Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,595,446 T→C 8.1% L384L (CTA→CTG arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,595,4460TC 8.1% 276.4 / 11.0 99L384L (CTA→CTGarsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base T (54/37);  new base C (4/4);  total (58/41)
Fisher's exact test for biased strand distribution p-value = 7.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

Only 100 of 120 total aligned reads displayed.

TCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAAC  >  Exported/2595379‑2595514
                                                                   |                                                                    
tCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagc                                                                   >  1:4444805/1‑71 (MQ=255)
 cccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgta                                                                     >  1:4924942/1‑68 (MQ=255)
 cccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgta                                                                     >  1:516239/1‑68 (MQ=255)
 cccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgta                                                                     >  1:2801405/1‑68 (MQ=255)
 cccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgta                                                                     >  1:5274802/1‑68 (MQ=255)
  ccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcg                                                                  >  1:4551306/1‑70 (MQ=255)
   cggTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAgcagagtt                                                                <  1:5530447/69‑2 (MQ=255)
    tGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgtag                                                               >  1:3436204/1‑71 (MQ=255)
    tGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgtag                                                               >  1:549829/1‑71 (MQ=255)
      tACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgtag                                                               <  1:846704/69‑1 (MQ=255)
       accgAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGcc                                                             <  1:4689893/67‑1 (MQ=255)
       aCGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAgcagagttgc                                                              >  1:4250600/1‑69 (MQ=255)
        cGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAg                                                           >  1:1359458/1‑71 (MQ=255)
         ggAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAgg                                                          >  1:2068162/1‑71 (MQ=255)
           aaGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGc                                                         >  1:4607899/1‑70 (MQ=255)
           aaGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGc                                                         >  1:1061447/1‑70 (MQ=255)
            aGTAATATCCCCAAGTGATTGTCATATTTTTCAGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACt                                                        <  1:878483/70‑1 (MQ=255)
             gTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACtgc                                                      <  1:1763288/71‑3 (MQ=255)
                atatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAgg                                                          >  1:292915/1‑64 (MQ=255)
                atatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAg                                                           >  1:5683652/1‑63 (MQ=255)
                atatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCaa                                                   >  1:2033974/1‑71 (MQ=255)
                atatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCa                                                    <  1:5022952/70‑1 (MQ=255)
                 tatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCATAGTAGCGTAGCCAGGCTACCaa                                                   <  1:4347204/70‑1 (MQ=255)
                 tatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAAt                                                  <  1:2615888/71‑1 (MQ=255)
                  atCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAAtt                                                 >  1:2975719/1‑71 (MQ=255)
                   tCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTg                                                >  1:3338200/1‑71 (MQ=255)
                    ccccAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTgg                                               >  1:4586071/1‑71 (MQ=255)
                     cccAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTggg                                              >  1:1531737/1‑71 (MQ=255)
                     cccAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTggg                                              >  1:4068552/1‑71 (MQ=255)
                        aGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGt                                            >  1:2497030/1‑70 (MQ=255)
                         gCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagc                                                                   >  1:1134345/1‑46 (MQ=255)
                         gCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTaa                                          <  1:1272417/71‑1 (MQ=255)
                         gCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTaa                                          <  1:2839072/71‑1 (MQ=255)
                             aTAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgtagc                                                              >  1:124800/1‑47 (MQ=255)
                             aTAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAAtt                                        <  1:4232981/69‑1 (MQ=255)
                               aGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgta                                                                >  1:2896609/1‑43 (MQ=255)
                               aGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgta                                                                >  1:1767616/1‑43 (MQ=255)
                               aGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAAt                                                  >  1:4653318/1‑57 (MQ=255)
                               aGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCg                                    >  1:3891123/1‑71 (MQ=255)
                               aGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCg                                    >  1:3583917/1‑71 (MQ=255)
                                gTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCg                                    <  1:5434796/70‑1 (MQ=255)
                                 tCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCggg                                  <  1:1234240/71‑2 (MQ=255)
                                   atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCa                                                            >  1:3477133/1‑43 (MQ=255)
                                   atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCa                                                            >  1:1007220/1‑43 (MQ=255)
                                   atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTc                                     >  1:2489482/1‑66 (MQ=255)
                                   atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTc                                     >  1:866379/1‑66 (MQ=255)
                                   atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTc                                     >  1:61438/1‑66 (MQ=255)
                                   atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCg                                    >  1:1747697/1‑67 (MQ=255)
                                   atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTcc                                >  1:3251462/1‑71 (MQ=255)
                                   atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTcc                                >  1:3141656/1‑71 (MQ=255)
                                    tatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTcc                                >  1:3336725/1‑70 (MQ=255)
                                      ttcttcTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCaa                             <  1:5141489/71‑1 (MQ=255)
                                       tcttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCa                                                            <  1:4705840/39‑1 (MQ=255)
                                       tcttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTccc                               >  1:1469863/1‑68 (MQ=255)
                                       tcttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTccc                               >  1:1699749/1‑68 (MQ=255)
                                        cttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCaaa                            <  1:4285372/70‑1 (MQ=255)
                                         ttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGc                                                         >  1:1011515/1‑40 (MQ=255)
                                         ttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGc                                                         <  1:1002196/40‑1 (MQ=255)
                                         ttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATc                          >  1:696216/1‑71 (MQ=255)
                                          tctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCg                         >  1:855357/1‑71 (MQ=255)
                                             gCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAg                      >  1:4940678/1‑71 (MQ=255)
                                             gCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAg                      >  1:2687148/1‑71 (MQ=255)
                                              cGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCt                                                        >  1:4534146/1‑36 (MQ=255)
                                              cGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGc                        <  1:2964023/68‑1 (MQ=255)
                                              cGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGc                        <  1:2793191/68‑1 (MQ=255)
                                               gAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATc                          <  1:3178037/65‑1 (MQ=255)
                                               gAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGc                     >  1:3121949/1‑70 (MQ=255)
                                                aaaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCa                   <  1:1218542/71‑1 (MQ=255)
                                                aaaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCa                   <  1:1807966/71‑1 (MQ=255)
                                                 aaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTa                                           >  1:2402409/1‑46 (MQ=255)
                                                 aaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCgg                                   >  1:1273099/1‑54 (MQ=255)
                                                 aaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCg                                    >  1:3799103/1‑53 (MQ=255)
                                                 aaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCaa                  <  1:3251813/71‑1 (MQ=255)
                                                 aaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCaa                  <  1:4062936/71‑1 (MQ=255)
                                                 aaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCaa                  <  1:1582911/71‑1 (MQ=255)
                                                 aaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACAAATTGGGGTAATTTTc                                     >  1:1483323/1‑52 (MQ=255)
                                                  aGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAAt                 >  1:3615097/1‑71 (MQ=255)
                                                  aGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAAt                 >  1:4120768/1‑71 (MQ=255)
                                                   gTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCaa                                                   >  1:4497734/1‑36 (MQ=255)
                                                    tACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCaa                                                   >  1:3714587/1‑35 (MQ=255)
                                                    tACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAAt                                         <  1:5153649/45‑1 (MQ=255)
                                                    tACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATca               <  1:2745415/71‑1 (MQ=255)
                                                    tACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATca               <  1:2751941/71‑1 (MQ=255)
                                                    tACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATca               >  1:3863892/1‑71 (MQ=255)
                                                     acatGCAGCCACAGCAGAGTTGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATca               >  1:1514912/4‑70 (MQ=255)
                                                       gTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACAt            <  1:4778729/71‑1 (MQ=255)
                                                       gTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACAt            <  1:3790476/71‑1 (MQ=255)
                                                       atGCAGCCACAGCAGAGTTGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACAt            <  1:3814260/70‑1 (MQ=255)
                                                        tGCAGCCACAGCAGAGTTGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACAt            >  1:665170/1‑70 (MQ=255)
                                                         gCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTg          >  1:528571/1‑71 (MQ=255)
                                                           aGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTgg         >  1:2543930/1‑70 (MQ=255)
                                                              cAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATa     <  1:1972875/71‑1 (MQ=255)
                                                               agagTAGCGTAGCCAGGCTACCAATTGGGGTAAtttt                                      <  1:3498099/37‑1 (MQ=255)
                                                               agagTAGCGTAGCCAGGCTACCAATTGGGGTAAttt                                       >  1:3833663/1‑36 (MQ=255)
                                                               agagTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATaa    <  1:4839785/71‑1 (MQ=255)
                                                               agagTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATa     >  1:2716251/1‑70 (MQ=255)
                                                                 agTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAAc  <  1:3884190/71‑1 (MQ=255)
                                                                   tagcgtagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCa                              <  1:507659/41‑1 (MQ=255)
                                                                   tagcgtagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCa                              <  1:698790/41‑1 (MQ=255)
                                                                   tagcgtagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCa                              <  1:892936/41‑1 (MQ=255)
                                                                   |                                                                    
TCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAAC  >  Exported/2595379‑2595514

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: