BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	2,595,227	G→A	11.6%	R23R (CGC→CGT) ‡	arsC ←	arsenate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	2,595,227	0	G	A	11.6%	297.3 / 22.2	112	R23R (CGC→CGT) ‡	arsC	arsenate reductase
Reads supporting (aligned to +/- strand): ref base G (39/60); new base A (3/10); total (42/70)
Fisher's exact test for biased strand distribution p-value = 3.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 114 total aligned reads displayed.

TGACCAGTTCATCGCGCGTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATA  >  Exported/2595160‑2595296

tGACCAGTTCATCGCGCGTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCg                                                                     >  1:420386/1‑70 (MQ=255)

tGACCAGTTCATCGCGCGTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCg                                                                     >  1:3540696/1‑70 (MQ=255)

      gTTCATCGCGCGTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGatcat                                                               >  1:5034946/1‑70 (MQ=255)

       ttCATCGCGCGTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGc                                                                      <  1:3169537/62‑1 (MQ=255)

       ttCATCGCGCGTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCAtct                                                             <  1:4188503/71‑1 (MQ=255)

       ttCATCGCGCGTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCAtct                                                             <  1:1235344/71‑1 (MQ=255)

         cATCGCGCGTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCgg                                                                    >  1:5314449/1‑62 (MQ=255)

         cATCGCGCGTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCgg                                                                    >  1:3305240/1‑62 (MQ=255)

         cATCGCGCGTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCAtctc                                                            >  1:3720909/1‑70 (MQ=255)

         cATCGCGCGTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCAtctc                                                            >  1:156286/1‑70 (MQ=255)

          aTCGCGCGTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCgg                                                                    >  1:398630/1‑61 (MQ=255)

          aTCGCGCGTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTcc                                                           <  1:677122/70‑1 (MQ=255)

            cgcgcgTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGc                                                                      <  1:3772907/57‑1 (MQ=255)

             gcgcgTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCg                                                       <  1:1662411/71‑1 (MQ=255)

               gcgTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTa                                                     <  1:4507579/71‑1 (MQ=255)

                cgTTGGCGGAGTTTCCAGATAATGGATAATAGTCTGTTCTGTGCCGCTGTTGCGGatc                                                                 <  1:5663473/58‑1 (MQ=255)

                cgTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGatc                                                                 <  1:1422093/58‑1 (MQ=255)

                  ttGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCgg                                                                    <  1:3189027/53‑1 (MQ=255)

                    ggCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTAc                                                 <  1:1590286/70‑1 (MQ=255)

                     gCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCg                                                                     <  1:1617702/49‑1 (MQ=255)

                     gCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTAcgc                                               <  1:2444658/71‑1 (MQ=255)

                     gCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTAcg                                                <  1:772138/70‑1 (MQ=255)

                       ggAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGa                                             <  1:3161115/71‑1 (MQ=255)

                       ggAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGa                                             <  1:26413/71‑1 (MQ=255)

                         aGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGatcat                                                               >  1:2306733/1‑51 (MQ=255)

                           tttCCAGATAATAGATAATGGTCGGTTCTGTACCGCTGTTGCGGATCATCTCCAGCGTATTACGTGACGt                                          <  1:1088262/70‑1 (MQ=255)

                            ttCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCg                                                       <  1:4389756/56‑1 (MQ=255)

                            ttCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGAc                                            <  1:32204/67‑1 (MQ=255)

                             tcgaGATAATTAATTATTGTCGGTTCGTTGCCGTTGTTATGAAGCATCTCCAGCGTGTTGCGTGAGGTGc                                        >  1:3430818/4‑70 (MQ=255)

                             tCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGc                                                        >  1:3515282/1‑54 (MQ=255)

                             tCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGcc                                       >  1:532932/1‑71 (MQ=255)

                               gaGATAATTAATTATTGTCGGTTCGTTGCCGTTGTTATGAAGCATCTCCAGCGTGTTGCGTGAGGTGccac                                     <  1:5354453/70‑3 (MQ=255)

                               gaGATAATTAATTATTGTCGGTTCGTTGCCGTTGTTATGAAGCATCTCCAGCGTGTTGCGTGAGGTGcca                                      <  1:1903951/69‑2 (MQ=255)

                               gaGATAATTAATTATTGTCGGTTCGTTGCCGTTGTTATGAAGCATCTCCAGCGTGTTGCGTGAGGTGcca                                      <  1:4622529/69‑2 (MQ=255)

                               gaGATAATTAATTATTGTCGGTTCGTTGCCGTTGTTATGAAGCATCTCCAGCGTGTTGCGTGAGGTGcca                                      <  1:3918693/69‑2 (MQ=255)

                               cAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTAcgc                                               <  1:2921762/61‑1 (MQ=255)

                               cAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTAcgc                                               <  1:2770303/61‑1 (MQ=255)

                                  aTAATTAATTATTGTCGGTTCGTTGCCGTTGTTATGAAGCATCTCCAGCg                                                       <  1:4125509/50‑1 (MQ=255)

                                  aTAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCg                                                       <  1:2008825/50‑1 (MQ=255)

                                  aTAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCg                                                       <  1:1369154/50‑1 (MQ=255)

                                     aTGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCa                                    >  1:3825895/1‑66 (MQ=255)

                                        gATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAg                                                         <  1:3691019/42‑1 (MQ=255)

                                        gATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAg                                                         <  1:3649309/42‑1 (MQ=255)

                                        gATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAg                                                         <  1:1751469/42‑1 (MQ=255)

                                        gATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAg                                                         <  1:559705/42‑1 (MQ=255)

                                        gATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAg                                                         <  1:2043411/42‑1 (MQ=255)

                                        gATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTa                                                     >  1:631560/1‑46 (MQ=255)

                                        gATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTa                                                     >  1:1638362/1‑46 (MQ=255)

                                        gATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGt                                          >  1:446952/1‑57 (MQ=255)

                                        gATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGt                            >  1:2619882/1‑71 (MQ=255)

                                         ataataGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGtt                           >  1:2615485/1‑71 (MQ=255)

                                          taataGTCGGTTCTGTGCC‑CTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTtgt                         <  1:552968/71‑1 (MQ=255)

                                            attgTCGGTTCGTTGCCGTTGTTATGAAGCATCTCCAGCGTGTTGCGTGAGGTGCCACAGGCCGGGTtgt                         >  1:765989/4‑70 (MQ=255)

                                            attgTCGGTTCGTTGCCGTTGTTATGAAGCATCTCCAGCGTGTTGCGTGAGGTGCCACAGGCCGGGTtgt                         <  1:2990478/67‑1 (MQ=255)

                                            ataGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTtgt                         <  1:1234973/70‑1 (MQ=255)

                                             taGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTa                                                  >  1:2270696/1‑44 (MQ=255)

                                             taGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTa                                                  >  1:2146850/1‑44 (MQ=255)

                                             taGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGcc                                       <  1:3382434/55‑1 (MQ=255)

                                             taGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGcc                                       <  1:5451187/55‑1 (MQ=255)

                                             taGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGcc                                       <  1:5624812/55‑1 (MQ=255)

                                               gTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTAtt                                                   >  1:5068052/1‑41 (MQ=255)

                                               gTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCCTAtt                                                   >  1:4003727/1‑41 (MQ=255)

                                               gTCGGTTCGTTGCCGTTGTTATGAAGCATCTCCAGCGTGTTGCGTGAGGTGCCACAGGCCGGGTTGTGa                       >  1:1025140/1‑69 (MQ=255)

                                                tCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATaa                    <  1:930599/71‑1 (MQ=255)

                                                tCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATaa                    <  1:1132343/71‑1 (MQ=255)

                                                tCGGTTCGTTGCCGTTGTTATGAAGCATCTCCAGCGTGTTGCGTGAGGTGCCACAGGCCGGGTTGTGATa                     <  1:3370188/70‑1 (MQ=255)

                                                 cGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTa                                                     >  1:4416215/1‑37 (MQ=255)

                                                  ggTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGt                            >  1:4083819/1‑61 (MQ=255)

                                                  ggTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAAt                  >  1:5296498/1‑71 (MQ=255)

                                                  ggTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAAt                  <  1:1609557/71‑1 (MQ=255)

                                                   gTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCg                                      <  1:3248690/50‑1 (MQ=255)

                                                   gTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCg                                      <  1:410810/50‑1 (MQ=255)

                                                   gTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCg                                      <  1:5081422/50‑1 (MQ=255)

                                                    ttCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATgg                >  1:5562081/1‑71 (MQ=255)

                                                    ttCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATgg                <  1:5046617/71‑1 (MQ=255)

                                                      cTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGt               >  1:2132299/1‑70 (MQ=255)

                                                      cTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGt               <  1:1929783/70‑1 (MQ=255)

                                                      cTGTGCCGCTGTTGCAGATCATCTCCAGCGTATTACGCGACGTGCCGCa                                    >  1:3609444/1‑49 (MQ=255)

                                                       tgtgCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGa                                             <  1:4425046/39‑1 (MQ=255)

                                                       tgtgCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCg                               <  1:2059901/53‑1 (MQ=255)

                                                       tgtgCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCg                               <  1:5660910/53‑1 (MQ=255)

                                                       gttgCCGTTGTTATGAAGCATCTCCAGCGTGTTGCGTGAGGTGCCACAGGCCGGGTTGTGATAGATGGTaa             <  1:3607979/69‑1 (MQ=255)

                                                        ttgCCGTTGTTATGAAGCATCTCCAGCGTGTTGCGTGAGGTGCCACAGGCCgg                              <  1:1573904/52‑1 (MQ=255)

                                                         tgCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCTGGTTGTGATAAATGGTAATg           <  1:68517/71‑1 (MQ=255)

                                                         tgCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTa              <  1:1049420/68‑1 (MQ=255)

                                                         tgCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTa              <  1:3335105/68‑1 (MQ=255)

                                                         tgCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATg           <  1:5096028/71‑1 (MQ=255)

                                                         tgCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATg           <  1:4748671/71‑1 (MQ=255)

                                                          gCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGt          >  1:3184184/1‑71 (MQ=255)

                                                           ccGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGtt         >  1:2829626/1‑71 (MQ=255)

                                                           ccGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGtt         >  1:5296390/1‑71 (MQ=255)

                                                            cGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTg        <  1:4973189/71‑1 (MQ=255)

                                                               tGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTtgt                         <  1:2512346/51‑1 (MQ=255)

                                                               tGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTtgt                         <  1:2260799/51‑1 (MQ=255)

                                                               tGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTtgt                         <  1:5333956/51‑1 (MQ=255)

                                                               tGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCt      >  1:2473706/1‑70 (MQ=255)

                                                                 ttGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATaa                    <  1:5669419/54‑1 (MQ=255)

                                                                 ttGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCat   <  1:3272102/71‑1 (MQ=255)

                                                                   gCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGc       >  1:613852/1‑65 (MQ=255)

                                                                   gCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGc       >  1:5166536/1‑65 (MQ=255)

TGACCAGTTCATCGCGCGTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATA  >  Exported/2595160‑2595296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`