| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,572,751 | T→A | 30.3% | intergenic (+1519/‑260) | yibH → / → ysaB | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,572,751 | 0 | T | A | 30.3% | 161.4 / 93.2 | 112 | intergenic (+1519/‑260) | yibH/ysaB | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (41/37); new base A (21/13); total (62/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.13e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01 | |||||||||||
Only 100 of 136 total aligned reads displayed.
CAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTC > Exported/2572681‑2572820 | cAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCctgct > 1:3994771/1‑71 (MQ=255) cGGATACTGATAAAAATTCCATCCCCCCTTCAGCCCAATCGGATCCTGAGTGATATACCGCCCCTGCAGCg < 1:3995756/71‑1 (MQ=255) cGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCg > 1:5666993/1‑71 (MQ=255) ggATACTGATAAAAATTCCATCCCCCCTTCAGCCCAATCGGATCCTGAGTGATATACCGCCCCTGCAGCgg > 1:5171066/1‑71 (MQ=255) aTACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGt > 1:2729850/1‑70 (MQ=255) tACTGATAAAAATTCCATCCCCCTTTCAGCCCAATCGGATCCTGAGTGATATATCGCCCCTGCAGCgggtc < 1:2850444/71‑4 (MQ=255) tGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGtt < 1:4968894/67‑1 (MQ=255) gATAAAAATTCCATCCCCCCTTCAGTCCAATCGGATCCTGAGTGATATACCGCCCCAGCAGCgg > 1:4979797/1‑64 (MQ=255) gATAAAAATTCCATCCCCCCTTCAGTCCAATCGGATCCTGAGTGATATACCGCCCCAGCAGCgg > 1:1934447/1‑64 (MQ=255) aTAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATg > 1:943676/1‑69 (MQ=255) aTAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGtt < 1:1445588/71‑1 (MQ=255) tAAAAATTCCATCCCCCCTTCAGTCCAATCGGATCCTGAGTGATATACCGCCCCagcagc < 1:1807372/60‑1 (MQ=255) tAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCtgctgc < 1:2339800/60‑1 (MQ=255) aaaaaTTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTAc > 1:1167399/1‑71 (MQ=255) aaaaTTCCACCTACCTTCCAGCCCAATCGGAGCATGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTAcc > 1:4425874/1‑71 (MQ=255) ttCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGat > 1:3171790/1‑70 (MQ=255) ccACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGataa > 1:4211728/1‑70 (MQ=255) ccACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGataa < 1:5065269/70‑1 (MQ=255) cACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGataat < 1:2640986/70‑1 (MQ=255) acctacctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCtgctgc < 1:309814/50‑1 (MQ=255) acctacctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCtgctgc < 1:514564/50‑1 (MQ=255) cctacctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGt < 1:2116581/71‑1 (MQ=255) ctacctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCg > 1:2150441/1‑49 (MQ=255) ctacctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGt > 1:2631853/1‑70 (MQ=255) acctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTta > 1:2439551/1‑70 (MQ=255) cctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTTCGGTTAATGTTACCGATAATAGTta < 1:3063471/69‑1 (MQ=255) cctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGa < 1:3796863/60‑1 (MQ=255) cctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTta < 1:5310379/69‑1 (MQ=255) cctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTta < 1:4763221/69‑1 (MQ=255) ctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAg > 1:1943673/1‑71 (MQ=255) ctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAg < 1:5513053/71‑1 (MQ=255) ttCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTa > 1:815782/1‑54 (MQ=255) ccAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTTCTGCGGTTAATGTTACCGATAATAGTTATAGt < 1:5113808/69‑1 (MQ=255) cAGCCCAATCGGATCCTGAGTGATATACCGCCCCTGCAGCgg > 1:701797/1‑42 (MQ=255) cAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTa > 1:2144679/1‑69 (MQ=255) cAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTa > 1:4094487/1‑69 (MQ=255) cAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTa > 1:1811317/1‑69 (MQ=255) cAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTAc < 1:2124118/70‑1 (MQ=255) cccAATCGGGTCCGGGGTGATATACCGCCCCTGCAACGGATCGTAGTGCCGGTGACGGTTATAGTACAGcc > 1:64164/1‑71 (MQ=255) cccaTCGGGTCCTGAGTAATATACCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGccc > 1:1181916/4‑71 (MQ=255) cAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGata > 1:3425088/1‑52 (MQ=255) cAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCt < 1:28268/71‑1 (MQ=255) caTCGGGTCCTGAGTAATATACCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGCCCTg < 1:2543541/70‑1 (MQ=255) caTCGGGTCCTGAGTAATATACCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGCCCTg < 1:3753246/70‑1 (MQ=255) aaTCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTaa < 1:4299080/40‑1 (MQ=255) aTCGGGTCCTGAGTAATATACCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGCCCTGa > 1:3363095/1‑71 (MQ=255) aTCGGGTCCTGAGTAATATACCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGCACTGa > 1:3547067/1‑71 (MQ=255) aTCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGtt > 1:2463427/1‑43 (MQ=255) ggAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGAtt > 1:5074099/1‑70 (MQ=255) ggAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtc < 1:3677966/71‑1 (MQ=255) tccTGAGTAATATACCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGCCCTGATtcctc < 1:2827269/70‑1 (MQ=255) ccTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGt < 1:5209863/54‑1 (MQ=255) ccTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatca > 1:1458927/1‑71 (MQ=255) cTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcat < 1:1215386/71‑1 (MQ=255) tGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGt < 1:1550200/52‑1 (MQ=255) tGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTAc > 1:3606836/1‑54 (MQ=255) tGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcat > 1:4255053/1‑70 (MQ=255) tGAGTAATATACCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGCCCTGATtcctcat > 1:4848809/1‑70 (MQ=255) ggtgaTATACCGCCCCTGCAACGGATCGTAGTGCCGGTGACGGTTATAGTACAGCCCCGACTcctcatcat < 1:3500579/70‑1 (MQ=255) aGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcatca > 1:4364238/1‑70 (MQ=255) aGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcatca < 1:4024081/70‑1 (MQ=255) gtgtTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGAtt > 1:1850727/1‑61 (MQ=255) gtgtTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGAtt > 1:3075723/1‑61 (MQ=255) taatataCCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGCCCTGATtcctcat < 1:1756528/63‑1 (MQ=255) gtTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcat < 1:3750460/65‑1 (MQ=255) gtTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcat < 1:3995348/65‑1 (MQ=255) ttATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCt < 1:4608315/54‑1 (MQ=255) ttATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCt < 1:459614/54‑1 (MQ=255) ttATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTcatcatcat < 1:1729571/67‑1 (MQ=255) atataCCGCCCCTGCAACGGATCGTAGTGCCGGTGACGGTTATAGTACAGcccc > 1:1408611/2‑53 (MQ=255) atataCCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGCCCTGATtcctc > 1:2377207/2‑62 (MQ=255) atataCCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGCCCTGATTCCTCATCAtgctg > 1:5647503/2‑70 (MQ=255) ataCCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTtata > 1:1599657/1‑42 (MQ=255) ataCCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTtata > 1:3483156/1‑42 (MQ=255) ataCCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTtat > 1:4843679/1‑41 (MQ=255) taCCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGcc > 1:3381715/1‑49 (MQ=255) taCCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGcc > 1:3598182/1‑49 (MQ=255) taCCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGCCCTGATTCCTCATCATGctgctg > 1:1084867/1‑71 (MQ=255) aCCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcat < 1:3216827/60‑1 (MQ=255) ccGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTActgct < 1:3679169/71‑1 (MQ=255) ccccTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCg > 1:1675088/1‑71 (MQ=255) ccTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGa > 1:4879658/1‑46 (MQ=255) ccTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGa > 1:707945/1‑46 (MQ=255) ccTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCAtact < 1:4925133/60‑1 (MQ=255) ccTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATactactact < 1:2410494/66‑1 (MQ=255) ccTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGc < 1:527883/71‑1 (MQ=255) ctgctgCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcatca < 1:878015/55‑1 (MQ=255) ctgctgCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcatca < 1:1131489/55‑1 (MQ=255) ctgctgCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcatca < 1:3793395/55‑1 (MQ=255) ctgctgCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcatca < 1:2079703/55‑1 (MQ=255) tgctgcGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcatca < 1:303116/54‑1 (MQ=255) tgctgcGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcatca < 1:105533/54‑1 (MQ=255) tgctgcGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcatca < 1:4503456/54‑1 (MQ=255) tgctgcGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcatca < 1:4509804/54‑1 (MQ=255) tgctgcGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAg > 1:3132992/1‑71 (MQ=255) gctgcGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcat < 1:1960161/51‑1 (MQ=255) gctgcGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcat < 1:946158/51‑1 (MQ=255) gctgcGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGc > 1:3048195/1‑68 (MQ=255) ctgcGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGt < 1:4689544/70‑1 (MQ=255) ctgcGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTc > 1:67307/1‑71 (MQ=255) | CAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTC > Exported/2572681‑2572820 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |