Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,595,350 C→G 22.9% V416V (GTG→GTC arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,595,3500CG22.9% 211.7 / 59.0 109V416V (GTG→GTCarsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base C (34/50);  new base G (13/12);  total (47/62)
Fisher's exact test for biased strand distribution p-value = 3.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 114 total aligned reads displayed.

TGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTC  >  Exported/2595280‑2595419
                                                                      |                                                                     
tGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTc                                                                       <  1:5228961/71‑1 (MQ=255)
    aaTGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTcaca                                                                    >  1:1251148/1‑70 (MQ=255)
        ttGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGGGCAGCCAGCGTCACAAACAg                                                               >  1:5265201/1‑71 (MQ=255)
        ttGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAg                                                               >  1:287879/1‑71 (MQ=255)
        ttGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACa                                                                <  1:1086304/70‑1 (MQ=255)
        ttGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACa                                                                <  1:3899196/70‑1 (MQ=255)
        ttGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACa                                                                <  1:4281743/70‑1 (MQ=255)
        ttGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACa                                                                <  1:5067645/70‑1 (MQ=255)
           cTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGcac                                                            <  1:2572734/71‑1 (MQ=255)
           cTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGcac                                                            <  1:844464/71‑1 (MQ=255)
           cTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGca                                                             <  1:1061871/70‑1 (MQ=255)
             cATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGcaccg                                                          >  1:4169565/1‑69 (MQ=255)
              atatCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACa                                                                <  1:541780/64‑1 (MQ=255)
              atatCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACa                                                                <  1:5688686/64‑1 (MQ=255)
              atatCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACa                                                                <  1:2459377/64‑1 (MQ=255)
              atatCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACa                                                                <  1:2809995/64‑1 (MQ=255)
              atatCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcagg                                                         >  1:4622462/1‑71 (MQ=255)
                atCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACa                                                                >  1:2065172/1‑62 (MQ=255)
                 tCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTc                                                                       >  1:2403905/1‑54 (MQ=255)
                 tCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggcag                                                      >  1:4892038/1‑71 (MQ=255)
                 tCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggca                                                       <  1:1831062/70‑1 (MQ=255)
                   aGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAggg                                                    <  1:977500/71‑1 (MQ=255)
                     tATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGca                                                             <  1:3053832/60‑1 (MQ=255)
                       tctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTcaca                                                                    <  1:2242025/51‑1 (MQ=255)
                       tctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACaaa                                                                  >  1:4709551/1‑53 (MQ=255)
                       tctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAg                                                               >  1:3134278/1‑56 (MQ=255)
                       tctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAg                                                               >  1:705235/1‑56 (MQ=255)
                       tctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggcag                                                      <  1:4290259/65‑1 (MQ=255)
                       tctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggcag                                                      <  1:3355540/65‑1 (MQ=255)
                       tctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCAt                                                >  1:65953/1‑71 (MQ=255)
                       tctaATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCAccggcaca                                                     >  1:3592595/1‑64 (MQ=255)
                       tctaATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCAt                                                >  1:1160607/1‑71 (MQ=255)
                       tctaATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCAt                                                >  1:1518678/1‑71 (MQ=255)
                          aaTTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGa                                              >  1:1664135/2‑70 (MQ=255)
                           aTTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGac                                             <  1:3521973/70‑2 (MQ=255)
                           aTTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGac                                             >  1:4377325/1‑69 (MQ=255)
                           aTTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGac                                             >  1:1737157/1‑69 (MQ=255)
                           aTTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATg                                               <  1:2284194/68‑1 (MQ=255)
                           aTTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGAt                                             <  1:4851837/70‑1 (MQ=255)
                           aTTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATa                                            <  1:5540100/71‑1 (MQ=255)
                            ttACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACa                                                                <  1:4860878/50‑1 (MQ=255)
                            ttACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATaa                                           <  1:308626/71‑1 (MQ=255)
                             tACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAAt                                          >  1:5577178/1‑71 (MQ=255)
                             tACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggcag                                                      <  1:5680226/59‑1 (MQ=255)
                              aCAAAGTGAAAGAGAGACGTAGCGCCAGCTCAGCCAGCGTCACAAAc                                                                 >  1:1909327/1‑47 (MQ=255)
                              aCAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGa                                              >  1:4675637/1‑66 (MQ=255)
                              aCAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATc                                         >  1:2982070/1‑71 (MQ=255)
                              aCAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATc                                         >  1:2094823/1‑71 (MQ=255)
                               cAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATcc                                        <  1:543292/71‑1 (MQ=255)
                               cAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATcc                                        >  1:1603561/1‑71 (MQ=255)
                                aaaGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGcac                                                            <  1:1261978/50‑1 (MQ=255)
                                aaaGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGcac                                                            <  1:4110322/50‑1 (MQ=255)
                                 aaGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggcagg                                                     >  1:2154353/1‑56 (MQ=255)
                                 aaGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggcagg                                                     >  1:3923040/1‑56 (MQ=255)
                                  aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg                                     <  1:4786190/71‑1 (MQ=255)
                                  aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg                                     <  1:3202795/71‑1 (MQ=255)
                                  aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg                                     <  1:1826622/71‑1 (MQ=255)
                                   gTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATgccgg                                     >  1:5242204/1‑68 (MQ=255)
                                   gTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg                                     >  1:1153276/1‑70 (MQ=255)
                                   gTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGt                                    <  1:969119/71‑1 (MQ=255)
                                        agagagACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATa                                            >  1:2000060/1‑58 (MQ=255)
                                           gagaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAg                             >  1:825321/1‑70 (MQ=255)
                                           gagaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAg                             >  1:1827920/1‑70 (MQ=255)
                                           gagaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGt                            <  1:163546/71‑1 (MQ=255)
                                           gagaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGt                            <  1:5453997/71‑1 (MQ=255)
                                            agaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTa                           <  1:5410098/71‑1 (MQ=255)
                                            agaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTa                           <  1:1321587/71‑1 (MQ=255)
                                                 tAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggc                                                        <  1:1791825/37‑1 (MQ=255)
                                                  cacgcCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATcc                     >  1:5377867/3‑71 (MQ=255)
                                                  aGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAAt                                          >  1:1784388/1‑50 (MQ=255)
                                                  aGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAAt                                          >  1:5171048/1‑50 (MQ=255)
                                                   gcgcCCGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCAt                                                >  1:1979192/1‑43 (MQ=255)
                                                   gcgcCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCAt                                                >  1:83857/1‑43 (MQ=255)
                                                   gcgcCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGa                                              >  1:3131452/1‑45 (MQ=255)
                                                   gcgcCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATccc                    >  1:5127962/1‑71 (MQ=255)
                                                   gcgcCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATccc                    >  1:5591103/1‑71 (MQ=255)
                                                   acgcCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATccc                    >  1:2590656/2‑71 (MQ=255)
                                                    cgcCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATcccc                   <  1:3471919/71‑1 (MQ=255)
                                                      ccAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAg                 >  1:4473558/1‑71 (MQ=255)
                                                         gcgcGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATcccc                   <  1:4704743/66‑1 (MQ=255)
                                                         gcgcAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCt               >  1:2839713/1‑70 (MQ=255)
                                                          cgcAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa             <  1:29933/71‑1 (MQ=255)
                                                          cgcAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa             <  1:513441/71‑1 (MQ=255)
                                                          cgcAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa             <  1:1243161/71‑1 (MQ=255)
                                                            cagccagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCt                                      <  1:2041747/44‑1 (MQ=255)
                                                            cagccagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa             >  1:5070701/1‑69 (MQ=255)
                                                            cagccagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa             >  1:3649646/1‑69 (MQ=255)
                                                             ggccagaGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGAttg          >  1:3870623/2‑69 (MQ=255)
                                                              gccagaGTGACAAACAGCACCGGCACAGTCATGac                                             <  1:2503828/35‑2 (MQ=12)
                                                              gccagaGTGACAAACAGCACCGGCACAGTCATGac                                             <  1:584133/35‑2 (MQ=12)
                                                                cagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCa       <  1:214073/71‑1 (MQ=255)
                                                                 agcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCa       <  1:1674147/70‑1 (MQ=255)
                                                                 agcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCa       >  1:5646237/1‑70 (MQ=255)
                                                                 agcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCa       <  1:396040/70‑1 (MQ=255)
                                                                   cGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCatat    <  1:1256084/71‑1 (MQ=255)
                                                                    gTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCatat    <  1:2985680/70‑1 (MQ=255)
                                                                     tCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa             <  1:4270512/60‑1 (MQ=255)
                                                                     tCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa             <  1:1595552/60‑1 (MQ=255)
                                                                     tCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttc  <  1:1791542/71‑1 (MQ=255)
                                                                      cacaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTc        >  1:5575767/1‑64 (MQ=255)
                                                                      |                                                                     
TGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTC  >  Exported/2595280‑2595419

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: