| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,571,425 | G→A | 9.7% | intergenic (+193/‑1586) | yibH → / → ysaB | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,571,425 | 0 | G | A | 9.7% | 252.2 / 12.1 | 93 | intergenic (+193/‑1586) | yibH/ysaB | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (52/31); new base A (6/3); total (58/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 128 total aligned reads displayed.
TGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAAGAGCAGGAGTGCTTTTCCATCTATTGC > Exported/2571355‑2571494 | tgCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatg < 1:3810209/71‑1 (MQ=255)tgCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatg < 1:1268388/71‑1 (MQ=255)tgCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatg < 1:4960781/71‑1 (MQ=255) ctcATTAGATTTCTCATCCGTCTTGATCTGTCCGATGACCAGACTGTGCATTGTTGAGAACGCACtaatga > 1:2317238/1‑71 (MQ=255) ctcATTAGATTTCTCATCCGTCTTGATCTGTCCGATGACCAGACTGTGCATTGTTGAGAACGCACtaatga > 1:1943869/1‑71 (MQ=255) tcATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAAtgatga > 1:4314960/1‑70 (MQ=255) cATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatgatg < 1:3904393/68‑1 (MQ=255) cATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatgatg < 1:1937277/68‑1 (MQ=255) aTTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAAtgatga < 1:3581123/68‑1 (MQ=255) aTTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGatat < 1:3135749/71‑1 (MQ=255) aTTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCActaatg < 1:4527043/67‑1 (MQ=255) aTTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCACtaatgaca < 1:4734283/70‑3 (MQ=255) aTTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCACTAATGacat > 1:1508719/1‑71 (MQ=255) aTTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCACTAATGacat > 1:2753796/1‑71 (MQ=255) aTTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCACTAATGacat > 1:1715371/1‑71 (MQ=255) aTTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCACTAATGacat > 1:3556466/1‑71 (MQ=255) tGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGatat > 1:5176006/1‑69 (MQ=255) ggATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatgat < 1:5399116/63‑1 (MQ=255) ggATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAg > 1:4664338/1‑71 (MQ=255) ggATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAg > 1:2577682/1‑71 (MQ=255) ggATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTTTGCATTGTTGAGAACGCACTAATGACATGa < 1:5040939/70‑1 (MQ=255) ggATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCACTAATGACATGa < 1:1182047/70‑1 (MQ=255) ggATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCACTAATGACATGa < 1:947627/70‑1 (MQ=255) gATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAgt > 1:383292/1‑71 (MQ=255) gATTTCTTATCCGTCTTGATCTGTCCGATGACCAGACTGTGCATTGTTGAGAACGCACTAATGACATGaat > 1:3122013/1‑69 (MQ=255) ttttttATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATg < 1:2994731/66‑1 (MQ=255) tttttATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatgatg > 1:3515198/1‑60 (MQ=255) tttttATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatgatg > 1:2172702/1‑60 (MQ=255) ttttATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAgttgtt > 1:622813/1‑71 (MQ=255) tttATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTtc > 1:2823603/1‑71 (MQ=255) tATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTtcc < 1:2406726/70‑1 (MQ=255) tCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAAtgatga < 1:1538276/55‑1 (MQ=255) tCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAAtgatga < 1:5384567/55‑1 (MQ=255) tCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTtcctcc > 1:2529112/1‑71 (MQ=255) cGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTcctcct < 1:5342248/70‑1 (MQ=255) cGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTg > 1:5491644/1‑71 (MQ=255) cGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTg > 1:3330444/1‑71 (MQ=255) tCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTg < 1:3229031/69‑1 (MQ=255) tCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTg < 1:5372862/69‑1 (MQ=255) ttGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGatat > 1:4034113/1‑52 (MQ=255) ttGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGAc > 1:1955615/1‑71 (MQ=255) ttGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCG‑AATGATGATATGAGTTGTTCCTCCTGCGACg > 1:1619006/1‑71 (MQ=255) ttGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCG‑AATGATGATATGAGTTGTTCCTCCTGCGACg > 1:1625714/1‑71 (MQ=255) gTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGAc < 1:1230706/69‑1 (MQ=255) ttCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAAtgatga < 1:4301107/46‑1 (MQ=255) ttCTGCCCCAGGATGAGACTATACATCGTCGAGATCGCAAtgatga > 1:4292406/1‑46 (MQ=255) cTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAAtgatga < 1:1971366/44‑1 (MQ=255) cTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGctc > 1:4362226/1‑71 (MQ=255) cTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGctc > 1:5671987/1‑71 (MQ=255) cTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGct > 1:3810355/1‑70 (MQ=255) ccGCAGGATGAGACTATACATCGTCGAGATCGCAatga > 1:4033043/1‑38 (MQ=255) ccGCAGGATGAGACTATACATCGTCGAGATCGCAatga > 1:2298830/1‑38 (MQ=255) ccGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGctct < 1:5208430/69‑1 (MQ=255) cGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCg > 1:3029933/1‑60 (MQ=255) cAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCa < 1:4786608/71‑1 (MQ=255) aGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGAc < 1:3721742/59‑1 (MQ=255) gATGAGACTATACATCGTCGAGATCGCAATGATGATATGAgtt < 1:3708620/43‑1 (MQ=255) gATGAGACTATACATCGTCGAGATCGCAATGATGATATGAgtt < 1:4446608/43‑1 (MQ=255) gATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCaaaa < 1:5505732/71‑1 (MQ=255) gATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCaaaa < 1:3290580/71‑1 (MQ=255) gATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCaaaa < 1:258067/71‑1 (MQ=255) aTGAGACTATACATCGTCGAGATCGCAATGATGATATGAgtt < 1:4218532/42‑1 (MQ=255) tGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTaa > 1:5210211/1‑63 (MQ=255) tGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCaaaa > 1:2546351/1‑69 (MQ=255) tGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCa > 1:4208551/1‑66 (MQ=255) tGAGACGATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTaa > 1:1459/1‑63 (MQ=255) agaCTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAag < 1:3733606/70‑1 (MQ=255) aCTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAagag > 1:242859/1‑70 (MQ=255) tataCATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCaaaaaa > 1:171021/1‑65 (MQ=255) taCATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCTACGGCTCTAATCAAAAAAGAGCAgg < 1:3670887/70‑1 (MQ=255) taCATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAAt < 1:5045480/56‑1 (MQ=255) taCATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAAGAGCAgg < 1:2745143/70‑1 (MQ=255) taCATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAAGAGCAg > 1:4592541/1‑69 (MQ=255) cATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGctc > 1:63521/1‑50 (MQ=255) cATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCa > 1:4450503/1‑56 (MQ=255) cATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCa > 1:3092649/1‑56 (MQ=255) cATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAAGAGCAGGAGt > 1:4219500/1‑71 (MQ=255) aTCGTCGAGATCGCAATGATGATATGAGTTGTtcct > 1:4080725/1‑36 (MQ=255) aTCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAAGAGCAg < 1:1990394/66‑1 (MQ=255) tcgtcgAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAAGAGCAGGAGTGc > 1:224432/1‑71 (MQ=255) tcgtcgAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAAGAGCAGGAGTGc > 1:3781592/1‑71 (MQ=255) tcgtcgAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAAGAGCAGGAGTGc > 1:1351916/1‑71 (MQ=255) gaTCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTaa > 1:4148712/1‑44 (MQ=255) gaTCGCAATGATGATATGAGTTGTTCCTCCTGCGAAGGCTCTaa > 1:1148926/1‑44 (MQ=255) aTCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCaaaa < 1:2593939/49‑1 (MQ=255) aTCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAag < 1:124382/52‑1 (MQ=255) aTCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAag < 1:4736522/52‑1 (MQ=255) aTCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAag < 1:4729998/52‑1 (MQ=255) aTCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAAGAGCAgg < 1:84878/58‑1 (MQ=255) aTCGCAATCATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCaaaa < 1:4850145/49‑1 (MQ=255) cGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAAGAGCAGGAg > 1:5447684/1‑58 (MQ=255) cGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAAGAGCAGGAGTGCTTTTCCATc > 1:2793358/1‑70 (MQ=255) cGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAAGAGCAGGAGTGCTTTTCCATc > 1:1901843/1‑70 (MQ=255) aaTGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAAGAGCTGGAGTGCtt > 1:1967423/1‑60 (MQ=255) aaTGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAAGAGCAGGAGTGCtt > 1:4438096/1‑60 (MQ=255) atgatgATATGAGTTGTTCCTCCTGCGACGGCTCTAATCaaaa < 1:3310542/43‑1 (MQ=255) atgatgATATGAGTTGTTCCTCCTGCGACGGCTCTAATCa < 1:5639258/40‑1 (MQ=255) tgatgaTATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAAGAGCAg < 1:1920484/50‑1 (MQ=255) tgatgaTATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAAGAGCAGGAGTGCTTTTCCATCTATTGc < 1:1911131/71‑1 (MQ=255) tgatgaTATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAAGAGCAGGAGTGCTTTTCCATCTATTGc > 1:716569/1‑71 (MQ=255) | TGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAAGAGCAGGAGTGCTTTTCCATCTATTGC > Exported/2571355‑2571494 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |