Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,595,329 T→C 28.3% L423L (CTA→CTG) ‡ arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,595,3290TC28.3% 160.6 / 77.9 113L423L (CTA→CTG) ‡arsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base T (37/44);  new base C (17/15);  total (54/59)
Fisher's exact test for biased strand distribution p-value = 5.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 114 total aligned reads displayed.

CAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTA  >  Exported/2595261‑2595394
                                                                    |                                                                 
 aGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAgc                                                                >  1:61564/1‑71 (MQ=255)
   gCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAgcgc                                                              <  1:455331/71‑1 (MQ=255)
       ggTTGTGATAGATGGTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAgc                                                          <  1:2453903/71‑1 (MQ=255)
        gTTGTGATAGATGGTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAgc                                                          <  1:3826773/70‑1 (MQ=255)
         ttGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAgcgc                                                        <  1:340183/71‑1 (MQ=255)
          tgtgATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAgcgc                                                        <  1:4702643/70‑1 (MQ=255)
           gtgATAAATGCTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGca                                                       <  1:4087390/70‑1 (MQ=255)
             gATAGATGGTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGcggc                                                     >  1:4396535/1‑70 (MQ=255)
              aTAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAg                                                           >  1:3306303/1‑63 (MQ=255)
               tAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGcagccag                                                  <  1:2410854/71‑1 (MQ=255)
               tAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGcagcca                                                   <  1:574231/70‑1 (MQ=255)
                agaTGGTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAg                                                           <  1:5335149/59‑1 (MQ=255)
                aaaTGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGcagccagc                                                 >  1:3462899/1‑71 (MQ=255)
                aaaTGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGcagccagc                                                 >  1:3469632/1‑71 (MQ=255)
                aaaTGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGcagccagc                                                 >  1:3958727/1‑71 (MQ=255)
                 gaTGGTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGcggccaga                                                 <  1:3367669/69‑2 (MQ=255)
                  aTGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGt                                               <  1:5387995/71‑1 (MQ=255)
                  aTGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGt                                               >  1:3468783/1‑71 (MQ=255)
                  aTGGTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAgcg                                                         >  1:939264/1‑61 (MQ=255)
                  aTGGTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAgcg                                                         >  1:544843/1‑61 (MQ=255)
                   tGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTc                                              <  1:5228961/71‑1 (MQ=255)
                    ggTAATGTTGCTCATTTCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGtga                                             >  1:4247100/1‑69 (MQ=255)
                    ggTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGtga                                             >  1:4797135/1‑69 (MQ=255)
                       aaTGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTcaca                                           >  1:1251148/1‑70 (MQ=255)
                         tGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGcagc                                                     <  1:155170/58‑1 (MQ=255)
                          gTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTa                                                                  >  1:2370715/1‑44 (MQ=255)
                          gTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGcagc                                                     >  1:4469462/1‑57 (MQ=255)
                          gTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGcagc                                                     >  1:2162843/1‑57 (MQ=255)
                           ttGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGGGCAGCCAGCGTCACAAACAg                                      >  1:5265201/1‑71 (MQ=255)
                           ttGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAg                                      >  1:287879/1‑71 (MQ=255)
                           ttGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACa                                       <  1:3899196/70‑1 (MQ=255)
                           ttGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACa                                       <  1:5067645/70‑1 (MQ=255)
                           ttGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACa                                       <  1:1086304/70‑1 (MQ=255)
                              cTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGcc                                                             >  1:4304109/1‑45 (MQ=255)
                              cTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGcac                                   <  1:2572734/71‑1 (MQ=255)
                              cTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGcac                                   <  1:844464/71‑1 (MQ=255)
                              cTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGca                                    <  1:1061871/70‑1 (MQ=255)
                                cATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGcaccg                                 >  1:4169565/1‑69 (MQ=255)
                                 atatCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACa                                       <  1:2809995/64‑1 (MQ=255)
                                 atatCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACa                                       <  1:5688686/64‑1 (MQ=255)
                                 atatCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACa                                       <  1:2459377/64‑1 (MQ=255)
                                 atatCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACa                                       <  1:541780/64‑1 (MQ=255)
                                 atatCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcagg                                >  1:4622462/1‑71 (MQ=255)
                                  tatCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAgc                                                          <  1:5650733/44‑1 (MQ=255)
                                  tatCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGcggcca                                                   <  1:5087646/51‑1 (MQ=255)
                                  tatCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGcggcca                                                   <  1:4323806/51‑1 (MQ=255)
                                   atCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACa                                       >  1:2065172/1‑62 (MQ=255)
                                   atCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGcggcca                                                   >  1:2786411/1‑50 (MQ=255)
                                    tCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTc                                              >  1:2403905/1‑54 (MQ=255)
                                    tCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggcag                             >  1:4892038/1‑71 (MQ=255)
                                    tCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggca                              <  1:1831062/70‑1 (MQ=255)
                                    tCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggca                              <  1:1859326/70‑1 (MQ=255)
                                    tCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGcggccaga                                                 >  1:124132/1‑50 (MQ=255)
                                      aGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAggg                           <  1:977500/71‑1 (MQ=255)
                                        tATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGca                                    <  1:3053832/60‑1 (MQ=255)
                                          tctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTcaca                                           <  1:2242025/51‑1 (MQ=255)
                                          tctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACaaa                                         >  1:4709551/1‑53 (MQ=255)
                                          tctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAg                                      >  1:3134278/1‑56 (MQ=255)
                                          tctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAg                                      >  1:705235/1‑56 (MQ=255)
                                          tctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggcag                             <  1:4290259/65‑1 (MQ=255)
                                          tctaATTACAAAGTGACAGAGAGCCGCCACGCCAGCGcggccag                                                  <  1:2731350/44‑1 (MQ=12)
                                          tctaATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCAt                       >  1:1160607/1‑71 (MQ=255)
                                             aaTTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGa                     >  1:1664135/2‑70 (MQ=255)
                                              aTTACAAAGTGACAGAGAGCCGCCACGCCAgcgcg                                                       <  1:919605/35‑2 (MQ=12)
                                              aTTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGac                    <  1:3521973/70‑2 (MQ=255)
                                              aTTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGac                    >  1:4377325/1‑69 (MQ=255)
                                              aTTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGac                    >  1:1737157/1‑69 (MQ=255)
                                              aTTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATg                      <  1:2284194/68‑1 (MQ=255)
                                              aTTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGAt                    <  1:4851837/70‑1 (MQ=255)
                                              aTTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATa                   <  1:5540100/71‑1 (MQ=255)
                                               ttACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACa                                       <  1:4860878/50‑1 (MQ=255)
                                               ttACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATaa                  <  1:308626/71‑1 (MQ=255)
                                                tACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAAt                 >  1:5577178/1‑71 (MQ=255)
                                                tACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggcag                             <  1:5680226/59‑1 (MQ=255)
                                                 aCAAAGTGAAAGAGAGACGTAGCGCCAGCTCAGCCAGCGTCACAAAc                                        >  1:1909327/1‑47 (MQ=255)
                                                 aCAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGa                     >  1:4675637/1‑66 (MQ=255)
                                                 aCAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATc                >  1:2094823/1‑71 (MQ=255)
                                                 aCAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATc                >  1:2982070/1‑71 (MQ=255)
                                                  cAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAAtgc               <  1:3516961/71‑3 (MQ=255)
                                                  cAAAGTGAAAGAGAGACGTAGCGCCAGCTcagcca                                                   >  1:1783513/1‑35 (MQ=255)
                                                  cAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATcc               >  1:1603561/1‑71 (MQ=255)
                                                  cAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATcc               <  1:543292/71‑1 (MQ=255)
                                                   aaaGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGcac                                   <  1:4110322/50‑1 (MQ=255)
                                                   aaaGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGcac                                   <  1:1261978/50‑1 (MQ=255)
                                                    aaGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggcagg                            >  1:3923040/1‑56 (MQ=255)
                                                    aaGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggcagg                            >  1:2154353/1‑56 (MQ=255)
                                                     aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg            <  1:3202795/71‑1 (MQ=255)
                                                     aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg            <  1:4786190/71‑1 (MQ=255)
                                                     aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg            <  1:4669639/71‑1 (MQ=255)
                                                     aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg            <  1:1826622/71‑1 (MQ=255)
                                                      gTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATgccgg            >  1:5242204/1‑68 (MQ=255)
                                                      gTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg            >  1:1153276/1‑70 (MQ=255)
                                                      gTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGt           <  1:969119/71‑1 (MQ=255)
                                                           agagagACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATa                   >  1:2000060/1‑58 (MQ=255)
                                                              gagaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAg    >  1:825321/1‑70 (MQ=255)
                                                              gagaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAg    >  1:1827920/1‑70 (MQ=255)
                                                              gagaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGt   <  1:163546/71‑1 (MQ=255)
                                                              gagaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGt   <  1:1696360/71‑1 (MQ=255)
                                                               agaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTa  <  1:1321587/71‑1 (MQ=255)
                                                               agaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTa  <  1:5410098/71‑1 (MQ=255)
                                                                    |                                                                 
CAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTA  >  Exported/2595261‑2595394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: