Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,595,434 G→A 16.4% H388H (CAC→CAT arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,595,4340GA16.4% 296.8 / 42.6 128H388H (CAC→CATarsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base G (65/42);  new base A (7/14);  total (72/56)
Fisher's exact test for biased strand distribution p-value = 2.94e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.64e-01

Only 100 of 131 total aligned reads displayed.

GCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACAT  >  Exported/2595364‑2595504
                                                                      |                                                                      
gcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACg                                                                        <  1:5252685/71‑1 (MQ=255)
 caggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGt                                                                       <  1:1486229/71‑1 (MQ=255)
  acagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg                                                                      <  1:5285263/68‑1 (MQ=255)
  acagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg                                                                      <  1:555069/68‑1 (MQ=255)
  acagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg                                                                      >  1:1389504/4‑71 (MQ=255)
   gggTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGc                                                                     <  1:1524422/71‑1 (MQ=255)
   cagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg                                                                      <  1:2483560/68‑1 (MQ=255)
   cagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGc                                                                     <  1:52761/69‑1 (MQ=255)
      tCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGc                                                                  >  1:4912920/1‑71 (MQ=255)
       cATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGc                                                                  >  1:3535977/1‑70 (MQ=255)
        aTGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCa                                                                <  1:5321996/71‑1 (MQ=255)
        aTGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg                                                                      >  1:833614/1‑65 (MQ=255)
        aTGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCa                                                                <  1:203005/71‑1 (MQ=255)
           aTAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCagag                                                             <  1:5564881/71‑1 (MQ=255)
               tCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagc                                                         >  1:4444805/1‑71 (MQ=255)
                cccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgta                                                           >  1:516239/1‑68 (MQ=255)
                cccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgta                                                           >  1:2801405/1‑68 (MQ=255)
                 ccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcg                                                        >  1:4551306/1‑70 (MQ=255)
                  cggTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAgcagagtt                                                      <  1:5530447/69‑2 (MQ=255)
                   tGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgtag                                                     >  1:3436204/1‑71 (MQ=255)
                    gTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgtagc                                                    >  1:4700516/1‑71 (MQ=255)
                     tACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCa                                                                <  1:1988895/58‑1 (MQ=255)
                     tACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgtag                                                     <  1:846704/69‑1 (MQ=255)
                      accgAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGcc                                                   <  1:4689893/67‑1 (MQ=255)
                      aCGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAgcagagttgc                                                    >  1:4250600/1‑69 (MQ=255)
                       cGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAg                                                 >  1:1359458/1‑71 (MQ=255)
                        ggAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAgg                                                >  1:2068162/1‑71 (MQ=255)
                          aaGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGc                                               >  1:4607899/1‑70 (MQ=255)
                          aaGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGc                                               >  1:1061447/1‑70 (MQ=255)
                          aaGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCac                                                               <  1:4735523/54‑2 (MQ=255)
                           aGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACtg                                             >  1:2813062/1‑70 (MQ=255)
                           aGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACt                                              <  1:1140045/70‑1 (MQ=255)
                           aGTAATATCCCCAAGTGATTGTCATATTTTTCAGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACt                                              <  1:878483/70‑1 (MQ=255)
                            gTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg                                                                      <  1:457075/45‑1 (MQ=255)
                            gTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg                                                                      <  1:1365411/45‑1 (MQ=255)
                            gTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACtgc                                            <  1:1763288/71‑3 (MQ=255)
                             tAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACtgc                                            >  1:3296041/1‑68 (MQ=255)
                              aaTATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCa                                                                    >  1:3256388/1‑45 (MQ=255)
                               atatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcg                                                        >  1:2705721/1‑56 (MQ=255)
                               atatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAgg                                                >  1:292915/1‑64 (MQ=255)
                               atatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAg                                                 >  1:5683652/1‑63 (MQ=255)
                               atatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCa                                          <  1:5022952/70‑1 (MQ=255)
                                tatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCATAGTAGCGTAGCCAGGCTACCaa                                         <  1:4347204/70‑1 (MQ=255)
                                tatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAAt                                        <  1:2615888/71‑1 (MQ=255)
                                 atCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAAtt                                       >  1:2975719/1‑71 (MQ=255)
                                  tCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTg                                      >  1:3338200/1‑71 (MQ=255)
                                    cccAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTggg                                    >  1:4068552/1‑71 (MQ=255)
                                      cAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCaga                                                              <  1:262241/43‑1 (MQ=255)
                                      cAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCaga                                                              <  1:1476712/43‑1 (MQ=255)
                                      cAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCaga                                                              <  1:1632740/43‑1 (MQ=255)
                                       aGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGt                                  >  1:2497030/1‑70 (MQ=255)
                                        gCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTaa                                <  1:2839072/71‑1 (MQ=255)
                                        gCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTaa                                <  1:1272417/71‑1 (MQ=255)
                                          tGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCTAGGCTACCa                                          <  1:2456311/59‑1 (MQ=255)
                                            aTAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgtagc                                                    >  1:124800/1‑47 (MQ=255)
                                            aTAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAAtt                              <  1:4232981/69‑1 (MQ=255)
                                             tAGTCATATTCTTCTGCGAAAGTACGTGCAGCCagag                                                             <  1:1191807/37‑1 (MQ=255)
                                              aGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgta                                                      >  1:1767616/1‑43 (MQ=255)
                                              aGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAAt                                        >  1:4653318/1‑57 (MQ=255)
                                              aGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCg                          >  1:3583917/1‑71 (MQ=255)
                                              aGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCg                          >  1:3891123/1‑71 (MQ=255)
                                               gTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCg                          <  1:5434796/70‑1 (MQ=255)
                                                  atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCa                                                  >  1:1007220/1‑43 (MQ=255)
                                                  atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCa                                                  >  1:3477133/1‑43 (MQ=255)
                                                  atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAAttt                             >  1:979338/1‑64 (MQ=255)
                                                  atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTc                           >  1:866379/1‑66 (MQ=255)
                                                  atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTc                           >  1:61438/1‑66 (MQ=255)
                                                  atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCg                          >  1:1747697/1‑67 (MQ=255)
                                                  atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTcc                      >  1:4548297/1‑71 (MQ=255)
                                                  atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTcc                      >  1:3141656/1‑71 (MQ=255)
                                                   tatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTcc                      >  1:4974575/1‑70 (MQ=255)
                                                     ttcttcTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCaa                   <  1:4267265/71‑1 (MQ=255)
                                                        ttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGc                                               <  1:1002196/40‑1 (MQ=255)
                                                        ttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGc                                               >  1:1011515/1‑40 (MQ=255)
                                                        ttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGc                                               <  1:1569405/40‑1 (MQ=255)
                                                        ttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATc                >  1:696216/1‑71 (MQ=255)
                                                            gCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAg            >  1:4940678/1‑71 (MQ=255)
                                                            gCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAg            >  1:3120286/1‑71 (MQ=255)
                                                             cGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGc              <  1:2964023/68‑1 (MQ=255)
                                                              gAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATc                <  1:3178037/65‑1 (MQ=255)
                                                              gAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGc           >  1:3121949/1‑70 (MQ=255)
                                                               aaaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCa         <  1:1218542/71‑1 (MQ=255)
                                                               aaaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCa         <  1:1807966/71‑1 (MQ=255)
                                                                aaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTa                                 >  1:2402409/1‑46 (MQ=255)
                                                                aaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCgg                         >  1:1273099/1‑54 (MQ=255)
                                                                aaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCg                          >  1:3799103/1‑53 (MQ=255)
                                                                aaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCaa        <  1:3251813/71‑1 (MQ=255)
                                                                aaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCaa        <  1:4062936/71‑1 (MQ=255)
                                                                aaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCaa        <  1:1582911/71‑1 (MQ=255)
                                                                aaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACAAATTGGGGTAATTTTc                           >  1:1483323/1‑52 (MQ=255)
                                                                 aGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAAt       >  1:3615097/1‑71 (MQ=255)
                                                                 aGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAAt       >  1:4120768/1‑71 (MQ=255)
                                                                  gTACGTTCAGCCAGAGTAGCGTAGCCAGGCTACCaa                                         >  1:732879/1‑36 (MQ=255)
                                                                  gTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCaa                                         >  1:4497734/1‑36 (MQ=255)
                                                                   tACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAAt                               <  1:5153649/45‑1 (MQ=255)
                                                                   tACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATca     <  1:2745415/71‑1 (MQ=255)
                                                                   tACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATca     >  1:3863892/1‑71 (MQ=255)
                                                                   tACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATca     <  1:2751941/71‑1 (MQ=255)
                                                                      gTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACAt  <  1:4778729/71‑1 (MQ=255)
                                                                      gTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACAt  <  1:3790476/71‑1 (MQ=255)
                                                                      |                                                                      
GCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACAT  >  Exported/2595364‑2595504

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: