Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,595,467 T→C 16.8% P377P (CCA→CCG arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,595,4670TC16.8% 253.7 / 39.8 113P377P (CCA→CCGarsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base T (49/45);  new base C (11/8);  total (60/53)
Fisher's exact test for biased strand distribution p-value = 8.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01

Only 100 of 118 total aligned reads displayed.

ATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGC  >  Exported/2595397‑2595534
                                                                      |                                                                   
atCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAAtt                                                                     >  1:2975719/1‑71 (MQ=255)
 tCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTg                                                                    >  1:3338200/1‑71 (MQ=255)
  ccccAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTgg                                                                   >  1:4586071/1‑71 (MQ=255)
   cccAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTggg                                                                  >  1:4068552/1‑71 (MQ=255)
      aGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGt                                                                >  1:2497030/1‑70 (MQ=255)
       gCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTaa                                                              <  1:1272417/71‑1 (MQ=255)
       gCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTaa                                                              <  1:2839072/71‑1 (MQ=255)
           aTAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAAtt                                                            <  1:4232981/69‑1 (MQ=255)
             aGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCg                                                        >  1:3583917/1‑71 (MQ=255)
             aGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCg                                                        >  1:3891123/1‑71 (MQ=255)
              gTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCg                                                        <  1:5434796/70‑1 (MQ=255)
               tCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCggg                                                      <  1:1234240/71‑2 (MQ=255)
                 atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTc                                                         >  1:61438/1‑66 (MQ=255)
                 atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTc                                                         >  1:3968983/1‑66 (MQ=255)
                 atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTc                                                         >  1:866379/1‑66 (MQ=255)
                 atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTc                                                         >  1:2489482/1‑66 (MQ=255)
                 atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCg                                                        >  1:1747697/1‑67 (MQ=255)
                 atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTcc                                                    >  1:3251462/1‑71 (MQ=255)
                 atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTcc                                                    >  1:3141656/1‑71 (MQ=255)
                 atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTcc                                                    >  1:4548297/1‑71 (MQ=255)
                  tatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTcc                                                    >  1:4974575/1‑70 (MQ=255)
                  tatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTcc                                                    >  1:3336725/1‑70 (MQ=255)
                    ttcttcTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCaa                                                 <  1:4267265/71‑1 (MQ=255)
                     tcttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTccc                                                   >  1:1699749/1‑68 (MQ=255)
                     tcttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTccc                                                   >  1:1469863/1‑68 (MQ=255)
                      cttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCaaa                                                <  1:4285372/70‑1 (MQ=255)
                       ttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATc                                              >  1:696216/1‑71 (MQ=255)
                        tctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCg                                             >  1:855357/1‑71 (MQ=255)
                           gCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAg                                          >  1:4940678/1‑71 (MQ=255)
                           gCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAg                                          >  1:2687148/1‑71 (MQ=255)
                           gCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAg                                          >  1:3120286/1‑71 (MQ=255)
                            cGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGc                                            <  1:2964023/68‑1 (MQ=255)
                            cGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGc                                            <  1:3526813/68‑1 (MQ=255)
                             gAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATc                                              <  1:3178037/65‑1 (MQ=255)
                             gAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGc                                         >  1:3121949/1‑70 (MQ=255)
                              aaaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCa                                       <  1:1807966/71‑1 (MQ=255)
                              aaaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCa                                       <  1:1218542/71‑1 (MQ=255)
                               aaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTa                                                               >  1:2402409/1‑46 (MQ=255)
                               aaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCg                                                        >  1:3799103/1‑53 (MQ=255)
                               aaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCaa                                      <  1:1582911/71‑1 (MQ=255)
                               aaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCaa                                      <  1:3251813/71‑1 (MQ=255)
                               aaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCaa                                      <  1:4062936/71‑1 (MQ=255)
                               aaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACAAATTGGGGTAATTTTc                                                         >  1:1483323/1‑52 (MQ=255)
                                aGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAAt                                     >  1:4120768/1‑71 (MQ=255)
                                  tACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAAt                                                             <  1:5153649/45‑1 (MQ=255)
                                  tACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATca                                   <  1:2751941/71‑1 (MQ=255)
                                  tACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATca                                   <  1:2745415/71‑1 (MQ=255)
                                   acatGCAGCCACAGCAGAGTTGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATca                                   >  1:1514912/4‑70 (MQ=255)
                                     gTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACAt                                <  1:3790476/71‑1 (MQ=255)
                                     atGCAGCCACAGCAGAGTTGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACAt                                <  1:3814260/70‑1 (MQ=255)
                                      tGCAGCCACAGCAGAGTTGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACAt                                >  1:665170/1‑70 (MQ=255)
                                       gCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTg                              >  1:528571/1‑71 (MQ=255)
                                          gCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACAtt                               <  1:3803179/67‑1 (MQ=255)
                                             agagTAGCGTAGCCAGGCTACCAATTGGGGTAAtttt                                                          <  1:3498099/37‑1 (MQ=255)
                                             agagTAGCGTAGCCAGGCTACCAATTGGGGTAAttt                                                           >  1:3833663/1‑36 (MQ=255)
                                             agagTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATaa                        <  1:4839785/71‑1 (MQ=255)
                                             agagTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATa                         >  1:2716251/1‑70 (MQ=255)
                                               agTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAAc                      <  1:3884190/71‑1 (MQ=255)
                                               agTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAAc                      <  1:5198531/71‑1 (MQ=255)
                                                gtagcgtagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAAc                      >  1:5664621/1‑70 (MQ=255)
                                                 tagcgtagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCa                                                  <  1:507659/41‑1 (MQ=255)
                                                 tagcgtagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCa                                                  <  1:892936/41‑1 (MQ=255)
                                                      ttgcCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATcac                                  <  1:5693837/50‑1 (MQ=255)
                                                      ttgcCAGACTGCCAACCGGGGTGATTTTCGGGCCTAAATCACAGCCAATcac                                  <  1:5169098/50‑1 (MQ=255)
                                                      tagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATcac                                  <  1:3148925/52‑1 (MQ=255)
                                                      tagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATcac                                  <  1:3109399/52‑1 (MQ=255)
                                                      tagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATcac                                  <  1:4060700/52‑1 (MQ=255)
                                                      tagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCAt                   <  1:2479251/67‑1 (MQ=255)
                                                      tagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCAt                   <  1:5261264/67‑1 (MQ=255)
                                                       agcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGct               >  1:117541/1‑70 (MQ=255)
                                                       agcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAACCGCAGCCAATCACATTGGCATAAACCATCGctt              >  1:1749143/1‑71 (MQ=255)
                                                        gcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATa                         <  1:4551350/59‑1 (MQ=255)
                                                        gcCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTg                              >  1:830136/1‑54 (MQ=255)
                                                        gcCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGcctc             >  1:4111743/1‑71 (MQ=255)
                                                        gcCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGcctc             >  1:138197/1‑71 (MQ=255)
                                                         ccAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAAcc                     <  1:1278617/62‑1 (MQ=255)
                                                          cAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATaaa                       >  1:725078/1‑59 (MQ=255)
                                                           aGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCttt          <  1:1136169/71‑1 (MQ=255)
                                                             gCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATa                         <  1:5274453/54‑1 (MQ=255)
                                                             gCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATa                         <  1:5621723/54‑1 (MQ=255)
                                                             gCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGa        >  1:44154/1‑71 (MQ=255)
                                                             gCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGa        >  1:3076353/1‑71 (MQ=255)
                                                              ctgccAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGcctct            >  1:2284202/4‑66 (MQ=255)
                                                              ctgccAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGcctct            >  1:445603/4‑66 (MQ=255)
                                                              ctgccAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGcctct            >  1:5456389/4‑66 (MQ=255)
                                                              ctgccAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGcctct            >  1:3569147/4‑66 (MQ=255)
                                                              cTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATca                                   <  1:2849464/43‑1 (MQ=255)
                                                               tgccAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATca                                   <  1:4793352/40‑1 (MQ=16)
                                                               tgccAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATca                                   <  1:5408309/40‑1 (MQ=16)
                                                               tACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCg                 >  1:4759544/1‑60 (MQ=255)
                                                               tACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGAt       >  1:2367820/1‑70 (MQ=255)
                                                               tACCAATTGGGGTAATTTTAGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGAt       >  1:3327418/1‑70 (MQ=255)
                                                                 ccAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATc                                    >  1:1156533/1‑39 (MQ=255)
                                                                 ccAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAAc    >  1:3451052/1‑71 (MQ=255)
                                                                  cAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATcaca                                 <  1:5446397/41‑1 (MQ=255)
                                                                  cAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATcaca                                 <  1:2562623/41‑1 (MQ=255)
                                                                   aaTCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCa                    >  1:1377362/1‑53 (MQ=255)
                                                                   aaTCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGc  <  1:2905130/71‑1 (MQ=255)
                                                                     ttGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAAcc                     <  1:5342575/50‑1 (MQ=255)
                                                                     ttGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGc  >  1:3748577/1‑69 (MQ=255)
                                                                      |                                                                   
ATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGC  >  Exported/2595397‑2595534

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: