BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	2,595,216	G→T	10.0%	T27K (ACA→AAA) ‡	arsC ←	arsenate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	2,595,216	0	G	T	10.0%	309.2 / 11.0	98	T27K (ACA→AAA) ‡	arsC	arsenate reductase
Reads supporting (aligned to +/- strand): ref base G (41/47); new base T (3/7); total (44/54)
Fisher's exact test for biased strand distribution p-value = 5.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 108 total aligned reads displayed.

TCGGCAATGAGTTTGACCAGTTCATCGCGCGTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGT  >  Exported/2595147‑2595283

tCGGCAATGAGTTTGACCAGTTCATCGCGCGTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCtgt                                                                    <  1:2903383/71‑1 (MQ=255)

tCGGCAATGAGTTTGACCAGTTCATCGCGCGTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCtgt                                                                    <  1:4015992/71‑1 (MQ=255)

      aTGAGTTTGACCAGTTCATCGCGCGTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCt                                                              <  1:1599215/71‑1 (MQ=255)

      aTGAGTTTGACCAGTTCATCGCGCGTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTGCTGTGCCGCt                                                              <  1:5434576/71‑1 (MQ=255)

        gAGTTTGACCAGTTCATCGCGCGTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTg                                                             >  1:3610266/1‑70 (MQ=255)

        gAGTTTGACCAGTTCATCGCGCGTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTg                                                             >  1:3059578/1‑70 (MQ=255)

        gAGTTTGACCAGTTCATCGCGCGTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTg                                                             >  1:4004157/1‑70 (MQ=255)

        gAGTTTGACCAGTTCATCGCGCGTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGt                                                            >  1:1113835/1‑71 (MQ=255)

        gAGTTTGACCAGTTCATCGCGCGTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGt                                                            >  1:2506547/1‑71 (MQ=255)

         aGTTTGACCAGTTCATCGCGCGTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGtt                                                           >  1:3375841/1‑71 (MQ=255)

             tGACCAGTTCATCGCGCGTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCtgtg                                                                   >  1:1765867/1‑59 (MQ=255)

             tGACCAGTTCATCGCGCGTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCg                                                        >  1:3540696/1‑70 (MQ=255)

             tGACCAGTTCATCGCGCGTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCg                                                        >  1:420386/1‑70 (MQ=255)

                    ttCATCGCGCGTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGc                                                         <  1:3169537/62‑1 (MQ=255)

                    ttCATCGCGCGTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCAtct                                                <  1:4188503/71‑1 (MQ=255)

                    ttCATCGCGCGTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCAtct                                                <  1:1235344/71‑1 (MQ=255)

                      cATCGCGCGTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCgg                                                       >  1:5314449/1‑62 (MQ=255)

                      cATCGCGCGTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCgg                                                       >  1:3305240/1‑62 (MQ=255)

                      cATCGCGCGTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCAtctc                                               >  1:3720909/1‑70 (MQ=255)

                      cATCGCGCGTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCAtctc                                               >  1:156286/1‑70 (MQ=255)

                       aTCGCGCGTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCgg                                                       >  1:398630/1‑61 (MQ=255)

                       aTCGCGCGTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTcc                                              <  1:677122/70‑1 (MQ=255)

                         cgcgcgTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGc                                                         <  1:3772907/57‑1 (MQ=255)

                          gcgcgTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCg                                          <  1:1662411/71‑1 (MQ=255)

                            gcgTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTa                                        <  1:4507579/71‑1 (MQ=255)

                             cgTTGGCGGAGTTTCCAGATAATGGATAATAGTCTGTTCTGTGCCGCTGTTGCGGatc                                                    <  1:5663473/58‑1 (MQ=255)

                             cgTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGatc                                                    <  1:1422093/58‑1 (MQ=255)

                             cgTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTAt                                       <  1:1505373/71‑1 (MQ=255)

                               ttGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCtgt                                                                    <  1:727457/40‑1 (MQ=255)

                               ttGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCgg                                                       <  1:3189027/53‑1 (MQ=255)

                                tGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCtg                                                                     >  1:5314860/1‑38 (MQ=255)

                                tGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCtg                                                                     >  1:1582131/1‑38 (MQ=255)

                                tGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCtg                                                                     >  1:4508467/1‑38 (MQ=255)

                                 ggCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTAc                                    <  1:1590286/70‑1 (MQ=255)

                                  gCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCg                                                        <  1:1617702/49‑1 (MQ=255)

                                  gCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTAcgc                                  <  1:2444658/71‑1 (MQ=255)

                                  gCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTAcg                                   <  1:772138/70‑1 (MQ=255)

                                    ggAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGa                                <  1:26413/71‑1 (MQ=255)

                                    ggAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGa                                <  1:3161115/71‑1 (MQ=255)

                                      aGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGatcat                                                  >  1:2306733/1‑51 (MQ=255)

                                      aGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACg                              >  1:214205/1‑71 (MQ=255)

                                       gTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGt                             <  1:3291164/71‑1 (MQ=255)

                                        tttCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTg                                                             <  1:5458828/38‑1 (MQ=255)

                                        tttCCAGATAATAGATAATGGTCGGTTCTGTACCGCTGTTGCGGATCATCTCCAGCGTATTACGTGACGt                             <  1:1088262/70‑1 (MQ=255)

                                         ttCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCg                                          <  1:4389756/56‑1 (MQ=255)

                                         ttCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGAc                               <  1:32204/67‑1 (MQ=255)

                                          tcgaGATAATTAATTATTGTCGGTTCGTTGCCGTTGTTATGAAGCATCTCCAGCGTGTTGCGTGAGGTGc                           >  1:3430818/4‑70 (MQ=255)

                                          tCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGtt                                                           >  1:3198932/1‑38 (MQ=255)

                                          tCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGc                                           >  1:5279192/1‑54 (MQ=255)

                                          tCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGcc                          >  1:532932/1‑71 (MQ=255)

                                           ccAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCg                         >  1:2515271/1‑71 (MQ=255)

                                            gaGATAATTAATTATTGTCGGTTCGTTGCCGTTGTTATGAAGCATCTCCAGCGTGTTGCGTGAGGTGccac                        <  1:5354453/70‑3 (MQ=255)

                                            gaGATAATTAATTATTGTCGGTTCGTTGCCGTTGTTATGAAGCATCTCCAGCGTGTTGCGTGAGGTGcca                         <  1:1903951/69‑2 (MQ=255)

                                            gaGATAATTAATTATTGTCGGTTCGTTGCCGTTGTTATGAAGCATCTCCAGCGTGTTGCGTGAGGTGcca                         <  1:4622529/69‑2 (MQ=255)

                                            gaGATAATTAATTATTGTCGGTTCGTTGCCGTTGTTATGAAGCATCTCCAGCGTGTTGCGTGAGGTGcca                         <  1:3918693/69‑2 (MQ=255)

                                            cAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTAcgc                                  <  1:2921762/61‑1 (MQ=255)

                                            cAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTAcgc                                  <  1:2770303/61‑1 (MQ=255)

                                               aTAATTAATTATTGTCGGTTCGTTGCCGTTGTTATGAAGCATCTCCAGCg                                          <  1:4125509/50‑1 (MQ=255)

                                               aTAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCg                                          <  1:2008825/50‑1 (MQ=255)

                                               aTAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCg                                          <  1:1369154/50‑1 (MQ=255)

                                                  aTGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCa                       >  1:3825895/1‑66 (MQ=255)

                                                  aTGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCa                       >  1:4934723/1‑66 (MQ=255)

                                                     gATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAg                                            <  1:1751469/42‑1 (MQ=255)

                                                     gATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAg                                            <  1:559705/42‑1 (MQ=255)

                                                     gATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAg                                            <  1:3649309/42‑1 (MQ=255)

                                                     gATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAg                                            <  1:589468/42‑1 (MQ=255)

                                                     gATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAg                                            <  1:3691019/42‑1 (MQ=255)

                                                     gATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAg                                            <  1:2043411/42‑1 (MQ=255)

                                                     gATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTa                                        >  1:631560/1‑46 (MQ=255)

                                                     gATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTa                                        >  1:1638362/1‑46 (MQ=255)

                                                     gATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGt                             >  1:446952/1‑57 (MQ=255)

                                                     gATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGt               >  1:2619882/1‑71 (MQ=255)

                                                      ataataGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGtt              >  1:2615485/1‑71 (MQ=255)

                                                         attgTCGGTTCGTTGCCGTTGTTATGAAGCATCTCCAGCGTGTTGCGTGAGGTGCCACAGGCCGGGTtgt            >  1:765989/4‑70 (MQ=255)

                                                         attgTCGGTTCGTTGCCGTTGTTATGAAGCATCTCCAGCGTGTTGCGTGAGGTGCCACAGGCCGGGTtgt            <  1:2990478/67‑1 (MQ=255)

                                                         ataGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTtgt            <  1:1234973/70‑1 (MQ=255)

                                                          taGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTa                                     >  1:2270696/1‑44 (MQ=255)

                                                          taGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGcc                          <  1:5624812/55‑1 (MQ=255)

                                                          taGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGcc                          <  1:5451187/55‑1 (MQ=255)

                                                          taGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGcc                          <  1:3382434/55‑1 (MQ=255)

                                                            gTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTAtt                                      >  1:5068052/1‑41 (MQ=255)

                                                            gTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCCTAtt                                      >  1:4003727/1‑41 (MQ=255)

                                                            gTCGGTTCGTTGCCGTTGTTATGAAGCATCTCCAGCGTGTTGCGTGAGGTGCCACAGGCCGGGTTGTGa          >  1:1025140/1‑69 (MQ=255)

                                                             tCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATaa       <  1:930599/71‑1 (MQ=255)

                                                             tCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATaa       <  1:1132343/71‑1 (MQ=255)

                                                             tCGGTTCGTTGCCGTTGTTATGAAGCATCTCCAGCGTGTTGCGTGAGGTGCCACAGGCCGGGTTGTGATa        <  1:3370188/70‑1 (MQ=255)

                                                              cGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTa                                        >  1:4416215/1‑37 (MQ=255)

                                                              cGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATaaa      >  1:5471374/1‑71 (MQ=255)

                                                               ggTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAAt     >  1:5296498/1‑71 (MQ=255)

                                                                gTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCg                         <  1:5081422/50‑1 (MQ=255)

                                                                gTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCg                         <  1:3248690/50‑1 (MQ=255)

                                                                gTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCg                         <  1:410810/50‑1 (MQ=255)

                                                                 ttCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATgg   >  1:5562081/1‑71 (MQ=255)

                                                                 ttCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATgg   <  1:5046617/71‑1 (MQ=255)

                                                                   cTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGt  <  1:1929783/70‑1 (MQ=255)

                                                                   cTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGt  >  1:2132299/1‑70 (MQ=255)

                                                                   cTGTGCCGCTGTTGCAGATCATCTCCAGCGTATTACGCGACGTGCCGCa                       >  1:3609444/1‑49 (MQ=255)

                                                                    tgtgCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGa                                <  1:4425046/39‑1 (MQ=255)

                                                                    tgtgCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCg                  <  1:5660910/53‑1 (MQ=255)

                                                                    tgtgCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCg                  <  1:2059901/53‑1 (MQ=255)

TCGGCAATGAGTTTGACCAGTTCATCGCGCGTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGT  >  Exported/2595147‑2595283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`