Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,595,571 C→T 21.8% V343I (GTT→ATT)  arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,595,5710CT21.8% 194.5 / 47.8 101V343I (GTT→ATT) arsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base C (41/38);  new base T (8/14);  total (49/52)
Fisher's exact test for biased strand distribution p-value = 2.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.63e-01

Only 100 of 110 total aligned reads displayed.

ACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTC  >  Exported/2595501‑2595638
                                                                      |                                                                   
acaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAc                                                                     >  1:157761/1‑71 (MQ=255)
acaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAc                                                                     >  1:2308503/1‑71 (MQ=255)
acaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAc                                                                     >  1:3459158/1‑71 (MQ=255)
acaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAc                                                                     <  1:1037860/71‑1 (MQ=255)
acaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAc                                                                     <  1:5472608/71‑1 (MQ=255)
 caTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAcc                                                                    <  1:1634087/71‑1 (MQ=255)
 caTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAc                                                                     <  1:1966156/70‑1 (MQ=255)
  aTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCa                                                                   <  1:4849085/71‑1 (MQ=255)
   ttGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAg                                                                  <  1:3909527/71‑1 (MQ=255)
   ttGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAg                                                                  <  1:1979696/71‑1 (MQ=255)
     ggCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGt                                                                 <  1:4262823/70‑1 (MQ=255)
     ggCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGt                                                                 <  1:1472548/70‑1 (MQ=255)
     ggCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGt                                                                 <  1:1822723/70‑1 (MQ=255)
      gCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTa                                                                <  1:2396974/70‑1 (MQ=255)
      gCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTa                                                                <  1:3422995/70‑1 (MQ=255)
         tAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGt                                                            >  1:1124572/1‑71 (MQ=255)
          aaaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTAc                                                               >  1:1088319/1‑67 (MQ=255)
          aaaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTAc                                                               >  1:1249439/1‑67 (MQ=255)
          aaaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc                                                           >  1:2560031/1‑71 (MQ=255)
           aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc                                                           <  1:4005571/70‑1 (MQ=255)
           aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCg                                                          >  1:190643/1‑71 (MQ=255)
           aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCg                                                          >  1:1911825/1‑71 (MQ=255)
           aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCg                                                          >  1:4571849/1‑71 (MQ=255)
           aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCg                                                          >  1:4005558/1‑71 (MQ=255)
           aaCCATCGCTTCTTTGATAACGCCAGATGCCGAGCTGCCATCAATGGACAACGCGCCAAcc                                                                    >  1:4243777/1‑61 (MQ=255)
                  gcctcttTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCatat                                                    >  1:300896/4‑70 (MQ=255)
                  gCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATAtt                                                   >  1:285528/1‑71 (MQ=255)
                  gCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATAtt                                                   <  1:4176290/71‑1 (MQ=255)
                       tttGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGtt                                                <  1:1311838/69‑1 (MQ=255)
                         tGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGt                                                            >  1:389031/1‑55 (MQ=255)
                         tGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCat                                             <  1:278853/70‑1 (MQ=255)
                          gATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCataa                                           >  1:467426/1‑71 (MQ=255)
                           aTAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGt                                                 >  1:5610185/1‑64 (MQ=255)
                            cgaCGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTc                                               <  1:2852098/63‑1 (MQ=255)
                              aCGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCat                                             <  1:5301797/65‑1 (MQ=255)
                              aCGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATaga                                       <  1:1448564/71‑1 (MQ=255)
                              aCGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATaga                                       <  1:1441744/71‑1 (MQ=255)
                              aCGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATag                                        <  1:5685742/70‑1 (MQ=255)
                              aCGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATag                                        <  1:364533/70‑1 (MQ=255)
                              aCGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATag                                        <  1:4980414/70‑1 (MQ=255)
                              aCGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATag                                        <  1:1061604/70‑1 (MQ=255)
                               cGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaa                                      <  1:3890566/71‑1 (MQ=255)
                               cGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaa                                      <  1:464555/71‑1 (MQ=255)
                                gCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCatca                                           >  1:1893540/1‑63 (MQ=255)
                                gCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCa                                                                   >  1:2004573/1‑41 (MQ=255)
                                gCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCa                                                                   >  1:2674055/1‑41 (MQ=255)
                                gCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCa                                                                   >  1:1560367/1‑41 (MQ=255)
                                gCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaag                                     <  1:1003764/71‑1 (MQ=255)
                                 ccAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTAcc                                                              <  1:2483012/45‑1 (MQ=255)
                                 ccAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaaga                                    <  1:1625058/71‑1 (MQ=255)
                                    gATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAagagag                                 <  1:5283863/71‑1 (MQ=255)
                                       gCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc                                                           >  1:3029323/1‑42 (MQ=255)
                                       gCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc                                                           >  1:5695721/1‑42 (MQ=255)
                                       gCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc                                                           >  1:5313032/1‑42 (MQ=255)
                                       gCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc                                                           >  1:1142167/1‑42 (MQ=255)
                                       gCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc                                                           >  1:1026562/1‑42 (MQ=255)
                                       gCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTg                                                  >  1:3789575/1‑51 (MQ=255)
                                       gCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGt                                                 >  1:2675742/1‑52 (MQ=255)
                                        ccGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGtt                                                >  1:4158798/1‑52 (MQ=255)
                                        ccGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCat                                             <  1:4648604/55‑1 (MQ=255)
                                        ccGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCat                                             <  1:1134433/55‑1 (MQ=255)
                                         cGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGaaatg                            >  1:4332174/1‑69 (MQ=255)
                                           tgctgcCATCAATGGACAACGCGCCAACCAGTACCGTCgg                                                         <  1:3779870/40‑1 (MQ=255)
                                           tgctgcCATCAATGGACAACGCGCCAACCAGTACCGTCgg                                                         <  1:2187126/40‑1 (MQ=255)
                                           tgctgcCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCg                          <  1:2098620/71‑1 (MQ=255)
                                             ctgcCATCAATGGACAACGCGCCAACCAGTACCGTCGGCat                                                      >  1:569487/1‑41 (MQ=255)
                                                acATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAAAAGAAGAGAGGAAGGCGGTGAg                     >  1:2566778/2‑71 (MQ=255)
                                                 cgtCAATCGACAGCGCACCAATCAGTACCGTCGGCATATTGTTCat                                             >  1:1992271/3‑46 (MQ=25)
                                                 cgtCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCat                                             >  1:566624/3‑46 (MQ=255)
                                                 cATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCataa                                           >  1:3032727/1‑48 (MQ=255)
                                                   tCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTg                                                  <  1:1539955/39‑1 (MQ=255)
                                                   tCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTg                                                  <  1:3412685/39‑1 (MQ=255)
                                                   tCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGaa                  <  1:4671686/71‑1 (MQ=255)
                                                    cAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAAt                 >  1:606189/1‑71 (MQ=255)
                                                     aaTGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCata                                            >  1:2090024/1‑43 (MQ=255)
                                                     aaTGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCata                                            >  1:2973408/1‑43 (MQ=255)
                                                     aaTGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCata                                            >  1:1973001/1‑43 (MQ=255)
                                                     aaTGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCata                                            >  1:1108406/1‑43 (MQ=255)
                                                     aaTGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAagag                                   >  1:2789313/1‑52 (MQ=255)
                                                     aaTCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGaagc                <  1:4929070/71‑3 (MQ=255)
                                                     aaTCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGaagc                <  1:3490046/71‑3 (MQ=255)
                                                       tGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATag                                        <  1:3300775/45‑1 (MQ=255)
                                                       tGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATag                                        <  1:4363712/45‑1 (MQ=255)
                                                       tGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATag                                        <  1:3637119/45‑1 (MQ=255)
                                                       tGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATag                                        <  1:2407353/45‑1 (MQ=255)
                                                       tGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGt                        <  1:218872/61‑1 (MQ=255)
                                                         gACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGt            <  1:3523388/71‑1 (MQ=255)
                                                         gACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGaa                              >  1:1057806/1‑53 (MQ=255)
                                                           cAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGc          >  1:3578976/1‑71 (MQ=255)
                                                             gcgcaCCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCg        <  1:4148179/70‑1 (MQ=255)
                                                             gcgcaCCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCg        <  1:3499369/70‑1 (MQ=255)
                                                              cgcgCCAACCAGTACCGTCGGCATATTGTTCATAATagaaga                                    >  1:5598692/1‑42 (MQ=255)
                                                              cgcgCCAACCAGTACCGTCGGCATATTGTTCATAATagaaga                                    >  1:800238/1‑42 (MQ=255)
                                                              cgcgCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCgg             >  1:1755250/1‑65 (MQ=255)
                                                                 accAATCAGCACCGTCGGCATATTGTTCATGACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGaac     <  1:4954810/69‑3 (MQ=255)
                                                                 accAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACg    >  1:5332894/2‑71 (MQ=255)
                                                                   cAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTc  <  1:5586629/71‑1 (MQ=255)
                                                                   cAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGt   <  1:4575829/70‑1 (MQ=255)
                                                                   cAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTc  >  1:5083682/1‑71 (MQ=255)
                                                                    aaCCAGTACCGTCGGCATATTGTTCATAATagaaga                                    <  1:579353/36‑1 (MQ=255)
                                                                      |                                                                   
ACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTC  >  Exported/2595501‑2595638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: