Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,571,330 T→C 29.2% intergenic (+98/‑1681) yibH → / → ysaB hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,571,3300TC29.2% 193.7 / 99.2 137intergenic (+98/‑1681)yibH/ysaBhypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (46/51);  new base C (21/19);  total (67/70)
Fisher's exact test for biased strand distribution p-value = 7.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01

Only 100 of 143 total aligned reads displayed.

CTCCTTGTAAGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATG  >  Exported/2571261‑2571398
                                                                      |                                                                      
  ccTTGTAAGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATg                                                                     >  1:2843252/1‑71 (MQ=255)
     tGTAAGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGt                                                                    >  1:4428264/1‑69 (MQ=255)
             cTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTc                                                          >  1:901165/1‑71 (MQ=255)
             cTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTc                                                          <  1:4524869/71‑1 (MQ=255)
               aaTTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTg                                                      >  1:2212674/1‑71 (MQ=255)
                attattTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATg                                                                     >  1:486374/1‑57 (MQ=255)
                attattTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTg                                                      <  1:1810774/70‑1 (MQ=255)
                attattTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTg                                                      <  1:4295718/70‑1 (MQ=255)
                 ttattTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGt                                                            <  1:4140373/65‑1 (MQ=255)
                 ttattTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGt                                                            <  1:1623633/65‑1 (MQ=255)
                  tattTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGAt                                                   >  1:1101002/1‑71 (MQ=255)
                   attTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGAt                                                   >  1:2657581/1‑70 (MQ=255)
                   attTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGAt                                                   >  1:2151432/1‑70 (MQ=255)
                    tttttGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGAt                                                   >  1:3723745/1‑69 (MQ=255)
                            tatTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGctc                                         >  1:2722097/1‑71 (MQ=255)
                             atTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTg                                                      <  1:3553326/57‑1 (MQ=255)
                             atTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTg                                                      <  1:3364244/57‑1 (MQ=255)
                             atTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGa                                                    >  1:4371958/1‑59 (MQ=255)
                             atTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGctct                                        >  1:4021442/1‑71 (MQ=255)
                             atTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGctct                                        >  1:5669681/1‑71 (MQ=255)
                              ttACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTtaataa                                                              >  1:4959213/1‑50 (MQ=255)
                              ttACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCtctc                                       <  1:2285486/71‑1 (MQ=255)
                                 cTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTTTGTTAATAAGTTc                                                          <  1:687733/51‑1 (MQ=255)
                                 cTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTgca                                                    >  1:5408570/1‑53 (MQ=255)
                                  ttCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGt                                                            <  1:5675227/48‑1 (MQ=255)
                                  ttCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTg                                                      <  1:284437/52‑1 (MQ=255)
                                  ttCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTg                                                      <  1:1216093/52‑1 (MQ=255)
                                    cATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGa                                                 <  1:1926925/55‑1 (MQ=255)
                                    cATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGa                                 >  1:2016698/1‑71 (MQ=255)
                                     aTTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGAt                                <  1:327776/71‑1 (MQ=255)
                                      ttAGCTTCGATGATTTTTCTTTAATATTCATTATGtt                                                                   >  1:5005218/1‑37 (MQ=255)
                                      ttAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGAtt                               >  1:598891/1‑71 (MQ=255)
                                       tAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGTAtt                               <  1:225473/70‑1 (MQ=255)
                                        aGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGAttt                              <  1:2451652/70‑1 (MQ=255)
                                             tgatgatTTTTCCTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGAtttctt                           <  1:4252406/69‑1 (MQ=255)
                                             tgatgatTTTTCCTTTAATATCCAGCATGTTAAGAAGTTCt                                                        <  1:4702250/40‑1 (MQ=12)
                                             cGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTc                                                          <  1:4416152/39‑1 (MQ=255)
                                             cGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCt                                                        >  1:3069491/1‑40 (MQ=255)
                                             cGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCt                                                        >  1:4491163/1‑40 (MQ=255)
                                              gatgatTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATcc                       <  1:1022779/71‑1 (MQ=255)
                                               atgatTTTTCCTTTAATATCCAGCATGTTaagaag                                                             >  1:1241167/1‑35 (MQ=9)
                                               atgatTTTTCCTTTAATATCCAGCATGTTaagaag                                                             >  1:4082362/1‑35 (MQ=9)
                                               atgatTTTTCCTTTAATATCCAGCATGTTAAGAAGTTc                                                          <  1:2605981/38‑1 (MQ=12)
                                               atgatTTTTCCTTTAATATCCAGCATGTTAAGAAGTTc                                                          <  1:3842416/38‑1 (MQ=12)
                                               atgatTTTTCCTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATc                        >  1:3425292/1‑71 (MQ=255)
                                               atgatTTTTCCTTTAATATCCAGCATGTTAAGAAGTTCTgg                                                     >  1:1356806/1‑41 (MQ=16)
                                               atgatTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCAtt                                    <  1:1042987/57‑1 (MQ=255)
                                                 gatTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATcc                       <  1:3671395/68‑1 (MQ=255)
                                                 gatTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTc                    >  1:3264253/1‑71 (MQ=255)
                                                 gatTTTACTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTc                    >  1:734041/1‑71 (MQ=255)
                                                 gatATTTCTTTAATATTCATTATGTTAATAAGTTCTg                                                      >  1:5530527/1‑37 (MQ=255)
                                                  tttttcCTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTc                    >  1:5093691/2‑71 (MQ=255)
                                                   tttttCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGtgtg                                            <  1:1745162/45‑1 (MQ=255)
                                                   tttttCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGtgtg                                            <  1:720328/45‑1 (MQ=255)
                                                   tttttCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGtgtg                                            <  1:4008930/45‑1 (MQ=255)
                                                   tttttCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCtt                  <  1:119029/71‑1 (MQ=255)
                                                    tttcCTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCtt                  >  1:2110719/1‑71 (MQ=255)
                                                     tttCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTg                 <  1:4212067/70‑1 (MQ=255)
                                                     tttCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTg                 <  1:3487164/70‑1 (MQ=255)
                                                      ttccTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCtt                  <  1:1754179/67‑1 (MQ=255)
                                                       tccTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTg                 <  1:2989061/68‑1 (MQ=255)
                                                       tccTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTg                 <  1:4726710/68‑1 (MQ=255)
                                                       tccTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTg                 <  1:529762/68‑1 (MQ=255)
                                                       tccTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTg                 <  1:2353435/68‑1 (MQ=255)
                                                       tccTTTAATATCCAGCATGTTAAGAAGTTCTGGGATAGCTGTAATCTCATTAGATTTCTCATCCGTCTTg                 <  1:4254032/68‑1 (MQ=255)
                                                       tccTTTAATATCCAGCATGTTAAGAAGTTCTGGGATAGCTGTAATCTCATTAGATTTCTCATCCGTCTTg                 <  1:4309757/68‑1 (MQ=255)
                                                       tccTTTAATATCCAGCATGTTAAGAAGTTCTGGGATAGCTGTAATCTCATTAGATTTCTCATCCGTCTTg                 <  1:1522883/68‑1 (MQ=255)
                                                       tccTTTAATATCCAGCATGTTAAGAAGTTCTGGGATAGCTGTAATCTCATTAGATTTCTCATCCGTCTTg                 <  1:2759612/68‑1 (MQ=255)
                                                       tCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGtt               <  1:5695919/70‑1 (MQ=255)
                                                         cTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGtt               <  1:5031128/69‑1 (MQ=255)
                                                         cTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCt             >  1:4191709/1‑71 (MQ=255)
                                                         cTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTGATc              >  1:1524999/1‑71 (MQ=255)
                                                         cTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTGATc              <  1:116806/71‑1 (MQ=255)
                                                         cTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTGATc              >  1:4842888/1‑71 (MQ=255)
                                                          tttAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTg                 <  1:5480766/67‑1 (MQ=255)
                                                            tAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGcc          >  1:837254/1‑71 (MQ=255)
                                                            tAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGcc          >  1:109743/1‑71 (MQ=255)
                                                            tAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTa                          >  1:4814237/1‑56 (MQ=255)
                                                            tAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTa                          >  1:2221760/1‑56 (MQ=255)
                                                            tAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTGATCt             >  1:3759709/1‑69 (MQ=255)
                                                            tAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTGATCTGc           >  1:5528958/1‑71 (MQ=255)
                                                            tAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTGATCTGc           >  1:3672754/1‑71 (MQ=255)
                                                            tAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTGATCTGc           >  1:2356605/1‑71 (MQ=255)
                                                            tAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTGATCTGc           >  1:4743825/1‑71 (MQ=255)
                                                             aaTATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCt             <  1:2164482/67‑1 (MQ=255)
                                                              atatTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCg         <  1:1877765/70‑1 (MQ=255)
                                                                 ttCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATc                        >  1:5095556/1‑52 (MQ=255)
                                                                 ttCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATc                        >  1:4038816/1‑52 (MQ=255)
                                                                 ttCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCt             <  1:1611576/63‑1 (MQ=255)
                                                                 ttCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAgt     <  1:5305193/71‑2 (MQ=255)
                                                                 ttCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAg      <  1:1864496/70‑1 (MQ=255)
                                                                 ttCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAg      <  1:3328060/70‑1 (MQ=255)
                                                                   cATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGAt   >  1:2803109/1‑71 (MQ=255)
                                                                    aTTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATcc                       >  1:482338/1‑50 (MQ=255)
                                                                    aTTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGAt   <  1:3930766/70‑1 (MQ=255)
                                                                    aTTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATg  <  1:4985725/71‑1 (MQ=255)
                                                                    aTTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTATATCCGTCTTGTTCTGCCGCAGGAt   <  1:5026670/70‑1 (MQ=255)
                                                                     ttATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTa                          <  1:398453/46‑1 (MQ=255)
                                                                     ttATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGtt               <  1:127074/57‑1 (MQ=255)
                                                                      tATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGAt   >  1:5231582/1‑68 (MQ=255)
                                                                      |                                                                      
CTCCTTGTAAGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATG  >  Exported/2571261‑2571398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: