BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	2,571,191	A→C	23.6%	R366R (AGA→CGA) ‡	yibH →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	2,571,191	0	A	C	23.6%	230.4 / 108.5	161	R366R (AGA→CGA) ‡	yibH	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (56/67); new base C (20/18); total (76/85)
Fisher's exact test for biased strand distribution p-value = 4.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 163 total aligned reads displayed.

ATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTGAGGGGGAGGGGGGAGATTATGGAACTT  >  Exported/2571122‑2571258

aTCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAg                                                                    <  1:247959/71‑1 (MQ=255)

 tCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAg                                                                    >  1:3844818/1‑70 (MQ=255)

  cTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGaa                                                                  >  1:5194102/1‑71 (MQ=255)

   tACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGTGGAAAGTGCTGCTAAGAAt                                                                 >  1:5332413/1‑71 (MQ=255)

     cGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGGTAAGaa                                                                  <  1:10340/68‑1 (MQ=255)

     cGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGaa                                                                  <  1:4466867/68‑1 (MQ=255)

     cGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGaa                                                                  <  1:5279253/68‑1 (MQ=255)

     cGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGa                                                                   <  1:3103857/67‑1 (MQ=255)

     cGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGa                                                               >  1:4704239/1‑71 (MQ=255)

       cccAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGAcc                                                             <  1:5510290/71‑1 (MQ=255)

           ggTGGCGGTTTACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCt                                                         <  1:2763793/71‑1 (MQ=255)

           ggTGGCGGTTTACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCt                                                         >  1:622532/1‑71 (MQ=255)

            gTGGCGGTTTACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTg                                                        <  1:3993103/71‑1 (MQ=255)

            gTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTg                                                        <  1:252901/71‑1 (MQ=255)

              ggCGGTTTACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTggg                                                      >  1:1178487/1‑70 (MQ=255)

              ggCGGTTTACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTgg                                                       >  1:4524765/1‑70 (MQ=255)

              ggCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGa                                                      >  1:3191083/1‑71 (MQ=255)

                 ggTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGc                                                   <  1:1867771/71‑1 (MQ=255)

                 ggTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGc                                                   <  1:740455/71‑1 (MQ=255)

                   tttACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCAt                                                 <  1:1072992/71‑1 (MQ=255)

                   tttACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCAt                                                 >  1:1102715/1‑71 (MQ=255)

                    ttACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCt                                                         >  1:4916584/1‑62 (MQ=255)

                    ttACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCt                                                         >  1:978845/1‑62 (MQ=255)

                    ttACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCAt                                                 >  1:2106060/1‑70 (MQ=255)

                    ttACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCAt                                                 >  1:4781569/1‑70 (MQ=255)

                    ttACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCAt                                                 >  1:5544141/1‑70 (MQ=255)

                     tACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTgg                                                       >  1:5479730/1‑63 (MQ=255)

                     tACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCAtt                                                <  1:3043775/70‑1 (MQ=255)

                     tACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCAtt                                                <  1:5006292/70‑1 (MQ=255)

                      aCTCTGATCATTTCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGCATTac                                              >  1:478737/1‑70 (MQ=255)

                      aCTCTGATCATTTCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGCATTac                                              >  1:4997278/1‑70 (MQ=255)

                      aCTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTAt                                              >  1:682438/1‑71 (MQ=255)

                         ccGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCt                                            >  1:5092416/1‑70 (MQ=255)

                          cGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGc                                                          >  1:3770922/1‑55 (MQ=255)

                           gACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTa                                         <  1:3099256/71‑1 (MQ=255)

                            atcATTTCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGCACTACCttt                                          <  1:1088495/67‑1 (MQ=255)

                            aCCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGa                                                               >  1:589001/1‑48 (MQ=255)

                            aCCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGa                                                               >  1:5036744/1‑48 (MQ=255)

                             tcATTTCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTg                                                    <  1:1535765/57‑1 (MQ=255)

                             ccATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTAtt                                       >  1:4091093/1‑71 (MQ=255)

                             ccATTTCAGCCATGTTTCGGGGATGCGGAAAGTGCTGCTAAGAATGACCAGc                                                          <  1:2560631/52‑1 (MQ=255)

                              cATTTCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGAcc                                                             >  1:1493427/1‑48 (MQ=255)

                               aTTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTg                                                        <  1:258812/52‑1 (MQ=255)

                               aTTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTg                                     <  1:1718755/71‑1 (MQ=255)

                               aTTTCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGCATTACCTTTaac                                       >  1:5161912/1‑67 (MQ=255)

                               aTTTCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGCATTACCTTTAtctc                                     >  1:1381078/1‑68 (MQ=255)

                                tttCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGaa                                                                  >  1:3620312/1‑41 (MQ=255)

                                tttCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGaa                                                                  >  1:4178161/1‑41 (MQ=255)

                                tttCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGa                                                      >  1:1763955/1‑53 (MQ=255)

                                tttCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGa                                                      >  1:5674979/1‑53 (MQ=255)

                                tttCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGa                                                      >  1:2274517/1‑53 (MQ=255)

                                tttCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTg                                     <  1:4417888/70‑1 (MQ=255)

                                tttCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCa                                                            <  1:3494986/47‑1 (MQ=255)

                                  tCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGCATTACCTTTATCTCGAt                                  >  1:5009621/1‑71 (MQ=255)

                                   cAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGAt                                  <  1:956006/70‑1 (MQ=255)

                                    aGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGCATTACCTTTATCTCGATCa                                <  1:1321618/71‑1 (MQ=255)

                                    aGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGCATTACCTTTATCTCGATCa                                <  1:2616552/71‑1 (MQ=255)

                                      ccATGTTTCGGTGATGCGGAAATTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCAtt                              <  1:4346763/71‑1 (MQ=255)

                                      ccATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTAtt                                       <  1:2496535/62‑1 (MQ=255)

                                      ccATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTAtt                                       <  1:3158190/62‑1 (MQ=255)

                                      ccATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCAtt                              <  1:5384325/71‑1 (MQ=255)

                                      ccATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCAtt                              <  1:1570369/71‑1 (MQ=255)

                                       cATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTg                                                        <  1:3886935/44‑1 (MQ=255)

                                       cATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGCATTACCTTTATCTCGATCAtta                             <  1:2087464/71‑2 (MQ=255)

                                        aTGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTg                                                        <  1:4080131/43‑1 (MQ=255)

                                        aTGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCAtt                              >  1:603299/1‑69 (MQ=255)

                                        aTGTTTCGGTGATGCGGAAAGTCCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTg                                     >  1:1980416/1‑62 (MQ=255)

                                         tGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTgg                                    <  1:353479/62‑1 (MQ=255)

                                         tGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTgg                                    <  1:4604329/62‑1 (MQ=255)

                                         tGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgag                           <  1:3632032/71‑1 (MQ=255)

                                         tGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGCATTACCTTTATCTCGATCAttaa                            <  1:5176130/70‑3 (MQ=255)

                                          gTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGAcc                                                             <  1:2406125/36‑1 (MQ=255)

                                          gTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTga                            >  1:2894390/1‑69 (MQ=255)

                                          gTTTAGGTGATGCGGAAAGTGCTGCTAAGAATGAcc                                                             <  1:2705178/36‑1 (MQ=255)

                                            ttCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTg                                     >  1:924756/1‑58 (MQ=255)

                                            ttCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTg                                     >  1:4929438/1‑58 (MQ=255)

                                             tCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGa                                                      >  1:2804004/1‑40 (MQ=255)

                                             tCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTga                            >  1:4006483/1‑66 (MQ=255)

                                             tCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGCAtt                                                >  1:875924/1‑46 (MQ=255)

                                             tCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGCAtt                                                >  1:1497352/1‑46 (MQ=255)

                                              cGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCt                                            <  1:5112401/49‑1 (MQ=255)

                                              cGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgaggggg                       <  1:3658348/70‑1 (MQ=255)

                                              cGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgaggggg                       <  1:5288122/70‑1 (MQ=255)

                                              cGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgagggg                        >  1:4349559/1‑69 (MQ=255)

                                               ggTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTg                                                        <  1:2341009/36‑1 (MQ=255)

                                               ggTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgaggggga                      <  1:42438/70‑1 (MQ=255)

                                                   aTGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGAt                                  <  1:4818097/54‑1 (MQ=255)

                                                   aTGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGAt                                  <  1:3269735/54‑1 (MQ=255)

                                                   aTGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGAt                                  <  1:1691149/54‑1 (MQ=255)

                                                   aTGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCAtt                              <  1:2620025/58‑1 (MQ=255)

                                                    tGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGCATTACCTTTATCTCGATCAtta                             <  1:4467704/58‑2 (MQ=255)

                                                     gcgcAAAGTGCTATTACGCATGACCAGCTGGGTGCAt                                                 >  1:3719673/1‑37 (MQ=255)

                                                     gCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTAttt                                      <  1:5496116/48‑1 (MQ=255)

                                                       ggAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTga                            <  1:4302414/56‑1 (MQ=255)

                                                         aaaGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTGAGGGGGAGGGGGGAGAt            >  1:616329/1‑70 (MQ=255)

                                                          aaGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTGAGGGGGAGGGGGGAGATTa          >  1:2923462/1‑71 (MQ=255)

                                                             tgctgcTAAGAATGACCAGCTGGATGCATTATCttt                                          <  1:5405463/36‑1 (MQ=255)

                                                               ctgctAAGAATGACCAGCTGGATGCATTATCTTTa                                         >  1:5485272/1‑35 (MQ=255)

                                                               ctgctAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTGAGGGGGAGGGGGGAGATTATGGaa     >  1:2258770/1‑71 (MQ=255)

                                                                 gctAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCa                                <  1:724465/42‑1 (MQ=255)

ATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTGAGGGGGAGGGGGGAGATTATGGAACTT  >  Exported/2571122‑2571258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`