Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,595,461 A→G 8.2% G379G (GGT→GGC arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,595,4610AG 8.2% 303.6 / 13.0 109G379G (GGT→GGCarsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base A (53/47);  new base G (6/3);  total (59/50)
Fisher's exact test for biased strand distribution p-value = 5.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.90e-01

Only 100 of 109 total aligned reads displayed.

ATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGAT  >  Exported/2595395‑2595529
                                                                  |                                                                    
atatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCaa                                                                  >  1:2033974/1‑71 (MQ=255)
atatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCa                                                                   <  1:5022952/70‑1 (MQ=255)
 tatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCATAGTAGCGTAGCCAGGCTACCaa                                                                  <  1:4347204/70‑1 (MQ=255)
 tatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAAt                                                                 <  1:2615888/71‑1 (MQ=255)
  atCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAAtt                                                                >  1:2975719/1‑71 (MQ=255)
   tCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTg                                                               >  1:3338200/1‑71 (MQ=255)
    ccccAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTgg                                                              >  1:4586071/1‑71 (MQ=255)
     cccAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTggg                                                             >  1:4068552/1‑71 (MQ=255)
        aGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGt                                                           >  1:2497030/1‑70 (MQ=255)
         gCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTaa                                                         <  1:2839072/71‑1 (MQ=255)
         gCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTaa                                                         <  1:1272417/71‑1 (MQ=255)
           tGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCTAGGCTACCa                                                                   <  1:2456311/59‑1 (MQ=255)
             aTAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAAtt                                                       <  1:4232981/69‑1 (MQ=255)
               aGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAAt                                                                 >  1:4653318/1‑57 (MQ=255)
               aGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCg                                                   >  1:3583917/1‑71 (MQ=255)
               aGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCg                                                   >  1:3891123/1‑71 (MQ=255)
                gTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCg                                                   <  1:5434796/70‑1 (MQ=255)
                 tCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCggg                                                 <  1:1234240/71‑2 (MQ=255)
                   atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTc                                                    >  1:2489482/1‑66 (MQ=255)
                   atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTc                                                    >  1:61438/1‑66 (MQ=255)
                   atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTc                                                    >  1:3968983/1‑66 (MQ=255)
                   atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTc                                                    >  1:866379/1‑66 (MQ=255)
                   atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCg                                                   >  1:1747697/1‑67 (MQ=255)
                   atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTcc                                               >  1:4548297/1‑71 (MQ=255)
                   atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTcc                                               >  1:3251462/1‑71 (MQ=255)
                   atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTcc                                               >  1:3141656/1‑71 (MQ=255)
                    tatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTcc                                               >  1:3336725/1‑70 (MQ=255)
                      ttcttcTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCaa                                            <  1:5141489/71‑1 (MQ=255)
                      ttcttcTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCaa                                            <  1:4267265/71‑1 (MQ=255)
                       tcttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCa                                                                   <  1:1290473/47‑1 (MQ=255)
                       tcttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTccc                                              >  1:1699749/1‑68 (MQ=255)
                       tcttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTccc                                              >  1:1469863/1‑68 (MQ=255)
                         ttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATc                                         >  1:696216/1‑71 (MQ=255)
                          tctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCg                                        >  1:855357/1‑71 (MQ=255)
                             gCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAg                                     >  1:4940678/1‑71 (MQ=255)
                             gCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAg                                     >  1:3120286/1‑71 (MQ=255)
                             gCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAg                                     >  1:2687148/1‑71 (MQ=255)
                              cGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGc                                       <  1:2793191/68‑1 (MQ=255)
                              cGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGc                                       <  1:3526813/68‑1 (MQ=255)
                               gAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATc                                         <  1:3178037/65‑1 (MQ=255)
                               gAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGc                                    >  1:3121949/1‑70 (MQ=255)
                                aaaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCa                                  <  1:1218542/71‑1 (MQ=255)
                                aaaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCa                                  <  1:1807966/71‑1 (MQ=255)
                                 aaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTa                                                          >  1:2402409/1‑46 (MQ=255)
                                 aaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCgg                                                  >  1:1273099/1‑54 (MQ=255)
                                 aaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCg                                                   >  1:3799103/1‑53 (MQ=255)
                                 aaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCaa                                 <  1:1582911/71‑1 (MQ=255)
                                 aaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCaa                                 <  1:4062936/71‑1 (MQ=255)
                                 aaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCaa                                 <  1:3251813/71‑1 (MQ=255)
                                 aaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACAAATTGGGGTAATTTTc                                                    >  1:1483323/1‑52 (MQ=255)
                                  aGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAAt                                >  1:3615097/1‑71 (MQ=255)
                                  aGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAAt                                >  1:4120768/1‑71 (MQ=255)
                                   gTACGTTCAGCCAGAGTAGCGTAGCCAGGCTACCaa                                                                  >  1:732879/1‑36 (MQ=255)
                                   gTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCaa                                                                  >  1:4497734/1‑36 (MQ=255)
                                   gTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCaa                                                                  >  1:1796600/1‑36 (MQ=255)
                                    tACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCaa                                                                  >  1:3714587/1‑35 (MQ=255)
                                    tACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAAt                                                        <  1:5153649/45‑1 (MQ=255)
                                    tACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATca                              >  1:3863892/1‑71 (MQ=255)
                                    tACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATca                              <  1:2745415/71‑1 (MQ=255)
                                    tACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATca                              <  1:2751941/71‑1 (MQ=255)
                                     acatGCAGCCACAGCAGAGTTGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATca                              >  1:1514912/4‑70 (MQ=255)
                                       gTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACAt                           <  1:3790476/71‑1 (MQ=255)
                                       gTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACAt                           <  1:4778729/71‑1 (MQ=255)
                                       atGCAGCCACAGCAGAGTTGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACAt                           <  1:3814260/70‑1 (MQ=255)
                                        tGCAGCCACAGCAGAGTTGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACAt                           >  1:665170/1‑70 (MQ=255)
                                         gCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTg                         >  1:528571/1‑71 (MQ=255)
                                           aGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTgg                        >  1:2543930/1‑70 (MQ=255)
                                            gCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACAtt                          <  1:3803179/67‑1 (MQ=255)
                                              cAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATa                    <  1:1972875/71‑1 (MQ=255)
                                               agagTAGCGTAGCCAGGCTACCAATTGGGGTAAtttt                                                     <  1:3498099/37‑1 (MQ=255)
                                               agagTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATaa                   <  1:4839785/71‑1 (MQ=255)
                                               agagTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATa                    >  1:2716251/1‑70 (MQ=255)
                                                 agTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAAc                 <  1:3884190/71‑1 (MQ=255)
                                                 agTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAAc                 <  1:5198531/71‑1 (MQ=255)
                                                   tagcgtagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCa                                             <  1:698790/41‑1 (MQ=255)
                                                   tagcgtagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCa                                             <  1:507659/41‑1 (MQ=255)
                                                   tagcgtagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCa                                             <  1:892936/41‑1 (MQ=255)
                                                        ttgcCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATcac                             <  1:5693837/50‑1 (MQ=255)
                                                        ttgcCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCAt              >  1:308917/3‑67 (MQ=255)
                                                        ttgcCAGACTGCCAACCGGGGTGATTTTCGGGCCTAAATCACAGCCAATcac                             <  1:5169098/50‑1 (MQ=255)
                                                        tagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATcac                             <  1:4060700/52‑1 (MQ=255)
                                                        tagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATcac                             <  1:3148925/52‑1 (MQ=255)
                                                        tagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATcac                             <  1:3109399/52‑1 (MQ=255)
                                                        tagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCAt              <  1:5261264/67‑1 (MQ=255)
                                                         agcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGct          >  1:117541/1‑70 (MQ=255)
                                                         agcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAACCGCAGCCAATCACATTGGCATAAACCATCGctt         >  1:1749143/1‑71 (MQ=255)
                                                          gcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATa                    <  1:4551350/59‑1 (MQ=255)
                                                          gcCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTg                         >  1:830136/1‑54 (MQ=255)
                                                          gcCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGcctc        >  1:4111743/1‑71 (MQ=255)
                                                          gcCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGcctc        >  1:138197/1‑71 (MQ=255)
                                                           ccAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAAcc                <  1:1278617/62‑1 (MQ=255)
                                                            cAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATaaa                  >  1:725078/1‑59 (MQ=255)
                                                             aGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCttt     <  1:1136169/71‑1 (MQ=255)
                                                               gCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATa                    <  1:5621723/54‑1 (MQ=255)
                                                               gCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATa                    <  1:5274453/54‑1 (MQ=255)
                                                               gCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGa   >  1:3076353/1‑71 (MQ=255)
                                                               gCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGa   >  1:44154/1‑71 (MQ=255)
                                                                cTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATca                              <  1:2849464/43‑1 (MQ=255)
                                                                 tACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCg            >  1:4759544/1‑60 (MQ=255)
                                                                 tACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGAt  >  1:2367820/1‑70 (MQ=255)
                                                                  |                                                                    
ATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGAT  >  Exported/2595395‑2595529

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: