Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | Exported | 2,584,283 | T→C | 8.2% | Q998Q (CAA→CAG) | mdtF ← | multidrug transporter, RpoS‑dependent |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | Exported | 2,584,283 | 0 | T | C | 8.2% | 203.7 / 6.1 | 73 | Q998Q (CAA→CAG) | mdtF | multidrug transporter, RpoS‑dependent |
Reads supporting (aligned to +/- strand): ref base T (41/26); new base C (2/4); total (43/30) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.21e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AACGAAGTAAATTGCCAGCACTGTTGCGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAG > Exported/2584217‑2584349 | aaCGAAGTAAATTGCCAGCACTGTTGCGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTgc > 1:5464394/1‑70 (MQ=255) aCGAAGTAAATTGCCAGCACTGTTGCGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTgcgc < 1:4191195/71‑1 (MQ=255) aCGAAGTAAATTGCCAGCACTGTTGCGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTgcgc < 1:313613/71‑1 (MQ=255) aaGTAAATTGCCAGCACTGTTGCGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTgcgcgc > 1:1203647/1‑70 (MQ=255) aaaTTGCCAGCACTGTTGCGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTg > 1:2673245/1‑61 (MQ=255) aaaTTGCCAGCACTGTTGCGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGaa > 1:2218682/1‑71 (MQ=255) aaTTGCCAGCACTGTTGCGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTgcg > 1:3031471/1‑62 (MQ=255) aaTTGCCAGCACTGTTGCGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAAc > 1:2807942/1‑71 (MQ=255) aTTGCCAGCACTGTTGCGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAAcc < 1:956389/71‑1 (MQ=255) cAGCACTGTTGCGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCg > 1:4007632/1‑67 (MQ=255) gCACTGTTGCGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGtttt > 1:4456243/1‑51 (MQ=255) gCACTGTTGCGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCTCCAGAACCGGCa > 1:1079461/1‑68 (MQ=255) gCACTGTTGCGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAAc > 1:1213764/1‑63 (MQ=255) gCACTGTTGCGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCa > 1:968101/1‑68 (MQ=255) cACTGTTGCGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCAt < 1:2206919/71‑1 (MQ=255) cACTGTTGCGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCAt < 1:2392213/71‑1 (MQ=255) aCTGTTGCGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCAt < 1:8923/70‑1 (MQ=255) tGTTGCGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGa > 1:3745073/1‑70 (MQ=255) gTTGCGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCac > 1:4058827/1‑54 (MQ=255) gTTGCGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGAc > 1:2874533/1‑70 (MQ=255) gTTGCGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACt > 1:3526394/1‑71 (MQ=255) ttGCGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACt > 1:4011988/1‑70 (MQ=255) ttGCGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTg < 1:5018544/71‑1 (MQ=255) tGCGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGtttt > 1:4769934/1‑44 (MQ=255) gCGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGa < 1:3806177/70‑1 (MQ=255) gCGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGa < 1:1727403/70‑1 (MQ=255) gCGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGa < 1:1577447/70‑1 (MQ=255) cGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGAt < 1:1188193/70‑1 (MQ=255) ggCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATaa < 1:3434867/71‑1 (MQ=255) gCAAACATCCCGCCCATCACGCCGGTACCTACCGCGttt < 1:869946/39‑1 (MQ=255) gCAAACATCCCACCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAAc < 1:1517813/71‑1 (MQ=255) cAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAAc > 1:2414109/1‑70 (MQ=255) cAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAAc < 1:859642/70‑1 (MQ=255) cAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAAc > 1:3157592/1‑70 (MQ=255) aaaCATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAAc > 1:3675681/1‑69 (MQ=255) aaaCATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAAc > 1:2202186/1‑69 (MQ=255) aaCATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAg > 1:1900021/1‑71 (MQ=255) aCATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGc < 1:2005434/71‑1 (MQ=255) cATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGc < 1:3667268/70‑1 (MQ=255) cATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCg > 1:340559/1‑71 (MQ=255) aTCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAAc > 1:636662/1‑46 (MQ=255) aTCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGa > 1:2902715/1‑57 (MQ=255) cccATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCAc > 1:3668171/1‑71 (MQ=255) cccATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCAc > 1:2568325/1‑71 (MQ=255) ccATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACg < 1:765931/71‑1 (MQ=255) cATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGc > 1:5622025/1‑71 (MQ=255) tCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGAt < 1:238504/52‑1 (MQ=255) aCGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGcc > 1:5471326/1‑68 (MQ=255) ccGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGaa > 1:531115/1‑70 (MQ=255) ccGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGaa > 1:1165698/1‑70 (MQ=255) cgatacccacaGCGTTCTGCGCGCCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAAt > 1:1730401/4‑70 (MQ=9) cgatacccacaGCGTTCTGCGCGCCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATa < 1:2237258/68‑1 (MQ=9) cGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGAc > 1:5354845/1‑42 (MQ=255) cGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAAt > 1:1950990/1‑70 (MQ=255) cGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATa < 1:5205726/71‑1 (MQ=255) cGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATa < 1:2445552/71‑1 (MQ=255) gTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATaaa < 1:1516042/71‑1 (MQ=255) gTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATa > 1:2285179/1‑69 (MQ=255) atacccacaGCGTTCTGCGCGCCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATaaa < 1:852092/70‑1 (MQ=9) atacccacaGCGTTCTGCGCGCCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATaaa < 1:4123503/70‑1 (MQ=9) tacccacaGCGTTCTGCGCGCCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAg < 1:4715207/71‑1 (MQ=9) cctaccGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAg < 1:5339496/47‑1 (MQ=255) cctaccGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGc > 1:3371844/1‑71 (MQ=255) accGCGTTTTGCGCGCCAGAACCGGCACCATGACTGa > 1:4548272/1‑37 (MQ=255) accGCGTTTTGAGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCAc > 1:384676/1‑53 (MQ=255) ccGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCa > 1:2544561/1‑69 (MQ=255) ccGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCa > 1:1625428/1‑69 (MQ=255) cgcgTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCagag < 1:1895414/71‑1 (MQ=255) cgcgTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCagag < 1:4008447/71‑1 (MQ=255) cgcgTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGCCAGCACGCCGAGAATAAAGGCCagag < 1:768882/71‑1 (MQ=255) agcgTTCTGCGCGCCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGcga > 1:4694479/2‑66 (MQ=9) gcgTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATaa > 1:1059270/1‑61 (MQ=255) gcgTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATaa > 1:5171755/1‑61 (MQ=255) gcgTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATaa > 1:3908394/1‑61 (MQ=255) cgTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCagagag > 1:5291444/1‑71 (MQ=255) gTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCaga < 1:4306235/67‑1 (MQ=255) ttttGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGcc < 1:4764709/50‑1 (MQ=255) ttttGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGcc < 1:4760626/50‑1 (MQ=255) ttttGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGcc < 1:4521918/50‑1 (MQ=255) ttttGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGc < 1:4992602/62‑1 (MQ=255) ttttGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCaga < 1:5564802/66‑1 (MQ=255) ttttGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCaga < 1:562964/66‑1 (MQ=255) ttttGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCa < 1:4791385/64‑1 (MQ=255) ttttGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCa < 1:3214057/64‑1 (MQ=255) | AACGAAGTAAATTGCCAGCACTGTTGCGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAG > Exported/2584217‑2584349 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |