Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,595,443 G→C 9.1% L385L (CTC→CTG arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,595,4430GC 9.1% 311.1 / 16.4 110L385L (CTC→CTGarsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base G (66/33);  new base C (5/5);  total (71/39)
Fisher's exact test for biased strand distribution p-value = 3.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 120 total aligned reads displayed.

ATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAA  >  Exported/2595375‑2595512
                                                                    |                                                                     
aTAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCagag                                                                     <  1:5564881/71‑1 (MQ=255)
     cccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgta                                                                   >  1:5274802/1‑68 (MQ=255)
     cccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgta                                                                   >  1:516239/1‑68 (MQ=255)
     cccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgta                                                                   >  1:4924942/1‑68 (MQ=255)
     cccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgta                                                                   >  1:2801405/1‑68 (MQ=255)
      ccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcg                                                                >  1:4551306/1‑70 (MQ=255)
        tGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgtag                                                             >  1:3436204/1‑71 (MQ=255)
        tGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgtag                                                             >  1:549829/1‑71 (MQ=255)
         gTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgtagc                                                            >  1:4700516/1‑71 (MQ=255)
          tACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgtag                                                             <  1:846704/69‑1 (MQ=255)
           accgAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGcc                                                           <  1:4689893/67‑1 (MQ=255)
           aCGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAgcagagttgc                                                            >  1:4250600/1‑69 (MQ=255)
            cGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAg                                                         >  1:1359458/1‑71 (MQ=255)
             ggAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAgg                                                        >  1:2068162/1‑71 (MQ=255)
               aaGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGc                                                       >  1:1061447/1‑70 (MQ=255)
                aGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACtg                                                     >  1:2813062/1‑70 (MQ=255)
                aGTAATATCCCCAAGTGATTGTCATATTTTTCAGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACt                                                      <  1:878483/70‑1 (MQ=255)
                 gTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACtgc                                                    <  1:1763288/71‑3 (MQ=255)
                  tAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACtgc                                                    >  1:3296041/1‑68 (MQ=255)
                    atatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcg                                                                >  1:2705721/1‑56 (MQ=255)
                    atatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAgg                                                        >  1:292915/1‑64 (MQ=255)
                    atatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAg                                                         >  1:5683652/1‑63 (MQ=255)
                    atatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCa                                                  <  1:5022952/70‑1 (MQ=255)
                     tatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCATAGTAGCGTAGCCAGGCTACCaa                                                 <  1:4347204/70‑1 (MQ=255)
                     tatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAAt                                                <  1:2615888/71‑1 (MQ=255)
                      atCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAAtt                                               >  1:2975719/1‑71 (MQ=255)
                        ccccAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTgg                                             >  1:4586071/1‑71 (MQ=255)
                         cccAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTggg                                            >  1:1531737/1‑71 (MQ=255)
                         cccAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTggg                                            >  1:4068552/1‑71 (MQ=255)
                           cAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCaga                                                                      <  1:1632740/43‑1 (MQ=255)
                           cAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCaga                                                                      <  1:262241/43‑1 (MQ=255)
                           cAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCaga                                                                      <  1:1476712/43‑1 (MQ=255)
                            aGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGt                                          >  1:2497030/1‑70 (MQ=255)
                             gCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagc                                                                 >  1:1134345/1‑46 (MQ=255)
                             gCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTaa                                        <  1:2839072/71‑1 (MQ=255)
                             gCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTaa                                        <  1:1272417/71‑1 (MQ=255)
                               tGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCTAGGCTACCa                                                  <  1:2456311/59‑1 (MQ=255)
                                 aTAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgtagc                                                            >  1:124800/1‑47 (MQ=255)
                                 aTAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAAtt                                      <  1:4232981/69‑1 (MQ=255)
                                  tAGTCATATTCTTCTGCGAAAGTACGTGCAGCCagag                                                                     <  1:1191807/37‑1 (MQ=255)
                                   aGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgta                                                              >  1:1767616/1‑43 (MQ=255)
                                   aGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAAt                                                >  1:4653318/1‑57 (MQ=255)
                                   aGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCg                                  >  1:3891123/1‑71 (MQ=255)
                                   aGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCg                                  >  1:3583917/1‑71 (MQ=255)
                                    gTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCg                                  <  1:5434796/70‑1 (MQ=255)
                                     tCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCggg                                <  1:1234240/71‑2 (MQ=255)
                                       atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCa                                                          >  1:3477133/1‑43 (MQ=255)
                                       atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCa                                                          >  1:1007220/1‑43 (MQ=255)
                                       atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAAttt                                     >  1:979338/1‑64 (MQ=255)
                                       atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTc                                   >  1:2489482/1‑66 (MQ=255)
                                       atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTc                                   >  1:866379/1‑66 (MQ=255)
                                       atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTc                                   >  1:61438/1‑66 (MQ=255)
                                       atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTc                                   >  1:3968983/1‑66 (MQ=255)
                                       atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTcc                              >  1:4548297/1‑71 (MQ=255)
                                       atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTcc                              >  1:3251462/1‑71 (MQ=255)
                                        tatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTcc                              >  1:3336725/1‑70 (MQ=255)
                                        tatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTcc                              >  1:4974575/1‑70 (MQ=255)
                                          ttcttcTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCaa                           <  1:5141489/71‑1 (MQ=255)
                                          ttcttcTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCaa                           <  1:4267265/71‑1 (MQ=255)
                                           tcttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCa                                                          <  1:4705840/39‑1 (MQ=255)
                                           tcttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCa                                                  <  1:1290473/47‑1 (MQ=255)
                                           tcttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTccc                             >  1:1699749/1‑68 (MQ=255)
                                           tcttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTccc                             >  1:1469863/1‑68 (MQ=255)
                                            cttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCaaa                          <  1:4285372/70‑1 (MQ=255)
                                             ttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGc                                                       >  1:1011515/1‑40 (MQ=255)
                                             ttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGc                                                       <  1:1569405/40‑1 (MQ=255)
                                             ttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGc                                                       <  1:1002196/40‑1 (MQ=255)
                                             ttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATc                        >  1:696216/1‑71 (MQ=255)
                                              tctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCg                       >  1:855357/1‑71 (MQ=255)
                                                 gCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAg                    >  1:3120286/1‑71 (MQ=255)
                                                 gCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAg                    >  1:4940678/1‑71 (MQ=255)
                                                 gCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAg                    >  1:2687148/1‑71 (MQ=255)
                                                  cGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCt                                                      >  1:4534146/1‑36 (MQ=255)
                                                  cGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGc                      <  1:2793191/68‑1 (MQ=255)
                                                  cGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGc                      <  1:2964023/68‑1 (MQ=255)
                                                  cGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGc                      <  1:3526813/68‑1 (MQ=255)
                                                   gAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATc                        <  1:3178037/65‑1 (MQ=255)
                                                   gAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGc                   >  1:3121949/1‑70 (MQ=255)
                                                    aaaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCa                 <  1:1218542/71‑1 (MQ=255)
                                                    aaaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCa                 <  1:1807966/71‑1 (MQ=255)
                                                     aaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTa                                         >  1:2402409/1‑46 (MQ=255)
                                                     aaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCg                                  >  1:3799103/1‑53 (MQ=255)
                                                     aaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCaa                <  1:3251813/71‑1 (MQ=255)
                                                     aaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCaa                <  1:1582911/71‑1 (MQ=255)
                                                     aaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCaa                <  1:4062936/71‑1 (MQ=255)
                                                     aaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACAAATTGGGGTAATTTTc                                   >  1:1483323/1‑52 (MQ=255)
                                                       gTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCaa                                                 >  1:1796600/1‑36 (MQ=255)
                                                       gTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCaa                                                 >  1:4497734/1‑36 (MQ=255)
                                                        tACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCaa                                                 >  1:3714587/1‑35 (MQ=255)
                                                        tACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATca             <  1:2751941/71‑1 (MQ=255)
                                                        tACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATca             >  1:3863892/1‑71 (MQ=255)
                                                           gTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACAt          <  1:3790476/71‑1 (MQ=255)
                                                           gTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACAt          <  1:4778729/71‑1 (MQ=255)
                                                           atGCAGCCACAGCAGAGTTGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACAt          <  1:3814260/70‑1 (MQ=255)
                                                            tGCAGCCACAGCAGAGTTGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACAt          >  1:665170/1‑70 (MQ=255)
                                                             gCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTg        >  1:528571/1‑71 (MQ=255)
                                                                gCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACAtt         <  1:3803179/67‑1 (MQ=255)
                                                                  cAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATa   <  1:1972875/71‑1 (MQ=255)
                                                                   agagTAGCGTAGCCAGGCTACCAATTGGGGTAAtttt                                    <  1:3498099/37‑1 (MQ=255)
                                                                   agagTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATa   >  1:2716251/1‑70 (MQ=255)
                                                                    |                                                                     
ATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAA  >  Exported/2595375‑2595512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: