Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,595,410 A→T 29.5% T396T (ACT→ACA arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,595,4100AT29.5% 204.8 / 104.8 132T396T (ACT→ACAarsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base A (50/43);  new base T (17/22);  total (67/65)
Fisher's exact test for biased strand distribution p-value = 3.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 137 total aligned reads displayed.

CAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCG  >  Exported/2595344‑2595480
                                                                  |                                                                      
cagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGCTAGTCa                                                                    <  1:5555/71‑1 (MQ=255)
cagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCa                                                                    <  1:214073/71‑1 (MQ=255)
  gcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCata                                                                  <  1:4893225/71‑1 (MQ=255)
   cGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCatat                                                                 <  1:4148128/71‑1 (MQ=255)
   cGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCatat                                                                 <  1:614030/71‑1 (MQ=255)
    gTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCatat                                                                 <  1:2985680/70‑1 (MQ=255)
     tgacaAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATAttt                                                               <  1:1203956/69‑2 (MQ=255)
     tCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttc                                                               <  1:1791542/71‑1 (MQ=255)
       acaAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATAttttt                                                             <  1:3852905/71‑1 (MQ=255)
       acaAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATAtttt                                                              <  1:2152288/70‑3 (MQ=255)
       acaAACAGCAAAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttctt                                                             >  1:2334813/1‑71 (MQ=255)
         aaaCAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATttttct                                                           >  1:760969/1‑71 (MQ=255)
         aaaCAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATttttct                                                           >  1:4119604/1‑71 (MQ=255)
           aCAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGc                                                         >  1:4643370/1‑71 (MQ=255)
            cagcagAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGc                                                         >  1:5555800/1‑70 (MQ=255)
             aGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTggg                                                        >  1:4332141/1‑68 (MQ=255)
             aGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTggg                                                        >  1:74608/1‑68 (MQ=255)
                acaGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATtcttct                                                           >  1:34713/1‑64 (MQ=255)
                  aggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGt                                                                      <  1:2549641/51‑1 (MQ=255)
                  aggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTa                                                  >  1:1731795/1‑71 (MQ=255)
                   ggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTAc                                                 <  1:1130225/71‑1 (MQ=255)
                    gcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACg                                                <  1:5252685/71‑1 (MQ=255)
                      aggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCata                                                                  >  1:2803758/1‑51 (MQ=255)
                      acagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCAc                                                 <  1:1809577/65‑1 (MQ=255)
                      acagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg                                              <  1:5285263/68‑1 (MQ=255)
                      acagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg                                              >  1:1389504/4‑71 (MQ=255)
                       gggTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGc                                             <  1:1524422/71‑1 (MQ=255)
                       cagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCa                                                                    <  1:3979752/46‑1 (MQ=16)
                       cagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGc                                             <  1:52761/69‑1 (MQ=255)
                        tgTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTAc                                                 <  1:2257996/65‑1 (MQ=255)
                        ggTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttc                                                               <  1:3415756/52‑1 (MQ=255)
                          tCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGaa                                                      <  1:5615276/59‑1 (MQ=255)
                          tCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGc                                          >  1:4912920/1‑71 (MQ=255)
                          tCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAg                                                    >  1:774505/1‑61 (MQ=255)
                          tCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAg                                                    >  1:4417615/1‑61 (MQ=255)
                           cATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATAtt                                                                >  1:5272059/1‑48 (MQ=19)
                           cATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGc                                          >  1:3535977/1‑70 (MQ=255)
                            aTGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTc                                                                     >  1:431553/1‑42 (MQ=255)
                            aTGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTc                                                                     >  1:4134779/1‑42 (MQ=255)
                            aTGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCa                                        <  1:203005/71‑1 (MQ=255)
                             tGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCata                                                                  <  1:4453964/44‑1 (MQ=255)
                             tGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCata                                                                  <  1:3012696/44‑1 (MQ=255)
                               aTAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttctt                                                             >  1:4746272/1‑47 (MQ=255)
                               aTAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttctt                                                             >  1:2099194/1‑47 (MQ=255)
                               aTAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCagag                                     <  1:5564881/71‑1 (MQ=255)
                                tAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGt                                                                      >  1:700295/1‑37 (MQ=255)
                                tAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAg                                                    >  1:5424356/1‑55 (MQ=255)
                                tAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAg                                                    >  1:4243826/1‑55 (MQ=255)
                                   tCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagc                                 >  1:4444805/1‑71 (MQ=255)
                                    cccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgta                                   >  1:516239/1‑68 (MQ=255)
                                    cccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgta                                   >  1:4924942/1‑68 (MQ=255)
                                     ccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcg                                >  1:4551306/1‑70 (MQ=255)
                                      cggTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAgcagagtt                              <  1:5530447/69‑2 (MQ=255)
                                       tGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgtag                             >  1:549829/1‑71 (MQ=255)
                                        gTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgtagc                            >  1:4700516/1‑71 (MQ=255)
                                        gTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCAcat                                               <  1:2890320/52‑3 (MQ=255)
                                         tACGGAAGTAATATCCCCAGCTGATAGTGATATTCTTCTGCGAAAGTACGTGCAGCCa                                        <  1:5601061/58‑1 (MQ=255)
                                         tACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTa                                                  >  1:4353467/1‑48 (MQ=255)
                                         tACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCa                                        <  1:1988895/58‑1 (MQ=255)
                                         tACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgtag                             <  1:846704/69‑1 (MQ=255)
                                          accgAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGcc                           <  1:4689893/67‑1 (MQ=255)
                                          aCGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAgcagagttgc                            >  1:4250600/1‑69 (MQ=255)
                                           cGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAg                         >  1:1359458/1‑71 (MQ=255)
                                              aaGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGc                       >  1:1061447/1‑70 (MQ=255)
                                              aaGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGc                       >  1:4607899/1‑70 (MQ=255)
                                              aaGTAATATCCCCAAGTGATTGTCATATTTTTCTTGGCAAGCACATGCAGCCac                                       <  1:3114598/54‑2 (MQ=255)
                                              aaGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCac                                       <  1:4735523/54‑2 (MQ=255)
                                               aGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACtg                     >  1:2813062/1‑70 (MQ=255)
                                               aGTAATATCCCCAAGTGATTGTCATATTTTTCAGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACt                      <  1:878483/70‑1 (MQ=255)
                                                gTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg                                              <  1:1365411/45‑1 (MQ=255)
                                                gTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACtgc                    <  1:1763288/71‑3 (MQ=255)
                                                 tAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACtgc                    >  1:3296041/1‑68 (MQ=255)
                                                  aaTATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCa                                            >  1:3256388/1‑45 (MQ=255)
                                                   atatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcg                                >  1:2705721/1‑56 (MQ=255)
                                                   atatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAg                         >  1:5683652/1‑63 (MQ=255)
                                                   atatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCaa                 >  1:2033974/1‑71 (MQ=255)
                                                    tatCCCCAGCTGATAGTCATATTCTTCTGCGaaat                                                    <  1:367818/35‑2 (MQ=255)
                                                    tatCCCCAGCTGATAGTCATATTCTTCTGCGAAAg                                                    <  1:4743152/35‑1 (MQ=255)
                                                    tatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCATAGTAGCGTAGCCAGGCTACCaa                 <  1:4347204/70‑1 (MQ=255)
                                                    tatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAAt                <  1:2615888/71‑1 (MQ=255)
                                                     atCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAAtt               >  1:2975719/1‑71 (MQ=255)
                                                      tCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTg              >  1:3338200/1‑71 (MQ=255)
                                                       ccccAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTgg             >  1:4586071/1‑71 (MQ=255)
                                                        cccAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTggg            >  1:4068552/1‑71 (MQ=255)
                                                        cccAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTggg            >  1:1531737/1‑71 (MQ=255)
                                                          cAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCaga                                      <  1:1632740/43‑1 (MQ=255)
                                                          cAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCaga                                      <  1:262241/43‑1 (MQ=255)
                                                          cAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCaga                                      <  1:1476712/43‑1 (MQ=255)
                                                           aGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGt          >  1:2497030/1‑70 (MQ=255)
                                                            gCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagc                                 >  1:1134345/1‑46 (MQ=255)
                                                            gCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTaa        <  1:2839072/71‑1 (MQ=255)
                                                            gCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTaa        <  1:1272417/71‑1 (MQ=255)
                                                              tGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCTAGGCTACCa                  <  1:2456311/59‑1 (MQ=255)
                                                                aTAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgtagc                            >  1:124800/1‑47 (MQ=255)
                                                                aTAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAAtt      <  1:4232981/69‑1 (MQ=255)
                                                                 tAGTCATATTCTTCTGCGAAAGTACGTGCAGCCagag                                     <  1:1191807/37‑1 (MQ=255)
                                                                  aGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgta                              >  1:1767616/1‑43 (MQ=255)
                                                                  aGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgta                              >  1:2896609/1‑43 (MQ=255)
                                                                  aGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCg  >  1:3583917/1‑71 (MQ=255)
                                                                  aGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCg  >  1:3891123/1‑71 (MQ=255)
                                                                  |                                                                      
CAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCG  >  Exported/2595344‑2595480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: