGTGGCGGtt                                                <  1:1229629/71‑1 (MQ=255)
                     gAACCATTGAACTGGACCCTAACGATGATATCGATGCCTTACCCGACGGCATCTACGCCCAGGTGGCGGtt                                                <  1:747772/71‑1 (MQ=255)
                     gAACCATTGAACTGGACCCTAACGATGATATCGATGCCTTACCCGACGGCATCTACGCCCAGGTGGCGGtt                                                <  1:1136149/71‑1 (MQ=255)
                     gAACCATTGAACTGGACCCTAACGATGATATCGATGCCTTACCCGACGGCATCTACGCCCAGGTGGCGCtt                                                <  1:5537316/71‑1 (MQ=255)
                      aaCCATTGAACTGGACCCTAACGACGATATCGATGCCTTACCCGACGGCATCTACGCCCAGGTGGCGGtt                                                <  1:4378663/70‑1 (MQ=255)
                        ccATTGAACTGGACCCTAACGATGATATCGATGCCTTACCCGACGGCATCTACGCCCAg                                                         >  1:4850571/1‑59 (MQ=255)
                        ccATTGAACTGGACCCTAACGATGATATCGATGCCTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTAc                                             >  1:2240849/1‑71 (MQ=255)
                           ttGAACTGGACCCTAACGATGATATCGATGCCTTACCCGACGGCATc                                                                  <  1:4433594/47‑1 (MQ=255)
                           ttGAACTGGACCCTAACGATGATATCGATGCCTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTcc                                          <  1:4493236/71‑1 (MQ=255)
                             gAACTGGACCCTAACGATGATATCGATGCCTTACCCGACGGCATCTa                                                                >  1:3791913/1‑47 (MQ=255)
                             gAACTGGACCCTAACGATGATATCGATGCCTTACCCGACGGCATCTACGCCCAgg                                                        >  1:1043643/1‑55 (MQ=255)
                             gAACTGGACCCTAACGATGATATCGATGCCTTACCCGACGGCATCTACGCCCAgg                                                        >  1:2158537/1‑55 (MQ=255)
                             gAACTGGACCCTAACGATGATATCGATGCCTTACCCGACGGCATCTACGCCCAgg                                                        >  1:5199416/1‑55 (MQ=255)
                             gAACTGGACCCTAACGATGATATCGATGCCTTACCCGACGGCATCTACGCCCAgg                                                        >  1:1955201/1‑55 (MQ=255)
                             gAACTGGACCCTAACGATGATATCGATGCCTTACCCGACGGCATCTACGCCCAgg                                                        >  1:5111350/1‑55 (MQ=255)
                              aaCTGGACCCTAACGATGATATCGATGCCTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGAc                                       <  1:2608988/71‑1 (MQ=255)
                                 tGGACCCTAACGATGATATCGATGCCTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTa                                              <  1:1882072/61‑1 (MQ=255)
                                 tGGACCCTAACGATGATATCGATGCCTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACt                                            <  1:5065744/63‑1 (MQ=255)
                                 tGGACCCTAACGATGATATCGATGCCTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCa                                     >  1:4962606/1‑70 (MQ=255)
                                   gACCCTAACGATGATATCGATGCCTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCAttt                                  <  1:2367287/71‑1 (MQ=255)
                                   gACCCTAACGATGATATCGATGCCTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCAttt                                  <  1:3539778/71‑1 (MQ=255)
                                      ccTAACGATGATATCGATGCCTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGAc                                       <  1:3077075/63‑1 (MQ=255)
                                        tAACGATGATATCGATGCCTTACCCGACGGCATCTACGCCCAGGt                                                       >  1:4982549/1‑45 (MQ=255)
                                         aaCGATGATATCGATGCCTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCa                            >  1:880349/1‑71 (MQ=255)
                                          aCGATGATATCGATGCCTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTa                                              >  1:2660279/1‑52 (MQ=255)
                                          aCGATGATATCGATGCCTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTc                                 <  1:53430/65‑1 (MQ=255)
                                          aCGATGATATCGATGCCTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCAt                           >  1:1023888/1‑71 (MQ=255)
                                          aCGATGATATCGATGCCTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCAt                           >  1:589619/1‑71 (MQ=255)
                                          aCGATGATATCGATGCCTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCAt                           >  1:4710826/1‑71 (MQ=255)
                                          aCGATGATATCGATGCCTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCAt                           >  1:18021/1‑71 (MQ=255)
                                          aCGATGATATCGATGCCTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCAt                           >  1:1613540/1‑71 (MQ=255)
                                              tgatATCGATGCCTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGttt                       >  1:4241635/1‑71 (MQ=255)
                                              tgatATCGATGCCTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGttt                       >  1:1975749/1‑71 (MQ=255)
                                                atatCGATGCCTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCa                            >  1:200360/1‑64 (MQ=255)
                                                 tatCGATGCCTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCgg                    <  1:3286039/71‑1 (MQ=255)
                                                 tatCGATGCCTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCg                     <  1:5151667/70‑1 (MQ=255)
                                                  atCGATGCCTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACt                                            <  1:5679794/46‑1 (MQ=255)
                                                   tCGATGCCTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGt                   <  1:3017175/70‑1 (MQ=255)
                                                   tCGATGCCTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGt                   <  1:623872/70‑1 (MQ=255)
                                                     gATGCCTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGAt                >  1:3726211/1‑71 (MQ=255)
                                                     gATGCCTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGAt                >  1:2979381/1‑71 (MQ=255)
                                                     gATGCCTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGAt                >  1:3172167/1‑71 (MQ=255)
                                                     gATGCCTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGAt                >  1:4753995/1‑71 (MQ=255)
                                                     gATGCCTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGAt                <  1:2769342/71‑1 (MQ=255)
                                                      aTGCCTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATg               >  1:5314680/1‑71 (MQ=255)
                                                       tGCCTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGttt                       <  1:1158953/62‑1 (MQ=255)
                                                       tGCCTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGc              <  1:2141511/71‑1 (MQ=255)
                                                         ctttACCAGACGGTATTTACGCCCAGGTGGCGGTTTACTCTGATCATTTCAGCCATGTCTCGGTGATGCg             >  1:2556208/3‑70 (MQ=255)
                                                         ctttACCAGACGGTATTTACGCCCAGGTGGCGGTTTACTCTGATCATTTCAGCCATGTCTCGGTGATGCg             >  1:2597122/3‑70 (MQ=255)
                                                         ctttACCAGACGGTATTTACGACCAGGTGGCGGTTTACTCTGATCATTTCAGCCATGTCTCGGTGATGCg             >  1:4595646/3‑70 (MQ=255)
                                                          cTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGa           <  1:1555635/71‑1 (MQ=255)
                                                          cTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGa           <  1:3091865/71‑1 (MQ=255)
                                                          cTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGa           <  1:442177/71‑1 (MQ=255)
                                                          cTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGa           <  1:4500377/71‑1 (MQ=255)
                                                          cTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGa           <  1:1087588/71‑1 (MQ=255)
                                                          cTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGa           <  1:2489517/71‑1 (MQ=255)
                                                          cTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGa           <  1:3007160/71‑1 (MQ=255)
                                                          cTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGa           <  1:4756545/71‑1 (MQ=255)
                                                          cTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGa           <  1:2265140/71‑1 (MQ=255)
                                                          cTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGa           <  1:1480555/71‑1 (MQ=255)
                                                          cTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGa           <  1:2004031/71‑1 (MQ=255)
                                                          cTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGa           <  1:3948811/71‑1 (MQ=255)
                                                           ttACCAGACGGTATTTACGCCCAGGTGGCGGTTTACTCTGATCATTTCAGCCATGTCTCGGTGATGCgca           >  1:3570946/1‑68 (MQ=255)
                                                           ttACCAGACGGTATTTACGCCCAGGTGGCGGTTTACTCTGATCATTTCAGCCATGTCTCGGTGATGCgca           >  1:61714/1‑68 (MQ=255)
                                                            tACCAGACGGTATTTACGCCCAGGTGGCGGTTTACTCTGATCATTTCAGCCATGTCTCGGTGATGCgca           <  1:2708292/69‑3 (MQ=255)
                                                            tACCAGACGGTATTTACGCCCAGGTGGCGGTTTACTCTGATCATTTCAGCCATGTCTCGGTGATGCgca           >  1:2442065/1‑67 (MQ=255)
                                                             aCCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAg        >  1:4422962/1‑71 (MQ=255)
                                                              ccagACGGTATTTACGCCCAGGTGGCGGTTTACTCTGATCATTTCAGCCATGTCTCGGTGAt                >  1:2823070/4‑62 (MQ=255)
                                                              ccagACGGTATTTACGCCCAGGTGGCGGTTTACTCTGATCATTTCAGCCATGTCTCGGTGAt                >  1:3328451/4‑62 (MQ=255)
                                                              ccagACGGTATTTACGCCCAGGTGGCGGTTTACTCTGATCATTTCAGCCATGTCTCGGTGAt                >  1:3602603/4‑62 (MQ=255)
                                                               ccGACGGCATCTACGCCCAGGTGTCGGTTTACTCCGACCATTTCAGCCATGTTTCGGt                   <  1:3640913/58‑1 (MQ=255)
                                                               ccGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGt                   <  1:3453689/58‑1 (MQ=255)
                                                               ccGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGt                   <  1:101163/58‑1 (MQ=255)
                                                               ccGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGt                   <  1:4319024/58‑1 (MQ=255)
                                                               ccGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGt                   <  1:4489800/58‑1 (MQ=255)
                                                                cGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGtgc     >  1:3225277/1‑71 (MQ=255)
                                                                agACGGTATTTACGCCCAGGTGGCGGTTTACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCAAAGtgc     <  1:1571938/70‑1 (MQ=255)
                                                                 gACGGTATTTACGCCCAGGTGGCGGTTTACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCAAAGtgc     >  1:2934453/1‑70 (MQ=255)
                                                                 gACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCAGAAAGtgc     <  1:1849558/70‑1 (MQ=255)
                                                                   cGGTATTTACGCCCAGGTGGCGGTTTACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCAAAGtgctat  >  1:5093293/1‑69 (MQ=255)
                                                                      |                                                                   
CCGGCACGGACGGTGTGCTGGGAACCATTGAACTGGACCCTAACGATGATATCGATGCCTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGC  >  Exported/2571051‑2571188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑