Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,595,425 C→G 21.5% S391S (TCG→TCC) ‡ arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,595,4250CG21.5% 240.9 / 60.5 121S391S (TCG→TCC) ‡arsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base C (58/37);  new base G (10/16);  total (68/53)
Fisher's exact test for biased strand distribution p-value = 4.68e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 127 total aligned reads displayed.

ACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAG  >  Exported/2595355‑2595494
                                                                      |                                                                     
aCAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGc                                                                       >  1:4643370/1‑71 (MQ=255)
 cagcagAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGc                                                                       >  1:5555800/1‑70 (MQ=255)
       aggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTa                                                                >  1:1731795/1‑71 (MQ=255)
        ggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTAc                                                               <  1:4430395/71‑1 (MQ=255)
         gcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACg                                                              <  1:5252685/71‑1 (MQ=255)
          caggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGt                                                             <  1:4120927/71‑1 (MQ=255)
           acagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg                                                            <  1:555069/68‑1 (MQ=255)
           acagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg                                                            <  1:5285263/68‑1 (MQ=255)
           acagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg                                                            >  1:1389504/4‑71 (MQ=255)
            gggTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGc                                                           <  1:1524422/71‑1 (MQ=255)
            cagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg                                                            <  1:2483560/68‑1 (MQ=255)
            cagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGc                                                           <  1:725182/69‑1 (MQ=255)
             tgTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTAc                                                               <  1:2257996/65‑1 (MQ=255)
               tCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGaa                                                                    <  1:5615276/59‑1 (MQ=255)
               tCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAg                                                                  >  1:774505/1‑61 (MQ=255)
               tCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAg                                                                  >  1:4417615/1‑61 (MQ=255)
               tCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAg                                                                  >  1:2554648/1‑61 (MQ=255)
                cATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGc                                                        >  1:3535977/1‑70 (MQ=255)
                 aTGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCa                                                      <  1:5321996/71‑1 (MQ=255)
                 aTGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg                                                            >  1:833614/1‑65 (MQ=255)
                 aTGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCa                                                      <  1:203005/71‑1 (MQ=255)
                     tAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAg                                                                  >  1:5424356/1‑55 (MQ=255)
                     tAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAg                                                                  >  1:470386/1‑55 (MQ=255)
                     tAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAg                                                                  >  1:4243826/1‑55 (MQ=255)
                         cccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgta                                                 >  1:516239/1‑68 (MQ=255)
                         cccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgta                                                 >  1:5274802/1‑68 (MQ=255)
                         cccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgta                                                 >  1:2801405/1‑68 (MQ=255)
                         cccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgta                                                 >  1:4924942/1‑68 (MQ=255)
                          ccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcg                                              >  1:4551306/1‑70 (MQ=255)
                           cggTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAgcagagtt                                            <  1:5530447/69‑2 (MQ=255)
                            tGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgtag                                           >  1:3436204/1‑71 (MQ=255)
                              tACGGAAGTAATATCCCCAGCTGATAGTGATATTCTTCTGCGAAAGTACGTGCAGCCa                                                      <  1:5601061/58‑1 (MQ=255)
                              tACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTa                                                                >  1:4353467/1‑48 (MQ=255)
                              tACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCa                                                      <  1:1988895/58‑1 (MQ=255)
                              tACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgtag                                           <  1:846704/69‑1 (MQ=255)
                               accgAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGcc                                         <  1:4689893/67‑1 (MQ=255)
                               aCGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAgcagagttgc                                          >  1:4250600/1‑69 (MQ=255)
                                cGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAg                                       >  1:1359458/1‑71 (MQ=255)
                                   aaGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGc                                     >  1:4607899/1‑70 (MQ=255)
                                   aaGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGc                                     >  1:1061447/1‑70 (MQ=255)
                                   aaGTAATATCCCCAAGTGATTGTCATATTTTTCTTGGCAAGCACATGCAGCCac                                                     <  1:3114598/54‑2 (MQ=255)
                                   aaGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCac                                                     <  1:4735523/54‑2 (MQ=255)
                                    aGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACtg                                   >  1:2813062/1‑70 (MQ=255)
                                    aGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACt                                    <  1:1140045/70‑1 (MQ=255)
                                    aGTAATATCCCCAAGTGATTGTCATATTTTTCAGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACt                                    <  1:878483/70‑1 (MQ=255)
                                     gTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg                                                            <  1:457075/45‑1 (MQ=255)
                                     gTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg                                                            <  1:1365411/45‑1 (MQ=255)
                                     gTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACtgc                                  <  1:1763288/71‑3 (MQ=255)
                                      tAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACtgc                                  >  1:3296041/1‑68 (MQ=255)
                                        atatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAgg                                      >  1:292915/1‑64 (MQ=255)
                                        atatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAg                                       >  1:5683652/1‑63 (MQ=255)
                                        atatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCaa                               >  1:2033974/1‑71 (MQ=255)
                                         tatCCCCAGCTGATAGTCATATTCTTCTGCGAAAg                                                                  <  1:4743152/35‑1 (MQ=255)
                                         tatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAAt                              <  1:2615888/71‑1 (MQ=255)
                                           tCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTg                            >  1:3338200/1‑71 (MQ=255)
                                             cccAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTggg                          >  1:4068552/1‑71 (MQ=255)
                                             cccAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTggg                          >  1:1531737/1‑71 (MQ=255)
                                               cAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCaga                                                    <  1:1632740/43‑1 (MQ=255)
                                               cAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCaga                                                    <  1:1476712/43‑1 (MQ=255)
                                               cAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCaga                                                    <  1:262241/43‑1 (MQ=255)
                                                 gCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagc                                               >  1:1134345/1‑46 (MQ=255)
                                                 gCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTaa                      <  1:2839072/71‑1 (MQ=255)
                                                 gCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTaa                      <  1:1272417/71‑1 (MQ=255)
                                                   tGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCTAGGCTACCa                                <  1:2456311/59‑1 (MQ=255)
                                                      tAGTCATATTCTTCTGCGAAAGTACGTGCAGCCagag                                                   <  1:1191807/37‑1 (MQ=255)
                                                       aGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgta                                            >  1:1767616/1‑43 (MQ=255)
                                                       aGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgta                                            >  1:2896609/1‑43 (MQ=255)
                                                       aGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAAt                              >  1:4653318/1‑57 (MQ=255)
                                                       aGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCg                >  1:3891123/1‑71 (MQ=255)
                                                       aGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCg                >  1:3583917/1‑71 (MQ=255)
                                                        gTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCg                <  1:5434796/70‑1 (MQ=255)
                                                         tCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCggg              <  1:1234240/71‑2 (MQ=255)
                                                           atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCa                                        >  1:1007220/1‑43 (MQ=255)
                                                           atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAAttt                   >  1:979338/1‑64 (MQ=255)
                                                           atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTc                 >  1:866379/1‑66 (MQ=255)
                                                           atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTc                 >  1:61438/1‑66 (MQ=255)
                                                           atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTc                 >  1:2489482/1‑66 (MQ=255)
                                                           atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTc                 >  1:3968983/1‑66 (MQ=255)
                                                           atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCg                >  1:1747697/1‑67 (MQ=255)
                                                           atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTcc            >  1:4548297/1‑71 (MQ=255)
                                                           atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTcc            >  1:3141656/1‑71 (MQ=255)
                                                           atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTcc            >  1:3251462/1‑71 (MQ=255)
                                                            tatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTcc            >  1:3336725/1‑70 (MQ=255)
                                                            tatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTcc            >  1:4974575/1‑70 (MQ=255)
                                                              ttcttcTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCaa         <  1:4267265/71‑1 (MQ=255)
                                                              ttcttcTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCaa         <  1:5141489/71‑1 (MQ=255)
                                                               tcttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCa                                        <  1:4705840/39‑1 (MQ=255)
                                                               tcttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCa                                <  1:1290473/47‑1 (MQ=255)
                                                               tcttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTccc           >  1:1469863/1‑68 (MQ=255)
                                                                cttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCaaa        <  1:4285372/70‑1 (MQ=255)
                                                                 ttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGc                                     <  1:1569405/40‑1 (MQ=255)
                                                                 ttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGc                                     >  1:1011515/1‑40 (MQ=255)
                                                                 ttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGc                                     <  1:1002196/40‑1 (MQ=255)
                                                                     gCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAg  >  1:3120286/1‑71 (MQ=255)
                                                                     gCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAg  >  1:2687148/1‑71 (MQ=255)
                                                                     gCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAg  >  1:4940678/1‑71 (MQ=255)
                                                                      cGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCt                                    >  1:4534146/1‑36 (MQ=255)
                                                                      cGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGc    <  1:2964023/68‑1 (MQ=255)
                                                                      cGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGc    <  1:2793191/68‑1 (MQ=255)
                                                                      cGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGc    <  1:3526813/68‑1 (MQ=255)
                                                                      |                                                                     
ACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAG  >  Exported/2595355‑2595494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: