Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,595,431 T→C 17.4% V389V (GTA→GTG arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,595,4310TC17.4% 287.6 / 49.5 132V389V (GTA→GTGarsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base T (66/43);  new base C (7/16);  total (73/59)
Fisher's exact test for biased strand distribution p-value = 1.09e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

Only 100 of 137 total aligned reads displayed.

AGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCA  >  Exported/2595362‑2595501
                                                                     |                                                                      
aggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTa                                                                       >  1:1731795/1‑71 (MQ=255)
 ggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTAc                                                                      <  1:1130225/71‑1 (MQ=255)
   caggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGt                                                                    <  1:1486229/71‑1 (MQ=255)
    acagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCAc                                                                      <  1:1809577/65‑1 (MQ=255)
    acagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg                                                                   <  1:5285263/68‑1 (MQ=255)
    acagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg                                                                   >  1:1389504/4‑71 (MQ=255)
     cagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg                                                                   <  1:2483560/68‑1 (MQ=255)
     cagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGc                                                                  <  1:725182/69‑1 (MQ=255)
     cagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGc                                                                  <  1:52761/69‑1 (MQ=255)
      tgTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTAc                                                                      <  1:2257996/65‑1 (MQ=255)
        tCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGc                                                               >  1:4912920/1‑71 (MQ=255)
          aTGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg                                                                   >  1:833614/1‑65 (MQ=255)
          aTGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCa                                                             <  1:203005/71‑1 (MQ=255)
             aTAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCagag                                                          <  1:5564881/71‑1 (MQ=255)
                 tCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagc                                                      >  1:4444805/1‑71 (MQ=255)
                  cccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgta                                                        >  1:2801405/1‑68 (MQ=255)
                  cccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgta                                                        >  1:516239/1‑68 (MQ=255)
                  cccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgta                                                        >  1:5274802/1‑68 (MQ=255)
                   ccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcg                                                     >  1:4551306/1‑70 (MQ=255)
                    cggTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAgcagagtt                                                   <  1:5530447/69‑2 (MQ=255)
                     tGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgtag                                                  >  1:549829/1‑71 (MQ=255)
                     tGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgtag                                                  >  1:3436204/1‑71 (MQ=255)
                       tACGGAAGTAATATCCCCAGCTGATAGTGATATTCTTCTGCGAAAGTACGTGCAGCCa                                                             <  1:5601061/58‑1 (MQ=255)
                       tACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTa                                                                       >  1:1377180/1‑48 (MQ=255)
                       tACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTa                                                                       >  1:4353467/1‑48 (MQ=255)
                       tACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCa                                                             <  1:1988895/58‑1 (MQ=255)
                       tACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgtag                                                  <  1:846704/69‑1 (MQ=255)
                        accgAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGcc                                                <  1:4689893/67‑1 (MQ=255)
                        aCGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAgcagagttgc                                                 >  1:4250600/1‑69 (MQ=255)
                          ggAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAgg                                             >  1:2068162/1‑71 (MQ=255)
                            aaGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGc                                            >  1:4607899/1‑70 (MQ=255)
                            aaGTAATATCCCCAAGTGATTGTCATATTTTTCTTGGCAAGCACATGCAGCCac                                                            <  1:3114598/54‑2 (MQ=255)
                            aaGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCac                                                            <  1:4735523/54‑2 (MQ=255)
                             aGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACtg                                          >  1:2813062/1‑70 (MQ=255)
                             aGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACt                                           <  1:1140045/70‑1 (MQ=255)
                             aGTAATATCCCCAAGTGATTGTCATATTTTTCAGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACt                                           <  1:878483/70‑1 (MQ=255)
                              gTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg                                                                   <  1:457075/45‑1 (MQ=255)
                              gTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg                                                                   <  1:1365411/45‑1 (MQ=255)
                              gTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACtgc                                         <  1:1763288/71‑3 (MQ=255)
                               tAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACtgc                                         >  1:3296041/1‑68 (MQ=255)
                                aaTATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCa                                                                 >  1:3256388/1‑45 (MQ=255)
                                 atatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAgg                                             >  1:292915/1‑64 (MQ=255)
                                 atatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCa                                       <  1:5022952/70‑1 (MQ=255)
                                  tatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCATAGTAGCGTAGCCAGGCTACCaa                                      <  1:4347204/70‑1 (MQ=255)
                                  tatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAAt                                     <  1:2615888/71‑1 (MQ=255)
                                      cccAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTggg                                 >  1:1531737/1‑71 (MQ=255)
                                      cccAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTggg                                 >  1:4068552/1‑71 (MQ=255)
                                        cAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCaga                                                           <  1:1632740/43‑1 (MQ=255)
                                        cAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCaga                                                           <  1:262241/43‑1 (MQ=255)
                                        cAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCaga                                                           <  1:1476712/43‑1 (MQ=255)
                                          gCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagc                                                      >  1:1134345/1‑46 (MQ=255)
                                          gCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTaa                             <  1:2839072/71‑1 (MQ=255)
                                            tGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCTAGGCTACCa                                       <  1:2456311/59‑1 (MQ=255)
                                              aTAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgtagc                                                 >  1:124800/1‑47 (MQ=255)
                                              aTAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAAtt                           <  1:4232981/69‑1 (MQ=255)
                                                aGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgta                                                   >  1:1767616/1‑43 (MQ=255)
                                                aGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgta                                                   >  1:2896609/1‑43 (MQ=255)
                                                aGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAAt                                     >  1:4653318/1‑57 (MQ=255)
                                                aGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCg                       >  1:3583917/1‑71 (MQ=255)
                                                aGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCg                       >  1:3891123/1‑71 (MQ=255)
                                                 gTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCg                       <  1:5434796/70‑1 (MQ=255)
                                                  tCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCggg                     <  1:1234240/71‑2 (MQ=255)
                                                    atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCa                                               >  1:1007220/1‑43 (MQ=255)
                                                    atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCa                                               >  1:3477133/1‑43 (MQ=255)
                                                    atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAAttt                          >  1:979338/1‑64 (MQ=255)
                                                    atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTc                        >  1:2489482/1‑66 (MQ=255)
                                                    atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTc                        >  1:61438/1‑66 (MQ=255)
                                                    atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTcc                   >  1:3251462/1‑71 (MQ=255)
                                                    atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTcc                   >  1:3141656/1‑71 (MQ=255)
                                                     tatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTcc                   >  1:3336725/1‑70 (MQ=255)
                                                     tatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTcc                   >  1:4974575/1‑70 (MQ=255)
                                                        tcttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCa                                               <  1:4705840/39‑1 (MQ=255)
                                                        tcttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCa                                       <  1:1290473/47‑1 (MQ=255)
                                                        tcttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTccc                  >  1:1699749/1‑68 (MQ=255)
                                                         cttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCaaa               <  1:4285372/70‑1 (MQ=255)
                                                          ttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGc                                            >  1:1011515/1‑40 (MQ=255)
                                                          ttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGc                                            <  1:1002196/40‑1 (MQ=255)
                                                          ttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGc                                            <  1:1569405/40‑1 (MQ=255)
                                                          ttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATc             >  1:696216/1‑71 (MQ=255)
                                                           tctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCg            >  1:855357/1‑71 (MQ=255)
                                                              gCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAg         >  1:3120286/1‑71 (MQ=255)
                                                              gCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAg         >  1:4940678/1‑71 (MQ=255)
                                                              gCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAg         >  1:2687148/1‑71 (MQ=255)
                                                               cGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCt                                           >  1:4534146/1‑36 (MQ=255)
                                                               cGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGc           <  1:2964023/68‑1 (MQ=255)
                                                               cGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGc           <  1:3526813/68‑1 (MQ=255)
                                                                gAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATc             <  1:3178037/65‑1 (MQ=255)
                                                                 aaaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCa      <  1:1218542/71‑1 (MQ=255)
                                                                  aaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCgg                      >  1:1273099/1‑54 (MQ=255)
                                                                  aaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCg                       >  1:3799103/1‑53 (MQ=255)
                                                                  aaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCaa     <  1:4062936/71‑1 (MQ=255)
                                                                  aaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACAAATTGGGGTAATTTTc                        >  1:1483323/1‑52 (MQ=255)
                                                                   aGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAAt    >  1:4120768/1‑71 (MQ=255)
                                                                   aGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAAt    >  1:3615097/1‑71 (MQ=255)
                                                                    gTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCaa                                      >  1:1796600/1‑36 (MQ=255)
                                                                    gTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCaa                                      >  1:4497734/1‑36 (MQ=255)
                                                                     tACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAAt                            <  1:5153649/45‑1 (MQ=255)
                                                                     tACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATca  <  1:2745415/71‑1 (MQ=255)
                                                                     tACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATca  <  1:2751941/71‑1 (MQ=255)
                                                                     tACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATca  >  1:3863892/1‑71 (MQ=255)
                                                                     |                                                                      
AGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCA  >  Exported/2595362‑2595501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: