Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,595,452 A→T 8.1% A382A (GCT→GCA arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,595,4520AT 8.1% 273.9 / 13.4 99A382A (GCT→GCAarsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base A (54/37);  new base T (4/4);  total (58/41)
Fisher's exact test for biased strand distribution p-value = 7.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.37e-01

Only 100 of 118 total aligned reads displayed.

TGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTT  >  Exported/2595383‑2595522
                                                                     |                                                                      
 gTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgtagc                                                                      >  1:4700516/1‑71 (MQ=255)
  tACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgtag                                                                       <  1:846704/69‑1 (MQ=255)
   accgAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGcc                                                                     <  1:4689893/67‑1 (MQ=255)
   aCGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAgcagagttgc                                                                      >  1:4250600/1‑69 (MQ=255)
    cGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAg                                                                   >  1:1359458/1‑71 (MQ=255)
     ggAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAgg                                                                  >  1:2068162/1‑71 (MQ=255)
       aaGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGc                                                                 >  1:4607899/1‑70 (MQ=255)
       aaGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGc                                                                 >  1:1061447/1‑70 (MQ=255)
        aGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACtg                                                               >  1:2813062/1‑70 (MQ=255)
        aGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACt                                                                <  1:1140045/70‑1 (MQ=255)
        aGTAATATCCCCAAGTGATTGTCATATTTTTCAGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACt                                                                <  1:878483/70‑1 (MQ=255)
         gTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACtgc                                                              <  1:1763288/71‑3 (MQ=255)
          tAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACtgc                                                              >  1:3296041/1‑68 (MQ=255)
            atatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAgg                                                                  >  1:292915/1‑64 (MQ=255)
            atatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAg                                                                   >  1:5683652/1‑63 (MQ=255)
            atatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCaa                                                           >  1:2033974/1‑71 (MQ=255)
            atatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCa                                                            <  1:5022952/70‑1 (MQ=255)
             tatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCATAGTAGCGTAGCCAGGCTACCaa                                                           <  1:4347204/70‑1 (MQ=255)
             tatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAAt                                                          <  1:2615888/71‑1 (MQ=255)
              atCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAAtt                                                         >  1:2975719/1‑71 (MQ=255)
               tCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTg                                                        >  1:3338200/1‑71 (MQ=255)
                ccccAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTgg                                                       >  1:4586071/1‑71 (MQ=255)
                 cccAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTggg                                                      >  1:4068552/1‑71 (MQ=255)
                 cccAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTggg                                                      >  1:1531737/1‑71 (MQ=255)
                    aGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGt                                                    >  1:2497030/1‑70 (MQ=255)
                     gCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTaa                                                  <  1:2839072/71‑1 (MQ=255)
                     gCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTaa                                                  <  1:1272417/71‑1 (MQ=255)
                       tGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCTAGGCTACCa                                                            <  1:2456311/59‑1 (MQ=255)
                         aTAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgtagc                                                                      >  1:124800/1‑47 (MQ=255)
                           aGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgta                                                                        >  1:1767616/1‑43 (MQ=255)
                           aGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgta                                                                        >  1:2896609/1‑43 (MQ=255)
                           aGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAAt                                                          >  1:4653318/1‑57 (MQ=255)
                           aGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCg                                            >  1:3583917/1‑71 (MQ=255)
                            gTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCg                                            <  1:5434796/70‑1 (MQ=255)
                             tCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCggg                                          <  1:1234240/71‑2 (MQ=255)
                               atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCa                                                                    >  1:3477133/1‑43 (MQ=255)
                               atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCa                                                                    >  1:1007220/1‑43 (MQ=255)
                               atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTc                                             >  1:3968983/1‑66 (MQ=255)
                               atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTc                                             >  1:866379/1‑66 (MQ=255)
                               atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTc                                             >  1:61438/1‑66 (MQ=255)
                               atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCg                                            >  1:1747697/1‑67 (MQ=255)
                               atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTcc                                        >  1:3141656/1‑71 (MQ=255)
                               atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTcc                                        >  1:3251462/1‑71 (MQ=255)
                                tatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTcc                                        >  1:4974575/1‑70 (MQ=255)
                                tatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTcc                                        >  1:3336725/1‑70 (MQ=255)
                                  ttcttcTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCaa                                     <  1:4267265/71‑1 (MQ=255)
                                   tcttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCa                                                            <  1:1290473/47‑1 (MQ=255)
                                    cttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCaaa                                    <  1:4285372/70‑1 (MQ=255)
                                     ttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGc                                                                 >  1:1011515/1‑40 (MQ=255)
                                     ttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGc                                                                 <  1:1002196/40‑1 (MQ=255)
                                     ttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGc                                                                 <  1:1569405/40‑1 (MQ=255)
                                     ttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATc                                  >  1:696216/1‑71 (MQ=255)
                                      tctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCg                                 >  1:855357/1‑71 (MQ=255)
                                         gCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAg                              >  1:2687148/1‑71 (MQ=255)
                                         gCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAg                              >  1:4940678/1‑71 (MQ=255)
                                         gCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAg                              >  1:3120286/1‑71 (MQ=255)
                                          cGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCt                                                                >  1:4534146/1‑36 (MQ=255)
                                          cGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGc                                <  1:2793191/68‑1 (MQ=255)
                                          cGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGc                                <  1:3526813/68‑1 (MQ=255)
                                           gAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGc                             >  1:3121949/1‑70 (MQ=255)
                                            aaaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCa                           <  1:1807966/71‑1 (MQ=255)
                                             aaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTa                                                   >  1:2402409/1‑46 (MQ=255)
                                             aaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCgg                                           >  1:1273099/1‑54 (MQ=255)
                                             aaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCg                                            >  1:3799103/1‑53 (MQ=255)
                                             aaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCaa                          <  1:4062936/71‑1 (MQ=255)
                                             aaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCaa                          <  1:3251813/71‑1 (MQ=255)
                                             aaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCaa                          <  1:1582911/71‑1 (MQ=255)
                                             aaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACAAATTGGGGTAATTTTc                                             >  1:1483323/1‑52 (MQ=255)
                                              aGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAAt                         >  1:4120768/1‑71 (MQ=255)
                                              aGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAAt                         >  1:3615097/1‑71 (MQ=255)
                                               gTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCaa                                                           >  1:1796600/1‑36 (MQ=255)
                                               gTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCaa                                                           >  1:4497734/1‑36 (MQ=255)
                                                tACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCaa                                                           >  1:3714587/1‑35 (MQ=255)
                                                tACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAAt                                                 <  1:5153649/45‑1 (MQ=255)
                                                tACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATca                       <  1:2745415/71‑1 (MQ=255)
                                                tACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATca                       >  1:3863892/1‑71 (MQ=255)
                                                 acatGCAGCCACAGCAGAGTTGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATca                       >  1:1514912/4‑70 (MQ=255)
                                                   gTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACAt                    <  1:4778729/71‑1 (MQ=255)
                                                   atGCAGCCACAGCAGAGTTGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACAt                    <  1:3814260/70‑1 (MQ=255)
                                                    tGCAGCCACAGCAGAGTTGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACAt                    >  1:665170/1‑70 (MQ=255)
                                                     gCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTg                  >  1:528571/1‑71 (MQ=255)
                                                       aGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTgg                 >  1:2543930/1‑70 (MQ=255)
                                                        gCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACAtt                   <  1:3803179/67‑1 (MQ=255)
                                                           agagTAGCGTAGCCAGGCTACCAATTGGGGTAAtttt                                              <  1:3498099/37‑1 (MQ=255)
                                                           agagTAGCGTAGCCAGGCTACCAATTGGGGTAAttt                                               >  1:3833663/1‑36 (MQ=255)
                                                           agagTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATaa            <  1:4839785/71‑1 (MQ=255)
                                                           agagTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATa             >  1:2716251/1‑70 (MQ=255)
                                                             agTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAAc          <  1:3884190/71‑1 (MQ=255)
                                                             agTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAAc          <  1:5198531/71‑1 (MQ=255)
                                                              gtagcgtagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAAc          >  1:5664621/1‑70 (MQ=255)
                                                               tagcgtagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCa                                      <  1:892936/41‑1 (MQ=255)
                                                               tagcgtagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCa                                      <  1:698790/41‑1 (MQ=255)
                                                               tagcgtagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCa                                      <  1:507659/41‑1 (MQ=255)
                                                                    tagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATcac                      <  1:4060700/52‑1 (MQ=255)
                                                                    tagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATcac                      <  1:3148925/52‑1 (MQ=255)
                                                                    tagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATcac                      <  1:3109399/52‑1 (MQ=255)
                                                                    tagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCAt       <  1:2479251/67‑1 (MQ=255)
                                                                    tagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCAt       <  1:5261264/67‑1 (MQ=255)
                                                                     agcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGct   >  1:117541/1‑70 (MQ=255)
                                                                     agcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAACCGCAGCCAATCACATTGGCATAAACCATCGctt  >  1:1749143/1‑71 (MQ=255)
                                                                     |                                                                      
TGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTT  >  Exported/2595383‑2595522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: