BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	2,595,228	C→T	11.6%	R23H (CGC→CAC) ‡	arsC ←	arsenate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	2,595,228	0	C	T	11.6%	293.3 / 22.6	112	R23H (CGC→CAC) ‡	arsC	arsenate reductase
Reads supporting (aligned to +/- strand): ref base C (41/58); new base T (3/10); total (44/68)
Fisher's exact test for biased strand distribution p-value = 2.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.80e-01

Only 100 of 118 total aligned reads displayed.

TGACCAGTTCATCGCGCGTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATC  >  Exported/2595160‑2595298

tGACCAGTTCATCGCGCGTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCg                                                                       >  1:420386/1‑70 (MQ=255)

tGACCAGTTCATCGCGCGTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCg                                                                       >  1:3540696/1‑70 (MQ=255)

      gTTCATCGCGCGTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGatcat                                                                 >  1:5034946/1‑70 (MQ=255)

       ttCATCGCGCGTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGc                                                                        <  1:3169537/62‑1 (MQ=255)

       ttCATCGCGCGTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCAtct                                                               <  1:4188503/71‑1 (MQ=255)

       ttCATCGCGCGTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCAtct                                                               <  1:1235344/71‑1 (MQ=255)

         cATCGCGCGTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCgg                                                                      >  1:3305240/1‑62 (MQ=255)

         cATCGCGCGTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCgg                                                                      >  1:5314449/1‑62 (MQ=255)

         cATCGCGCGTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCAtctc                                                              >  1:156286/1‑70 (MQ=255)

         cATCGCGCGTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCAtctc                                                              >  1:3720909/1‑70 (MQ=255)

          aTCGCGCGTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCgg                                                                      >  1:398630/1‑61 (MQ=255)

            cgcgcgTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGc                                                                        <  1:3772907/57‑1 (MQ=255)

             gcgcgTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCg                                                         <  1:1662411/71‑1 (MQ=255)

               gcgTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTa                                                       <  1:4507579/71‑1 (MQ=255)

                cgTTGGCGGAGTTTCCAGATAATGGATAATAGTCTGTTCTGTGCCGCTGTTGCGGatc                                                                   <  1:5663473/58‑1 (MQ=255)

                cgTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGatc                                                                   <  1:1422093/58‑1 (MQ=255)

                cgTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTAt                                                      <  1:1505373/71‑1 (MQ=255)

                    ggCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTAc                                                   <  1:1590286/70‑1 (MQ=255)

                     gCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTAcgc                                                 <  1:2444658/71‑1 (MQ=255)

                     gCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTAcg                                                  <  1:772138/70‑1 (MQ=255)

                       ggAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGa                                               <  1:3161115/71‑1 (MQ=255)

                       ggAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGa                                               <  1:26413/71‑1 (MQ=255)

                         aGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGatcat                                                                 >  1:2306733/1‑51 (MQ=255)

                         aGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACg                                             >  1:214205/1‑71 (MQ=255)

                          gTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGt                                            <  1:3291164/71‑1 (MQ=255)

                           tttCCAGATAATAGATAATGGTCGGTTCTGTACCGCTGTTGCGGATCATCTCCAGCGTATTACGTGACGt                                            <  1:1088262/70‑1 (MQ=255)

                            ttCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCg                                                         <  1:4389756/56‑1 (MQ=255)

                            ttCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGAc                                              <  1:32204/67‑1 (MQ=255)

                             tcgaGATAATTAATTATTGTCGGTTCGTTGCCGTTGTTATGAAGCATCTCCAGCGTGTTGCGTGAGGTGc                                          >  1:3430818/4‑70 (MQ=255)

                             tCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGat                                                                    >  1:3112716/1‑44 (MQ=255)

                             tCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGc                                                          >  1:3515282/1‑54 (MQ=255)

                             tCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGc                                                          >  1:5279192/1‑54 (MQ=255)

                             tCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGcc                                         >  1:532932/1‑71 (MQ=255)

                              ccAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCg                                        >  1:2515271/1‑71 (MQ=255)

                               gaGATAATTAATTATTGTCGGTTCGTTGCCGTTGTTATGAAGCATCTCCAGCGTGTTGCGTGAGGTGcca                                        <  1:4622529/69‑2 (MQ=255)

                               gaGATAATTAATTATTGTCGGTTCGTTGCCGTTGTTATGAAGCATCTCCAGCGTGTTGCGTGAGGTGcca                                        <  1:1903951/69‑2 (MQ=255)

                               gaGATAATTAATTATTGTCGGTTCGTTGCCGTTGTTATGAAGCATCTCCAGCGTGTTGCGTGAGGTGcca                                        <  1:3918693/69‑2 (MQ=255)

                               cAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTAcgc                                                 <  1:2770303/61‑1 (MQ=255)

                                  aTAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCg                                                         <  1:2008825/50‑1 (MQ=255)

                                     aTGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCa                                      >  1:3825895/1‑66 (MQ=255)

                                     aTGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCa                                      >  1:4934723/1‑66 (MQ=255)

                                        gATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAg                                                           <  1:1751469/42‑1 (MQ=255)

                                        gATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAg                                                           <  1:2043411/42‑1 (MQ=255)

                                        gATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAg                                                           <  1:3691019/42‑1 (MQ=255)

                                        gATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAg                                                           <  1:3649309/42‑1 (MQ=255)

                                        gATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAg                                                           <  1:589468/42‑1 (MQ=255)

                                        gATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAg                                                           <  1:559705/42‑1 (MQ=255)

                                        gATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTa                                                       >  1:1638362/1‑46 (MQ=255)

                                        gATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTa                                                       >  1:631560/1‑46 (MQ=255)

                                        gATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGt                                            >  1:446952/1‑57 (MQ=255)

                                        gATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGt                              >  1:2619882/1‑71 (MQ=255)

                                         ataataGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGtt                             >  1:2615485/1‑71 (MQ=255)

                                          taataGTCGGTTCTGTGCC‑CTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTtgt                           <  1:552968/71‑1 (MQ=255)

                                            attgTCGGTTCGTTGCCGTTGTTATGAAGCATCTCCAGCGTGTTGCGTGAGGTGCCACAGGCCGGGTtgt                           >  1:765989/4‑70 (MQ=255)

                                            attgTCGGTTCGTTGCCGTTGTTATGAAGCATCTCCAGCGTGTTGCGTGAGGTGCCACAGGCCGGGTtgt                           <  1:2990478/67‑1 (MQ=255)

                                            ataGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTtgt                           <  1:1234973/70‑1 (MQ=255)

                                             taGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTa                                                    >  1:2270696/1‑44 (MQ=255)

                                             taGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGcc                                         <  1:3382434/55‑1 (MQ=255)

                                             taGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGcc                                         <  1:5451187/55‑1 (MQ=255)

                                               gTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTAtt                                                     >  1:5068052/1‑41 (MQ=255)

                                               gTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCCTAtt                                                     >  1:4003727/1‑41 (MQ=255)

                                               gTCGGTTCGTTGCCGTTGTTATGAAGCATCTCCAGCGTGTTGCGTGAGGTGCCACAGGCCGGGTTGTGa                         >  1:1025140/1‑69 (MQ=255)

                                                tCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATaa                      <  1:1132343/71‑1 (MQ=255)

                                                tCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATaa                      <  1:930599/71‑1 (MQ=255)

                                                 cGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTa                                                       >  1:4416215/1‑37 (MQ=255)

                                                 cGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATaaa                     >  1:5471374/1‑71 (MQ=255)

                                                  ggTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGt                              >  1:4083819/1‑61 (MQ=255)

                                                  ggTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAAt                    >  1:5296498/1‑71 (MQ=255)

                                                  ggTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAAt                    <  1:1609557/71‑1 (MQ=255)

                                                   gTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCg                                        <  1:410810/50‑1 (MQ=255)

                                                   gTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCg                                        <  1:3248690/50‑1 (MQ=255)

                                                   gTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCg                                        <  1:5081422/50‑1 (MQ=255)

                                                    ttCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATgg                  >  1:5562081/1‑71 (MQ=255)

                                                    ttCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATgg                  <  1:5046617/71‑1 (MQ=255)

                                                      cTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGt                 >  1:2132299/1‑70 (MQ=255)

                                                      cTGTGCCGCTGTTGCAGATCATCTCCAGCGTATTACGCGACGTGCCGCa                                      >  1:3609444/1‑49 (MQ=255)

                                                       tgtgCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCg                                 <  1:2059901/53‑1 (MQ=255)

                                                       tgtgCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCg                                 <  1:5660910/53‑1 (MQ=255)

                                                       gttgCCGTTGTTATGAAGCATCTCCAGCGTGTTGCGTGAGGTGCCACAGGCCGGGTTGTGATAGATGGTaa               <  1:3594924/69‑1 (MQ=255)

                                                        ttgCCGTTGTTATGAAGCATCTCCAGCGTGTTGCGTGAGGTGCCACAGGCCgg                                <  1:1573904/52‑1 (MQ=255)

                                                         tgCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCTGGTTGTGATAAATGGTAATg             <  1:68517/71‑1 (MQ=255)

                                                         tgCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGAt                        <  1:166696/60‑1 (MQ=255)

                                                         tgCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTa                <  1:1049420/68‑1 (MQ=255)

                                                         tgCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTa                <  1:3335105/68‑1 (MQ=255)

                                                         tgCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATg             <  1:5096028/71‑1 (MQ=255)

                                                         tgCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATg             <  1:4748671/71‑1 (MQ=255)

                                                          gCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGt            >  1:3184184/1‑71 (MQ=255)

                                                           ccGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGtt           >  1:2829626/1‑71 (MQ=255)

                                                           ccGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGtt           >  1:5296390/1‑71 (MQ=255)

                                                               tGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTtgt                           <  1:5333956/51‑1 (MQ=255)

                                                               tGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTtgt                           <  1:2260799/51‑1 (MQ=255)

                                                               tGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCt        >  1:2473706/1‑70 (MQ=255)

                                                                 ttGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATaa                      <  1:5669419/54‑1 (MQ=255)

                                                                 ttGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATaa                      <  1:4345273/54‑1 (MQ=255)

                                                                 ttGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCat     <  1:3272102/71‑1 (MQ=255)

                                                                  tGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCata    >  1:3934983/1‑71 (MQ=255)

                                                                   gCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGc         >  1:613852/1‑65 (MQ=255)

                                                                    cGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCatat   <  1:2095784/70‑1 (MQ=255)

                                                                    cGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCatat   <  1:928998/70‑1 (MQ=255)

                                                                    cGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATc  <  1:4791176/71‑1 (MQ=255)

TGACCAGTTCATCGCGCGTTGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATC  >  Exported/2595160‑2595298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`