Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,571,397 T→C 18.0% intergenic (+165/‑1614) yibH → / → ysaB hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,571,3970TC18.0% 320.1 / 59.2 150intergenic (+165/‑1614)yibH/ysaBhypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (48/75);  new base C (15/12);  total (63/87)
Fisher's exact test for biased strand distribution p-value = 1.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01

Only 100 of 163 total aligned reads displayed.

CATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAA  >  Exported/2571327‑2571465
                                                                      |                                                                    
cATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGAt                                                                      >  1:2803109/1‑71 (MQ=255)
cATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGAt                                                                      >  1:1549294/1‑71 (MQ=255)
 aTTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGAt                                                                      <  1:3930766/70‑1 (MQ=255)
 aTTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATg                                                                     <  1:4985725/71‑1 (MQ=255)
       ttAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACta                                                               <  1:1616543/71‑1 (MQ=255)
       ttAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACt                                                                <  1:596909/70‑1 (MQ=255)
        taataaGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACta                                                               >  1:4223154/1‑70 (MQ=255)
          ataaGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACtata                                                             >  1:2060905/1‑70 (MQ=255)
            aaGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATga                                                                    >  1:246516/1‑61 (MQ=255)
              gTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGAc                                                                 <  1:153790/62‑1 (MQ=255)
                 cTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACAtcgtc                                                      <  1:5004618/70‑1 (MQ=255)
                 cTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACAtcgtc                                                      <  1:5097885/70‑1 (MQ=255)
                 cTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACAtcgtc                                                      <  1:3511341/70‑1 (MQ=255)
                  tGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACAtcgtc                                                      <  1:3932380/69‑1 (MQ=255)
                    gATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGAt                                                                      >  1:1823475/1‑51 (MQ=255)
                    gATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGAt                                                                      >  1:2566562/1‑51 (MQ=255)
                    gATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACAtcgtc                                                      <  1:5436525/67‑1 (MQ=255)
                    gATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCgaga                                                  <  1:5619635/71‑1 (MQ=255)
                    gATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCgag                                                   <  1:3413843/70‑1 (MQ=255)
                    gATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCga                                                    <  1:4149641/69‑1 (MQ=255)
                    gATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCga                                                    <  1:666272/69‑1 (MQ=255)
                    gATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCga                                                    <  1:4358569/69‑1 (MQ=255)
                    gATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGACGCAGGATGAGACTATACATCGTCgag                                                   <  1:3829152/70‑1 (MQ=255)
                      tGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACa                                                           <  1:1392556/60‑1 (MQ=255)
                       gAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCg                                               >  1:463623/1‑71 (MQ=255)
                       gAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCg                                               >  1:1537116/1‑71 (MQ=255)
                       gAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCg                                               >  1:3667859/1‑71 (MQ=255)
                            tgCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatg                                          <  1:4960781/71‑1 (MQ=255)
                             gCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCgag                                                   <  1:4165716/61‑1 (MQ=255)
                              ctctCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatgat                                        <  1:151787/71‑1 (MQ=255)
                              atctcATTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACg                                               <  1:1855778/63‑1 (MQ=255)
                              atctcATTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACg                                               <  1:4993226/63‑1 (MQ=255)
                               tctcATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACtata                                                             >  1:2847201/1‑49 (MQ=255)
                               tctcATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACtata                                                             >  1:814179/1‑49 (MQ=255)
                                ctcATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATga                                                                    >  1:3864690/1‑41 (MQ=255)
                                ctcATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATga                                                                    >  1:2578423/1‑41 (MQ=255)
                                 tcATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCgaga                                                  >  1:4175455/1‑58 (MQ=255)
                                 tcATTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCacta                                          <  1:1135986/66‑4 (MQ=255)
                                 tcATTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCacta                                          <  1:4315466/66‑4 (MQ=255)
                                  cATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACtata                                                             >  1:510010/1‑46 (MQ=255)
                                  cATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACtata                                                             >  1:1435367/1‑46 (MQ=255)
                                  cATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACtata                                                             >  1:1706652/1‑46 (MQ=255)
                                  cATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatgatg                                       <  1:1937277/68‑1 (MQ=255)
                                  cATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGata                                    >  1:1951030/1‑71 (MQ=255)
                                   aTTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAAtgatga                                      <  1:552476/68‑1 (MQ=255)
                                   aTTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAAtgatga                                      <  1:3581123/68‑1 (MQ=255)
                                   aTTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGatat                                   <  1:3135749/71‑1 (MQ=255)
                                   aTTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTgagaa                                                 >  1:1460628/1‑56 (MQ=255)
                                   aTTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCActaatg                                       <  1:4527043/67‑1 (MQ=255)
                                   aTTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCACtaatgaca                                    <  1:4734283/70‑3 (MQ=255)
                                   aTTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCACTAATGacat                                   >  1:1715371/1‑71 (MQ=255)
                                   aTTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCACTAATGacat                                   >  1:2753796/1‑71 (MQ=255)
                                   aTTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCACTAATGacat                                   >  1:1508719/1‑71 (MQ=255)
                                    ttGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATga                                                                    <  1:4703125/37‑1 (MQ=255)
                                    ttGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATga                                                                    <  1:3939917/37‑1 (MQ=255)
                                    ttGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCa                                             <  1:1107040/60‑1 (MQ=255)
                                    ttGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAat                                           <  1:5173566/62‑1 (MQ=255)
                                     tGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGatat                                   >  1:5176006/1‑69 (MQ=255)
                                      ggATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatga                                         >  1:4112445/1‑62 (MQ=255)
                                      ggATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAg                                >  1:4664338/1‑71 (MQ=255)
                                      ggATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTTTGCATTGTTGAGAACGCACTAATGACATGa                                 <  1:5040939/70‑1 (MQ=255)
                                      ggATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCACTAATGACATGa                                 <  1:947627/70‑1 (MQ=255)
                                       gATTTCTTATCCGTCTTGATCTGTCCGATGACCAGACTGTGCATTGTTGAGAACGCACTAATGACATGaat                               >  1:3122013/1‑69 (MQ=255)
                                         ttttttATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATg                                  <  1:231130/66‑1 (MQ=255)
                                          tttttATCCGTCTTGTTCTGCCGCAGGATGAGACTATACAtcgtcg                                                     <  1:1363715/46‑1 (MQ=255)
                                          tttttATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatgatg                                       >  1:2172702/1‑60 (MQ=255)
                                            tttATCGGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCaa                                            >  1:731348/1‑53 (MQ=255)
                                            tttATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTtc                          >  1:2823603/1‑71 (MQ=255)
                                              tATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTtcc                         <  1:2406726/70‑1 (MQ=255)
                                                tCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAAtgatga                                      <  1:3553576/55‑1 (MQ=255)
                                                tCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAAtgatga                                      <  1:5384567/55‑1 (MQ=255)
                                                tCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTtcctcc                      >  1:2529112/1‑71 (MQ=255)
                                                  cGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTcctcct                     <  1:5342248/70‑1 (MQ=255)
                                                  cGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTg                    >  1:5491644/1‑71 (MQ=255)
                                                  cGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTg                    >  1:3330444/1‑71 (MQ=255)
                                                    tCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTg                    <  1:3229031/69‑1 (MQ=255)
                                                      ttGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGAc                >  1:1955615/1‑71 (MQ=255)
                                                      ttGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGAc                >  1:1260997/1‑71 (MQ=255)
                                                      ttGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGAATGATGATATGAGTTGTTCCTCCTGCGACg               >  1:1625714/1‑71 (MQ=255)
                                                      ttGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGAATGATGATATGAGTTGTTCCTCCTGCGACg               >  1:1619006/1‑71 (MQ=255)
                                                        gTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGAc                <  1:1230706/69‑1 (MQ=255)
                                                         ttCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAAtgatga                                      <  1:4301107/46‑1 (MQ=255)
                                                         ttCTGCCCCAGGATGAGACTATACATCGTCGAGATCGCAAtgatga                                      >  1:4292406/1‑46 (MQ=255)
                                                          tCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCa                                             >  1:813073/1‑38 (MQ=255)
                                                           cTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAAtgatga                                      <  1:1971366/44‑1 (MQ=255)
                                                           cTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGctc           >  1:4362226/1‑71 (MQ=255)
                                                           cTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGct            >  1:3810355/1‑70 (MQ=255)
                                                            tGCCGCAGGATGAGACTATACATCGTCGAGATCGCAat                                           <  1:64923/38‑1 (MQ=255)
                                                              ccGCAGGATGAGACTATACATCGTCGAGATCGCAatga                                         >  1:2298830/1‑38 (MQ=255)
                                                              ccGCAGGATGAGACTATACATCGTCGAGATCGCAatga                                         >  1:4033043/1‑38 (MQ=255)
                                                              ccGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGctct          <  1:482278/69‑1 (MQ=255)
                                                               cGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCg                  >  1:3029933/1‑60 (MQ=255)
                                                                 cAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCa     <  1:4786608/71‑1 (MQ=255)
                                                                  aGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGAc                <  1:3721742/59‑1 (MQ=255)
                                                                    gATGAGACTATACATCGTCGAGATCGCAATGATGATATGAgtt                              <  1:2142377/43‑1 (MQ=255)
                                                                    gATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTa         <  1:5339379/64‑1 (MQ=255)
                                                                    gATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCaaaa  <  1:3290580/71‑1 (MQ=255)
                                                                     aTGAGACTATACATCGTCGAGATCGCAATGATGATATGAgtt                              <  1:4218532/42‑1 (MQ=255)
                                                                      tGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTaa        >  1:5210211/1‑63 (MQ=255)
                                                                      tGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCaaaa  >  1:2546351/1‑69 (MQ=255)
                                                                      |                                                                    
CATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAA  >  Exported/2571327‑2571465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: